Protein Alignment Raf and Araf

DIOPT Version :9

• Sequence 1: NP_001096867.3
• Gene: Raf / 31221
• FlyBaseID: FBgn0003079
• Length: 739
• Species: Drosophila melanogaster
• Sequence 2: XP_011245731.2
• Gene: Araf / 11836
• MGIID: 88065
• Length: 674
• Species: Mus musculus
• Alignment Length: 637
• Identity: 289/637 - (45%)
• Similarity: 374/637 - (58%)
• Gaps: 94/637 - (14%)

  Fly   125 GSQPGSQCGTLTRQPKILLRAHLPNQQRTSVEVISGVRLCDALMKALKLRQLTPDMCEVSTTHSG 189
            |::|....||        ::.:|||:|||.|.|..|:.:.|:|.||||:|.|..|.|.|.....|
Mouse    81 GAEPSRAVGT--------VKVYLPNKQRTVVTVREGMSVYDSLDKALKVRGLNQDCCVVYRLIKG 137

  Fly   190 RH-IIPWHTDIGTLHVEEIFVRLLDKFPIRTHIKHQIIRKTFFSLVFCEGCRRLLFTGFYCSQCN 253
            |. :..|.|.|..|..||:.|.:|:..|:   ..|..:|||||||.||:.|.:.||.||.|..|.
Mouse   138 RKTVTAWDTAIAPLDGEELIVEVLEDVPL---TMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCG 199

  Fly   254 FRFHQRCANRVPMLCQPFPMDSYYQLLLAENPDNGVGFPGRGTAVRFNMSSRSRSRRCS---SSG 315
            ::|||.|:::||.:|                               .:||:..|....|   .||
Mouse   200 YKFHQHCSSKVPTVC-------------------------------VDMSTNRRQFYHSIQDLSG 233

  Fly   316 SSSSSKPPSSSSGNH---RQGRPPRISQDD-----------------RSNSAPNVCINNIRSVTS 360
            .|...:.||:.|.|.   .||..|...|.|                 ||.|.|||   ::.|.|:
Mouse   234 GSRQQEAPSNLSVNELLTPQGPSPFTQQRDQEHFSFPAPANPPLQRIRSTSTPNV---HMVSTTA 295

  Fly   361 EVQRSLIMQAR--------------PPLPHPCTDHSNSTQASPTSTL-KHNRPRARSADESN--K 408
            .:..||:....              .|...|.....:|.:.||.|.| ...|.|...|||..  |
Mouse   296 PMDSSLMQFTAQSFSTDAAGRGGDGAPRGSPSPASVSSGRKSPHSKLPSEQRERKSLADEKKKVK 360

  Fly   409 NLLLRDAKSSEENWNILAEEILIGPRIGSGSFGTVYRAHWHGPVAVKTLNVKTPSPAQLQAFKNE 473
            ||..||   |...|.:...|:.:..|||:||||||:|..|||.||||.|.|..|:..|.||||||
Mouse   361 NLGYRD---SGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVAQPTAEQAQAFKNE 422

  Fly   474 VAMLKKTRHCNILLFMGCVSKPSLAIVTQWCEGSSLYKHVHVSETKFKLNTLIDIGRQVAQGMDY 538
            :.:|:||||.|||||||.:::|..||:||||||||||.|:||::|:|.:..|||:.||.||||||
Mouse   423 MQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDY 487

  Fly   539 LHAKNIIHRDLKSNNIFLHEDLSVKIGDFGLATAKTRWSGEKQANQPTGSILWMAPEVIRMQELN 603
            ||||||||||||||||||||.|:||||||||||.||||||.:...||:||:||||.||||||:.|
Mouse   488 LHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPN 552

  Fly   604 PYSFQSDVYAFGIVMYELLAECLPYGHISNKDQILFMVGRGLLRPDMSQVRSDAPQALKRLAEDC 668
            ||||||||||:|:|:|||:...|||.||.::|||:||||||.|.||:|::.|:.|:|::||..||
Mouse   553 PYSFQSDVYAYGVVLYELMTGSLPYSHIGSRDQIIFMVGRGYLSPDLSKIFSNCPKAMRRLLTDC 617

  Fly   669 IKYTPKDRPLFRPLLNMLENMLRTLPKIHRSASEPNLTQSQLQNDEFLYLPS 720
            :|:..::||||..:|..:|.:.|:||||.||||||:|.::|.  ||   ||:
Mouse   618 LKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQA--DE---LPA 664

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Raf	NP_001096867.3	RBD_RAF	142..212	CDD:340514	29/70 (41%)
		C1_Raf	221..269	CDD:410361	23/47 (49%)
		STKc_Raf	435..687	CDD:270964	165/251 (66%)
Araf	XP_011245731.2	RBD_ARAF	89..161	CDD:340653	31/79 (39%)
		C1_1	169..214	CDD:365894	23/44 (52%)
		PKc_like	377..641	CDD:389743	168/263 (64%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	378	1.000	Domainoid score	I850
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	583	1.000	Inparanoid score	I1002
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG49873
OrthoDB	1	1.010	-	-		D129822at33208
OrthoFinder	1	1.000	-	-		FOG0001587
OrthoInspector	1	1.000	-	-		otm43050
orthoMCL	1	0.900	-	-		OOG6_100006
Panther	1	1.100	-	-	O	PTHR44329
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2599
SonicParanoid	1	1.000	-	-		X994
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	13.010