DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG2918 and Hspa5

DIOPT Version :9

Sequence 1:	NP_001162645.1	Gene:	CG2918 / 31215	FlyBaseID:	FBgn0023529	Length:	923	Species:	Drosophila melanogaster
Sequence 2:	NP_001156906.1	Gene:	Hspa5 / 14828	MGIID:	95835	Length:	655	Species:	Mus musculus

Alignment Length:	774	Identity:	193/774 - (24%)
Similarity:	323/774 - (41%)	Gaps:	172/774 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MKLVLLLSA-LLAGIALSQG-------AAVMSVDLGSEWMKVGVVSPGVPMEIALNRESKRKTPA 57
            ||..::.:| ||.|...::.       ..|:.:|||:.:..|||...| .:||..|.:..|.||:
Mouse     2 MKFTVVAAALLLLGAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNG-RVEIIANDQGNRITPS 65

  Fly    58 ILAFR-DGTRTIGEDAQTIGIKDPNSAYGYLLDLLGKTIDNPIVDLYRKRFPYYNIVGDPERNTV 121
            .:||. :|.|.||:.|:.....:|.:.......|:|:|.::|.|....|..|:..:    |:.|.
Mouse    66 YVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVV----EKKTK 126

  Fly   122 VFRKSD-----TDEFSVEELVAQLLVKAKQFAQESVQQPITECVLTVPGYFGQAEREALLSAAQL 181
            .:.:.|     |..|:.||:.|.:|.|.|:.|:..:.:.:|..|:|||.||..|:|:|...|..:
Mouse   127 PYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 191

  Fly   182 ANLKVLQLINDYAAVALNYGVFHR-GEINETAQYFLFYDMGAYKTSAAVVSYQLVKDKQTREINP 245
            |.|.|:::||:..|.|:.||:..| ||.|     .|.:|:|......::::..          |.
Mouse   192 AGLNVMRIINEPTAAAIAYGLDKREGEKN-----ILVFDLGGGTFDVSLLTID----------NG 241

  Fly   246 VVQVLGVGYDRTLGGLEIQLRLRDYLAQEFNALKKTKTDVTTSPRALAKLFKEAGRLKNVLSANT 310
            |.:|:....|..|||.:...|:.::..:.:.  |||..||....||:.||.:|..:.|..||:..
Mouse   242 VFEVVATNGDTHLGGEDFDQRVMEHFIKLYK--KKTGKDVRKDNRAVQKLRREVEKAKRALSSQH 304

  Fly   311 EFFAQIENLIEDIDFKLPVTREKLEQLCEDLWPRATKPLEEALASSHLSLDVINQVILFGGGTRV 375
            :...:||:..|..||...:||.|.|:|..||:....||:::.|..|.|....|::::|.||.||:
Mouse   305 QARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRI 369

  Fly   376 PRVQETIKAVIK-QELGKNLNADESATMGAVYKAADLSAGFKVKKFVVKDATLFPLQVSFERDPG 439
            |::|:.:|.... :|..:.:|.||:...||..:|..||........|:.|  :.||.:..|
Mouse   370 PKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLD--VCPLTLGIE---- 428

  Fly   440 DGAAVKQVKRALFALMNPYPQKKVITF-----NKHTDDFEFYVNYADLDRYSKEEIAALGSLNVT 499
               .|..|...|.......|.||...|     |:.|...:.|    :.:|...::...||:.::|
Mouse   429 ---TVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVY----EGERPLTKDNHLLGTFDLT 486

  Fly   500 KVQLKQVKELLEKSKKELVDNKG---IKAYFYLDDSGIFRCTGVEYVYEKQKPEDDADEDSTLSK 561
            .:....               :|   |:..|.:|.:||.|.|              |::..|.:|
Mouse   487 GIPPAP---------------RGVPQIEVTFEIDVNGILRVT--------------AEDKGTGNK 522

  Fly   562 FGSTLSKLFTKEGEEKKDNSEQEEAANAGEEPSKSEDNEKAKEEDASKEQKSEESTKQDTEAKNE 626
                 :|:.....:.:....|.|...|         |.||..|||        :..|:..:.:||
Mouse   523 -----NKITITNDQNRLTPEEIERMVN---------DAEKFAEED--------KKLKERIDTRNE 565

  Fly   627 TIKLVTVKSPVTYESQTQFVVPLVGSAYDQSVAKLAAINKAEEQRVRLESAFNALEAHIIEVQQK 691
                                                                  ||::...::.:
Mouse   566 ------------------------------------------------------LESYAYSLKNQ 576

  Fly   692 L-DEESYAKCATAEEKEKLLAECSTLGEWLYE----DLEDPKAEIYE-EKLAQ--LKKL 742
            : |:|......::|:||.:........|||..    |:||.||:..| |::.|  :.||
Mouse   577 IGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKL 635

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG2918	NP_001162645.1	HSP70	22..625	CDD:278441	165/618 (27%)
		HYOU1-like_NBD	23..409	CDD:212672	120/393 (31%)
		TAF4	<572..>627	CDD:296797	11/54 (20%)
Hspa5	NP_001156906.1	Required for interaction with KIAA1324. /evidence=ECO:0000250\|UniProtKB:P11021	1..81		26/79 (33%)
		HSP70	31..636	CDD:365808	187/745 (25%)
		Nucleotide-binding (NBD). /evidence=ECO:0000250\|UniProtKB:P11021	126..281		50/171 (29%)
		Interdomain linker. /evidence=ECO:0000250\|UniProtKB:G3I8R9	410..420		2/11 (18%)
		Substrate-binding (SBD). /evidence=ECO:0000250\|UniProtKB:P11021	421..501		19/105 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	632..655		2/4 (50%)
		Prevents secretion from ER	652..655
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810