Protein Alignment Grp170 and Hspa8

DIOPT Version :10

• Sequence 1: NP_569995.1
• Gene: Grp170 / 31215
• FlyBaseID: FBgn0023529
• Length: 923
• Species: Drosophila melanogaster
• Sequence 2: NP_112442.2
• Gene: Hspa8 / 15481
• MGIID: 105384
• Length: 646
• Species: Mus musculus
• Alignment Length: 756
• Identity: 190/756 - (25%)
• Similarity: 333/756 - (44%)
• Gaps: 163/756 - (21%)

  Fly    16 LSQGAAVMSVDLGSEWMKVGVVSPGVPMEIALNRESKRKTPAILAFRDGTRTIGEDAQTIGIKDP 80
            :|:|.|| .:|||:.:..|||...| .:||..|.:..|.||:.:||.|..|.||:.|:.....:|
Mouse     1 MSKGPAV-GIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 63

  Fly    81 NSAYGYLLDLLGKTIDNPIVDLYRKRFPYYNIVGDPERNTV-VFRKSDTDEFSVEELVAQLLVKA 144
            .:.......|:|:..|:.:|....|.:|:. :|.|..|..| |..|.:|..|..||:.:.:|.|.
Mouse    64 TNTVFDAKRLIGRRFDDAVVQSDMKHWPFM-VVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKM 127

  Fly   145 KQFAQESVQQPITECVLTVPGYFGQAEREALLSAAQLANLKVLQLINDYAAVALNYGVFHR--GE 207
            |:.|:..:.:.:|..|:|||.||..::|:|...|..:|.|.||::||:..|.|:.||:..:  .|
Mouse   128 KEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAE 192

  Fly   208 INETAQYFLFYDMGAYKTSAAVVSYQLVKDKQTREINPVVQVLGVGYDRTLGGLEIQLRLRDYLA 272
            .|     .|.:|:|......::::.:          :.:.:|.....|..|||.:...|:.::..
Mouse   193 RN-----VLIFDLGGGTFDVSILTIE----------DGIFEVKSTAGDTHLGGEDFDNRMVNHFI 242

  Fly   273 QEFNALKKTKTDVTTSPRALAKLFKEAGRLKNVLSANTEFFAQIENLIEDIDFKLPVTREKLEQL 337
            .||.  :|.|.|::.:.||:.:|.....|.|..||::|:...:|::|.|.|||...:||.:.|:|
Mouse   243 AEFK--RKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEEL 305

  Fly   338 CEDLWPRATKPLEEALASSHLSLDVINQVILFGGGTRVPRVQETIKAVIK-QELGKNLNADESAT 401
            ..||:.....|:|:||..:.|....|:.::|.||.||:|::|:.::.... :||.|::|.||:..
Mouse   306 NADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVA 370

  Fly   402 MGAVYKAADLSAGFK---VKKFVVKDATLFPLQVSFERDPGDGAAVKQVKRALFALMNPYPQKKV 463
            .||..:||.|| |.|   |:..::.|.|  ||.:..|...|       |...|.......|.|:.
Mouse   371 YGAAVQAAILS-GDKSENVQDLLLLDVT--PLSLGIETAGG-------VMTVLIKRNTTIPTKQT 425

  Fly   464 ITFNKHTDD-----FEFYVNYADLDRYSKEEIAALGSLNVTKVQ-----LKQVKELLEKSKKELV 518
            .||..::|:     .:.|    :.:|...::...||...:|.:.     :.|::...:      :
Mouse   426 QTFTTYSDNQPGVLIQVY----EGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFD------I 480

  Fly   519 DNKGIKAYFYLDDSGIFRCTGVEYVYEKQKPEDDADEDSTLSKFGSTLSKLFTKEGEEKKDNSEQ 583
            |..||     |:.|.:.:.||.|                  :|...|..|     |...|::.|:
Mouse   481 DANGI-----LNVSAVDKSTGKE------------------NKITITNDK-----GRLSKEDIER 517

  Fly   584 --EEAANAGEEPSKSEDNEKAKEEDASKEQKSEESTKQDTEAKNETIKLVTVKSPVTYESQTQFV 646
              :||             ||.|.||
Mouse   518 MVQEA-------------EKYKAED---------------------------------------- 529

  Fly   647 VPLVGSAYDQSVAKLAAINKAEEQRVRLESAFNALEAHIIEVQQKLDEESYAKCATAEEKEKLLA 711
                                 |:||.::.|. |:||::...::..:::|........|:|:|:|.
Mouse   530 ---------------------EKQRDKVSSK-NSLESYAFNMKATVEDEKLQGKINDEDKQKILD 572

  Fly   712 ECSTLGEWLYEDLEDPKAEIYEEKLAQLKKLSNVFLARHWE 752
            :|:.:..||.::....|.| :|.:..:|:|:.|..:.:.::
Mouse   573 KCNEIISWLDKNQTAEKEE-FEHQQKELEKVCNPIITKLYQ 612

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Grp170	NP_569995.1	ASKHA_NBD_HSP70_HYOU1	21..409	CDD:466828	120/391 (31%)
		PTZ00121	<546..917	CDD:173412	34/209 (16%)
		TAF4	<572..>627	CDD:461598	10/56 (18%)
Hspa8	NP_112442.2	PTZ00009	1..613	CDD:240227	190/756 (25%)
		Nucleotide-binding domain (NBD). /evidence=ECO:0000250|UniProtKB:P11142	2..386		128/404 (32%)
		Interaction with BAG1. /evidence=ECO:0000250	186..377		57/207 (28%)
		Substrate-binding domain (SBD). /evidence=ECO:0000250|UniProtKB:P11142	394..509		30/161 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	614..646