Protein Alignment Actn and sptbn2

DIOPT Version :9

• Sequence 1: NP_001259165.1
• Gene: Actn / 31166
• FlyBaseID: FBgn0000667
• Length: 917
• Species: Drosophila melanogaster
• Sequence 2: XP_012817859.1
• Gene: sptbn2 / 100486199
• XenbaseID: XB-GENE-1008690
• Length: 2437
• Species: Xenopus tropicalis
• Alignment Length: 1000
• Identity: 312/1000 - (31%)
• Similarity: 472/1000 - (47%)
• Gaps: 234/1000 - (23%)

  Fly    13 DGYMEQEEEWEREGLLDPAWEKQQKKTFTAWCNSHLRKAGTAIDNIEEDFRNGLKLMLLLEVISG 77
            :|..:|.:: |||.:        ||||||.|.||||.:....|.::..|.|:|..|:.||||:||
 Frog    81 EGRFKQLQD-EREAV--------QKKTFTKWVNSHLARVTCRISDLYNDLRDGRMLIRLLEVLSG 136

  Fly    78 ETLPKPDRGKMRFHKIANVNKALDFIASKGVHLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDI 142
            |.||||.:|:||.|.:.||:|||.|:..:.|||.::|:.:|||||.::|||:||||||||.||||
 Frog   137 EQLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDI 201

  Fly   143 SV------EEMTAKEGLLLWCQRKTAPYKNVNVQNFHLSFKDGLAFCALIHRHRPDLIDYAKLSK 201
            ||      |:.:||:.||||||.|||.|.||||.||..|::|||||.|::|:||||:||:..|.|
 Frog   202 SVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDIIDFDTLKK 266

  Fly   202 DNPLENLNTAFDVAEKYLDIPRMLDPDDLINTPKPDERAIMTYVSCYYHAFQGAQQVGNVTHVPE 266
            .|...||..||:|||:.|.:.::|||:| :|..:|||::|:|||:.:||.|...:.:.
 Frog   267 SNAHHNLQNAFNVAERELGLTKLLDPED-VNVDQPDEKSIITYVATFYHYFSKMKALA------- 323

  Fly   267 PTRQYTYVPNNYNAETAANRICKVLKVNQENERLMEEYERLASDLLEWIRRTMPWLNSRQADNSL 331
                           ....||.|||....|.::|:|.||.|||:||:||.:.:..||.|:..|||
 Frog   324 ---------------VEGKRIGKVLDQAIEADQLIENYEGLASELLQWIEQMIITLNDRKLANSL 373

  Fly   332 AGVQKKLEEYRTYRRKHKPPRVEQKAKLETNFNTLQTKLRLSNRPAYLPTEGKTVSDISNSWKGL 396
            :|||.:|:.:.|||...|||:..:|..||....|:|:|:|.:|:..|.|.|||.:|||:.:|:.|
 Frog   374 SGVQNQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYTPREGKLISDINKAWERL 438

  Fly   397 ELAEKAFEEWLLAETMRLERLEHLAQKFKHKADAHEDWTRGKEEMLQSQDFRQCKLNELKALKKK 461
            |.||...|..|..|.:|.|:||.||.:|..||...|.|. .:.:.|.:||.....|..::|..:|
 Frog   439 EKAEHERELALRNELIRQEKLEQLAARFDRKAAMRETWL-SENQRLVAQDNFGVDLTAVEAALRK 502

  Fly   462 HEAFESDLAAHQDRVEQIAAIAQELNTLEYHDCVSVNARCQRICDQWDRLGALTQRRRTALD--- 523
            |||.|:|:.|:.:||:.:.|:|.||....|||...:.||...:....|.|..|...||..|.
 Frog   503 HEAIETDILAYSERVQAVNAVADELEAENYHDVRRILARKCNVIRLSDYLRELVTARRQRLHMNL 567

  Fly   524 EAERILEKIDIL-----HLEFAKRAAPFNNWLDGTREDLVDMFIVHTMEEIQGLIQAHDQFKATL 583
            |.:|:.:.:..|     .::...::..:...|.|. |||:.   .||:.|....:|| ::.|:..
 Frog   568 ELQRMFQDLHYLMDWTEDMKGRLQSEDYGKHLHGV-EDLLQ---THTLVEADIAVQA-ERVKSVT 627

