DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Rbf and Rbl1

DIOPT Version :9

Sequence 1:	NP_525036.2	Gene:	Rbf / 31027	FlyBaseID:	FBgn0015799	Length:	845	Species:	Drosophila melanogaster
Sequence 2:	NP_035379.2	Gene:	Rbl1 / 19650	MGIID:	103300	Length:	1063	Species:	Mus musculus

Alignment Length:	1106	Identity:	313/1106 - (28%)
Similarity:	480/1106 - (43%)	Gaps:	335/1106 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSEPDPQELGAEVVSGLVATSDDRLEMINAEYTNLCRDLNMDRQTELQGYETYLEVSQRCSMEGT 65
            |.|.:|...||    ..||.:.:.|:.       ||::||:|..:..:..:.:..:....|:||.
Mouse     1 MFEDEPHAEGA----AAVAAAREALQA-------LCQELNLDEGSAAEALDDFTAIRGNYSLEGE 54

  Fly    66 ASHWMCCAIYTACRRTSTPTVTGQNAVVKGNCVSLNNLLRCCKMSIYEFKTKIKQWCDMANLPQE 130
            ..||:.|::|.|||::..|||  ...|::||||||..:||..|:|:.:|.:|:|:|.||:|||||
Mouse    55 VIHWLACSLYVACRKSIIPTV--GKGVMEGNCVSLTRILRSAKLSLIQFFSKMKKWMDMSNLPQE 117

  Fly   131 FVNEIEDLDRKFSITFMLHKRFRIIMDMIFSCP-------PNEKKHSKYISLHGNHAHGKCSYIK 188
            |...||.|:|.|.::.::.|:|..|...||..|       |..:|..:.          .||...
Mouse   118 FRERIERLERNFEVSTVIFKKFEPIFLDIFQNPYEEPPKLPRSRKQRRI----------PCSVKD 172

  Fly   189 LDDICWRLFLCAKNQKPSNTVDLVTSYNLMICCIDLIY-NNVLAEKRTDLINPKFEGLPSNWTEL 252
            |.:.||.||:..|........|||.||:|::||:|||: |.::...|.||:||.|:||||::...
Mouse   173 LFNFCWTLFVYTKGNFRMIGDDLVNSYHLLLCCLDLIFANAIMCPNRRDLLNPSFKGLPSDFHAP 237

  Fly   253 DFR--HNPHCILSNFCDMTE----EAKAMKATTFRQIMSSFFQASTIYGNKDTMLGLLANENFER 311
            ||:  ..|.||::..||:.:    |||.:|...|:..:|..|....:.|  :.:|.|   .:|..
Mouse   238 DFKAAEEPPCIIAVLCDLHDGLLVEAKGIKEHYFKPYISKLFDKKILKG--ECLLDL---SSFTD 297

  Fly   312 NLKSLNISYEQYVLSVGEFDERILSAYDAGEH-------TA------LNDQ-----SLR------ 352
            |.|::|..||:|||:||:|||||....||.|.       ||      |..|     :|:
Mouse   298 NSKAVNKEYEEYVLTVGDFDERIFLGADAEEEIGTPRKFTADTPFGKLTSQASVECNLQQHFEKK 362

  Fly   353 ---PPVTPLTRKQDLPAQPAMAGDKFEPVRNATNNVKQLSAF--GRITEPTDFVKQAGEEV---- 408
               .|.||||.::.|..:.|:.    .||.:||.:|.:|.:.  |..:.|::.:....|..
Mouse   363 RSFAPSTPLTGRRYLQEKEAVT----TPVASATQSVSRLQSIVAGLKSAPSEQLLNIFESCMRNP 423

  Fly   409 IAKLLSIIEEIEQKFLAKY-PSTE---------AKSRFQLAKSFFFYLLDQILQAEIRNKPDIDL 463
            :..::.|::.|.:.|...| .||:         |.:|.:||:..::.:|:.|:..|.|....:|:
Mouse   424 MGNIIKIVKGIGETFCQHYTQSTDKQPGSHIDFAVNRLKLAEILYYKILETIMVQETRRLHGMDM 488

  Fly   464 KRLLVQKVSLVIFNITLMACCVELVLEAYKTELKFPWVLDCFSISAFEFQKIIEIVVRHGSHEGC 528
            ..||.|.    ||:.:|||||:|:||.||.:...|||:::...:..|.|.|:||:|:|  |.||
Mouse   489 SVLLEQD----IFHKSLMACCLEIVLFAYSSPRTFPWIIEVLDLQPFYFYKVIEVVIR--SEEG- 546

