Protein Alignment Pura and KALRN

DIOPT Version :10

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: XP_006713873.1
• Gene: KALRN / 8997
• HGNCID: 4814
• Length: 2988
• Species: Homo sapiens
• Alignment Length: 1825
• Identity: 393/1825 - (21%)
• Similarity: 656/1825 - (35%)
• Gaps: 571/1825 - (31%)

  Fly   448 SSRKEESVQSHCIDHENPGELPLSVLQLEMSTAAAALMASDCGELPQLL--QNLLVSVERS---- 506
            |...||...|...:||...||.||   ||....:|..:.|...:|.::|  :...|.||.|
Human   168 SQLTEEFDGSLDYNHEEWIELRLS---LEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLI 229

  Fly   507 IERVSLNE--LQMPMHHDRGEDSLDEANNNG--------CNNNLLGSPKCALRRSD---LITK-- 556
            .|...|.:  |:.|         ::|.:..|        |::...|. .|....:|   |:.|
Human   230 DEHTQLKKKVLKAP---------VEELDREGQRLLQCIRCSDGFSGR-NCIPGSADFQSLVPKIT 284

  Fly   557 ---NKLFNSR-----------------YMLRRLTNRQDSMNSTSSGNSNVSDRSSSSSSSSSSSA 601
               :||.::|                 :.||......:.|....|.|..:..:|.:....|...|
Human   285 SLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQYA 349

  Fly   602 GEELLTSHSEAKMRSNSVGNEASGASTSPALPLYCLGNSFPHIDSDEEASDSIGPGKHHS---LE 663
             .:|.|.|:...|.|.                     |::.:|:.....:..:....|::   ::
Human   350 -LDLQTQHNHFAMNSM---------------------NAYVNINRIMSVASRLSEAGHYASQQIK 392

  Fly   664 TCETELLP--SSSIAELPEK---LLTSGVY-LPGTRTLQGDPLVTVDAAC-VASAGINCYEMATL 721
            ...|:|..  .|..|.|.|:   |..|.|: ....:.|.|     |||.| :.|.|....||..|
Human   393 QISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSG-----VDAWCKMCSEGGLPSEMQDL 452

  Fly   722 LL---YYSTIPERSSCPFT--------------TPATPGT----TATINGHKSTEQ--------- 756
            .|   ::.|:.|:.:..:|              .|.:||.    |||.|..|:..|
Human   453 ELAIHHHQTLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVL 517

  Fly   757 --QQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQIGNCEILAVCPDSSL 819
              |::.:...|.:|           :.:.:...|||..   |.::.:...|.|       ...:.
Human   518 HHQRRLESIWQHRK-----------VRLHQRLQLCVFQ---QDVQQVLDWIEN-------HGEAF 561

  Fly   820 LQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVKFI-SVDQV--TTSVAPSQLPAG------ 875
            |....|..:...:.:..|..:.|..|.:..:..:..|.: :.:|:  |....|.::...
Human   562 LSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEV 626

  Fly   876 ----LLPSVPALSTLPGISQRHHDATKWREFFAQLEAFQRQCSTAGGRLVSALSEIRAADLQGLP 936
                .:..|.....|..:|...|..||  |.:..:|..|::          .|.::.|..:..:
Human   627 RIQDFVRRVEQRKLLLDMSVSFHTHTK--ELWTWMEDLQKE----------MLEDVCADSVDAV- 678

  Fly   937 TRRQLYGQHRALSRALMDSQLHSLRKMGAGQLARLQDLARAITSAPGSPRMTSQPQSSSSSSSGA 1001
              ::|..|.:....|.:|:.|:.:::.        :||.:.:.|||.|....|:.:.|:.|::
Human   679 --QELIKQFQQQQTATLDATLNVIKEG--------EDLIQQLRSAPPSLGEPSEARDSAVSNN-- 731

  Fly  1002 FLNVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALEDEISEVTSWMGSDGAEN 1066
              ....:..:..:..:..::|.|..::|:|..:|:.:|....:.|..|....|||:.:   .|.|
Human   732 --KTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAEL---DAWN 791

  Fly  1067 LQKFSQLHWDNESQLRTQEQEFEKY--YFIAMKHLA-----KGRDLHAAALKVSV---------- 1114
            .....|::..|...|...||..:::  ..:||.::.     :|:|||....:|..
Human   792 EDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYITEVQASGIELICEKD 856

  Fly  1115 --LSESANNLKSALDQFAQKLEL----AHERYDAAGRLLHLLTQHQR----------------EN 1157
              |:.....|...|.:...:|||    .|:|.:...:|.||..:.::                .|
Human   857 IDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVN 921

  Fly  1158 AA----REELQRLAEQ--------LGATSLLEKH---WPVMRKTHTLPVASSTPAAA---AGKRV 1204
            |:    .|:|||..||        ..||||.:.|   ..|.:|...|..|....|.|   ..::|
Human   922 ASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEVLLQAGHYDADAIRECAEKV 986

  Fly  1205 S-------------YRCSSASRSSFEGGPSSSNCSCWRESRNLEDMDQMEEEEEEQDHD-CDGGD 1255
            :             .:..:||.:.::  .|...||.      ||.:    |:|..:|.| |.|.|
Human   987 ALHWQQLMLKMEDRLKLVNASVAFYK--TSEQVCSV------LESL----EQEYRRDEDWCGGRD 1039

  Fly  1256 GEEQQSKI-------------ADSGLGVCDNCERN---------------PKLA----------- 1281
            .....::|             .::.|..|....||               |.:|
Human  1040 KLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVK 1104

