DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pura and kalrnb

DIOPT Version :9

Sequence 1:	NP_996016.2	Gene:	Pura / 2768948	FlyBaseID:	FBgn0035802	Length:	1904	Species:	Drosophila melanogaster
Sequence 2:	NP_001314918.1	Gene:	kalrnb / 678652	ZFINID:	ZDB-GENE-060421-7244	Length:	2306	Species:	Danio rerio

Alignment Length:	1798	Identity:	387/1798 - (21%)
Similarity:	649/1798 - (36%)	Gaps:	485/1798 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly   461 DHENPGELPLSVLQLEMSTAAAALMASDCGELPQLL--QNLLVSVE-------------RSIERV 510
            ||....||.:|   ||..|..|..:.|...||.::|  |.|..|.|             :::.:.
Zfish   186 DHVEWTELRVS---LEEFTGGALHLLSRLEELQEVLSRQELASSAEEARKLLEDHARLRKTMAKA 247

  Fly   511 SLNELQMPMHHDRGEDSLDEANNNGCNNNLLGS-------PKCALRRSDL-ITKNKL---FNSR- 563
            .::||.     ..|:..|.:..:.| :..|.|.       ||.:.....| :|:..|   :::|
Zfish   248 PVDELD-----QEGQRLLQKIRDGG-DGRLSGGADFQSLVPKISALLDKLQVTRQHLLQGWHNRK 306

  Fly   564 ------YMLRRLTNRQDSMNSTSSGNSNVSDRSSSSSSSSSSSAGEELLTSHSEAKMRS-NSVGN 621
                  :.||......:.|......|..:..::.:....|...|. :|.|.|....|.| |:..|
Zfish   307 QQLDQCFQLRLYEQDAEKMFEWIGHNKELFLQTHTDIGVSYQHAA-DLQTQHDHFAMNSMNAYVN 370

  Fly   622 EASGASTSPALPLYCLGNSFP---------HIDSDEEASDSIGPGKHHSLETCETELLPSSSIAE 677
            .:...|.:..|   |....:.         .:|.|.::..|       .||.....|..||...:
Zfish   371 ISRIVSVATRL---CEAGHYAATQIQQISGQLDHDWKSFAS-------GLEERSAILAMSSVFHQ 425

  Fly   678 LPEKLLTSGVYLPGTRTLQGD---PLVTVDAACVASAGINCYEMATLLLYYSTIPERSSCPFTT- 738
            ..|:.|:|   :.|.....|:   |.||.:.........|.||..|..  |:.:.::.......
Zfish   426 KSEQFLSS---MEGWVKSCGEVGLPTVTQELELAIHNHQNLYEQVTTA--YTEVSQKGKTLLDVL 485

  Fly   739 -----PATPGT-------TATING---------------------HKSTEQQQQQ----QQQQQQ 766
                 ||..|:       :..:.|                     ||....|:.|    ||..||
Zfish   486 QRPPPPADSGSLTAGADYSQAVRGVLEVVHEVVHQYRRLEGLLQHHKLRLHQRLQLCVFQQDVQQ 550

  Fly   767 -----------------------------QK-----QQPAQQTFTILIAIEKSQHLCVIDLICQS 797
                                         ||     |:.||.|:|            ..|.:.::
Zfish   551 VLDWIENHGEAFLSKHTGVGKSVHRARALQKRHDDFQEVAQNTYT------------NADKLLEA 603

  Fly   798 LRLLAKQIGNC---EILAVCP----------------------------------------DSSL 819
            ...|| |.|.|   ||.|...                                        ..||
Zfish   604 AEQLA-QTGECDPEEIYAAAHHLEVRVQEFVRRVEQRKLLLDISVSFHTHTKELWSWMEDLQKSL 667

  Fly   820 LQ-LCNGQQQQKLQQ-----QQQQNNNSDHPEPSSPSPVDLVKFISVDQVTTSVAPSQLPAGLLP 878
            :: :.|.:....:|:     ||||::..|       :.::::|  ..:::...:..|.|....||
Zfish   668 VEPVVNSESVDAVQELIRQFQQQQSSTLD-------ATLNVIK--EGEELIQHLRDSALACNKLP 723

  Fly   879 SVPALSTLPGISQRHHDATKWRE-----------FFAQLEAFQRQCSTAGGRLVSALSEI--RAA 930
            ...:::.:.|:.|:..:|....|           .|.||..|::......|.|.:...::  :|.
Zfish   724 HASSVAHIEGVLQQLDEAQAHMEELFHERRIKLDIFLQLRIFEQYALEVMGELDAWKQDLMRQAT 788

