Protein Alignment Sik2 and HSL1

DIOPT Version :10

• Sequence 1: NP_569972.1
• Gene: Sik2 / 31170
• FlyBaseID: FBgn0025625
• Length: 1398
• Species: Drosophila melanogaster
• Sequence 2: NP_012821.2
• Gene: HSL1 / 853760
• SGDID: S000001584
• Length: 1518
• Species: Saccharomyces cerevisiae
• Alignment Length: 1069
• Identity: 262/1069 - (24%)
• Similarity: 410/1069 - (38%)
• Gaps: 297/1069 - (27%)

  Fly    99 QKLFASGGGSTPGPSPTSSAVGAGGISGKDLLKLKEPMRVGFYDIERTIGKGNFAVVKLARHRIT 163
            :::..|...:|...|...|.|.....|.    |.|....||.:.:.:|:|||:...|:||::..|
Yeast    43 ERVVQSVNDATKRLSQPDSTVSVATKSS----KRKSRDTVGPWKLGKTLGKGSSGRVRLAKNMET 103

  Fly   164 KNEVAIKIIDK------------------------------------SQLDQTNLQKVYREVEIM 192
            ....||||:.|                                    |...|||...:.||:.||
Yeast   104 GQLAAIKIVPKKKAFVHCSNNGTVPNSYSSSMVTSNVSSPSIASREHSNHSQTNPYGIEREIVIM 168

  Fly   193 KRLKHPHIIKLYQVMETKNMIYIVSEYASQGEIFDYIAKYGRMSESAARFKFWQIISAVEYCHKK 257
            |.:.|.:::.|::|.|.|:.:|:|.||...||:|||:...|::.|..|...|.||:..|.|||..
Yeast   169 KLISHTNVMALFEVWENKSELYLVLEYVDGGELFDYLVSKGKLPEREAIHYFKQIVEGVSYCHSF 233

  Fly   258 GIVHRDLKAENLLLD-LNMNIKIADFGFSNHFKPGELLATWCGSPPYAAPEVFEGKQYTGPEIDI 321
            .|.|||||.|||||| .|..|||||||.:....|.:||.|.||||.||:||:..|:.|.|...|:
Yeast   234 NICHRDLKPENLLLDKKNRRIKIADFGMAALELPNKLLKTSCGSPHYASPEIVMGRPYHGGPSDV 298

  Fly   322 WSLGVVLYVLVCGALPFDGSTLQSLRDRVLSGRFRIPFFMSSECEHLIRRMLVLEPTRRYTIDQI 386
            ||.|:||:.|:.|.|||:...::.|..:|.||::::|..:|||...||.::||::|.:|.|..
Yeast   299 WSCGIVLFALLTGHLPFNDDNIKKLLLKVQSGKYQMPSNLSSEARDLISKILVIDPEKRITTQ-- 361

  Fly   387 KRHRWMCPELLEHVLIAKY---------------NLGAERQTS-------VEPS----------- 418
                    |:|:|.||.||               |:...:..|       |.||
Yeast   362 --------EILKHPLIKKYDDLPVNKVLRKMRKDNMARGKSNSDLHLLNNVSPSIVTLHSKGEID 418

  Fly   419 EDILR-IMAEYVGIGSDKTRASLKKNTYDHVAAIYLLLQDRVSHKKEQSNGLGA------SALAS 476
            |.||| :...:.|:..:...|.|.:.........|.||   :.:|:..|..|.:      ||..|
Yeast   419 ESILRSLQILWHGVSRELITAKLLQKPMSEEKLFYSLL---LQYKQRHSISLSSSSENKKSATES 480

  Fly   477 STSASRMIYSSRNDHQPTQQQSQQQSKTISTSSILAKDQCHKRLSRHQTVLMSERNAHAGATPTV 541
            |.:..|:.|:|:..:....:......||:.:..|.::|          |..:::.||..|....:
Yeast   481 SVNEPRIEYASKTANNTGLRSENNDVKTLHSLEIHSED----------TSTVNQNNAITGVNTEI 535