  Fly  1282 -----------RICSCQSLNEKSHDELLEDECFDRPSKRYMDIMHSPMEANAHLQCHGS------ 1329
                       |:....:|.::..|:..:...|:|.:|:.:|.:....|  .:|..|.|
Human  1105 AILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGE--FYLSTHTSTGETTE 1167

  Fly  1330 ----------------------------------------------------------SLELPK- 1335
                                                                      ||.:.|
Human  1168 ETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKY 1232

  Fly  1336 ---LEELSCLDPKIQKTLLL----------------------------------IMREMIGTERD 1363
               ||:...::.:..|.|.|                                  ||.|::.||:.
Human  1233 RYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKA 1297

  Fly  1364 YVRSLYYVIENYIDELLR--EDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELERYERNPLKVGA 1426
            |||.|:..:|.|:.|:..  |:||..:..:.::|||||::|::|||:.||.|||:||:.|..||.
Human  1298 YVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGH 1362

  Fly  1427 AFLEMESKFYLYALYNKNKPKSDTLLSEYGSSFFKPKQMQLQDKLDLASYLLKPVQRMGKYALLL 1491
            .|:....||.:|..|.||||.|:.|:.|:..:||...|.:......::|||:|||||:.||.|||
Human  1363 CFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLL 1427

  Fly  1492 QQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNVKEQGRLLRQN 1556
            ::|:..|:..:|           ||:...|::....:..||.:.:..|...|.|:..||.|:.|:
Human  1428 KELLTCCEEGKG-----------ELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQD 1481

  Fly  1557 EFLVWQG----RGGKKTLRQVFLFEELVLFSKARRFPDHKNLDIYIYKNSIKTSDIGLTAHTGDS 1617
            .|.||..    |.|::  |.:||||..::|||  ...|......|:|||.:.||::|:|.|....
Human  1482 AFQVWDPKSLIRKGRE--RHLFLFEISLVFSK--EIKDSSGHTKYVYKNKLLTSELGVTEHVEGD 1542

  Fly  1618 ATKFEIWF-RKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREVRLAEMSSMGIGSKPCL 1681
            ..||.:|. |....|:..:|:..:.:.|..|.:.|           |||
Human  1543 PCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNI-----------REV---------------- 1580

  Fly  1682 DIRPSNNQISDRSIPL-------AQLNKTP-KLRHS----------EPGKGSMRRPNSLISESSL 1728
                    |.:|.|.|       .||.||| |.|::          ..|.|| .:|:::    |:
Human  1581 --------IQERIIHLKGALKEPLQLPKTPAKQRNNSKRDGVEDIDSQGDGS-SQPDTI----SI 1632

  Fly  1729 SSGTS--TTSGSSISGGS---------SSGHNDGGGRHSLHFGKL----------PGAHSASSTN 1772
            :|.||  |.....:|||.         |:||:   ...::..|:.          ||.....:|.
Human  1633 ASRTSQNTVDSDKLSGGCELTVVLQDFSAGHS---SELTIQVGQTVELLERPSERPGWCLVRTTE 1694

  Fly  1773 CSATLELINETQALKLAKSPSGGG-------IKGA---DTAADGGNTSGNGNGNG----NAFRSS 1823
            .|..||.:..:.||.::.|.|...       :|.|   .::.:||.:....|...    |:..||
Human  1695 RSPPLEGLVPSSALCISHSRSSVEMDCFFPLVKDAYSHSSSENGGKSESVANLQAQPSLNSIHSS 1759

  Fly  1824 KRNHKRSTTIV----------------------------SQLSMESGIVSDMCVTPDQEQSAAES 1860
            . ..||||..:                            .:.|.:.|.......|..|..||.|.
Human  1760 P-GPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEK 1823

  Fly  1861 SRSSW 1865
            |:..|
Human  1824 SKKGW 1828

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	42/189 (22%)
		RhoGEF	1353..1524	CDD:459876	68/172 (40%)
		PH_puratrophin-1	1524..1663	CDD:270062	41/143 (29%)
KALRN	XP_006713873.1	SEC14	41..179	CDD:214706	4/10 (40%)
		SPEC	192..412	CDD:238103	46/251 (18%)
		SPEC	313..538	CDD:238103	52/262 (20%)
		SPEC	539..768	CDD:238103	45/265 (17%)
		SPEC	893..1131	CDD:238103	47/249 (19%)
		SPEC	1137..1237	CDD:197544	11/101 (11%)
		RhoGEF	1286..1456	CDD:238091	68/180 (38%)
		PH1_Kalirin_Trio_like	1463..1585	CDD:270060	43/160 (27%)
		SH3_Kalirin_1	1651..1710	CDD:212786	12/61 (20%)
		SH3-RhoG_link	1708..1934	CDD:465196	24/122 (20%)
		RhoGEF	1935..2104	CDD:459876
		PH2_Kalirin_Trio_p63RhoGEF	2109..2243	CDD:270061
		SH3_Kalirin_2	2327..2385	CDD:212787
		I-set	2473..2567	CDD:400151
		Ig strand B	2490..2494	CDD:409570
		Ig strand C	2503..2507	CDD:409570
		Ig strand E	2533..2537	CDD:409570
		Ig strand F	2547..2552	CDD:409570
		Ig strand G	2560..2563	CDD:409570
		FN3	2571..2662	CDD:238020
		STKc_Kalirin_C	2692..2939	CDD:271017