Protein Alignment Actn and MICALL2

DIOPT Version :10

• Sequence 1: NP_477485.1
• Gene: Actn / 31166
• FlyBaseID: FBgn0000667
• Length: 917
• Species: Drosophila melanogaster
• Sequence 2: NP_891554.1
• Gene: MICALL2 / 79778
• HGNCID: 29672
• Length: 904
• Species: Homo sapiens
• Alignment Length: 223
• Identity: 72/223 - (32%)
• Similarity: 105/223 - (47%)
• Gaps: 36/223 - (16%)

  Fly   147 MTAKEGLLLWCQRKTAPYKNVNVQNFHLSFKDGLAFCALIHRHRPDLIDYAKLSKDNPLENLNTA 211
            |.|...|..||:::...|::||:.|...||:|||||||::||||||||:::.|.|:|..||...|
Human     1 MAAIRALQQWCRQQCEGYRDVNICNMTTSFRDGLAFCAILHRHRPDLINFSALKKENIYENNKLA 65

  Fly   212 FDVAEKYLDIPRMLDPDDLINTPKPDERAIMTYVSCYYHAFQGAQQVGNVTHVPEPTRQYTYVPN 276
            |.|||::|.||.:||.:|::....||..:|:||||.||:.|.|...:|.:..|...:......|:
Human    66 FRVAEEHLGIPALLDAEDMVALKVPDRLSILTYVSQYYNYFHGRSPIGGMAGVKRASEDSEEEPS 130

  Fly   277 NYNAETAANRICK-------VLKVNQENERLMEEYERLASDLLEWIRRTMPWLNSRQADNSLAG- 333
            ...|...|.::..       .|...|.|..:....|.....             ..:.|.:|||
Human   131 GKKAPVQAAKLPSPAPARKPPLSPAQTNPVVQRRNEGAGGP-------------PPKTDQALAGS 182

  Fly   334 ---------------VQKKLEEYRTYRR 346
                           ||:.|.:.|.|.|
Human   183 LVSSTCGVCGKHVHLVQRHLADGRLYHR 210

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Actn	NP_477485.1	CH_ACTN_rpt1	31..135	CDD:409063
		CH_ACTN_rpt2	139..252	CDD:409065	51/104 (49%)
		SPEC	302..525	CDD:238103	11/61 (18%)
		SPEC	420..647	CDD:238103
		SPEC	538..760	CDD:238103
		EFh	775..842	CDD:238008
		EFhand_Ca_insen	847..913	CDD:430177
MICALL2	NP_891554.1	Forms an intramolecular interaction with the C-terminal coiled coil domain keeping the protein in a closed conformation. /evidence=ECO:0000250	1..260		72/223 (32%)
		CH_MICALL2	2..107	CDD:409102	50/104 (48%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	117..178		9/73 (12%)
		LIM_Mical_like_1	188..241	CDD:188828	6/23 (26%)
		PHA03247	<243..649	CDD:223021
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	251..722
		Mediates targeting to the cell plasma membrane. /evidence=ECO:0000250	261..697
		Necessary and sufficient for interaction with actinins. /evidence=ECO:0000250	261..388
		Forms an intramolecular interaction with the N-terminal Calponin-homology and LIM zinc-binding domains-containing region keeping the protein in a closed conformation. /evidence=ECO:0000250	698..807
		bMERB_dom	743..872	CDD:463467
		Mediates interaction with RAB13 and is required for transition from the closed to the opened conformation. /evidence=ECO:0000250	807..903