DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ephb4b and Eph

DIOPT Version :9

Sequence 1:	NP_571492.1	Gene:	ephb4b / 30691	ZFINID:	ZDB-GENE-990415-65	Length:	976	Species:	Danio rerio
Sequence 2:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster

Alignment Length:	1055	Identity:	415/1055 - (39%)
Similarity:	599/1055 - (56%)	Gaps:	148/1055 - (14%)

- Green bases have known domain annotations that are detailed below.

Zfish    20 LVSAEEEVLMNTKLETSDLRWTIYPSG----DPEWEEMSGLD-EEGNSVRTFQVCPMD-SSVSHW 78
            |..|::.||::|..|.: |.||.||.|    .|.|.|.|..| .:|.:.|::.||.:. .:|::|
  Fly    79 LAHADQVVLLDTTREAT-LEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNNW 142

Zfish    79 LRTRFIPRHGASQVYVEIRFTMMECSAMPASFRTCKETFNLYYYQSDEDTASATHPAWMENPYSK 143
            |.:.||.|..|:::|:||:||:.:||..|.:..:|||||:|.:|:.  |.|:...|.|..:.|..
  Fly   143 LWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEF--DAATREPPPWQTDSYRL 205

Zfish   144 VDTVAADFLLRRGGERKSN----------VKTVRVGPLSLFGFYLAFQTQGACMALLSVRVFFKK 198
            :..:||       ||.:.|          ||::.|...   |.|.||:.||||:::|:|:|::..
  Fly   206 IARIAA-------GEGRFNQNSDVDINTEVKSIAVNKK---GVYFAFRDQGACISVLAVKVYYIT 260

Zfish   199 CPAVSRAFSSFPETLPH----SLVQQAEGVCVDNSAPTGQCTAPPTMFCGEDGQWVGPPSSTCAC 259
            ||||:..|:.|.|| |.    :::::..|.||||:.|    ...||..|..||:|. ..:..|.|
  Fly   261 CPAVTENFAHFNET-PTGREITIIEKQNGTCVDNAEP----YETPTYLCKGDGKWT-ILTGGCRC 319

Zfish   260 KPGYEPVDSDR-CRACGLGQYKASVGGSLCRVCPDNSNTHSAGSSLCVCRPGYHRATSDLPDSAC 323
            |.||||..::: |..|.||.:| |...:.|..||.|||....||..|.|..|::|..:|.....|
  Fly   320 KAGYEPNYTNKTCTECPLGTFK-SPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPC 383

Zfish   324 TKPPSAPRSI-IYQINDTVVTLEWSEPLDRGG----------RSDLSYSVECMHCRGSLCVQCAD 377
            ..||:||.:: :..::.|...:.||.|.....          .||:.|.::|.        .|:.
  Fly   384 YSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCN--------ICSP 440

Zfish   378 SITYRPGQMGVSGRRVIIRGLLPHTTYTFTVLAQNGVSAVS----HTSPAS------------SS 426
            ::.|.|.....:..::.:..|.|.||||..:.|.|.||.::    |::.:|            |.
  Fly   441 NVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSL 505

Zfish   427 VNI----------------TTSRDVAVPVSGIRRIKASESSVSISWTVPPQTQHSIQDYQLRYSL 475
            :||                ||...:...|..:|.:..:.....:.|..|.|:...::.|::|:..
  Fly   506 LNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFP 570

Zfish   476 KGQDD--GWQYVSSRSSSVVLNDLSRASQYQVQVRARTAAGYGHFSSAV------SISTLPDDEE 532
            |.:.|  ....::::.:...:..|...::|..|||.:|..|:|.:|:.:      |:.::.||
  Fly   571 KVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDD-- 633

Zfish   533 SPSRLMLTGVLVAIGLLILIAVVIVAVFCFRRSTRRRDPDPDKSGQF------------------ 579
            |.....:.|.:|. |:|.|:..:|..|: |.||..:.|.|...:...
  Fly   634 SVQIRFIAGAIVT-GVLFLVIFIIATVY-FMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMDTTP 696

Zfish   580 ------------LMGQGIKVYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVCRGRL 632
                        |.|.. :.|:||.||||||:|:||||:|||.:::.||.:||.||||:||||||
  Fly   697 IVKKLHLNVTTPLFGNS-RSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRL 760

Zfish   633 KVPGKKENY-----VAIKTLKGGYTDKQRRDFLSEASIMGQFQHPNIIHLEGVITASCPVMILTE 692
            |:|   .|:     |||||||.|.::|.|.|||:||||||||.|||:|:|:||:|.|.||||:||
  Fly   761 KIP---PNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITE 822

Zfish   693 YMENGALDSFLRLNDGQFTPIQLVGMLRGIASGMKYLSEMSFVHRDLAARNILVNSNLVCKVSDF 757
            |||||:||:|||:|||:|..:||:.|||||||||.|||:|::|||||||||:|||:.|:||::||
  Fly   823 YMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADF 887

Zfish   758 GLSRFLTENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDVWSYGIVMWEVMSFGERPYWDM 822
            ||||.: ||:|| .||:. |||||:|||||||||||||||||||||||:|:|||||:||||||:.
  Fly   888 GLSREI-ENASD-AYTTR-GGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNW 949

Zfish   823 SNQDVINAIEQDYRLPPPPECPASLHQLMLDCWQKERSSRPRFCAIVSALDRLIRNPASLKITGR 887
            ||||||.:||:.||||.|.:||.:|:|||||||||:|:.||.|.:|||.||.|.|.|.||..|..
  Fly   950 SNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRP 1014

Zfish   888 IPDGPSHPLLDQRAPPPLSHCSSVADWLRAIKMERYEDAFMQAGFTAIQHITHISTEDLLRIGVT 952
            .|:...:.:||.:....:...:.:  ||..|||.||...|.:|.....|.|:.::.:.|..:|:|
  Fly  1015 SPESDGNHILDGQRGQNIFISTDL--WLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGIT 1077

Zfish   953 LAGHQKKILSSVQTL 967
            |.|||||||...:.|
  Fly  1078 LVGHQKKILHQARQL 1092

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ephb4b	NP_571492.1	EphR_LBD	25..198	CDD:383093	65/188 (35%)
		FN3	327..431	CDD:238020	29/146 (20%)
		FN3	438..526	CDD:238020	19/95 (20%)
		EphA2_TM	544..608	CDD:373140	27/93 (29%)
		PTKc_EphR_B	608..876	CDD:173638	185/272 (68%)
		SAM_superfamily	906..969	CDD:387673	23/62 (37%)
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	65/187 (35%)
		FN3	387..476	CDD:238020	21/96 (22%)
		FN3	536..623	CDD:238020	17/86 (20%)
		EphA2_TM	644..736	CDD:291255	27/94 (29%)
		PTKc_EphR	736..1002	CDD:270629	185/271 (68%)
		Pkinase_Tyr	741..999	CDD:285015	181/263 (69%)
		SAM_1	1033..1092	CDD:278937	22/60 (37%)
		SAM	1039..1092	CDD:197735	22/52 (42%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	396	1.000	Domainoid score	I723
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	753	1.000	Inparanoid score	I540
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm24639
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	12	11.740