Protein Alignment GSPT1 and eRF3

DIOPT Version :9

• Sequence 1: NP_002085.3
• Gene: GSPT1 / 2935
• HGNCID: 4621
• Length: 637
• Species: Homo sapiens
• Sequence 2: NP_001260415.1
• Gene: eRF3 / 34658
• FlyBaseID: FBgn0020443
• Length: 619
• Species: Drosophila melanogaster
• Alignment Length: 642
• Identity: 375/642 - (58%)
• Similarity: 441/642 - (68%)
• Gaps: 89/642 - (13%)

Human    56 AQREN--LSAAFSRQLNVNAKPFVPN------VHAAEFVPSFL-----------RGP-------- 93
            |.:||  :|..|| .|||||..|||:      |:.||...:.:           .||
  Fly     2 AAQENTEISTKFS-TLNVNAVEFVPSFSYNSVVNVAEEAAAVVVAAAAAEETADPGPASGSATPA 65

Human    94 --------------AAPPPPVGGAANNHGAGSGAGGRAAPVESSQEEQSLCEGS-----NSAVSM 139
                          ||||||..     .|||:.|.. |.|..||....|...||     ::..:.
  Fly    66 TTPDSVGSGGSTAGAAPPPPTA-----QGAGAAAPS-AQPPTSSSNSASPAPGSPATTPSAGAAA 124

Human   140 ELSEPI--------VENGETEMSPEESWE--------HKEEISEAEPGGGSLGDGRPPEESAHEM 188
            ....||        :.|.||:  |.:||:        ..||:.:||...|
  Fly   125 ADPTPIDGLNPSDKIANNETD--PADSWDVDDAVITPEDEEVEDAEFTEG--------------- 172

Human   189 MEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKE 253
             |...::.|.|.|.......|:|||||||||||||||||||||||.|||||||||||||||||:|
  Fly   173 -EATPKVSKKKVVKVEENRSKREHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKYEREARE 236

Human   254 KNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAV 318
            |:||:|||||||||||||||||||||||||:|||::||||||||||||||||||||||:||||||
  Fly   237 KSRESWYLSWALDTNQEERDKGKTVEVGRAFFETDRKHFTILDAPGHKSFVPNMIGGAAQADLAV 301

Human   319 LVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFL 383
            ||||||||||||||::|||||||||||||||||||:||:||||||||||...||.|||:|::|:|
  Fly   302 LVISARKGEFETGFDRGGQTREHAMLAKTAGVKHLVVLVNKMDDPTVNWDQTRYNECKDKILPYL 366

Human   384 KKVGFNPKKDIHFMPCSGLTGANLKEQ--SDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVD 446
            ||:||||.||:.|||||||:|..||:|  ...||||.|..|||::|.||:.||..|||..:||||
  Fly   367 KKLGFNPAKDLTFMPCSGLSGYGLKDQIPETLCPWYRGPAFIPFIDELPSLNRKSDGPFIMPIVD 431

Human   447 KYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEE 511
            |||||||||:||:|||:..|||.|::|||:..|.|..:.|||.|..:|.||||:||:|||||||:
  Fly   432 KYKDMGTVVMGKVESGTARKGQNLLVMPNRTQVAVDQLFSDDFEVTSVGPGENVKIKLKGIEEED 496

Human   512 ILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGE 576
            :.|||:|||..|...:|:.||||:||:|||||||.||:||:|||...|||.:.|||||||||||:
  Fly   497 VSPGFVLCDAANPIKTGKIFDAQVVILEHKSIICAGYSAVMHIHCAAEEVTVKALICLVDKKSGD 561

Human   577 KSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLV 633
            ||||||||||||||.|.|:..:|.||||.||.||||||||||||.||||||||||:|
  Fly   562 KSKTRPRFVKQDQVAIMRIECSGMICLEQFKLFPQMGRFTLRDENKTIAIGKVLKVV 618

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
GSPT1	NP_002085.3	PAM2	64..81	CDD:284542	10/22 (45%)
		TEF1	210..634	CDD:227581	321/426 (75%)
eRF3	NP_001260415.1	PAM2	12..28	CDD:284542	10/16 (63%)
		TEF1	191..619	CDD:227581	322/428 (75%)
		EF1_alpha	197..416	CDD:206670	179/218 (82%)
		eRF3_II	424..505	CDD:293906	50/80 (63%)
		eRF3_C_III	511..617	CDD:294003	79/105 (75%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	365	1.000	Domainoid score	I935
eggNOG	1	0.900	-	-		E1_COG5256
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H68226
Inparanoid	1	1.050	688	1.000	Inparanoid score	I702
Isobase	1	0.950	-	0	Normalized mean entropy	S365
OMA	1	1.010	-	-		QHG54149
OrthoDB	1	1.010	-	-		D1150082at2759
OrthoFinder	1	1.000	-	-		FOG0002347
OrthoInspector	1	1.000	-	-		otm40644
orthoMCL	1	0.900	-	-		OOG6_101550
Panther	1	1.100	-	-	LDO	PTHR23115
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2104
SonicParanoid	1	1.000	-	-		X149
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	16	15.820