DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Rbf and Rb1

DIOPT Version :9

Sequence 1:	NP_525036.2	Gene:	Rbf / 31027	FlyBaseID:	FBgn0015799	Length:	845	Species:	Drosophila melanogaster
Sequence 2:	NP_033055.2	Gene:	Rb1 / 19645	MGIID:	97874	Length:	921	Species:	Mus musculus

Alignment Length:	981	Identity:	221/981 - (22%)
Similarity:	376/981 - (38%)	Gaps:	252/981 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 EPDP-QELGAEVVSGLVATSDDRLEMINAEYTNLCRDLNMDRQTELQGYETYLEVSQRCSMEGTA 66
            |.|| |:.|.|.:. |.....:.:|  ..|:..||:.|.:......:.:.|:.:||   |::|..
Mouse    24 EDDPAQDSGPEELP-LARLEFEEIE--EPEFIALCQKLKVPDHVRERAWLTWEKVS---SVDGIL 82

  Fly    67 SH--------WMCCAIYTACRRTSTPTVTGQNAVVKGNCVSLNNLLRCCKMSIYEFKTKIKQWCD 123
            ..        |..|....|......|             .:...|.:..:.|:|:|...:|:
Mouse    83 EGYIQKKKELWGICIFIAAVDLDEMP-------------FTFTELQKSIETSVYKFFDLLKE--- 131

  Fly   124 MANLPQEFVNEIEDLDRKFSITFMLHKRFRIIMDMIFSCPPNEKKHSKYISLHGNHAHGKCSYIK 188
             .:...:..|.:..|.:|:::...|:.:.....::|:...|     |..:|...|      |.:.
Mouse   132 -IDTSTKVDNAMSRLLKKYNVLCALYSKLERTCELIYLTQP-----SSALSTEIN------SMLV 184

  Fly   189 LDDICWRLFLCAKNQKPSNTVDLVTSYNLMICCIDLIYNNVLAEKRTDLINP-KFEGLPSNWTEL 252
            | .|.|..||.||.:......|||.|:.||:|.:|..   :.......|..| |...:|.|.:..
Mouse   185 L-KISWITFLLAKGEVLQMEDDLVISFQLMLCVVDYF---IKFSPPALLREPYKTAAIPINGSPR 245

  Fly   253 DFR-------------HNPHCILSNFCDMTE----EAKAMKATTFRQIMSSFFQASTIYGNKDTM 300
            ..|             .|...|:...|...|    |.|.:....|...::|
Mouse   246 TPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFINS-------------- 296

  Fly   301 LGLLANENFERNLKSLNISYEQYVLSVGEFDERILSAYDAGEHTALNDQSLRPPV----TPLTRK 361
            ||::::.... .::||:..||:..|...:.|.|:...:|....|        .|:    |..|.:
Mouse   297 LGIVSSNGLP-EVESLSKRYEEVYLKNKDLDARLFLDHDKTLQT--------DPIDSFETERTPR 352

  Fly   362 QDLPAQPAMAGDKFEPVRNATNNVKQLSAFGRITEPTDFVKQAGEEVIA-----------KLLSI 415
            ::.|.:.|.......|||...|.::||...  :...:|   |..|.:|:           .:|..
Mouse   353 KNNPDEEANVVTPHTPVRTVMNTIQQLMVI--LNSASD---QPSENLISYFNNCTVNPKENILKR 412

  Fly   416 IEEIEQKFLAKYPSTEAK-------SRFQLAKSFFFYLLDQILQAEIRNKPDIDLKRLLVQKVSL 473
            ::::...|..|:.:...:       .|::|....::.:::.:|::|        .:||.:|..|.
Mouse   413 VKDVGHIFKEKFANAVGQGCVDIGVQRYKLGVRLYYRVMESMLKSE--------EERLSIQNFSK 469

