DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Adcy8 and Ac78C

DIOPT Version :9

Sequence 1:	NP_058838.1	Gene:	Adcy8 / 29241	RGDID:	2036	Length:	1248	Species:	Rattus norvegicus
Sequence 2:	NP_001262138.1	Gene:	Ac78C / 40333	FlyBaseID:	FBgn0024150	Length:	1727	Species:	Drosophila melanogaster

Alignment Length:	1524	Identity:	491/1524 - (32%)
Similarity:	725/1524 - (47%)	Gaps:	413/1524 - (27%)

- Green bases have known domain annotations that are detailed below.

  Rat    59 GGGSRKASNPAGSGPNHHAPQL--SSDSVLPLYSLGSGERAHNTGGTKVFPERSGSG-------- 113
            |.|::...|.:|..|    |.|  |:.|:|      |..|....||    |.|.|..
  Fly   182 GDGAQMEDNGSGDLP----PSLRASTTSIL------SNLRKKQQGG----PLRGGVAKQTPLRVQ 232

  Rat   114 -----SASGSGGG----------GDLGFLH-------LDCAPSNSDFFLN--------GGYSYRG 148
                 .|..:.|.          |:..|.|       |...|..|...||        .|...:|
  Fly   233 SVRIVEAKRAYGPKRRTESCSIFGNSNFEHDLESGNSLASIPGQSQRPLNNEMYSAFKSGNVVKG 297

  Rat   149 VIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDPLK---- 209
            ::.|:|.||||...|||.|..|...||:||.:::||:|.:.|:.|..:.: :..:.:.|::
  Fly   298 ILCPSLTNSFKQSSLERSYLTYTHRQRQKSLIIVNVVDFVLKIVLAFVWI-MRRSELGPIESDDG 361

  Rat   210 -----GILLGFFTGI-EVVICALVVVRKDTTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGDGI 268
                 .|......|| .:.||.|...|  ..::.||.::.|.|||....|        |.:|.|:
  Fly   362 TSMATAITWSVCCGIANMAICFLGYWR--CFANNYLHWAAVCTWVLFNIQ--------GFVGQGV 416

  Rat   269 G---------YVLFTLFATYSMLPLPLTWAILAGLGTSLLQVTLQVLI----------PRLAVFS 314
            |         |:||.:|..|:||||||.|.::.|..|:...:.:..:|          |...:|
  Fly   417 GFADREYLVWYILFVIFVPYAMLPLPLKWCVVGGTITASCHLAVITIIKLQHGEATINPECVLF- 480

  Rat   315 INQVLAQVVLFMCMNTAGIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLPRFV 379
              |:.|..:|:..:|.||::..||:||.||.||:||.:.:|.:...|.|.||.::|:.|:||..|
  Fly   481 --QIFANFILYTAINVAGMYTKYLTDRGQRLAFIETHKAMEHKKESEKELQRTQKLLDSILPNIV 543

  Rat   380 VLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELFARFD 444
            ..::.::|....|..::.||:::|::..:||||||||:||||.|::..|||:||::||:||||||
  Fly   544 NNQIRSEMYKGTDPTVETQFNKLYVYPMDNVSILFADIKGFTELASKTSAQQLVKILNDLFARFD 608

  Rat   445 RLAHEHHCLRIKILGDCYYCVSGLP----EPRQDHAHCCVEMGLSMIKTIRFVRSRTKHDVDMRI 505
            |:|.::||||:|:|||||||||...    :.|.|||.|.||.||.|||.|:.||..|..|::|||
  Fly   609 RIAEDNHCLRVKLLGDCYYCVSQFESDNWKTRPDHAVCSVETGLHMIKAIKDVRLHTHVDLNMRI 673

  Rat   506 GIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLSGDYNVEEGHGKER 570
            |||||||:|||||.:||.|||||.||.|||.:||||||||:|||:|||.||:..|.||.|:|..|
  Fly   674 GIHSGSVMCGVLGNKKWHFDVWSNDVIIANHMESGGIPGRVHISEATLKCLNDAYEVEPGNGGCR 738

  Rat   571 NEFLRKHNIETYLIKQPEE---------------------------------------------- 589
            :..|:..|::|||||:.|.
  Fly   739 DNHLKMLNVKTYLIKRTEPLRPKRRFGTRSSAHLAGSVATAAPPCATPTALPKSISASGSGSIGG 803

  Rat   590 -----------------------------------------SLLSLPEDIVKESVSCSDRR---- 609
                                                     ||.|||.  |.|.|:...||
  Fly   804 VTATGNDGASIDGRSLEYAATIAVPAQQPTQLLQQQQKKNISLNSLPN--VVEGVAMDSRRIRNG 866

  Rat   610 ---NSGATFTEG----------------------------------------------------- 618
               ..||..|.|
  Fly   867 CGVMGGAGGTGGGGTGPCGMSTVSSPTAMMSAPLEVQLRPRNGACSIQNLAEAIDGKGLIIEDES 931

  Rat   619 --SWSPELPFDNIVGKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDKLR 681
              .|.||:||.|:...::   .|.|....|:.......:.|....|||::.||..|.:.|..::|
  Fly   932 TTDWIPEIPFKNLNSPED---GLNRADSILVDTKEDHRVSVAVLDEEIDEFIEQNIQINSNKEIR 993

  Rat   682 REHIKPFSLMFKDSSLEHKYSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRLMPMTIQFSILI 746
            ||::.|::|.|||.|.|.|:.|:|:::|:||::|.|::.:|:...|.:: ..|...:.|..|:..
  Fly   994 REYLNPWTLKFKDKSQEQKFCQLREDMFRSNMLCVFVIWIFMVLCQVII-IPRCTRLIICLSVGT 1057

