DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Appl and APP

DIOPT Version :9

Sequence 1:	NP_001245448.1	Gene:	Appl / 31002	FlyBaseID:	FBgn0000108	Length:	890	Species:	Drosophila melanogaster
Sequence 2:	NP_000475.1	Gene:	APP / 351	HGNCID:	620	Length:	770	Species:	Homo sapiens

Alignment Length:	955	Identity:	235/955 - (24%)
Similarity:	369/955 - (38%)	Gaps:	271/955 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 LLLRSLWVVLAI-----GTAQVQAASPRWEPQIAVLCEAGQIYQPQYLSEEGRWVTDLSKKTTGP 68
            |||.:.|...|:     |.|.:.|     |||||:.|  |::.....: :.|:|.:|.|..   .
Human     7 LLLLAAWTARALEVPTDGNAGLLA-----EPQIAMFC--GRLNMHMNV-QNGKWDSDPSGT---K 60

  Fly    69 TCLRDKMDLLDYCKKAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCL-GPF 132
            ||:..|..:|.||::.||...|||:||::....|..||::|   ..:||....::.|:||| |.|
Human    61 TCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRG---RKQCKTHPHFVIPYRCLVGEF 122

  Fly   133 QSDALLVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCC 197
            .|||||||:.|.|.|......|...:.|:......|.|:...:..:.|||||||..|.|||||||
Human   123 VSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCC 187

  Fly   198 PKHFKTDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSNDSNYSKDANEDDLD------DEDDL 256
            |                               :.:|.|:.|   |.||.|||.|      |.|..
Human   188 P-------------------------------LAEESDNVD---SADAEEDDSDVWWGGADTDYA 218

  Fly   257 MGDDEEDDMVADEAATAGGSPNTGSSGDSNSGSLDDINAEYDSGEEGDNYEEDGAGSESEAEVEA 321
            .|.:::...||:|...                      ||.:. ||.|:.|:|..|.|.|.|.|.
Human   219 DGSEDKVVEVAEEEEV----------------------AEVEE-EEADDDEDDEDGDEVEEEAEE 260

  Fly   322 SWDQSGGAKVVSLKSDSSSPSSAPVAPAPEKAPVKSESVTSTPQLS---------ASAAAFVAAN 377
            .:::: ..:..|:.:.:::.:.:......|....::|:......:|         ..|..|....
Human   261 PYEEA-TERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGC 324

  Fly   378 SGNSGT-----------GAGAPPSTAQPTSDP---------------------YFTHFDPHYEHQ 410
            .||...           |:....|..:.|.:|                     |........||.
Human   325 GGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHA 389

  Fly   411 SYKVSQKRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFKQRMTARFQTSVQALEEEGN 475
            .::.:::|||..|||::::||::|.:.|.:.:::..||.||       :...||..|::||:|..
Human   390 HFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKA-------VIQHFQEKVESLEQEAA 447

  Fly   476 AEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKCLQKLLRALHKDRAHALAHY 540
            .|:.||...|..||.|.:|.|:|.|:..|..||...||...||...|:|.:||..|||.|.|.|:
Human   448 NERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHF 512

  Fly   541 RHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYPELSAKIAQLMNDYILALRSKD 605
            .| :....|   :.||..|.:.:..|..|...:|||:::|...|.::.:|             :|
Human   513 EH-VRMVDP---KKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEI-------------QD 560

  Fly   606 DIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQRAAEREKLREEKLRLEAKKV 670
            ::                                                    :|.|:.|....
Human   561 EV----------------------------------------------------DELLQKEQNYS 573

  Fly   671 DDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVTAANPNLETTKS-------- 727
            ||:|.:.::|                       |....|.||.:     |:|..||:
Human   574 DDVLANMISE-----------------------PRISYGNDALM-----PSLTETKTTVELLPVN 610

  Fly   728 -EKDLSDTE----YGEATVSTKVQTVL------PTVDDDAVQRAVEDVAAAVAHQEAEPQVQHFM 781
             |..|.|.:    :|..:|....:..:      |..|.....|....:......:.:|.::....
Human   611 GEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEF 675

  Fly   782 THDLG----HRESSFSLRREFAQHAHAAKEGRNVYFTLSFAGIALMAAVFVGVAVAKWRTSRSPH 842
            .||.|    |::..|     ||:...:.|   .....|...|:.:...:.:.:.:.|.:...|.|
Human   676 RHDSGYEVHHQKLVF-----FAEDVGSNK---GAIIGLMVGGVVIATVIVITLVMLKKKQYTSIH 732

  Fly   843 AQGFIEVDQVGCQNVTTHHPIVREEKIVPNMQINGYENPTYKYFE 887
             .|.:|||..          :..||:.:..||.|||||||||:||
Human   733 -HGVVEVDAA----------VTPEERHLSKMQQNGYENPTYKFFE 766

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Appl	NP_001245448.1	A4_EXTRA	26..198	CDD:128326	65/172 (38%)
		APP_N	33..141	CDD:280358	42/108 (39%)
		APP_Cu_bd	143..198	CDD:289676	21/54 (39%)
		APP_E2	387..580	CDD:289677	64/213 (30%)
		GBP_C	<629..682	CDD:303769	7/52 (13%)
		APP_amyloid	834..887	CDD:287486	20/52 (38%)
APP	NP_000475.1	A4_EXTRA	24..188	CDD:128326	67/177 (38%)
		GFLD subdomain. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01217	28..123		36/108 (33%)
		Heparin-binding. /evidence=ECO:0000269\|PubMed:8158260	96..110		5/16 (31%)
		CuBD subdomain. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01217	131..189		23/88 (26%)
		Zinc-binding	181..188		6/6 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	194..284		28/116 (24%)
		Kunitz_BPTI	294..341	CDD:333766	6/46 (13%)
		OX-2. /evidence=ECO:0000269\|PubMed:2649245	344..365		3/20 (15%)
		APP_E2	366..548	CDD:372388	61/192 (32%)
		Heparin-binding	391..423		10/31 (32%)
		Heparin-binding	491..522		12/34 (35%)
		Collagen-binding. /evidence=ECO:0000269\|PubMed:8576160	523..540		5/16 (31%)
		Beta-APP	677..713	CDD:367525	10/43 (23%)
		Interaction with PSEN1. /evidence=ECO:0000269\|PubMed:30630874	695..722		3/29 (10%)
		APP_amyloid	716..766	CDD:371108	20/60 (33%)
		Basolateral sorting signal	724..734		3/10 (30%)
		Interaction with G(o)-alpha	732..751		7/29 (24%)
		Required for the interaction with KIF5B and for anterograde transport in axons. /evidence=ECO:0000269\|PubMed:17062754	756..770		10/11 (91%)
		YENPXY motif, contains endocytosis signal. /evidence=ECO:0000269\|PubMed:10383380	757..762		4/4 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165153901
Domainoid	1	1.000	108	1.000	Domainoid score	I6423
eggNOG	1	0.900	-	-		E1_KOG4295
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	223	1.000	Inparanoid score	I3526
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG50020
OrthoDB	1	1.010	-	-		D405630at33208
OrthoFinder	1	1.000	-	-		FOG0001588
OrthoInspector	1	1.000	-	-		otm41214
orthoMCL	1	0.900	-	-		OOG6_104222
Panther	1	1.100	-	-	O	PTHR23103
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2378
SonicParanoid	1	1.000	-	-		X996
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	14	13.890