Protein Alignment Raf and Raf1

DIOPT Version :10

• Sequence 1: NP_525047.1
• Gene: Raf / 31221
• FlyBaseID: FBgn0003079
• Length: 739
• Species: Drosophila melanogaster
• Sequence 2: NP_001343262.1
• Gene: Raf1 / 110157
• MGIID: 97847
• Length: 668
• Species: Mus musculus
• Alignment Length: 675
• Identity: 308/675 - (45%)
• Similarity: 400/675 - (59%)
• Gaps: 81/675 - (12%)

  Fly   114 VHHGNDLTDSLGSQPGSQC------------------GTLTRQPKI--LLRAHLPNQQRTSVEVI 158
            :.:|..|.|::..  ||.|                  |.||...|.  .:|..|||:|||.|.|.
Mouse    11 ISNGFGLKDAVFD--GSSCISPTIVQQFGYQRRASDDGKLTDSSKTSNTIRVFLPNKQRTVVNVR 73

  Fly   159 SGVRLCDALMKALKLRQLTPDMCEV---STTHSGRHI-IPWHTDIGTLHVEEIFVRLLDKFPIRT 219
            :|:.|.|.||||||:|.|.|:.|.|   ...|.|:.. :.|:||..:|..||:.|..||..|:.|
Mouse    74 NGMSLHDCLMKALKVRGLQPECCAVFRLLQEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTT 138

  Fly   220 HIKHQIIRKTFFSLVFCEGCRRLLFTGFYCSQCNFRFHQRCANRVPMLCQPFPMDSYYQLLLAEN 284
               |...||||..|.||:.|::.|..||.|..|.::||:.|:.:||.:|..:  .:..||||..|
Mouse   139 ---HNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDW--SNIRQLLLFPN 198

  Fly   285 P---DNGV----GFPGRGTAVRFNMSSRSRSRRCSSSGSSSSSKPPSSSSGNHRQGRPPRISQDD 342
            .   |:||    .||.|......:....|...|.|:..:.:.:....||.|:        :||..
Mouse   199 STVGDSGVPAPPSFPMRRMRESVSRMPASSQHRYSTPHAFTFNTSSPSSEGS--------LSQRQ 255

  Fly   343 RSNSAPNV-CINNIRSVTSEVQRSLIMQARPPLPHPCT------------DHSNSTQASPTSTLK 394
            ||.|.||| .::....|.|.:..|..:.|.   |..|:            .||.|...|..|:..
Mouse   256 RSTSTPNVHMVSTTLHVDSRMIESNSLNAS---PRACSRRFCLRGRDAIRSHSESASPSALSSSP 317

  Fly   395 HN----------------RPRARSADESNKNLLL-RDAKSSEENWNILAEEILIGPRIGSGSFGT 442
            :|                |.||..:....||.:. |..:.|...|.|.|.|:::..|||||||||
Mouse   318 NNLSPTGWSQPKTPVPAQRERAPGSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGT 382

  Fly   443 VYRAHWHGPVAVKTLNVKTPSPAQLQAFKNEVAMLKKTRHCNILLFMGCVSKPSLAIVTQWCEGS 507
            ||:..|||.||||.|.|..|:|.|||||:||||:|:||||.|||||||.::|.:|||||||||||
Mouse   383 VYKGKWHGDVAVKILKVVDPTPEQLQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGS 447

  Fly   508 SLYKHVHVSETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHEDLSVKIGDFGLATA 572
            |||||:||.||||::..||||.||.||||||||||||||||:|||||||||.|:||||||||||.
Mouse   448 SLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATV 512

  Fly   573 KTRWSGEKQANQPTGSILWMAPEVIRMQELNPYSFQSDVYAFGIVMYELLAECLPYGHISNKDQI 637
            |:||||.:|..|||||:|||||||||||:.||:|||||||::|||:|||:|..|||.||:|:|||
Mouse   513 KSRWSGSQQVEQPTGSVLWMAPEVIRMQDDNPFSFQSDVYSYGIVLYELMAGELPYAHINNRDQI 577

  Fly   638 LFMVGRGLLRPDMSQVRSDAPQALKRLAEDCIKYTPKDRPLFRPLLNMLENMLRTLPKIHRSASE 702
            :||||||...||:|::..:.|:|:|||..||:|...::||||..:|:.:|.:..:||||:|||||
Mouse   578 IFMVGRGYASPDLSRLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASE 642

  Fly   703 PNLTQSQLQND--EFLYLPSPKTPV 725
            |:|.::....|  ......||:.||
Mouse   643 PSLHRAAHTEDINACTLTTSPRLPV 667

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Raf	NP_525047.1	RBD_RAF	142..212	CDD:340514	32/73 (44%)
		C1_Raf	221..269	CDD:410361	19/47 (40%)
		STKc_Raf	435..687	CDD:270964	176/251 (70%)
Raf1	NP_001343262.1	RBD_CRAF	55..131	CDD:340655	32/75 (43%)
		C1_A_C-Raf	136..187	CDD:410420	21/53 (40%)
		STKc_C-Raf	356..638	CDD:271051	187/281 (67%)