  Fly   474 V----IFNITLMACCVELVLEAYK----------TELKFPWVLDCFSISAFEFQKIIEIVVRHGS 524
            :    ||:::|:||.:|:|:..|.          |:|.|||:|:..::.||:|.|:||..::
Mouse   470 LLNDNIFHMSLLACALEVVMATYSRSTLQHLDSGTDLSFPWILNVLNLKAFDFYKVIESFIK--- 531

  Fly   525 HEGCLNRSLIKHLNSIEETCLERLAWARNSTVWEMIASAQ-----------LPLPTWLMVN---L 575
            .|..|.|.:||||...|...:|.|||..:|.::::|..::           .||...|..|   .
Mouse   532 VEANLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDGEGPDNLEPACPLSLPLQGNHTAA 596

  Fly   576 DRAAGPLQ-------------------------------------IFLRKVYLLGWLRIQKLCSE 603
            |....||:                                     :|.:|||.|.:||:..||:.
Mouse   597 DMYLSPLRSPKKRTSTTRVNSAANTETQAASAFHTQKPLKSTSLALFYKKVYRLAYLRLNTLCAR 661

  Fly   604 LSLCEKTPE---SIWHIFEHSITHETELMKDRHLDQNIMCAIYIYIRVKRMEDPKFSDIMRAYRN 665
              |....||   .||.:|:|::.:|.|||:||||||.:||::|...:||.: |.||..|:.||::
Mouse   662 --LLSDHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNI-DLKFKIIVTAYKD 723

  Fly   666 QPQAVNSVYREVFIDINEDGEPKVKDIIHFYNHTYVPLMRQFVIDYLNVTPDVSGRASDLQLSPH 730
            .|.|....::.|.|     .|.:...||.|||..::..::..::.|.:..|........:..||:
Mouse   724 LPHAAQETFKRVLI-----REEEFDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPY 783

  Fly   731 P------------------------KERAAQPKKVTQSHSLFVS---QMSKNEIQQSPNQMVYSF 768
            .                        .|....|.|:|....:.||   ....:|..|..||||
Mouse   784 KFSSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMV--- 845

  Fly   769 CRS------------PAKDLQAMNEKVRGGKRMLSFGDEPGLGTMAETKRSKISQVKAVMDDPEL 821
            |.|            |.|.|:.:...:.|       .||.. |:......||..|..|.|.....
Mouse   846 CNSDRVLKRSAEGGNPPKPLKKLRFDIEG-------ADEAD-GSKHLPAESKFQQKLAEMTSTRT 902

  Fly   822 QSAEQQ 827
            :..:|:
Mouse   903 RMQKQR 908

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Rbf	NP_525036.2	DUF3452	90..225	CDD:288762	30/134 (22%)
		RB_A	376..560	CDD:280101	54/215 (25%)
		RB_B	582..704	CDD:280100	48/161 (30%)
Rb1	NP_033055.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..36		6/11 (55%)
		DUF3452	112..219	CDD:371807	29/122 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	341..360		4/18 (22%)
		Pocket, binds T and E1A. /evidence=ECO:0000250	367..764		109/420 (26%)
		Domain A. /evidence=ECO:0000250	367..573		55/221 (25%)
		RB_A	367..567	CDD:366846	54/215 (25%)
		Spacer. /evidence=ECO:0000250	574..632		7/57 (12%)
		Domain B. /evidence=ECO:0000250	633..764		47/138 (34%)
		RB_B	639..758	CDD:366845	47/126 (37%)
		Interaction with LIMD1. /evidence=ECO:0000250	756..921		31/164 (19%)
		Rb_C	761..917	CDD:370194	31/159 (19%)
		Domain, mediates interaction with E4F1. /evidence=ECO:0000250	764..921		31/156 (20%)
		Bipartite nuclear localization signal. /evidence=ECO:0000269\|PubMed:8336704	853..869		3/15 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	872..921		10/45 (22%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167837298
Domainoid	1	1.000	113	1.000	Domainoid score	I6112
eggNOG	1	0.900	-	-		E1_KOG1010
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D113612at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR13742
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	6	5.850