DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo3 and Dscam1

DIOPT Version :9

Sequence 1:	XP_005172693.1	Gene:	robo3 / 30770	ZFINID:	ZDB-GENE-000209-4	Length:	1424	Species:	Danio rerio
Sequence 2:	NP_001246162.1	Gene:	Dscam1 / 35652	FlyBaseID:	FBgn0033159	Length:	2038	Species:	Drosophila melanogaster

Alignment Length:	1758	Identity:	377/1758 - (21%)
Similarity:	599/1758 - (34%)	Gaps:	554/1758 - (31%)

- Green bases have known domain annotations that are detailed below.

Zfish    69 PSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGSLFFLRIVHGRRS 133
            |....|..|.||...|:..|.|...|.|.|||:.:  ...:|             .|||...:
  Fly   353 PPTQTVDFGRPAVFTCQYTGNPIKTVSWMKDGKAI--GHSEP-------------VLRIESVK-- 400

Zfish   134 KPDEGVYVCVARNYLGEAVSRNASLEVAI-LRDD---FRQTPSDVVVAAGEPAVMECIPPRGHPE 194
            |.|:|:|.|..||   :..|..||.|:.: .|.|   .||...:..:..|....::|: ..|:|.
  Fly   401 KEDKGMYQCFVRN---DQESAEASAELKLGGRFDPPVIRQAFQEETMEPGPSVFLKCV-AGGNPT 461

Zfish   195 PTISWKRNNVKVNDKD-----ERITIRG---GKLMISNTRKSDAGMYVCVGTNMVGEKDSDPAEL 251
            |.|||:.:..|:.:.|     :.:|:.|   ..|.|::...:|.|:|.|:..:.||..:.. |:|
  Fly   462 PEISWELDGKKIANNDRYQVGQYVTVNGDVVSYLNITSVHANDGGLYKCIAKSKVGVAEHS-AKL 525

Zfish   252 VVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGRFE-IRADNSLRLTQV 315
            .|:..| ::|:...:.::|.:|:...|.|.|.|..:|.|:::...||..|.: :..:.:|.:..|
  Fly   526 NVYGLP-YIRQMEKKAIVAGETLIVTCPVAGYPIDSIVWERDNRALPINRKQKVFPNGTLIIENV 589

Zfish   316 -KAEDEGSYTCLSENSVGKAEASGSLQVHVGPSLPPQIVVRPRDQISAQGRTVTFLCG-TKGNPP 378
             :..|:.:|||:::|..|.: |.|||:|.|  .:.||||....:.:...|.::...|. .||:.|
  Fly   590 ERNSDQATYTCVAKNQEGYS-ARGSLEVQV--MVLPQIVPFAYEDLINMGDSIDLFCQIQKGDRP 651

Zfish   379 PAVFWQ----------------------KEGSQVLLFPSQPPSQSGR-------------FSVSL 408
            ..|.|.                      .|.:.::..||..|:.:||             :||.|
  Fly   652 IKVHWSFERSAGDYGFDQVQPQMRTNRISEKTSMISIPSASPAHTGRDTCIASNKAGTTTYSVDL 716

Zfish   409 S--------------------------------------------------------------GE 411
            :                                                              |.
  Fly   717 TVNVPPRWILEPTDKAFAQGSDAKVECKADGFPKPQVTWKKAVGDTPGEYKDLKKSDNIRVEEGT 781

Zfish   412 LTITDVHSEDSGYYICQAISVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCH 476
            |.:.::...:.|||:|:||:..||.|:..::     .|.:.||...:...|||...|..|.|||.
  Fly   782 LHVDNIQKTNEGYYLCEAINGIGSGLSAVIM-----ISVQAPPEFTEKLRNQTARRGEPAVLQCE 841

Zfish   477 VMGNPLPSIQWERDGQRI-LGIDERISLMEN-------GTLQITALQETDSGAYTCVASSLSGET 533
            ..|.....|.|..:..|: ...|.|.::.|.       .:|.|...:.:||..:||||::..|..
  Fly   842 AKGEKPIGILWNMNNMRLDPKNDNRYTIREEILSTGVMSSLSIKRTERSDSALFTCVATNAFGSD 906

Zfish   534 SWSGVLTVKESGGLSASPVSEPYQLPGPPQKPVVTDVTRNSVTLTW-QPNAHEGGAAVTSYIIEA 597
            ..|..:.|:|              :|..|....|.|.:..||.|:| ||  ::|.:.:..||||
  Fly   907 DASINMIVQE--------------VPEMPYALKVLDKSGRSVQLSWAQP--YDGNSPLDRYIIE- 954

