DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Actn and actn2b

DIOPT Version :9

Sequence 1:	NP_001259165.1	Gene:	Actn / 31166	FlyBaseID:	FBgn0000667	Length:	917	Species:	Drosophila melanogaster
Sequence 2:	NP_001032662.1	Gene:	actn2b / 641575	ZFINID:	ZDB-GENE-051127-39	Length:	895	Species:	Danio rerio

Alignment Length:	922	Identity:	620/922 - (67%)
Similarity:	751/922 - (81%)	Gaps:	32/922 - (3%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MMMENGLSMEYGDG-----YMEQEEEWEREGLLDPAWEKQQKKTFTAWCNSHLRKAGTAIDNIEE 60
            ||.:..||:.|.:|     ||.||:||:|:.||||||||||:|||||||||.||||||.|:||||
Zfish     1 MMNQIELSVPYDNGYEIDEYMMQEDEWDRDLLLDPAWEKQQRKTFTAWCNSRLRKAGTQIENIEE 65

  Fly    61 DFRNGLKLMLLLEVISGETLPKPDRGKMRFHKIANVNKALDFIASKGVHLVSIGAEEIVDGNLKM 125
            ||||||||||||||||||.||||||||||||||||||||||||.||||.|||||||||||||:||
Zfish    66 DFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDFITSKGVKLVSIGAEEIVDGNVKM 130

  Fly   126 TLGMIWTIILRFAIQDISVEEMTAKEGLLLWCQRKTAPYKNVNVQNFHLSFKDGLAFCALIHRHR 190
            |||||||||||||||||||||.:||||||||||||||||:||||||||:|:|||||.||||||||
Zfish   131 TLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHVSWKDGLALCALIHRHR 195

  Fly   191 PDLIDYAKLSKDNPLENLNTAFDVAEKYLDIPRMLDPDDLINTPKPDERAIMTYVSCYYHAFQGA 255
            |||||||||:||:||.|||.|||:|||:||||:|||.:|:::|||||||||||||||:||||.||
Zfish   196 PDLIDYAKLNKDDPLGNLNLAFDIAEKHLDIPKMLDAEDILSTPKPDERAIMTYVSCFYHAFAGA 260

  Fly   256 QQVGNVTHVPEPTRQYTYVPNNYNAETAANRICKVLKVNQENERLMEEYERLASDLLEWIRRTMP 320
            :|                      ||||||||||||.||||||:|||:||||||:||||||||:|
Zfish   261 EQ----------------------AETAANRICKVLGVNQENEKLMEDYERLASELLEWIRRTIP 303

  Fly   321 WLNSRQADNSLAGVQKKLEEYRTYRRKHKPPRVEQKAKLETNFNTLQTKLRLSNRPAYLPTEGKT 385
            ||.:|.|:.::..:|:|||::|.|||.||||:|::|.:||.:|||||||||:|||||::|:|||.
Zfish   304 WLENRAAEKTMVEMQRKLEDFRDYRRMHKPPKVQEKCQLEISFNTLQTKLRISNRPAFMPSEGKM 368

  Fly   386 VSDISNSWKGLELAEKAFEEWLLAETMRLERLEHLAQKFKHKADAHEDWTRGKEEMLQSQDFRQC 450
            ||||:::|:|||.|||.:|||||.|..||||:||||:||:.||..||.|..||||||..:|:...
Zfish   369 VSDIASAWQGLEQAEKGYEEWLLTEIRRLERVEHLAEKFRQKASNHESWATGKEEMLTRKDYESA 433

  Fly   451 KLNELKALKKKHEAFESDLAAHQDRVEQIAAIAQELNTLEYHDCVSVNARCQRICDQWDRLGALT 515
            .|.|::||.:|||||||||:|||||||||||||||||.|:|||..|||.|||.|||.||.||.||
Zfish   434 SLMEVRALLRKHEAFESDLSAHQDRVEQIAAIAQELNELDYHDVASVNQRCQSICDLWDHLGTLT 498

  Fly   516 QRRRTALDEAERILEKIDILHLEFAKRAAPFNNWLDGTREDLVDMFIVHTMEEIQGLIQAHDQFK 580
            ||||.||:..|::||.:|.|.||:|||:||||||::|..|||.|||||||:||:|.||.||:|||
Zfish   499 QRRREALERTEKLLETVDQLFLEYAKRSAPFNNWMEGAMEDLQDMFIVHTIEEVQTLIAAHEQFK 563

