Protein Alignment Pura and ROM2

DIOPT Version :10

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: NP_013475.1
• Gene: ROM2 / 851086
• SGDID: S000004363
• Length: 1356
• Species: Saccharomyces cerevisiae
• Alignment Length: 1322
• Identity: 253/1322 - (19%)
• Similarity: 450/1322 - (34%)
• Gaps: 412/1322 - (31%)

  Fly   782 IEKSQHLCVIDLICQSLR---LLAKQIGNCEILAVCPDSSLLQLCNGQQQQK-----LQQQ---- 834
            ||..|:|.|  |:.:.:.   ::|||.....:.|...||   .:.|.:.:.|     |.||
Yeast    49 IENIQNLNV--LLSEDIANDIIIAKQRRRSGVEAAIDDS---DIPNNEMKGKSSNYILSQQTNIK 108

  Fly   835 ----QQQNNNSDHPEPSSPS---------PVDLVKFISVDQVTTSVAPSQLPAGLLPSVPALSTL 886
                .|..:::|:...|||.         |:...|.:|....|::.|..|........:|.:|..
Yeast   109 EVPDTQSLSSADNTPVSSPKKARDATSSHPIVHAKSMSHIYSTSNSASRQAKHYNDHPLPPMSPR 173

  Fly   887 PGISQRHHDAT----KWREFFAQ--LEAFQRQCSTAGGRLVSALSEIRAADLQGL-----PTRRQ 940
            ..:.|::...|    |.:....|  |...::|.|.:.|...:..::.|.:.|||.     |:.:.
Yeast   174 NEVYQKNKSTTAFVPKRKPSLPQLALAGLKKQSSFSTGSASTTPTQARKSPLQGFGFFSRPSSKD 238

  Fly   941 LYGQHR---------------------------ALSRALMDSQLHSL--RKMGAGQLARLQDLAR 976
            |:.||:                           ..|.:......||:  |.|.... :.|:::|.
Yeast   239 LHEQHQHHQHIQHNNINNHNNNNTNNNGAHYQVGSSNSNYPQHSHSISSRSMSLNS-STLKNIAS 302

  Fly   977 AITSAPGSPR-------MTSQPQS-------------------------SSSSSSGAFLNVDAAC 1009
            :..|...:.|       :||.|.|                         :|||..|:..|:....
Yeast   303 SFQSKTSNSRKATQKYDITSNPFSDPHHHHHHHHSSNSHSSLNNVHGSGNSSSVMGSSSNIGLGL 367

  Fly  1010 KLRKVTLLFNEVDRAAQRL-----EQLTEQRRERLRQLTRQRALEDEISEVTSWMGSDGAENLQK 1069
            |.|     .:....|.:|.     ..|:..||..:..|:..|.:....|:......:..:....|
Yeast   368 KTR-----VSSTSLALKRYTSVSGTSLSSPRRSSMTPLSASRPVMSASSKKPQVYPALLSRVATK 427

  Fly  1070 FSQLHWDNESQLRTQEQEFEKYYFIAMKHLAKGRDL----HAAALKVSVLSESANNLKSALDQFA 1130
            |       :|.::..|.:.:...:         ||.    .|..:..:::..|..||  || .|.
Yeast   428 F-------KSSIQLGEHKKDGLVY---------RDAFTGQQAVDVICAIIRTSDRNL--AL-LFG 473

  Fly  1131 QKLELAHERYDAAGRLLHLLTQHQRENAAREELQRLAE--QLGATSLLEKHWPVMRKTHTLPVAS 1193
            :.|:        |.:|.|.:....|...:..|:....:  :...|.....|.|:|    .||.:|
Yeast   474 RSLD--------AQKLFHDVVYEHRLRDSPHEVYEFTDNSRFTGTGSTNAHDPLM----LLPNSS 526

  Fly  1194 STPAAAAGKRVSYRCSSASRSSFEGGPSSSNCSCWRESRNLEDMDQMEEEEEEQDHDCDGGDGEE 1258
            |..:.......|....|:|.||.....::...|....|.:|.     :::.....|:.:|
Yeast   527 SFNSGNHSYPNSGMVPSSSTSSLNSDQATLTGSRLHMSSSLS-----QQKNPAAIHNVNG----- 581

  Fly  1259 QQSKIADSGLGVCDNCERNPKLARICSCQS------------LNEKSHDEL-------LEDECFD 1304
                 ..:.|..|    .:|...|...|.|            ||.|.:..|       |:|:..:
Yeast   582 -----VFTLLAEC----YSPTCTRDALCYSISCPRRLEQQARLNLKPNGGLKRNISMALDDDDEE 637