  Fly   931 DL--QGLPTRRQLYGQHRALSRAL-MDSQLHSLRKMGAGQLARLQDLARAITSAPGSPRMTSQPQ 992
            |.  :.|....|..  ||...|.| |::..:.:.:.|       |||.:.|          .:.|
Zfish   789 DFNTEELTLAEQRL--HRHTERKLAMNNMTYEVIQQG-------QDLHQYI----------MEVQ 834

  Fly   993 SSSSSSSGAFLNVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALEDEISEVTS 1057
            :|....:|. .:||.|.|::::....||   ....||...|...:||.|..:.|.|:.|:.:|..
Zfish   835 ASGMELTGE-KDVDLAAKVQELLEFLNE---KQHELEVSAEHTHKRLEQCLQLRHLQAEVKQVLG 895

  Fly  1058 WMGSDGAENLQKFSQLHWDNESQLRTQEQEFEKYYFIAMKHLAKGRDL---HAAALKVSVLSE-- 1117
            |:.:  .|::...|..:....|::...::|.|::. :|::.|.....|   |.:||::...:|
Zfish   896 WIRN--GESMLTASTTNAGPLSEVEQLQREHEQFQ-LAIESLLHADSLQRTHQSALQLQQRAELL 957

  Fly  1118 -----------------SANNLKSALDQFAQKLELAHER---YDAAGRLLHLLTQHQRENAAREE 1162
                             .|.:.::.:.:...:|:|.:..   |..:.::.::|...:.|....|:
Zfish   958 LQAGHYDPDAVRACAETVALHWQTLMLKMEDRLKLVNASVAFYKTSEQVCNVLESLELEYRREED 1022

  Fly  1163 LQRLAEQLGATSLLEKHWPVMRKTHTLPVASSTPAAAAGKR---VSYRCSSASRSSFEGGPSSSN 1224
            .....::||:|:..:...|::.| |.....:...|....:|   |..:....:..|..|..:.:.
Zfish  1023 WCGGHDKLGSTAETDHVSPLINK-HLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPGVVTHNR 1086

  Fly  1225 CS-----------CWRESRNL------------------------EDMDQMEEEEEE--QDHDCD 1252
            .:           ..||:|.|                        :.:|.::|..|.  ..|...
Zfish  1087 ATESQVKAILSELLQRENRVLHFWTMKKRRLDQCQQYVLFERHAKQAIDWLQETGEYFLSTHTSS 1151

  Fly  1253 GGDGEEQQ---SKIADSGLGVCDNCERNPKLARICSCQSLNEKSHDELLE-DECFDRPSKRYMDI 1313
            |...|:.|   .:..|..:......|:...|.::..  ||.||.|..:.| ........:||.|.
Zfish  1152 GETSEKAQELLKEYEDFRVSAKQTQEKVKLLIQLAG--SLVEKGHVHVTELKRWVSTVDRRYRDF 1214

  Fly  1314 MHSPMEANAHLQC-----------HGSSLELPKL--------EELSCLDP---------KIQKTL 1350
                .....|.:|           ....|||..:        .|::..||         |..:..
Zfish  1215 ----SMRMGHYRCSLDRALGVCSEDNKDLELDIIPANLTHTDPEVNLNDPDHEVNEEKKKSARKK 1275

  Fly  1351 LLIMREMIGTERDYVRSLYYVIENYIDELLR-EDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGEL 1414
            ..||.|::.|||.|||.|...:|.:..|:.. .::|..:..:.:||||||::|::|||:.||.||
Zfish  1276 EYIMAELLQTERAYVRDLQECLETFFWEMTSGVEVPSGIANKEHVIFGNIQEIYDFHNNIFLKEL 1340

  Fly  1415 ERYERNPLKVGAAFLEMESKFYLYALYNKNKPKSDTLLSEYGSSFFKPKQMQLQDKLDLASYLLK 1479
            |.||:.|..||..|:....||::|..|.||||.|..|:......||...|.:......::|||:|
Zfish  1341 ENYEQLPEDVGHCFVTWADKFHMYVTYCKNKPDSSQLIQGQAGRFFDEIQRRHGLANSISSYLIK 1405

  Fly  1480 PVQRMGKYALLLQQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDV 1544
            ||||:.||.|||::|:..|:..:|           |::...|::....:..||.:.:..|...|.
Zfish  1406 PVQRITKYQLLLKELLTCCEEGKG-----------EIKEGLEVMLSVPKRANDAMHVSMLEGFDE 1459

  Fly  1545 NVKEQGRLLRQNEFLVWQG----RGGKKTLRQVFLFEELVLFSKARRFPDHKNLDIYIYKNSIKT 1605
            |:..||.|:.|:.|.||..    |.|:.  |.:||||..::|||..:  |......|:|||.:.|
Zfish  1460 NLAVQGELILQDTFQVWDPKSLIRKGRD--RHLFLFEISLVFSKEMK--DSSGRTKYVYKNRLLT 1520

  Fly  1606 SDIGLTAHTGDSATKFEIWF-RKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREVRLAE 1669
            |::|:|.|......||.:|. |....|:..:|:..|.::|..|...|.:::     :.|.|.|
Zfish  1521 SELGVTEHIEGDPCKFALWAGRTPTSDNKTVLKASSIEVKQEWIRNIREVI-----QERTVHL-- 1578

  Fly  1670 MSSMGIGSKPCLDIRPSNNQISDRSIPLAQLNKTPKLR---HSEPGKGSM--RRPNSLISESSLS 1729
               .|...:|   |.........|||...:..:....:   .|:|...|:  |...:.:....||
Zfish  1579 ---KGALKEP---IHAPKTPARQRSISKRETTEDADSQGDASSQPDTISIASRTSQNTVDSDKLS 1637

  Fly  1730 SGTSTT-------SGS----SISGGSS------SGHNDG------GGRHSLHFGKLPG-----AH 1766
            .|...|       :||    ||..|.:      ||...|      ........|.:|.     :|
Zfish  1638 GGCEQTVVLQDFVAGSVGELSIQSGQTVELLERSGERPGWCLVRTTENSPPQEGLVPSSTLSVSH 1702

  Fly  1767 SASSTNCS------------ATLELINETQA-----LKLAKSPSGG-GIKGADTAADGGNT---- 1809
            |.||.:..            .:.|:.:|:.|     ..:...|:|. |.|.:.|.   |||
Zfish  1703 SRSSVDMDCFFPPGKENYPVGSSEVKSESAASLQPQTSVTSLPTGSPGPKRSGTT---GNTLKKW 1764

  Fly  1810 ---------SGNGNGNGNAFRSSKRNHKRSTTIVSQLSMESGIVSDMCVTPDQEQSAAESSRSSW 1865
                     .|.|: |.||.:.:.:..||.    .:.|:|.|        |..:.|..|..|.
Zfish  1765 LTSPVRRLSHGKGD-NANAKKPNNKQRKRD----GRKSVELG--------PHHQDSTEERGRR-- 1814

  Fly  1866 STSTTGASGASTTSASTV-----ILRRYRSYAHAAESASPESG 1903
            .|::.|...|.....:.:     |::.    ..|.|..:||.|
Zfish  1815 GTNSGGEEEAEDEPHTPLPPPMEIIKD----PSAQEDKTPERG 1853

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	PX_domain	15..>52	CDD:295365
		RhoGEF	1353..1532	CDD:279015	65/179 (36%)
		PH_puratrophin-1	1524..1663	CDD:270062	42/143 (29%)
		PH	1562..1655	CDD:278594	30/97 (31%)
kalrnb	NP_001314918.1	SEC14	46..184	CDD:214706
		SPEC	197..413	CDD:238103	46/232 (20%)
		SPEC	540..757	CDD:238103	39/238 (16%)
		SPEC	882..1120	CDD:238103	39/241 (16%)
		SPEC	1126..1214	CDD:197544	18/89 (20%)
		RhoGEF	1277..1446	CDD:238091	65/179 (36%)
		PH1_Kalirin_Trio_like	1453..1575	CDD:270060	40/130 (31%)
		PH	1486..1571	CDD:278594	28/88 (32%)
		SH3_Kalirin_1	1641..1700	CDD:212786	11/58 (19%)
		SH3-RhoG_link	1697..>1864	CDD:293215	38/179 (21%)
		RhoGEF	1886..2056	CDD:279015
		PH2_Kalirin_Trio_p63RhoGEF	2060..2193	CDD:270061
		PH	2083..2178	CDD:278594
		SH3	2246..2302	CDD:302595
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0689
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810