  Fly   581 ATLGEADKEFNLIVNLVREVESIVKQHQIPGGLENPYTTLTANDMTRKWSDVRQLVPQRDQTLAN 645
            |||.|||.|...|:.:.:||..|.:.:.|.|.|.|||:|:||.::..||..|::||||||..|..
Zfish   564 ATLSEADAERQAILGIQQEVLKIFQSYGIRGDLTNPYSTITAEEIAIKWDKVKKLVPQRDSALQE 628

  Fly   646 ELRKQQNNEMLRRQFAEKANIVGPWIERQMDAVTAIGMGLQGSLEDQLHRLKEYEQAVYAYKPNI 710
            ||.:|..||.||||||.:||::||||:.:|:.:....|.:.|:||||:.:||:||..:.:||.||
Zfish   629 ELARQHANERLRRQFAAQANLIGPWIQTRMEEIGHCSMAMGGTLEDQMTQLKQYEHVIVSYKSNI 693

  Fly   711 EELEKIHQAVQESMIFENRYTNYTMETLRVGWEQLLTSINRNINEVENQILTRDSKGISQEQLNE 775
            :.||..||.:|||:||:|::||||||.:|||||.|||:|.|.|||:|.||||||:|||||||::|
Zfish   694 DRLEGDHQLIQESLIFDNKHTNYTMEHVRVGWELLLTTIARTINEIETQILTRDAKGISQEQMHE 758

  Fly   776 FRSSFNHFDKNRTGRLSPEEFKSCLVSLGYSIGKDRQGDLDFQRILAVVDPNNTGYVHFDAFLDF 840
            |||||||||:...|.:..::|::||:|:||.:     |:::|.||:.:||||.:|.|.|.:|:||
Zfish   759 FRSSFNHFDRKENGGMDSDDFRACLISMGYDL-----GEVEFARIMMLVDPNASGNVSFQSFIDF 818

  Fly   841 MTRESTDTDTAEQVIDSFRILAADKPYILPDELRRELPPDQAEYCIQRMPPYKGPNGVPGALDYM 905
            ||||:.|||||||||.||:||||||||||.||||||||||||||||.|||||.||..:||||||.
Zfish   819 MTRETADTDTAEQVIASFKILAADKPYILSDELRRELPPDQAEYCISRMPPYCGPGALPGALDYT 883

  Fly   906 SFSTALYGETDL 917
            :||||||||:||
Zfish   884 AFSTALYGESDL 895

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Actn	NP_001259165.1	CH_ACTN_rpt1	31..135	CDD:409063	95/103 (92%)
		CH_ACTN_rpt2	139..252	CDD:409065	93/112 (83%)
		SPEC	302..525	CDD:238103	148/222 (67%)
		SPEC	420..647	CDD:238103	138/226 (61%)
		SPEC	538..760	CDD:238103	123/221 (56%)
		EFh	775..842	CDD:238008	31/66 (47%)
		EFhand_Ca_insen	847..913	CDD:400872	55/65 (85%)
actn2b	NP_001032662.1	CH	43..141	CDD:214479	90/97 (93%)
		CH	153..258	CDD:237981	86/104 (83%)
		Spectrin	282..391	CDD:278843	70/108 (65%)
		SPEC	403..630	CDD:238103	138/226 (61%)
		Spectrin	638..740	CDD:278843	56/101 (55%)
		EFh	758..820	CDD:238008	31/66 (47%)
		EFhand_Ca_insen	825..891	CDD:285885	55/65 (85%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	198	1.000	Domainoid score	I3028
eggNOG	1	0.900	-	-		E2759_KOG0035
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1295	1.000	Inparanoid score	I156
OMA	1	1.010	-	-		QHG54323
OrthoDB	1	1.010	-	-		D543832at2759
OrthoFinder	1	1.000	-	-		FOG0000815
OrthoInspector	1	1.000	-	-		otm24529
orthoMCL	1	0.900	-	-		OOG6_101135
Panther	1	1.100	-	-	O	PTHR11915
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X512
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	13	12.880