Zfish   598 FSQSAGSTWQTVADFVKLEKHT----VTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSP 658
            |.:|..| |..: |.|.:..||    |..|||.|.|...:.|.||.|.|..|. :..:.|.:.:|
  Fly   955 FKRSRAS-WSEI-DRVIVPGHTTEAQVQKLSPATTYNIRIVAENAIGTSQSSE-AVTIITAEEAP 1016

Zfish   659 TGQGVDHRQVQRELGEMSVQLHEPVLLTASSV------KISWNVDRQSQYIQGYRLLYRPVGGSW 717
            :|:            ..:::: |||..|...|      :..||.:....|: ||:|  .....|:
  Fly  1017 SGK------------PQNIKV-EPVNQTTMRVTWKPPPRTEWNGEILGYYV-GYKL--SNTNSSY 1065

Zfish   718 SQQEVKGAT----ERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNV 778
            ..:.:...|    |.:..:.||...|:|.:.|:.:.....|..|..:...|.|..||.||.....
  Fly  1066 VFETINFITEEGKEHNLELQNLRVYTQYSVVIQAFNKIGAGPLSEEEKQFTAEGTPSQPPSDTAC 1130

Zfish   779 ATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWCLGND---SQTRYNINQTVDGSVLSTVLKGLL 840
            .|:   .|.:|.|.|..||.:..||||:.|||....:|   .:|:.:..:|...   .|||.||.
  Fly  1131 TTL---TSQTIRVGWVSPPLESANGVIKTYKVVYAPSDEWYDETKRHYKKTASS---DTVLHGLK 1189

Zfish   841 PGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITDVVKQPAFIA 905
            ....|.::|.|.|:.|.|..|.|:.                .::|.:|  .|..|||   .|.:.
  Fly  1190 KYTNYTMQVLATTAGGDGVRSVPIH----------------CQTEPDV--PEAPTDV---KALVM 1233

Zfish   906 GIGG--ACW-------VILMGFSVWIYCR----RKKRKELSHYTASFA-------------YTPA 944
            |...  ..|       .|:..::|:....    ..|.:::.||..||.             .|.:
  Fly  1234 GNAAILVSWRPPAQPNGIITQYTVYSKAEGAETETKTQKVPHYQMSFEATELEKNKPYEFWVTAS 1298

Zfish   945 VGFPHG-ESSGLNGRPG-MLGANMGNYPWLADSWPTTNLVHNGKDSVNCCTSKHETPERYYNEAG 1007
            .....| :|..:...|. .:.|.:.::    |...|.....:.|  :.|.......||..:...|
  Fly  1299 TTIGEGQQSKSIVAMPSDQVPAKIASF----DDTFTATFKEDAK--MPCLAVGAPQPEITWKIKG 1357

Zfish  1008 ISNYLNQNEKYSADSN------------------EGPI------------------YSTIDPTSE 1036
            :....|...:...|.:                  |..|                  :.|:..|:.
  Fly  1358 VEFSANDRMRVLPDGSLLIKSVNRQDAGDYSCHAENSIAKDSITHKLIVLAPPQSPHVTLSATTT 1422

Zfish  1037 D---------------LRSFTAQYSQHTTPYASTPIMPFTNQAPCSPEQDGSHWMT-QPGTSNAQ 1085
            |               |..:|..|......:.::.:...:.:........||.:.. ..|.:|..
  Fly  1423 DALTVKLKPHEGDTAPLHGYTLHYKPEFGEWETSEVSVDSQKHNIEGLLCGSRYQVYATGFNNIG 1487

Zfish  1086 YAQPDRSKTENVKQPKQKLMGKP----VKTPSLN-----WMDALPPPPISGDLEQCDEEDDNELI 1141
            ..:.........|..|.||..||    |.:.|::     |.|...|.             .:.::
  Fly  1488 AGEASDILNTRTKGQKPKLPEKPRFIEVSSNSVSLHFKAWKDGGCPM-------------SHFVV 1539

Zfish  1142 QDMGSDEEEWCP----PLPERSYLMDGMVEDGPVPPPRGEASSPTTSY-----SHQST------- 1190
            :....|:.||..    ..|:.:|::..:              .|.|.|     :|.|.
  Fly  1540 ESKKRDQIEWNQISNNVKPDNNYVVLDL--------------EPATWYNLRITAHNSAGFTVAEY 1590

Zfish  1191 --ATLTP-----SPRDDGHFHD---------------------IPRL------------------ 1209
              ||||.     :|.|||..|.                     :|.:
  Fly  1591 DFATLTVTGGTIAPLDDGSGHGNVHTRIRLPAWMPEWLDLNFMVPLIATVVVVAVGICVVCVALS 1655

