DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment KIF4B and kif4

DIOPT Version :9

Sequence 1:	NP_001092763.1	Gene:	KIF4B / 285643	HGNCID:	6322	Length:	1234	Species:	Homo sapiens
Sequence 2:	NP_957117.1	Gene:	kif4 / 393796	ZFINID:	ZDB-GENE-040426-1545	Length:	1248	Species:	Danio rerio

Alignment Length:	1272	Identity:	759/1272 - (59%)
Similarity:	957/1272 - (75%)	Gaps:	68/1272 - (5%)

- Green bases have known domain annotations that are detailed below.

Human     2 KEEVKGIPVRVALRCRPLVPKEISEGCQMCLSFVPGETQVVVGTDKSFTYDFVFDPCTEQEEVFN 66
            ||:.|.|||||||||||||||||:||||.||:|||.|.||:||.||:||:|:||||.||||||||
Zfish     3 KEDEKAIPVRVALRCRPLVPKEINEGCQCCLNFVPSEPQVIVGNDKAFTFDYVFDPTTEQEEVFN 67

Human    67 KAVAPLIKGIFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGIIPRVIQLLFKEIDKKSD 131
            .||:||:.|:||||:||||||||||||||:||||.||:|||||||||:|||||:.:|:|..|::|
Zfish    68 TAVSPLLSGLFKGYHATVLAYGQTGSGKTFSMGGTYTSEQENEPTVGVIPRVIRRIFQEKAKRTD 132

Human   132 FEFTLKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNN 196
            .||.|.||||||||||||||||.|::|..|:||||||:|||||||||:.||.|.:.|.|||.||:
Zfish   133 CEFVLAVSYLEIYNEEILDLLCTSKDKPVISIREDPKDGIKIVGLTERDVLNAHEMVCCLEMGNS 197

Human   197 SRTVASTAMNSQSSRSHAIFTISIEQRKKSDKNCSFRSKLHLVDLAGSERQKKTKAEGDRLKEGI 261
            :|||.|||||:.|||||||||||:|||:..||.....||||||||||||||||||||||||||||
Zfish   198 ARTVGSTAMNAASSRSHAIFTISLEQRRNGDKTDVMVSKLHLVDLAGSERQKKTKAEGDRLKEGI 262

Human   262 NINRGLLCLGNVISALGDD-KKGSFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLS 325
            :||||||.||||||||||: |||||||||||||||||||||||||||||||||||||||:|||::
Zfish   263 SINRGLLSLGNVISALGDESKKGSFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNIEETIN 327

Human   326 TLRYADRARKIKNKPIVNIDPHTAELNHLKQQVQQLQVLLLQAHGGTLPGSINAEPSENLQSLME 390
            |||||||||||||||::|:||..||:..||||||:|||:||.|.||..|....:||:||:..::|
Zfish   328 TLRYADRARKIKNKPVLNVDPRAAEMKRLKQQVQELQVMLLHARGGVAPVLSGSEPAENVSKVLE 392

Human   391 KNQSLVEENEKLSRCLSKAAGQTAQMLERIILTEQVNEKLNAKLEELRQHVACKLDLQKLVETLE 455
            :|:||.::|.||||.||::.||||.|.||:::.||.||||.:||:|||||.|||:|||:||||||
Zfish   393 QNRSLQDQNSKLSRELSESVGQTAAMFERMLMMEQTNEKLQSKLDELRQHAACKVDLQRLVETLE 457

Human   456 DQELKENVEIICNLQQLITQLSDETVACTAAAID-------TAVEEEAQVETS------------ 501
            ||||:||||:|.||||:|.:|..|: |..||.||       :::|..|....|
Zfish   458 DQELRENVEVIQNLQQIILELQRES-AGIAATIDAITSGNASSLEAAAHCSPSTAGAQAEICPGQ 521

Human   502 ----PETSRSSDAFTTQHALHQAQMSKEVVELNNALALKEALVRKMTQNDNQLQPIQFQYQDNIK 562
                ||...|.:|:|.||||.|||||||::|||..||||||.|:||.|||:.|:|:|.|||::|:
Zfish   522 DGAVPEAKDSPEAYTAQHALRQAQMSKELIELNKVLALKEAFVKKMCQNDSHLEPMQTQYQESIQ 586

Human   563 NLELEVINLQKEKEELVRELQTAKKNVNQAKLSEHRHKLLQELEGQIADLKKKLNEQSKLLKLKE 627
            :|:..|.:||||||:||:.||:|||:.|||||||.|.|.|||||||:.:|||||.:|||||||||
Zfish   587 SLQAAVGSLQKEKEDLVQTLQSAKKDTNQAKLSEQRRKRLQELEGQMTELKKKLQDQSKLLKLKE 651

Human   628 STERTVSKLNQEIWMMKNQRVQLMRQMKEDAEKFRQWKQKKDKEVIQLKERDRKRQYELLKLERN 692
            |:.|.|:||||||..||:||||||||||:|:||||.|||||||||:||||:|||||||:|||||:
Zfish   652 SSVRNVAKLNQEIQAMKSQRVQLMRQMKDDSEKFRLWKQKKDKEVLQLKEKDRKRQYEMLKLERD 716

Human   693 FQKQSSVLRRKTEEAAAANKRLKDALQKQREVTDKRKETQSHGKEGIAARVRNWLGNEIEVMVST 757
            ||||:|||||||||||||||||||||||:.||.:|||:.|:.|.|.:..||:|||.|||||:|||
Zfish   717 FQKQASVLRRKTEEAAAANKRLKDALQKRSEVAEKRKDVQNRGAESVTNRVKNWLLNEIEVLVST 781

