back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo3 and Dscam2

DIOPT Version :10

Sequence 1:	XP_005172693.1	Gene:	robo3 / 30770	ZFINID:	ZDB-GENE-000209-4	Length:	1424	Species:	Danio rerio
Sequence 2:	NP_001261500.1	Gene:	Dscam2 / 38788	FlyBaseID:	FBgn0265296	Length:	2101	Species:	Drosophila melanogaster

Alignment Length:	1639	Identity:	368/1639 - (22%)
Similarity:	575/1639 - (35%)	Gaps:	481/1639 - (29%)

- Green bases have known domain annotations that are detailed below.

Zfish    57 RLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGS 121
            :|.||.|.::....:..:..|...:|.|.|.|.|||.:.|..||              ..|||..
  Fly   417 QLGDAPPVLLYSFIEQTLQPGPAVSLKCSAAGNPTPQISWTLDG--------------FPLPSNG 467

Zfish   122 LFFLR---IVHG---------RRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDV 174
            .|.:.   .|||         .....|.|.|.|:|.|..|. |...|.|.:..| ...|..|. |
  Fly   468 RFMIGQYITVHGDVISHVNISHVMVEDGGEYACIAENRAGR-VQHAARLNIYGL-PYIRLIPK-V 529

Zfish   175 VVAAGEPAVMECIPPRGHPEPTISWKRNNVKVNDKDERITIRGGKLMISNTRK-SDAGMYVCVGT 238
            ...:||...::| |..|:|...|.|:|...::.|...:.....|.|.||..:| ||:|:|.|...
  Fly   530 TAVSGETLNLKC-PVAGYPIEEIHWERGGRELPDDIRQRVQPDGSLTISPVQKNSDSGVYTCWAR 593

Zfish   239 NMVGEKDSDPAELVVFERPMFVRRPVNQVVL-ADDTVDFQCE-VQGDPAPTIRWKKEEGEL-PRG 300
            |..|.......|:.|...|.......|.:.| ..|.....|. |:||...||.|:|:...: |..
  Fly   594 NKQGHSARRSGEVTVIVPPKLSPFQTNILQLNMGDRASLTCSVVKGDLPLTINWRKDGRPIDPTQ 658

Zfish   301 RFEIRA----DNSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVGPSLPPQIVVRPRDQIS 361
            ...::.    ::.|.:..:.::..|:|:|:..||..:.|.|.:|.|:|    ||:.:|.|.|...
  Fly   659 HMSVKQVDQYNSILVIENLGSDHTGNYSCVVRNSAAEVENSQALLVNV----PPRWIVEPVDANV 719

Zfish   362 AQGRTVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRF---------SVSLSGELTITDV 417
            .:.|.:...|..:|.|.|::.|:|...          |:||.:         .:..:|.|.:..|
  Fly   720 ERNRHIMLHCQAQGVPTPSIVWKKATG----------SKSGEYEEVRERPFTKLLGNGSLLLQHV 774

Zfish   418 HSEDSGYYICQAISVAGSILTKAL-LEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNP 481
            ..:..|:|:|||.:..|:.:.|.: |:|.|:      |.......:..:..|.||.|||.|.|:.
  Fly   775 KEDREGFYLCQANNGIGTGIGKVIQLKVNSS------PYFSSTSRSVMVKKGDTALLQCAVSGDK 833

Zfish   482 LPSIQWERDGQRIL--GIDERISLMENGT-------LQITALQETDSGAYTCVASSLSGETSWSG 537
            ..:|.|.|.|:..|  ..:.:||:.:..|       |||..:..||||.|.|.||:|.|......
  Fly   834 PINIVWMRSGKNTLNPSTNYKISVKQEATPDGVSAELQIRTVDATDSGPYFCRASNLYGNDQQLV 898

Zfish   538 VLTVKESGGLSASPVSEPYQLPGPPQKPVVTD---VTRNSVTLTWQPNAHEGGAAVTSYIIEAFS 599
            .|.|:|                 ||..|.|.:   ::..||.:.|||.. .|...||.||:| |.
  Fly   899 QLQVQE-----------------PPLPPSVLEAAMISSRSVNIKWQPKT-LGTGDVTKYIVE-FR 944

Zfish   600 QSAGS--------TWQTVADFVKLEKH---TVTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRT 653
            ::..|        .||.:.  ||...|   .:..|.|.|.|.|.|.|..:.|.|.||. ...|||
  Fly   945 EADHSLPPALFVDQWQQIE--VKDPPHFNAMIENLKPATRYAFRVIAEGSAGRSAPSQ-ELIVRT 1006

Zfish   654 QDVSPTGQGVDHRQVQRELGEMSVQLHEPVL------LTASSVKISWNV---DRQSQYIQGYRLL 709
            :...|.|                     |.|      |:::.:.|||..   :.:...||||.:.
  Fly  1007 EPQRPAG---------------------PPLSLSARPLSSTELLISWVAPLPELRHGDIQGYNVG 1050

Zfish   710 YRPVGG---SWSQQEVKG---ATERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEV 768
            |:....   :::...|.|   ......:::.|.|...|.:.::.:.....|..|.....:|.|:|
  Fly  1051 YKLSSSGNTAYNFTSVSGDGDGGNGELLLSGLAKFARYTVVVQAFNQVGPGPLSEPTAAQTMEDV 1115