Zfish  1210 --------------------HQLEQPHMT--RRLP-----CGPVPVPRAPSPPLTQS-------- 1239
                                :|...|..|  :|.|     .|.:..|....||:..|
  Fly  1656 RRRADDMRGGQKDVYYDVVYNQTMGPGATLDKRRPDLRDELGYIAPPNRKLPPVPGSNYNTCDRI 1720

Zfish  1240 ------------------NPNL-------------------SANQEC-------PDNTVPHRSAH 1260
                              ||||                   ..|.||       .|...|:.:.|
  Fly  1721 KRGRGGLRSNHSTWDPRRNPNLYEELKAPPVPMHGNHYGHAHGNAECHYRHPGMEDEICPYATFH 1785

Zfish  1261 ----KRDLSQGAAQSVENI------SNTTPGRA-------RPSADHTSSGQKSKVKKKASKSAQY 1308
                :.::....|.:.:..      :...||.|       .|...|:.||.:|     ..::.:|
  Fly  1786 LLGFREEMDPTKAMNFQTFPHQNGHAGPVPGHAGTMLPPGHPGHVHSRSGSQS-----MPRANRY 1845

Zfish  1309 RRDILQGDLPPPPEPPPE--DQDNCAHVGGFECGQTSLERAGSSNSSMERREQSTPNRKNSAHRR 1371
            :|...||.......|.||  |..|||....:. ..|.:...|.|..|....|...|  .|.|
  Fly  1846 QRKNSQGGQSSIYTPAPEYDDPANCAEEDQYR-RYTRVNSQGGSLYSGPGPEYDDP--ANCA--- 1904

Zfish  1372 IDADDDLI------PYNKP--NFLSRGQMSSNCSTTGSSSSRGSTGS-RGQGTGR-------KRN 1420
              .::|..      ||.:|  ::.|||.|           .|.|.|| |..|.|.       .||
  Fly  1905 --PEEDQYGSQYGGPYGQPYDHYGSRGSM-----------GRRSIGSARNPGNGSPEPPPPPPRN 1956

Zfish  1421 DEM 1423
            .:|
  Fly  1957 HDM 1959

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo3	XP_005172693.1	Ig1_Robo	62..161	CDD:143317	28/91 (31%)
		I-set	63..160	CDD:254352	27/90 (30%)
		I-set	167..253	CDD:254352	24/93 (26%)
		Ig	169..253	CDD:299845	23/91 (25%)
		I-set	257..342	CDD:254352	25/86 (29%)
		Ig3_Robo	274..342	CDD:143202	21/69 (30%)
		IG_like	349..444	CDD:214653	31/192 (16%)
		Ig	364..453	CDD:299845	28/186 (15%)
		IG_like	460..541	CDD:214653	25/88 (28%)
		IGc2	467..528	CDD:197706	20/68 (29%)
		FN3	559..653	CDD:238020	35/98 (36%)
		FN3	685..764	CDD:238020	18/88 (20%)
		FN3	<793..866	CDD:238020	26/75 (35%)
Dscam1	NP_001246162.1	Ig	38..130	CDD:299845
		IG	57..133	CDD:214652
		IG_like	267..343	CDD:214653
		Ig	269..340	CDD:143165
		IG_like	353..425	CDD:214653	28/91 (31%)
		IGc2	361..413	CDD:197706	20/68 (29%)
		I-set	433..527	CDD:254352	24/95 (25%)
		IGc2	446..517	CDD:197706	18/71 (25%)
		I-set	533..618	CDD:254352	25/85 (29%)
		IGc2	544..607	CDD:197706	17/62 (27%)
		Ig	641..714	CDD:143165	12/72 (17%)
		IGc2	735..804	CDD:197706	8/68 (12%)
		I-set	819..914	CDD:254352	26/94 (28%)
		Ig	833..921	CDD:299845	25/101 (25%)
		FN3	918..1011	CDD:238020	35/98 (36%)
		FN3	1018..1116	CDD:238020	22/113 (19%)
		FN3	1124..1217	CDD:238020	32/114 (28%)
		FN3	1222..1312	CDD:238020	17/92 (18%)
		Ig	1339..1406	CDD:299845	8/66 (12%)
		FN3	1409..1499	CDD:238020	10/89 (11%)
		FN3	1504..1584	CDD:238020	19/106 (18%)
		Dscam_C	1890..2008	CDD:289151	23/88 (26%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10075
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	3.010