DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Appl and App

DIOPT Version :9

Sequence 1:	NP_001245448.1	Gene:	Appl / 31002	FlyBaseID:	FBgn0000108	Length:	890	Species:	Drosophila melanogaster
Sequence 2:	NP_062161.1	Gene:	App / 54226	RGDID:	2139	Length:	770	Species:	Rattus norvegicus

Alignment Length:	951	Identity:	240/951 - (25%)
Similarity:	379/951 - (39%)	Gaps:	263/951 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 LLLRSLWVVLAI-----GTAQVQAASPRWEPQIAVLCEAGQIYQPQYLSEEGRWVTDLSKKTTGP 68
            |||.:.|.|.|:     |.|.:.|     |||||:.|  |::.....: :.|:|.:|.|..   .
  Rat     7 LLLLAAWTVRALEVPTDGNAGLLA-----EPQIAMFC--GKLNMHMNV-QNGKWESDPSGT---K 60

  Fly    69 TCLRDKMDLLDYCKKAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCL-GPF 132
            ||:..|..:|.||::.||...|||:||::....|..||::|   ..:||.....:.|:||| |.|
  Rat    61 TCIGTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRG---RKQCKTHTHIVIPYRCLVGEF 122

  Fly   133 QSDALLVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCC 197
            .|||||||:.|.|.|......|...:.|:......|.|:...:..:.|||||||..|.|||||||
  Rat   123 VSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCC 187

  Fly   198 PKHFKTDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSNDSNYSKDANEDDLD------DEDDL 256
            |                               :.:|.||.|   |.||.|||.|      |.|..
  Rat   188 P-------------------------------LAEESDSID---SADAEEDDSDVWWGGADTDYA 218

  Fly   257 MGDDEEDDMVADEAATAGGSPNTGSSGDSNSGSLDDINAEYDSGEEGDNYEEDGAGSESEAEVEA 321
            .|.:::...||:|...|                    :.|.:..|:.::.|:   |.|.|.|.|.
  Rat   219 DGGEDKVVEVAEEEEVA--------------------DVEEEEAEDDEDVED---GDEVEEEAEE 260

  Fly   322 SWDQSGGAKVVSLKSDSSSPSSAPVAPAPEKAPVKSES-------------VT------------ 361
            .:::: ..:..|:.:.:::.:.:......|....::|:             ||
  Rat   261 PYEEA-TERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGC 324

  Fly   362 -------STPQLSASAAAFVAANSGNSGTGAGAP------PSTAQPTS---DPYFTHFDPHYEHQ 410
                   .|.:...:....|::.|....|....|      |:||..|.   |.|........||.
  Rat   325 GGNRNNFDTEEYCMAVCGSVSSQSLLKTTSEPLPQDPVKLPTTAASTPDAVDKYLETPGDENEHA 389

  Fly   411 SYKVSQKRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFKQRMTARFQTSVQALEEEGN 475
            .::.:::|||..|||::::||::|.:.|.:.:::..||.||       :...||..|::||:|..
  Rat   390 HFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKA-------VIQHFQEKVESLEQEAA 447

  Fly   476 AEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKCLQKLLRALHKDRAHALAHY 540
            .|:.||...|..||.|.:|.|:|.|:..|..||...||..|||...|:|.:||..|||.|.|.|:
  Rat   448 NERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTLKHF 512

  Fly   541 RHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYPELSAKIAQLMNDYILALRSKD 605
            .| :....|   :.||..|.:.:..|..|...:|||:::|...|.::.:|             :|
  Rat   513 EH-VRMVDP---KKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEI-------------QD 560

  Fly   606 DIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQRAAEREKLREEKLRLEAKKV 670
            ::                                                    :|.|:.|....
  Rat   561 EV----------------------------------------------------DELLQKEQNYS 573

  Fly   671 DDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVTAANPNLETTKS-------- 727
            ||:|.:.::|                       |....|.||.:     |:|..||:
  Rat   574 DDVLANMISE-----------------------PRISYGNDALM-----PSLTETKTTVELLPVN 610

