Protein Alignment Appl and APLP1

DIOPT Version :10

• Sequence 1: NP_001245448.1
• Gene: Appl / 31002
• FlyBaseID: FBgn0000108
• Length: 890
• Species: Drosophila melanogaster
• Sequence 2: NP_001019978.1
• Gene: APLP1 / 333
• HGNCID: 597
• Length: 651
• Species: Homo sapiens
• Alignment Length: 891
• Identity: 203/891 - (22%)
• Similarity: 318/891 - (35%)
• Gaps: 283/891 - (31%)

  Fly     9 LLLRSLWVVLAIGTAQVQAASPRWEPQIAVLCEAGQIYQPQYLSEEGRWVTDLSKKTTGPTCLRD 73
            ||||:...:.::......||......|:|.||  |::...:.| ..|||..|..:..   .||||
Human    29 LLLRAQPAIGSLAGGSPGAAEAPGSAQVAGLC--GRLTLHRDL-RTGRWEPDPQRSR---RCLRD 87

  Fly    74 KMDLLDYCKKAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCL-GPFQSDAL 137
            ...:|:||::.||...|..:.:::....:..||  |...:..|...|..:.||||| |.|.|:||
Human    88 PQRVLEYCRQMYPELQIARVEQATQAIPMERWC--GGSRSGSCAHPHHQVVPFRCLPGEFVSEAL 150

  Fly   138 LVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCCPKHFK 202
            ||||||.|.|.....:|....|.:|....||..:|:.:....||||||...|.|||:||||.
Human   151 LVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPP--- 212

  Fly   203 TDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSNDSNYSKDANEDDLDDEDDLMGDDEEDDMVA 267
                       |..|
Human   213 -----------PGTP-------------------------------------------------- 216

  Fly   268 DEAATAGGSPNTGSSGDSNSGSLDDINAEYDSGEEGDNYEEDGAGSESEAEVEASWDQSGGAKVV 332
            |.:.||.|.|:|                  .|...|...|    |:|.|.| |.|:.|
Human   217 DPSGTAVGDPST------------------RSWPPGSRVE----GAEDEEE-EESFPQ------- 251

  Fly   333 SLKSDSSSPSSAPVAPAPEKAPVKSESVTSTPQLSASAAAFVAANSGNSGTGAGAPPSTAQPTS- 396
                        ||.....:.|...|...:.|..|:...|.|           |....|.:||.
Human   252 ------------PVDDYFVEPPQAEEEEETVPPPSSHTLAVV-----------GKVTPTPRPTDG 293

  Fly   397 -DPYFTHFDPHYEHQSYKVSQKRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFKQRMT 460
             |.||.......||:.:..::..|||....::..||::|:..:.:.:::..||       :|.:.
Human   294 VDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKAD-------RQALN 351

  Fly   461 ARFQTSVQALEEEGNAEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKCLQKL 525
            ..||:.:|.|||:.:.|:.:|...|..||:|.||.::|.|:..:..||...||.|..|...|::.
Human   352 EHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRY 416

  Fly   526 LRALHKDRAHALAHYRHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYP----EL 586
            |||..|::.|.|.||:|:. :..|   |.|...|.:....|..|:..||||:.:|.:.|    ||
Human   417 LRAEQKEQRHTLRHYQHVA-AVDP---EKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQEL 477

  Fly   587 SAKIAQLMNDYILALRSKDDIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQR 651
            ..:|.:|::...|   ...::...:.|.|.|.:.|:...
Human   478 RPQIQELLHSEHL---GPSELEAPAPGGSSEDKGGLQPP-------------------------- 513

  Fly   652 AAEREKLREEKLRLEAKKVDDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVT 716
                                   .|:.|:......:.||:..|...::||..|
Human   514 -----------------------DSKDADTPMTLPKGSTEQDAASPEKEKMNP------------ 543

  Fly   717 AANPNLETTKSEKDLSDTEYGEATVSTKVQTVLPTVDDDAVQRAVEDVAAAVAHQEAEPQVQHFM 781
                             .|..|..|:..|....| .....:||  :::|.|
Human   544 -----------------LEQYERKVNASVPRGFP-FHSSEIQR--DELAPA-------------- 574

  Fly   782 THDLGHRESSFSLRREFAQHAHAAKEGRNVYFTLSFAGIALMAA-----VFVGVAVAKWRTSRSP 841
                     ...:.||                  :.:|:.:|.|     :.:.:.:.:.:.....
Human   575 ---------GTGVSRE------------------AVSGLLIMGAGGGSLIVLSMLLLRRKKPYGA 612

  Fly   842 HAQGFIEVDQVGCQNVTTHHPIVREEKIVPNMQINGYENPTYKYFE 887
            .:.|.:|||.:          :..||:.:..:|.:|||||||::.|
Human   613 ISHGVVEVDPM----------LTLEEQQLRELQRHGYENPTYRFLE 648

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Appl	NP_001245448.1	A4_EXTRA	26..198	CDD:128326	63/172 (37%)
		APP_E2	388..580	CDD:463752	59/193 (31%)
		DDRGK	632..>682	CDD:370664	2/49 (4%)
		APP_amyloid	834..887	CDD:463129	14/52 (27%)
APLP1	NP_001019978.1	A4_EXTRA	46..211	CDD:128326	63/172 (37%)
		GFLD subdomain. /evidence=ECO:0000255|PROSITE-ProRule:PRU01217	50..146		31/103 (30%)
		CuBD subdomain. /evidence=ECO:0000255|PROSITE-ProRule:PRU01217	154..212		24/57 (42%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	214..287		26/175 (15%)
		APP_E2	285..467	CDD:463752	59/192 (31%)
		O-glycosylated at three sites	285..305		6/19 (32%)
		Heparin-binding. /evidence=ECO:0000250	310..342		6/31 (19%)
		Heparin-binding. /evidence=ECO:0000250	410..441		11/34 (32%)
		Collagen-binding. /evidence=ECO:0000250	442..459		4/16 (25%)
		JMTM_APLP1	565..649	CDD:411991	24/137 (18%)
		Basolateral sorting signal. /evidence=ECO:0000250	605..616		0/10 (0%)
		Interaction with DAB1. /evidence=ECO:0000250	633..650		9/16 (56%)
		Interaction with DAB2. /evidence=ECO:0000250	637..651		8/12 (67%)
		Clathrin-binding. /evidence=ECO:0000255	641..644		2/2 (100%)
		NPXY motif, contains endocytosis signal	641..644		2/2 (100%)