DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Raf and Braf

DIOPT Version :9

Sequence 1:	NP_001096867.3	Gene:	Raf / 31221	FlyBaseID:	FBgn0003079	Length:	739	Species:	Drosophila melanogaster
Sequence 2:	XP_011239436.1	Gene:	Braf / 109880	MGIID:	88190	Length:	917	Species:	Mus musculus

Alignment Length:	823	Identity:	355/823 - (43%)
Similarity:	462/823 - (56%)	Gaps:	135/823 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 SDLYDP-LAEELHNVQLVKHVTRENIDALNAKFANLQEPPAMYLIEYQELTSKLHELEAKEQELM 74
            |...|| :.||:.|::.:..:|:|:|:||..||.....||::||..|:|.||||..|:.:||:|:
Mouse   130 SSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLL 194

  Fly    75 ERLNSQDQQEDSSLVERFKEQPHYQNQTQILQQQRQLARVHHGNDLTDSLGSQPGSQCGTLTRQP 139
            |.|.:......||............:...:|.....:.:..     ||:..:.|.|       ..
Mouse   195 ESLGNGADFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQTP-----TDASRNNPKS-------PQ 247

  Fly   140 KILLRAHLPNQQRTSVEVISGVRLCDALMKALKLRQLTPDMCEVSTTHSG----RHIIPWHTDIG 200
            |.::|..|||:|||.|....||.:.|:|.|||.:|.|.|:.|.|.....|    :..|.|.|||.
Mouse   248 KPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGYGLEKKPIGWDTDIS 312

  Fly   201 TLHVEEIFVRLLDKFPIRTHIKHQIIRKTFFSLVFCEGCRRLLFTGFYCSQCNFRFHQRCANRVP 265
            .|..||:.|.:|:..|:.|   |..:|||||:|.||:.||:|||.||.|..|.::|||||:..||
Mouse   313 WLTGEELHVEVLENVPLTT---HNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVP 374

  Fly   266 MLCQPF-PMDSYYQLLLAEN---PDNGVGFPGRGTAVRFNMSSRSRSRRCSSSGSSSSSKPPSSS 326
            ::|..: .:|..:.....|:   |.....||  .||:                .|.|||.|||.|
Mouse   375 LMCVNYDQLDLLFVSKFFEHHPVPQEEASFP--ETAL----------------PSGSSSAPPSDS 421

  Fly   327 SG------------------------NHRQGRPPRISQDDRSNSAPNVCINNIRSVT-----SEV 362
            :|                        :||.    :..|.|||:|||||.||.|..|.     .||
Mouse   422 TGPQILTSPSPSKSIPIPQPFRPADEDHRN----QFGQRDRSSSAPNVHINTIEPVNIDEKFPEV 482

  Fly   363 ----QRSLI---------------------MQARPPLP-------------HPCTDHSNST---Q 386
                ||.||                     .|:|.|.|             .|......||   .
Mouse   483 ELQDQRDLIRDQGFRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTTGLS 547

  Fly   387 ASPTSTL-----------------KHNRPRARSADESNKNLLLRDAKSSEENWNILAEEILIGPR 434
            |:|.::|                 :..:..:.|:.|....:.....:.|.::|.|...:|.:|.|
Mouse   548 ATPPASLPGSLTNVKALQKSPGPQRERKSSSSSSSEDRSRMKTLGRRDSSDDWEIPDGQITVGQR 612

  Fly   435 IGSGSFGTVYRAHWHGPVAVKTLNVKTPSPAQLQAFKNEVAMLKKTRHCNILLFMGCVSKPSLAI 499
            ||||||||||:..|||.||||.|||..|:|.|||||||||.:|:||||.|||||||..:||.|||
Mouse   613 IGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAI 677

  Fly   500 VTQWCEGSSLYKHVHVSETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHEDLSVKI 564
            |||||||||||.|:|:.||||::..||||.||.||||||||||:|||||||||||||||||:|||
Mouse   678 VTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKI 742

  Fly   565 GDFGLATAKTRWSGEKQANQPTGSILWMAPEVIRMQELNPYSFQSDVYAFGIVMYELLAECLPYG 629
            |||||||.|:||||..|..|.:||||||||||||||:.|||||||||||||||:|||:...|||.
Mouse   743 GDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYS 807

  Fly   630 HISNKDQILFMVGRGLLRPDMSQVRSDAPQALKRLAEDCIKYTPKDRPLFRPLLNMLENMLRTLP 694
            :|:|:|||:||||||.|.||:|:|||:.|:|:|||..:|:|....:||||..:|..:|.:.|:||
Mouse   808 NINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLP 872

  Fly   695 KIHRSASEPNLTQSQLQNDEF-LY-LPSPKTPVNFNNFQFFGS 735
            |||||||||:|.::..|.::| || ..|||||:....:..|.:
Mouse   873 KIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAA 915

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Raf	NP_001096867.3	RBD_RAF	142..212	CDD:340514	30/73 (41%)
		C1_Raf	221..269	CDD:410361	25/47 (53%)
		STKc_Raf	435..687	CDD:270964	180/251 (72%)
Braf	XP_011239436.1	RBD_BRAF	248..329	CDD:340654	32/80 (40%)
		C1_1	332..377	CDD:365894	25/44 (57%)
		STKc_Raf	613..865	CDD:270964	180/251 (72%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	378	1.000	Domainoid score	I850
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H3197
Inparanoid	1	1.050	583	1.000	Inparanoid score	I1002
Isobase	1	0.950	-	0	Normalized mean entropy	S2247
OMA	1	1.010	-	-		QHG49873
OrthoDB	1	1.010	-	-		D129822at33208
OrthoFinder	1	1.000	-	-		FOG0001587
OrthoInspector	1	1.000	-	-		otm43050
orthoMCL	1	0.900	-	-		OOG6_100006
Panther	1	1.100	-	-	LDO	PTHR44329
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2599
SonicParanoid	1	1.000	-	-		X994
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	15	14.960