  Fly   728 -EKDLSDTE----YGEATVSTKVQTVLPTVD-DDAVQRAVED-VAAAVAHQEAEPQVQHFMTHDL 785
             |..|.|.:    :|..:|....:..:..|| ..|..|.:.. ..:.:.:.:.|...:..|..:.
  Rat   611 GEFSLDDLQPWHPFGVDSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEF 675

  Fly   786 GHRESSFSLRRE----FAQHAHAAKEGRNVYFTLSFAGIALMAAVFVGVAVAKWRTSRSPHAQGF 846
            || :|.|.:|.:    ||:...:.|   .....|...|:.:...:.:.:.:.|.:...|.| .|.
  Rat   676 GH-DSGFEVRHQKLVFFAEDVGSNK---GAIIGLMVGGVVIATVIVITLVMLKKKQYTSIH-HGV 735

  Fly   847 IEVDQVGCQNVTTHHPIVREEKIVPNMQINGYENPTYKYFE 887
            :|||..          :..||:.:..||.|||||||||:||
  Rat   736 VEVDAA----------VTPEERHLSKMQQNGYENPTYKFFE 766

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Appl	NP_001245448.1	A4_EXTRA	26..198	CDD:128326	65/172 (38%)
		APP_N	33..141	CDD:280358	42/108 (39%)
		APP_Cu_bd	143..198	CDD:289676	21/54 (39%)
		APP_E2	387..580	CDD:289677	68/201 (34%)
		GBP_C	<629..682	CDD:303769	7/52 (13%)
		APP_amyloid	834..887	CDD:287486	20/52 (38%)
App	NP_062161.1	A4_EXTRA	24..188	CDD:128326	67/177 (38%)
		GFLD subdomain. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01217	28..123		36/108 (33%)
		Heparin-binding. /evidence=ECO:0000250\|UniProtKB:P05067	96..110		5/16 (31%)
		CuBD subdomain. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01217	131..189		23/88 (26%)
		Copper-binding. /evidence=ECO:0000250	135..155		4/19 (21%)
		Zinc-binding. /evidence=ECO:0000250	181..188		6/6 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	196..283		24/113 (21%)
		Kunitz_BPTI	294..341	CDD:333766	4/46 (9%)
		OX-2. /evidence=ECO:0000250\|UniProtKB:P05067	344..365		4/20 (20%)
		APP_E2	366..548	CDD:372388	66/192 (34%)
		Heparin-binding. /evidence=ECO:0000250	391..423		10/31 (32%)
		Heparin-binding. /evidence=ECO:0000250	491..522		12/34 (35%)
		Collagen-binding. /evidence=ECO:0000250\|UniProtKB:P05067	523..540		5/16 (31%)
		Beta-APP	677..713	CDD:367525	9/39 (23%)
		Interaction with PSEN1. /evidence=ECO:0000250\|UniProtKB:P05067	695..722		3/29 (10%)
		APP_amyloid	716..766	CDD:371108	20/60 (33%)
		Basolateral sorting signal. /evidence=ECO:0000250	724..734		3/10 (30%)
		Interaction with G(o)-alpha	732..751		7/29 (24%)
		Required for the interaction with KIF5B and for anterograde transport in axons. /evidence=ECO:0000250\|UniProtKB:P05067	756..770		10/11 (91%)
		YENPXY motif, contains endocytosis signal. /evidence=ECO:0000250\|UniProtKB:P05067	757..762		4/4 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166347497
Domainoid	1	1.000	108	1.000	Domainoid score	I6291
eggNOG	1	0.900	-	-		E1_KOG4295
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	226	1.000	Inparanoid score	I3397
OMA	1	1.010	-	-		QHG50020
OrthoDB	1	1.010	-	-		D405630at33208
OrthoFinder	1	1.000	-	-		FOG0001588
OrthoInspector	1	1.000	-	-		otm45349
orthoMCL	1	0.900	-	-		OOG6_104222
Panther	1	1.100	-	-	O	PTHR23103
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X996
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	14	13.860