Zfish   769 PSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWC--LGNDSQTRYNINQTVDGSV 831
            ||.||..|..|.:   :|.|:.|||||||....||::|.||:..  :.:|.|...:..::...:.
  Fly  1116 PSRPPEDVRCAAL---SSQSLQVSWQPPPIYHTNGLLQGYKLIFEPIIDDIQPSKDEVESRKTTA 1177

Zfish   832 LSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITD 896
            |:.||.||.....|.::|.|.|..|.|..|:|:.                ..|||:|..|.....
  Fly  1178 LTMVLTGLRKYTNYSIQVLAHTRMGDGVVSKPLF----------------CHSEEDVPEAPADIK 1226

Zfish   897 VV---KQPAFIAGIGGACWV-------ILMGFSVWIYCR-RKKRKELSHYTASF----AYTPAVG 946
            ||   .|..:|:      |:       ::..:|  :|.| ...|:||::...|.    ||..|.|
  Fly  1227 VVSSSSQSLYIS------WLPPNEPNGVITKYS--LYTRVVNGREELNNEKRSLPSQQAYYEAKG 1283

Zfish   947 F-PH---------------GESSGLN----------------------------------GRP-- 959
            . ||               |:||.::                                  |:|
  Fly  1284 LHPHMEYQFWVTASTRVGEGKSSRVSSQITTNRIPARIISFGGPVVRPWRSTVTLPCTAVGKPKR 1348

Zfish   960 ------------GMLGANM----------------GNYPWLADSWPTTN---------------- 980
                        |:..:.:                |||....|:...|:
  Fly  1349 EWFKSDVALRQGGLHNSQLLDSGDLIISSLQLADGGNYSCQVDNGIGTDRLTHTLIVQVPPTAPV 1413

Zfish   981 -----------LVH-----NGKDSVNCCTSKHETPERYYNEAGISNYLNQNE------------K 1017
                       |:|     .|...:...|..:.......:|..:|.:.:.:|            .
  Fly  1414 LYVTSATSSSILMHWKCGFTGNAPITGYTLFYRRANGNTDEMQLSRHASSHELKGLMCGSTYQIH 1478

Zfish  1018 YSADSNEGPIYSTIDPTSEDLRSFTAQYSQ--------------------HTTPYASTPIMPFTN 1062
            .||.:..|     ..|||..|...|...|.                    |:.|....|::.|..
  Fly  1479 LSAQNKVG-----TSPTSTILHVRTQGQSPGHPASTALLAPNSTSLLVRLHSWPDNGCPLLYFVL 1538

Zfish  1063 QAPCSPEQDGSHWMTQPGTSNAQYAQPDRSKTENVKQPK---------QKLMGKPVKTPSLNWM- 1117
            |.....:...:.|:.   .|||  .:|.|....|..||.         ..:.|  :.....|::
  Fly  1539 QYRAVTDDPDAEWVL---VSNA--LKPQRRIVINNLQPSTLYQLRMEAHNVAG--ISQAEFNFVT 1596

Zfish  1118 ---DALPPPP-------------ISGDLEQCDEEDDNELIQDMGSDEEEWC-------------- 1152
               |..||||             |..::        |.||..:.:....:|
  Fly  1597 LTKDGDPPPPEIMHRGRSGQTTVIFANI--------NLLIPTIAAVSGMFCTIIMIIVCYRHMLK 1653

Zfish  1153 --PPLPERSYLMDGMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQP 1215
              |||.|:|.:....:|:    ....||:.....|:.....::..:.:       ||...:...|
  Fly  1654 NAPPLAEQSQIQKESLEN----RANSEAAQRERYYATIHKVSMQNNDK-------IPETSEDISP 1707

Zfish  1216 HMTRRLPCGPVPVPRAPSPPLTQSNPNLS-ANQECPDNTVPHR-SAHKRDLSQGAAQSVENISNT 1278
            :.|.:               |:::..|:| .:...|.||:.|. ..|:|.|::|.:......|..
  Fly  1708 YATFQ---------------LSEAGGNMSQPHHGGPANTLLHSFMYHERALAEGCSSPPPAASKN 1757

Zfish  1279 TPGRAR---PSADHTSSGQKSKVKKKASKSAQYRRDI------------LQGDLPPPPEPPPEDQ 1328
            ....:|   |.::.:.|.|......:...|.|:...|            ...||.|..|
  Fly  1758 RRRHSRKTEPESEESESDQDQLTSSRTESSNQHEGKIKHSIIYHGAQSSTSSDLSPMSE------ 1816

Zfish  1329 DNCAHVGGFECGQTSLERAGSSNSSMERRE---QSTPNRKNSAHRRIDADDDLIPYNKPNFLSRG 1390
                        |.||.|.|.|....::.:   .:||...||              ||.|    .
  Fly  1817 ------------QKSLPRRGRSRYHHQQYQFSTNTTPRHHNS--------------NKMN----N 1851

Zfish  1391 QMSSNCSTTGSSSS 1404
            ..:||.:||.::::
  Fly  1852 NTTSNTNTTATNTT 1865

Known Domains:

Indicated by green bases in alignment.

Software error:

Illegal division by zero at /www/www.flyrnai.org/docroot/cgi-bin/DRSC_prot_align.pl line 592.

For help, please send mail to the webmaster (ritg@hms.harvard.edu), giving this error message and the time and date of the error.