Human   758 EEAKRHLNDLLEDRKILAQDVVQLKEKKESRENPPPKLRKCTFSLSEVHGQVLESEDCITKQIES 822
            |||:|||.|||||||:||:::.||:::.|:.|.|..|:|:.|.::||:.|: .|.|..|:||:||
Zfish   782 EEARRHLQDLLEDRKMLAEEISQLRQQMEAGEKPVSKVRRRTLTISELEGK-CELEASISKQVES 845

Human   823 LETEMELRSAQIADLQQKLLDAESEDRPKQCWENIATILEAKCALKYLIGELVSSKIHVTKLENS 887
            |:|||.||||||||||||:|||::|.|.||.|::|.||:|||.|||.|:.|:|:||....|.|:.
Zfish   846 LDTEMALRSAQIADLQQKVLDADNEGRMKQRWDSITTIIEAKSALKILMAEVVASKTLNAKQESE 910

Human   888 LRQSKASCADMQKMLFEEQNHFSEIETELQAELVRMEQQHQEKVLYLVSQLQESQMAEKQLEKSA 952
            |:|.||:..||||:|.:|:...|.::.|.|:.||.|||:||||||||:.|||...:|.::.::..
Zfish   911 LQQEKANLLDMQKVLCDERKLMSAMDMEHQSHLVEMEQRHQEKVLYLLGQLQNKPVALEEQQELV 975

Human   953 SEKEQQLVSTLQCQDEELEKMREVCEQNQQLLQENEIIKQKLILLQVASRQKHLP--NDTLLSPD 1015
            |::||:|:..||.|:||:||:||:.:|||:|.:|||..||||.|.|:.|.:|.|.  |:   |||
Zfish   976 SKREQELLQRLQFQEEEIEKLRELSDQNQKLKEENEQYKQKLHLAQLTSAKKVLSAVNE---SPD 1037

Human  1016 SSFEYIPPKPK-----PSRVKEKFLEQSMDIEDLKYCSEHSVNEHEDGDGDGDSDEGDDEEWKPT 1075
            :||||||||||     .:|.::   ::.:|:::|...|:               .|.:::||:|.
Zfish  1038 TSFEYIPPKPKAKMHSTARARQ---QEPIDLDELMSPSD---------------SEKEEDEWRPE 1084

Human  1076 KLVKVSR----KNIQGCSCKGWCGNKQCGCRKQKSDCGVDCSCDPTKCRNRQQGKDSLGTVEQTQ 1136
            |..|..|    ..:.||:|:|.|.||.|.|||.|..||.:|.||..|||| .:.:.|:...|.|.
Zfish  1085 KAEKTRRGSKKAKLNGCACRGRCVNKLCRCRKGKMTCGENCLCDHEKCRN-MENRSSVDLNETTD 1148

Human  1137 DSEGSFKL-EDPTEV-TPGLSFFNPVCATPNSKILKEMCDM--EQVLSKKTAPAPSPFDLPESKH 1197
            ..:.|..| ||||.| :...:||.|.|..|..|.:.|:..:  :..|.||..||.:.   .||..
Zfish  1149 VFKDSSVLPEDPTAVGSRDATFFTPPCVPPTKKEIGEVWQLPADLKLEKKPVPAENE---RESDS 1210

Human  1198 GATEYQQNKPPG-KKKKRALAS-NTSFFSGCSPIEEE 1232
            ..:|...|.... ::.||.|:: ..||||||:||.||
Zfish  1211 EDSEGMNNTSSFLRRNKRGLSNFKNSFFSGCTPIREE 1247

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
KIF4B	NP_001092763.1	KISc_KIF4	8..337	CDD:276823	261/329 (79%)
		KISc	9..343	CDD:214526	265/334 (79%)
		SbcC	406..995	CDD:223496	366/611 (60%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	494..513		6/34 (18%)
		Interaction with PRC1. /evidence=ECO:0000250\|UniProtKB:O95239	663..1234		290/587 (49%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	712..737		16/24 (67%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:O95239	793..798		2/4 (50%)
		Globular. /evidence=ECO:0000250	1000..1234		88/250 (35%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1007..1030		14/29 (48%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1052..1076		4/23 (17%)
		Pre-SET	<1072..1122	CDD:282838	25/53 (47%)
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000250\|UniProtKB:O95239	1086..1144		25/57 (44%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1122..1143		4/20 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1183..1234		19/52 (37%)
kif4	NP_957117.1	KISc_KIF4	9..339	CDD:276823	261/329 (79%)
		KISc	10..345	CDD:214526	265/334 (79%)
		BST2	568..644	CDD:293321	46/75 (61%)
		DUF3435	<938..1082	CDD:288746	68/164 (41%)
		TCR	1100..1136	CDD:281618	21/36 (58%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	911	1.000	Domainoid score	I564
eggNOG	1	0.900	-	-		E2759_KOG0244
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1444	1.000	Inparanoid score	I1046
NCBI	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D35421at7742
OrthoFinder	1	1.000	-	-		FOG0003140
OrthoInspector	1	1.000	-	-		otm27675
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	1	1.100	-	-	O	PTHR47969
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3568
SonicParanoid	1	1.000	-	-		X2108
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	13	12.860