Protein Alignment ephb4b and Eph

DIOPT Version :10

• Sequence 1: NP_571492.1
• Gene: ephb4b / 30691
• ZFINID: ZDB-GENE-990415-65
• Length: 976
• Species: Danio rerio
• Sequence 2: NP_524625.3
• Gene: Eph / 43803
• FlyBaseID: FBgn0025936
• Length: 1096
• Species: Drosophila melanogaster
• Alignment Length: 1055
• Identity: 415/1055 - (39%)
• Similarity: 599/1055 - (56%)
• Gaps: 148/1055 - (14%)

Zfish    20 LVSAEEEVLMNTKLETSDLRWTIYPSG----DPEWEEMSGLD-EEGNSVRTFQVCPMD-SSVSHW 78
            |..|::.||::|..|.: |.||.||.|    .|.|.|.|..| .:|.:.|::.||.:. .:|::|
  Fly    79 LAHADQVVLLDTTREAT-LEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNNW 142

Zfish    79 LRTRFIPRHGASQVYVEIRFTMMECSAMPASFRTCKETFNLYYYQSDEDTASATHPAWMENPYSK 143
            |.:.||.|..|:::|:||:||:.:||..|.:..:|||||:|.:|:.  |.|:...|.|..:.|..
  Fly   143 LWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEF--DAATREPPPWQTDSYRL 205

Zfish   144 VDTVAADFLLRRGGERKSN----------VKTVRVGPLSLFGFYLAFQTQGACMALLSVRVFFKK 198
            :..:||       ||.:.|          ||::.|...   |.|.||:.||||:::|:|:|::..
  Fly   206 IARIAA-------GEGRFNQNSDVDINTEVKSIAVNKK---GVYFAFRDQGACISVLAVKVYYIT 260

Zfish   199 CPAVSRAFSSFPETLPH----SLVQQAEGVCVDNSAPTGQCTAPPTMFCGEDGQWVGPPSSTCAC 259
            ||||:..|:.|.|| |.    :::::..|.||||:.|    ...||..|..||:|. ..:..|.|
  Fly   261 CPAVTENFAHFNET-PTGREITIIEKQNGTCVDNAEP----YETPTYLCKGDGKWT-ILTGGCRC 319

Zfish   260 KPGYEPVDSDR-CRACGLGQYKASVGGSLCRVCPDNSNTHSAGSSLCVCRPGYHRATSDLPDSAC 323
            |.||||..::: |..|.||.:| |...:.|..||.|||....||..|.|..|::|..:|.....|
  Fly   320 KAGYEPNYTNKTCTECPLGTFK-SPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPC 383

Zfish   324 TKPPSAPRSI-IYQINDTVVTLEWSEPLDRGG----------RSDLSYSVECMHCRGSLCVQCAD 377
            ..||:||.:: :..::.|...:.||.|.....          .||:.|.::|.        .|:.
  Fly   384 YSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCN--------ICSP 440

Zfish   378 SITYRPGQMGVSGRRVIIRGLLPHTTYTFTVLAQNGVSAVS----HTSPAS------------SS 426
            ::.|.|.....:..::.:..|.|.||||..:.|.|.||.::    |::.:|            |.
  Fly   441 NVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSL 505

Zfish   427 VNI----------------TTSRDVAVPVSGIRRIKASESSVSISWTVPPQTQHSIQDYQLRYSL 475
            :||                ||...:...|..:|.:..:.....:.|..|.|:...::.|::|:..
  Fly   506 LNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFP 570

Zfish   476 KGQDD--GWQYVSSRSSSVVLNDLSRASQYQVQVRARTAAGYGHFSSAV------SISTLPDDEE 532
            |.:.|  ....::::.:...:..|...::|..|||.:|..|:|.:|:.:      |:.::.||
  Fly   571 KVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDD-- 633

Zfish   533 SPSRLMLTGVLVAIGLLILIAVVIVAVFCFRRSTRRRDPDPDKSGQF------------------ 579
            |.....:.|.:|. |:|.|:..:|..|: |.||..:.|.|...:...
  Fly   634 SVQIRFIAGAIVT-GVLFLVIFIIATVY-FMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMDTTP 696

Zfish   580 ------------LMGQGIKVYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVCRGRL 632
                        |.|.. :.|:||.||||||:|:||||:|||.:::.||.:||.||||:||||||
  Fly   697 IVKKLHLNVTTPLFGNS-RSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRL 760

Zfish   633 KVPGKKENY-----VAIKTLKGGYTDKQRRDFLSEASIMGQFQHPNIIHLEGVITASCPVMILTE 692
            |:|   .|:     |||||||.|.::|.|.|||:||||||||.|||:|:|:||:|.|.||||:||
  Fly   761 KIP---PNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITE 822

Zfish   693 YMENGALDSFLRLNDGQFTPIQLVGMLRGIASGMKYLSEMSFVHRDLAARNILVNSNLVCKVSDF 757
            |||||:||:|||:|||:|..:||:.|||||||||.|||:|::|||||||||:|||:.|:||::||
  Fly   823 YMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADF 887

Zfish   758 GLSRFLTENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDVWSYGIVMWEVMSFGERPYWDM 822
            ||||.: ||:|| .||:. |||||:|||||||||||||||||||||||:|:|||||:||||||:.
  Fly   888 GLSREI-ENASD-AYTTR-GGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNW 949

Zfish   823 SNQDVINAIEQDYRLPPPPECPASLHQLMLDCWQKERSSRPRFCAIVSALDRLIRNPASLKITGR 887
            ||||||.:||:.||||.|.:||.:|:|||||||||:|:.||.|.:|||.||.|.|.|.||..|..
  Fly   950 SNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRP 1014

Zfish   888 IPDGPSHPLLDQRAPPPLSHCSSVADWLRAIKMERYEDAFMQAGFTAIQHITHISTEDLLRIGVT 952
            .|:...:.:||.:....:...:.:  ||..|||.||...|.:|.....|.|:.::.:.|..:|:|
  Fly  1015 SPESDGNHILDGQRGQNIFISTDL--WLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGIT 1077

Zfish   953 LAGHQKKILSSVQTL 967
            |.|||||||...:.|
  Fly  1078 LVGHQKKILHQARQL 1092

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ephb4b	NP_571492.1	EphR_LBD	25..198	CDD:470656	65/188 (35%)
		FN3	327..431	CDD:238020	29/146 (20%)
		FN3	438..526	CDD:238020	19/95 (20%)
		EphA2_TM	540..610	CDD:464211	30/99 (30%)
		PTKc_EphR_B	608..876	CDD:173638	185/272 (68%)
		SAM_superfamily	906..969	CDD:472832	23/62 (37%)
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	65/187 (35%)
		FN3	387..476	CDD:238020	21/96 (22%)
		FN3	536..623	CDD:238020	17/86 (20%)
		EphA2_TM	640..738	CDD:464211	30/100 (30%)
		PTKc_EphR	736..1002	CDD:270629	185/271 (68%)
		SAM_1	1029..1092	CDD:425739	22/64 (34%)