  Fly   529 LNRSLIKHLNSIEETCLERLAWARNSTVWEMI-ASA-------------------------QLPL 567
            |:|.::||||||||..||.|||..||.:||.: |||                         .||:
Mouse   547 LSRDMVKHLNSIEEQILESLAWTNNSALWEALHASANRVPSCEEVIFPNNFEIGNGGNVQGHLPM 611

  Fly   568 ----------------------------------------------------------------- 567

Mouse   612 MPMSPIIHPRVKEVRTDSGSLRQDMQPLSPISVHERYSSPAAGSAKRRLFGDDPPKDTLMDKIMA 676

  Fly   568 ----------------------------------------------------------------- 567

Mouse   677 EGTKLKIAPSSVTAESLSISPGQALLTMATTTVTGTTGRKVTVPLHGIANDAGEITLVPISMNPT 741

  Fly   568 -------PTWLM------------------------VNLDRAAGPLQIFLRKVYLLGWLRIQKLC 601
                   |..|.                        |:..:..|.|.:|.||||.|..:|::.||
Mouse   742 QESTAESPVSLTAQSLIGTSPKQTHLTKAQDAHLTGVSKPKRTGSLALFYRKVYHLASVRLRDLC 806

  Fly   602 SELSLCEKTPESIWHIFEHSITHETELMKDRHLDQNIMCAIYIYIRVKRMEDPKFSDIMRAYRNQ 666
            .:|.:..:....||..||.::.|..:|||||||||.::||.||..:|.: |:..|.:||::||||
Mouse   807 LKLDVSNELRRKIWTCFEFTLVHCPDLMKDRHLDQLLLCAFYIMAKVTK-EERTFQEIMKSYRNQ 870

  Fly   667 PQAVNSVYREVFI-------------------DI-------NEDGEPKVK----DIIHFYNHTYV 701
            |||.:.|||.|.:                   ||       |...|| ||    |:|.|||..||
Mouse   871 PQANSHVYRSVLLKSIPGGVVVYNGDCEMTDGDIEDATKTPNCSSEP-VKEERGDLIKFYNTVYV 934

  Fly   702 PLMRQFVIDYLNVTPDVSGR-----ASDLQLSPHPKERAAQPKKVTQSHSLFVSQMSKNEIQQSP 761
            ..::.|.:.|     |:|.:     |..|...||.|::...|::::|.|||:||. .||....:|
Mouse   935 GRVKSFALKY-----DLSNQDHIMDAPPLSPFPHIKQQPGSPRRISQQHSLYVSP-HKNGAGLTP 993

  Fly   762 -NQMVYSFCRSPAKDLQAMNEKVRGG-----KRMLSF---GDEPGLGTMAETKRSKISQVKAVMD 817
             :.::|.|..||:|.|:.:|..:|.|     ||:::.   .|.|......|.....:.:::.|:.
Mouse   994 RSALLYKFNGSPSKSLKDINNMIRQGEQKTKKRVIAISGDADSPAKRLCQENDDVLLKRLQDVVS 1058

  Fly   818 D 818
            :
Mouse  1059 E 1059

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Rbf	NP_525036.2	DUF3452	90..225	CDD:288762	53/141 (38%)
		RB_A	376..560	CDD:280101	71/199 (36%)
		RB_B	582..704	CDD:280100	59/151 (39%)
Rbl1	NP_035379.2	DUF3452	77..209	CDD:288762	53/141 (38%)
		Pocket, binds T and E1A. /evidence=ECO:0000250\|UniProtKB:P28749	383..944		140/573 (24%)
		Domain A. /evidence=ECO:0000305	383..584		74/211 (35%)
		RB_A	385..578	CDD:280101	71/199 (36%)
		Spacer. /evidence=ECO:0000305	585..779		4/193 (2%)
		Domain B. /evidence=ECO:0000305	780..944		61/165 (37%)
		RB_B	786..937	CDD:280100	59/152 (39%)
		Rb_C	956..>1041	CDD:286075	27/85 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167837302
Domainoid	1	1.000	113	1.000	Domainoid score	I6112
eggNOG	1	0.900	-	-		E1_KOG1010
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	447	1.000	Inparanoid score	I1615
Isobase	1	0.950	-	0	Normalized mean entropy	S7132
OMA	1	1.010	-	-		QHG46367
OrthoDB	1	1.010	-	-		D113612at2759
OrthoFinder	1	1.000	-	-		FOG0002362
OrthoInspector	1	1.000	-	-		otm42489
orthoMCL	1	0.900	-	-		OOG6_103769
Panther	1	1.100	-	-	O	PTHR13742
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3203
SonicParanoid	1	1.000	-	-		X2053
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	17	16.750