  Fly   542 PDPGPGYYAKYGPLQLPLPLTGHSHLTGYLNGGGVEVDASGIPLPMRYTPLPTAASPAPSNCSST 606
            ..|.....:::....|..|.:.             :.:|..:.||           ||....:::
Yeast   536 NAPVLAQKSQFSINTLSQPESD-------------KAEAEAVTLP-----------PAIPIFNAS 576

  Fly   607 SSRVGRHSLSSSSPRSHRPVAISLSIDNNPSLANLRCREMMEAGGGPV--------------GAV 657
            |||:.|:|.:|.|.||.|.:.:|   ::..||:....||.:.....|:              .|:
Yeast   577 SSRIFRNSYTSISSRSRRSLRLS---NSRLSLSASTSRETVHDNEMPLPQLPKSPSRYSLSRRAI 638

  Fly   658 GVPLASKQLHQTISEFIIKQSTEDCRALLQQST---------AVAEGK---------DDPPKAES 704
            ....::|.:|:::|...| .:|...|..||.|.         ::::..         |||..::.
Yeast   639 HASPSTKSIHKSLSRKNI-AATVAARRTLQNSASKRSLYSLQSISKRSLNLNDLLVFDDPLPSKK 702

  Fly   705 SVGGVPPPASTTPTSSTAGPESGS---APCPGEINGKTI----------------------KTMS 744
                  |.:.....|.....||.|   ..|...:.|..:                      .|.|
Yeast   703 ------PASENVNKSEPHSLESDSDFEILCDQILFGNALDRILEEEEDNEKERDTQRQRQNDTKS 761

  Fly   745 SSSSF------DSKANLGQSF-------RYKMSAEASK---------LFQ---TLQESPLPVEQR 784
            |:.:|      .:|.|.|..:       ::.||.:.|:         :|:   ||..|.|. ||:
Yeast   762 SADTFTISGVSTNKENEGPEYPTKIEKNQFNMSYKPSENMSGLSSFPIFEKENTLSSSYLE-EQK 825

  Fly   785 TKRRVHVGSTNGSGGDSGQETNDAKSNGDSRSEKKVLAQGSSSTDEGCETDQGNDPGS-----AS 844
            .||......||.....:.||        ..|.|||:..:         :..:.||..|     ..
Yeast   826 PKRAALSDITNSFNKMNKQE--------GMRIEKKIQRE---------QLQKKNDRPSPLKPIQH 873

  Fly   845 QESKGS---NGGGSGNANGGPTSHSSSDLTRLVGTTTSGQSHK---------------------- 884
            ||.:.:   |..|..:.:..|..:.|..:...|.:...|...|
Yeast   874 QELRVNSLPNDQGKPSLSLDPRRNISQPVNSKVESLLQGLKFKKEPASHWTHERGSLFMSEHVED 938

  Fly   885 -----------------MRSYASSSSSSGVLAGSAGSYSKSLSQNLSRGSSKSNCSGPYDSLDFA 932
                             :.:.|:||....|||.|: :..|.:   ||..||..|.|..:.:|...
Yeast   939 EKPVKASDVSIESSYVPLTTVATSSRDPSVLAESS-TIQKPM---LSLPSSFLNTSMTFKNLSQI 999

  Fly   933 LPSGKGSLPSCMGSSSMLATPTPASASPA 961
            |...        |....|:.|...|.|.|
Yeast  1000 LADD--------GDDKHLSVPQNQSRSVA 1020

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sik2	NP_569972.1	STKc_SIK	140..392	CDD:270973	107/288 (37%)
		UBA_SIK	418..458	CDD:270523	11/51 (22%)
HSL1	NP_012821.2	STKc_BRSK1_2	79..369	CDD:270983	111/299 (37%)
		AMPKA_C_like	1347..1517	CDD:473060