  Fly  1305 RPSKRYMDIMHSPMEANAHLQCHGSSLELPKLEELSCLDPKIQKTLLLIMREMIGTERDYVRSLY 1369
            :||                   ..||:.....|.|...:.|.|:.:.    |:..||:::|:||
Yeast   638 KPS-------------------WTSSVSKEDWENLPKKEIKRQEAIY----EVYITEKNFVKSL- 678

  Fly  1370 YVIENYIDELLREDIPQPLRGQRNVIFGNIEKIFEFH-----------NSHFLGEL-ERYERNPL 1422
                    |:.|:...:.| .:.|:|..:|.|.|..|           |..||..| :|...:|:
Yeast   679 --------EITRDTFMKTL-AETNIISADIRKNFIKHVFAHINDIYSVNRRFLKALTDRQRSSPV 734

  Fly  1423 --KVGAAFLEMESKFYLYALYNKNKPKSDTLLSEYGS-----SFFKPKQMQLQDKLDLASYLLKP 1480
              .:|...|.....|..:..|..::|.:..|:....|     :.|....|....:..:.|:|.:.
Yeast   735 VRGIGDIVLRFIPFFEPFVSYVASRPYAKYLIETQRSVNPYFARFDDDMMSSSLRHGIDSFLSQG 799

  Fly  1481 VQRMGKYALLLQQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDC--- 1542
            |.|.|:|.||:::::|:...      ::..:|.|:|.:                |||:|||.
Yeast   800 VSRPGRYMLLVKEIMKSTDP------EKDKSDYEDLSK----------------AMDALRDFMKR 842

  Fly  1543 ------------DVNVKEQGRLLRQNEF-----------LVWQGRGGKKTLR----------QVF 1574
                        ||.:.:| ::|.:||:           :..:|...:|.|.          |.:
Yeast   843 IDQASGAAQDRHDVKLLKQ-KILFKNEYVNLGLNDERRKIKHEGILSRKELSKSDGTVVGDIQFY 906

  Fly  1575 LFEELVLFSKAR---RFPDHKNLDIYIYKNSIKT--------SDI-GLTAHTGD----SATKFEI 1623
            |.:.::||.||:   ::..||     :::..|..        .|: .|..:.||    |.|..:.
Yeast   907 LLDNMLLFLKAKAVNKWHQHK-----VFQRPIPLPLLFACPGEDMPALRKYIGDHPDCSGTVIQP 966

  Fly  1624 WFRKRKPDD--TWM-------------------LQCMSEDIKNAWTEEISKL----LWKQAKR-- 1661
            .:....|.:  |::                   ||.:.|.||......|||.    :.|.:.|
Yeast   967 EYNTSNPKNAITFLYYGAKQRYQVTLYAAQYAGLQTLLEKIKQGQAAIISKTEMFNVTKMSDRFF 1031

  Fly  1662 --------------NREVRLAEMSSM---GIGSKPCLDIRPSNNQISDRSIPLAQLNKTPKLRHS 1709
                          .|::.:|..|.:   .|..:...|.|..::......|.|.|.|...::...
Yeast  1032 DYTNKINSVTSCDGGRKLLIATNSGLYMSNIKRQQNKDHRHKSSAFFSTPIQLVQRNNITQIAVL 1096

  Fly  1710 EPGKGSMRRPNSLISESSLSSGTSTTSGSS---------ISGGSSSGHNDGGGRHSLHFGKLPGA 1765
            |..|..:...:..:....||...:..:|:|         |:..|.....|..|:.     .:..|
Yeast  1097 EEFKSIILLIDKKLYSCPLSLIEAEGNGTSFFKKHHKELINHVSFFAEGDCNGKR-----LIVTA 1156

  Fly  1766 HSASSTNCSATLELINETQALKLAKSPSGGGIKGADTAADGGNTSGNGNGNGNAFRSSKRNHKRS 1830
            ||:|.     :::.......|...|                     ||:|:||     |::.|:.
Yeast  1157 HSSSH-----SIKYFEHEHPLLAEK---------------------NGSGSGN-----KKSLKKK 1190

  Fly  1831 TTIV---SQLSMESGIVSDMCV 1849
            .|.|   |:....|.:.:::|:
Yeast  1191 ITEVIFDSEPVSISFLKANLCI 1212

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	27/155 (17%)
		RhoGEF	1353..1524	CDD:459876	42/189 (22%)
		PH_puratrophin-1	1524..1663	CDD:270062	42/231 (18%)
ROM2	NP_013475.1	ROM1	4..1356	CDD:227709	253/1322 (19%)