DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Actn and Sptan1

DIOPT Version :9

Sequence 1:	NP_001259165.1	Gene:	Actn / 31166	FlyBaseID:	FBgn0000667	Length:	917	Species:	Drosophila melanogaster
Sequence 2:	XP_008759895.1	Gene:	Sptan1 / 64159	RGDID:	621714	Length:	2498	Species:	Rattus norvegicus

Alignment Length:	952	Identity:	251/952 - (26%)
Similarity:	440/952 - (46%)	Gaps:	157/952 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    20 EEWEREGLLDPAWEKQQKKTFTAWCNSHLRKAGTAIDNIEEDFRNGLK--LMLLLEVISGETLPK 82
            ::|  :.|:..:.||.||          |::|     |.:::|..|:|  ...|.||.:  .|..
  Rat  1643 DQW--QFLVQKSAEKSQK----------LKEA-----NKQQNFNTGIKDFDFWLSEVEA--LLAS 1688

  Fly    83 PDRGKMRFHKIANVNKALDFIASKGVHLV--SIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVE 145
            .|.||    .:|:||..|     |...|:  .|.|.|   ..||.......:::...|.....|:
  Rat  1689 EDYGK----DLASVNNLL-----KKHQLLEADISAHE---DRLKDLNSQADSLMTSSAFDTSQVK 1741

  Fly   146 EMTAKEGLLLWCQRKTAPYKNVNVQNFHLSFKDGLAFCALIHRHRPDLIDYAKLSKDNPLENLNT 210
            |           :|.|...:...:::...|.:                   ||||:.:.|.....
  Rat  1742 E-----------KRDTINGRFQKIKSMATSRR-------------------AKLSESHRLHQFFR 1776

  Fly   211 AFDVAEKYLDIPRML--------DPDDLINTPKPDERAIMTYVSCYYHAFQGAQQVGNVTHVPEP 267
            ..|..|.::...::|        |...:.|..|..:| :...::.:..|.||....|
  Rat  1777 DMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKR-LEAELAAHEPAIQGVLDTG-------- 1832

  Fly   268 TRQYTYVPNNYNAETAANRICKVLKVNQE--------NERLME--EYERLASDLLE---WIRRTM 319
              :.....|....|....|:.:.::..:|        .:||.|  ||::..:::.|   ||...|
  Rat  1833 --KKLSDDNTIGQEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKM 1895

  Fly   320 PWLNSRQADNSLAGVQKKLEEYRTYRRKHKPPRVEQKAKLETNFNTLQTKLR--LSNRPAYLPTE 382
            ..:.|....::||.:|..|:::..:               ||:|...:.::.  .:|....:...
  Rat  1896 TLVASEDYGDTLAAIQGLLKKHEAF---------------ETDFTVHKDRVNDVCTNGQDLIKKN 1945

  Fly   383 GKTVSDISNSWKGLELAEKAFEEWLLAETMRLERLEHLAQ--KFKHKADAHEDWTRGKEEMLQSQ 445
            .....:||:..|||.......|:   |...|..:|:..:.  :|..|||..|.|...||..|::.
  Rat  1946 NHHEENISSKMKGLNGKVSDLEK---AAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTD 2007

  Fly   446 DFRQCKLNELKALKKKHEAFESDLAA-HQDRVEQIAAIAQELNTLEYHDCVSVNARCQRICDQWD 509
            |:.: .|:.::.|..|.|.|::.|.| .|:.:..|.|:..:|...::....::.||...:..:|.
  Rat  2008 DYGR-DLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHIQSKAIEARHASLMKRWT 2071

  Fly   510 RLGALTQRRRTALDEAERILEKIDILHLEFAKRAAPFNNWLDGTREDLVDMFIVHTMEEIQGLIQ 574
            :|.|.:..|:..|.||:....|::.|.|.|||:|:.||:|.:...|||.|....:::|||:.|.:
  Rat  2072 QLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALRE 2136

  Fly   575 AHDQFKATLGEADKEFNLIVNLVREVESIVKQHQIPGGLENPYTTLTANDMTRKWSDVRQLVPQR 639
            |||.|:::|..|..:||.:..|.|:::|.       ....||||..|...:...|.::::::.:|
  Rat  2137 AHDAFRSSLSSAQADFNQLAELDRQIKSF-------RVASNPYTWFTMEALEETWRNLQKIIKER 2194

  Fly   640 DQTLANELRKQQNNEMLRRQFAEKANIVGPWIERQ----MDAVT----------AIGMGL----- 685
            :..|..|.|:|:.|:.||::||:.||....||:..    :|.:.          .:|..|
  Rat  2195 ELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSC 2259

  Fly   686 ----QGSLEDQLHRLKEYEQAVYAYKPNIEELEKIHQAVQESMIFENRYTNYTMETLRVGWEQLL 746
                .|:||.||...|...|.:.|.:..::::|.:..|::|::|.:|:||.::...|...|:||.
  Rat  2260 MVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLD 2324

  Fly   747 TSINRNINEVENQILTRDSKGISQEQLNEFRSSFNHFDKNRTGRLSPEEFKSCLVSLGYSIGKDR 811
            ....|..:.:|.||..|::.|:::|.|.||...|.||||:::|||:.:||||||.||||.:....
  Rat  2325 QLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVE 2389

  Fly   812 QG--DLDFQRILAVVDPNNTGYVHFDAFLDFM-TRESTDTDTAEQVIDSFRILAAD-KPYILPDE 872
            :|  |.:|:.||..||||..|:|....::.|| :||:.:..::|::..:||.|::: |||:..:|
  Rat  2390 EGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEE 2454

  Fly   873 LRRELPPDQAEYCIQRMPPYKGPNG--VPGALDYMSFSTALY 912
            |.:.|..:||:||:..|.||....|  :|.|.||:.|:.:|:
  Rat  2455 LYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLF 2496

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Actn	NP_001259165.1	CH_ACTN_rpt1	31..135	CDD:409063	28/107 (26%)
		CH_ACTN_rpt2	139..252	CDD:409065	17/120 (14%)
		SPEC	302..525	CDD:238103	53/232 (23%)
		SPEC	420..647	CDD:238103	67/229 (29%)
		SPEC	538..760	CDD:238103	70/244 (29%)
		EFh	775..842	CDD:238008	33/69 (48%)
		EFhand_Ca_insen	847..913	CDD:400872	24/69 (35%)
Sptan1	XP_008759895.1	Spectrin	44..147	CDD:278843
		SPEC	150..361	CDD:238103
		SPEC	362..572	CDD:238103
		SPEC	468..678	CDD:238103
		SPEC	679..890	CDD:238103
		Spectrin	890..>958	CDD:278843
		SH3_Alpha_Spectrin	971..1023	CDD:212742
		SPEC	1092..1339	CDD:238103
		SPEC	1234..1445	CDD:238103
		SPEC	1446..1663	CDD:238103	8/36 (22%)
		Spectrin	1663..1767	CDD:278843	31/147 (21%)
		SPEC	1770..1979	CDD:238103	43/237 (18%)
		SPEC	1876..2087	CDD:238103	52/229 (23%)
		SPEC	1983..2201	CDD:238103	67/225 (30%)
		SPEC	2101..2338	CDD:238103	70/243 (29%)
		EFh	2353..2421	CDD:238008	31/67 (46%)
		EF-hand_7	2356..2421	CDD:290234	29/64 (45%)
		EFhand_Ca_insen	2428..2496	CDD:285885	23/67 (34%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D543832at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.920