  Fly   584 GEADK-----------EFNLIVNLVREVE---------SIVKQHQIPGG---------------- 612
            ..|.|           :..|:.|.|..:|         |..::.::...
 Frog   628 AVAQKFMTEDEGYKPCDPELVRNRVHLLEQRYAELCNLSAARRSKLEESRRLWKFFWDMGEEEAW 692

  Fly   613 --------LENPY-TTLTAN------------DMTRKWSDVRQLVPQRDQTL------ANELR-- 648
                    |.:.| ..||::            :|:.....::|.|.:.::.:      |.|:.
 Frog   693 IREQSRLLLSDDYGKDLTSSLRLISKHNAFRGEMSGHRGPLQQTVAEGEELISKGHFGAGEIEER 757

  Fly   649 ----KQQNNEM----------LRR-----QFAEKANIVGPWIERQMDAVTAIGMGLQG----SLE 690
                |.|..||          ||.     ||...||....||   :|.:..:.....|    |.:
 Frog   758 IKEVKGQWTEMEALAEDRERRLREAYSYYQFQADANDAEAWI---LDTLRLVSTSEVGHDEYSTQ 819

  Fly   691 DQLHRLKEYEQAVYAYKPNIEELEK---------------------------------IH--QAV 720
            ..:.:.||.|:.:.:::|.|:.|.:                                 .|  ||:
 Frog   820 TLVKKHKEIEEEILSHRPAIDALHEQARGLPPAYSHSPEVDGRLPALEQRYEELVALAAHRKQAL 884

  Fly   721 QESMIF----------------ENRYTNYT--------METLRVGWEQLLTSINR------NINE 755
            |:|:..                :.::.|.|        :|.::..:|.|...:|.      ::||
 Frog   885 QDSLALHKMFSEADACLLWIDEKEQWLNVTEIPEKLEDLEVVQQRFETLEAEMNNLASRIASVNE 949

  Fly   756 VENQILTRDSKGIS-----QEQL----NEFRSSFN---------------HFDKNRTGRLSPEEF 796
            |..|:||.|.:..:     |:||    ||||...:               |.:.|.|.....|:.
 Frog   950 VAGQLLTTDQRNENSIRDMQDQLNSRWNEFRKLVDQKKGALDSALDIQNYHLECNETVCWMREKT 1014

  Fly   797 KSCLVSLGYSIGKDRQGDLDFQRIL 821
            |  ::....|:|.|..|....||.|
 Frog  1015 K--VIESTQSLGNDLAGVTALQRKL 1037

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Actn	NP_001259165.1	CH_ACTN_rpt1	31..135	CDD:409063	53/103 (51%)
		CH_ACTN_rpt2	139..252	CDD:409065	64/118 (54%)
		SPEC	302..525	CDD:238103	90/225 (40%)
		SPEC	420..647	CDD:238103	65/297 (22%)
		SPEC	538..760	CDD:238103	62/374 (17%)
		EFh	775..842	CDD:238008	14/61 (23%)
		EFhand_Ca_insen	847..913	CDD:400872
sptbn2	XP_012817859.1	CH	94..197	CDD:237981	56/110 (51%)
		CH	213..316	CDD:237981	57/103 (55%)
		Spectrin	341..451	CDD:278843	50/109 (46%)
		SPEC	465..563	CDD:197544	33/98 (34%)
		SPEC	568..783	CDD:238103	36/219 (16%)
		SPEC	681..888	CDD:238103	34/209 (16%)
		SPEC	785..994	CDD:238103	40/211 (19%)
		SPEC	997..1206	CDD:238103	11/42 (26%)
		SPEC	1210..1419	CDD:238103
		SPEC	1420..1630	CDD:238103
		SPEC	1527..1734	CDD:238103
		SPEC	1632..1843	CDD:238103
		SPEC	1844..2051	CDD:238103
		SPEC	2060..>2117	CDD:197544
		PH	2272..2378	CDD:278594
		PH_beta_spectrin	2272..2377	CDD:269975

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D543832at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.920