  Rat   747 MLHSALVLITTAEDYKCLPLIL------------RKT---C------CWI---------NETYLA 781
            ::.:...::..||::..||..|            |:|   |      |.:         :.||:.
  Fly  1058 VILTFCCVLVMAEEFPGLPRCLKTNSAKLVHQRQRRTLFICGVIVSMCMLSAIGLVLCPSSTYIG 1122

  Rat   782 R--------------------------NVIIFASILIN-------------------------FL 795
            .                          ||.:.|:|..|                         |:
  Fly  1123 TADEHSRFLRMVSPYSNISMTEKEFKLNVYLSATIHHNITISTPASGGPTPLVDITSSGPSDEFV 1187

  Rat   796 GAVINILWCDFDKSIP----------------LKNLTFNSSAVF---TDI---CSYPEYFVFTGV 838
            .:.:|.|..:....:|                |.||:::.::|.   :|:   |::|||.|||.|
  Fly  1188 RSTLNALTLNLTPPLPQSHRPYTLTTNGHSVSLSNLSYSDASVMQSSSDVEGQCAHPEYLVFTWV 1252

  Rat   839 LAMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETIYAGLFLSYDNLNHSGEDF-----------L 892
            |.:|:.|..|:|..::|..:.|.|:|.|..|       :.:.:.    ||:.|           |
  Fly  1253 LCLVSLATALKLYYLVKALMALAMVAFYTTL-------IMMKFG----SGDSFSLVELSRLGMPL 1306

  Rat   893 GTKEASLLLMAMFLLAVFYHGQQLEYTARLDFLWRVQAKEEINEMKDLREHNENMLRNILPGHVA 957
            |.:  .|:|:..||:.|.||.:.:|.|:||||:|:.||:.|:..||..|..|:.:::||||.|||
  Fly  1307 GVQ--MLILLISFLVMVCYHARLVEVTSRLDFIWKEQAERELTNMKSNRALNDTLIKNILPDHVA 1369

  Rat   958 RHFLEKDRDNEELYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGED 1022
            .::| .|...:||||:.::..||||||||.|.||||: :::| |..|:|:|||||.||||||.|.
  Fly  1370 TYYL-SDEHTDELYSKMHNLCGVMFASIPNFQDFYSE-DIDN-GKACIRILNEIICDFDELLEEP 1431

  Rat  1023 RFQDIEKIKTIGSTYMAVSGLSPEK-----QQCEDKWGHLCALADFSLALTESIQEINKHSFNNF 1082
            ||..:|||||:|:||||.:||:.|.     :..||.   :|.|.:|:.|:.:.::|||..:||||
  Fly  1432 RFASVEKIKTVGATYMAAAGLNHEHLRLRGETSEDS---VCDLVEFAFAMKQKLEEINGDAFNNF 1493

  Rat  1083 ELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFAFDYR 1147
            :||:||..|.:|:|||||:||.|||||.|||:||||||||.:.|:||||.|..:|..:|:....|
  Fly  1494 QLRVGICSGPLVSGVIGARKPVYDIWGNTVNVASRMDSTGENWRVQVPENTAELLCSRGYTCVKR 1558

  Rat  1148 GEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQYSLAAVVLGLVQSLNRQRQKQLLNEN 1212
            |||.|||    :|.:.|:::..:......::.|.|:|....||       |:.|.|||..  :..
  Fly  1559 GEIAVKG----KGMMTTFYVHPKGISESQLISPVRMPAGIPLA-------QTPNLQRQTS--HHG 1610

  Rat  1213 SNSGII---KSHYNRRTLL--TPSG-PEP 1235
            |.|.::   .....|.|.:  ||:| |.|
  Fly  1611 SFSAVVFGMLQASKRSTAIAGTPTGTPSP 1639

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Adcy8	NP_058838.1	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000269\|PubMed:16258073, ECO:0000269\|PubMed:22494970, ECO:0000269\|PubMed:22976297	1..179		43/159 (27%)
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000269\|PubMed:22976297	1..106		14/48 (29%)
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	38..40
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	49..51
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	51..117		18/72 (25%)
		AC_N	<158..397	CDD:292831	80/267 (30%)
		CYCc	363..561	CDD:214485	115/201 (57%)
		Guanylate_cyc	402..586	CDD:278633	115/187 (61%)
		DUF1053	614..708	CDD:283888	32/148 (22%)
		CYCc	941..1145	CDD:214485	109/208 (52%)
		Guanylate_cyc	970..1169	CDD:278633	106/203 (52%)
		Involved in CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	1106..1248		51/136 (38%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000269\|PubMed:19305019	1197..1212		5/14 (36%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1220..1248		7/19 (37%)
Ac78C	NP_001262138.1	AC_N	<288..551	CDD:292831	84/276 (30%)
		CYCc	526..729	CDD:214485	115/202 (57%)
		Guanylate_cyc	566..753	CDD:278633	114/186 (61%)
		DUF1053	929..1020	CDD:283888	30/93 (32%)
		CYCc	1353..1554	CDD:214485	109/206 (53%)
		Guanylate_cyc	1381..1576	CDD:278633	106/203 (52%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	133	1.000	Domainoid score	I4939
eggNOG	1	0.900	-	-		E1_COG2114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H37443
Inparanoid	1	1.050	758	1.000	Inparanoid score	I525
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D107368at2759
OrthoFinder	1	1.000	-	-		FOG0006020
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100296
Panther	1	1.100	-	-	LDO	PTHR45627
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X4318
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	12	11.830