Protein Alignment GRK5 and Gprk2

DIOPT Version :10

• Sequence 1: NP_005299.1
• Gene: GRK5 / 2869
• HGNCID: 4544
• Length: 590
• Species: Homo sapiens
• Sequence 2: NP_476867.1
• Gene: Gprk2 / 49045
• FlyBaseID: FBgn0261988
• Length: 714
• Species: Drosophila melanogaster
• Alignment Length: 719
• Identity: 381/719 - (52%)
• Similarity: 459/719 - (63%)
• Gaps: 134/719 - (18%)

Human     1 MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCEDLRRTIDRDYCSLCDKQPIGRL 65
            |||||||||||.|||||||....|||||||::||:|||||||.:|:..:|..|..:.|:|||||.
  Fly     1 MELENIVANTVYLKAREGGSDSNKGKSKKWRKILQFPHISQCINLKDKLDISYGYVIDQQPIGRE 65

Human    66 LFRQFCET-RPGLECYIQFLDSVAEYEV------------------------------------- 92
            |||.|||. ||....||.|||.|.:||:
  Fly    66 LFRLFCENKRPVYFRYITFLDEVVKYEIEYISNRIFIGHDIGRRFLDVEAQLELRNGSGGDALDA 130

Human    93 -TPDEKL---------------------------------GEKGKEIMTKYLTPKS--------- 114
             |.:|.|                                 .:...:|..|.|..::
  Fly   131 ETQEELLLNSSNANPTETAETEHCNNTTANNCNNINNSNNSQHSSDINHKKLDTRNHNGDDATGN 195

Human   115 ------------------------------------PV-------FIAQVGQDLVSQTEEKLLQK 136
                                                ||       .:..:..||::|...| |..
  Fly   196 GSSHQDDGDESVKCQEGHDDAEKGGGGEGGGGGKCVPVGGYPDELVLDVLNDDLIAQVRNK-LNS 259

Human   137 PCKELFSACAQSVHEYLRGEPFHEYLDSMFFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVC 201
            ..|::|:.|..:|..:|.||||.|:..||:|.|:|||||||.||:|..|||.|||||||||||||
  Fly   260 GGKDIFAQCVNAVKAFLAGEPFREFESSMYFHRYLQWKWLEAQPITYKTFRMYRVLGKGGFGEVC 324

Human   202 ACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIM 266
            ||||||||||||||:||||||||||||||.|.|||||:|:||.||||||||||||||||||||||
  Fly   325 ACQVRATGKMYACKKLEKKRIKKRKGESMVLIEKQILQKINSPFVVNLAYAYETKDALCLVLTIM 389

Human   267 NGGDLKFHIYNM-GNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDL 330
            |||||||||||| |.||||.|||.|||||:.|||:.||::..||||.|||||||||:||:|||||
  Fly   390 NGGDLKFHIYNMGGEPGFELERARFYAAEVACGLQHLHKQGIVYRDCKPENILLDDHGHVRISDL 454

Human   331 GLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKRE 395
            ||||:||||:::||||||||||||||::|::|..|||::..|||:|||||||:|||.||||||||
  Fly   455 GLAVEIPEGEMVRGRVGTVGYMAPEVIDNEKYAFSPDWFSFGCLLYEMIEGQAPFRMRKEKVKRE 519

Human   396 EVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQ--EEGAAEVKRHPFFRN--MNFKRL 456
            ||||||.|..|.||.||::||||:|:.||.|..||||||:  ..|..:|..||||.:  :|::||
  Fly   520 EVDRRVKEDPEKYSSKFNDEAKSMCQQLLAKSIKQRLGCRNGRMGGQDVMAHPFFHSTQLNWRRL 584

Human   457 EAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVSIPWQNEMIETECF 521
            |||||:|||||||.|||.||||||||||||||||:|.:|.:||:||:||||||.|||||:|||||
  Fly   585 EAGMLEPPFVPDPHAVYAKDVLDIEQFSTVKGVNIDESDTNFYTKFNTGSVSISWQNEMMETECF 649

Human   522 KELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSSPSSKTSFNHHINSNHVSSNS 586
            :|||||||.....|||..|..|||.|.|...  |:|:.:..:::.|....  .|.:.::|..|::
  Fly   650 RELNVFGPEECPTPDLQINAAPEPDKAGCFP--FRRKKKQPARTQPIPIP--EHLLTTSHSVSST 710

Human   587 TGSS 590
            |..|
  Fly   711 TVES 714

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
GRK5	NP_005299.1	N-terminal	1..185		96/307 (31%)
		Interaction with calmodulin	20..39		11/18 (61%)
		RGS	53..175	CDD:470619	53/245 (22%)
		STKc_GRK5	183..495	CDD:270780	240/316 (76%)
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:15542828	388..395		6/6 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	531..590		16/58 (28%)
		Sufficient for membrane localization	546..565		4/18 (22%)
Gprk2	NP_476867.1	RGS	35..>111	CDD:470619	32/75 (43%)
		RGS	<248..305	CDD:470619	28/57 (49%)
		STKc_GRK4_like	308..597	CDD:270756	218/288 (76%)
		S_TK_X	578..642	CDD:214529	48/63 (76%)