Protein Alignment Grp170 and KAR2

DIOPT Version :10

• Sequence 1: NP_569995.1
• Gene: Grp170 / 31215
• FlyBaseID: FBgn0023529
• Length: 923
• Species: Drosophila melanogaster
• Sequence 2: NP_012500.3
• Gene: KAR2 / 853418
• SGDID: S000003571
• Length: 682
• Species: Saccharomyces cerevisiae
• Alignment Length: 769
• Identity: 194/769 - (25%)
• Similarity: 322/769 - (41%)
• Gaps: 159/769 - (20%)

  Fly     2 KLVLLLSALLAGI---------------ALSQGA-------AVMSVDLGSEWMKVGVVSPGVPME 44
            ||::.||.:|..:               .|.:||       .|:.:|||:.:..|.|:..| ..|
Yeast    10 KLLVPLSVVLYALFVVILPLQNSFHSSNVLVRGADDVENYGTVIGIDLGTTYSCVAVMKNG-KTE 73

  Fly    45 IALNRESKRKTPAILAFRDGTRTIGEDAQTIGIKDPNSAYGYLLDLLGKTIDNPIVDLYRKRFPY 109
            |..|.:..|.||:.:||.|..|.||:.|:.....:|.:....:..|:|...::..|....|..|
Yeast    74 ILANEQGNRITPSYVAFTDDERLIGDAAKNQVAANPQNTIFDIKRLIGLKYNDRSVQKDIKHLP- 137

  Fly   110 YNIVGDPERNTV-VFRKSDTDEFSVEELVAQLLVKAKQFAQESVQQPITECVLTVPGYFGQAERE 173
            :|:|....:..| |..|.:...|:.||:...:|.|.||.|::.:...:|..|:|||.||..|:|:
Yeast   138 FNVVNKDGKPAVEVSVKGEKKVFTPEEISGMILGKMKQIAEDYLGTKVTHAVVTVPAYFNDAQRQ 202

  Fly   174 ALLSAAQLANLKVLQLINDYAAVALNYGVFHRGEINETAQYFLFYDMGAYKTSAAVVSYQLVKDK 238
            |...|..:|.|.||:::|:..|.|:.||:    :.::.....:.||:|......:::|.:
Yeast   203 ATKDAGTIAGLNVLRIVNEPTAAAIAYGL----DKSDKEHQIIVYDLGGGTFDVSLLSIE----- 258

  Fly   239 QTREINPVVQVLGVGYDRTLGGLEIQLRLRDYLAQEFNALKKTKTDVTTSPRALAKLFKEAGRLK 303
                 |.|.:|.....|..|||.:...::...|.:.|.  ||...||:.:.:|||||.:||.:.|
Yeast   259 -----NGVFEVQATSGDTHLGGEDFDYKIVRQLIKAFK--KKHGIDVSDNNKALAKLKREAEKAK 316

  Fly   304 NVLSANTEFFAQIENLIEDIDFKLPVTREKLEQLCEDLWPRATKPLEEALASSHLSLDVINQVIL 368
            ..||:......:|::.::.||....:||.|.|:|..||:.:..||:|:.|..|.|....::.::|
Yeast   317 RALSSQMSTRIEIDSFVDGIDLSETLTRAKFEELNLDLFKKTLKPVEKVLQDSGLEKKDVDDIVL 381

  Fly   369 FGGGTRVPRVQETIKAVIK-QELGKNLNADESATMGAVYKAADLSAGFKVKKFVVKDATLFPLQV 432
            .||.||:|:||:.:::... ::..|.:|.||:...||..:|..||....|:..|:.|.....|.:
Yeast   382 VGGSTRIPKVQQLLESYFDGKKASKGINPDEAVAYGAAVQAGVLSGEEGVEDIVLLDVNALTLGI 446

  Fly   433 SFERDPGDGAAVKQVKRALFALMNPYPQKKVITFNKHTDDF-EFYVNYADLDRYSKEEIAALGSL 496
                :...|.....:||     ....|.||...|:...|:. ...:...:.:|...::...||..
Yeast   447 ----ETTGGVMTPLIKR-----NTAIPTKKSQIFSTAVDNQPTVMIKVYEGERAMSKDNNLLGKF 502

  Fly   497 NVTKV--QLKQVKELLEKSKKELVDNKGIKAYFYLDDSGIFRCTGVEYVYEKQKPEDDADEDSTL 559
            .:|.:  ..:.|.:              |:..|.||.:||.:.:              |.:..|
Yeast   503 ELTGIPPAPRGVPQ--------------IEVTFALDANGILKVS--------------ATDKGT- 538

  Fly   560 SKFGSTLSKLFTKEGEEKKDNSEQEEAANAGEEPSKSEDNEKAKEEDASKEQKSEESTKQDTEAK 624
               |.:.|...|.:    |....|||.....||.      ||...||||
Yeast   539 ---GKSESITITND----KGRLTQEEIDRMVEEA------EKFASEDAS---------------- 574

  Fly   625 NETIKLVTVKSPVTYESQTQFVVPLVGSAYDQSVAKLAAINKAEEQRVRLESAFNALEAHIIEVQ 689
               ||                             ||:.:.||.|.....|::..|.      ::.
Yeast   575 ---IK-----------------------------AKVESRNKLENYAHSLKNQVNG------DLG 601

  Fly   690 QKLDEESYAKCATAEEKEKLLAECSTLGEWLYEDLEDPKAEIYEEKLAQLKKLS 743
            :||:|         |:||.||...:.:.|||.::.|...||.::||...|.|::
Yeast   602 EKLEE---------EDKETLLDAANDVLEWLDDNFETAIAEDFDEKFESLSKVA 646

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Grp170	NP_569995.1	ASKHA_NBD_HSP70_HYOU1	21..409	CDD:466828	116/389 (30%)
		PTZ00121	<546..917	CDD:173412	44/198 (22%)
		TAF4	<572..>627	CDD:461598	12/54 (22%)
KAR2	NP_012500.3	HSP70	52..654	CDD:394970	186/727 (26%)