Protein Alignment Pura and mcf2lb

DIOPT Version :10

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: XP_068079741.1
• Gene: mcf2lb / 569231
• ZFINID: ZDB-GENE-081104-416
• Length: 1104
• Species: Danio rerio
• Alignment Length: 1298
• Identity: 291/1298 - (22%)
• Similarity: 481/1298 - (37%)
• Gaps: 342/1298 - (26%)

  Fly   659 HHSLETC---ETELLPSSS----IAELPEKLLTSGVYLPGTRTLQGDPLVTVDAACVASAGINCY 716
            :|....|   :.|::...|    .|::..:|.....:|.|.|...|.|:: :.....:...|...
Zfish    21 YHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPII-IFPEYPSFCEIGDQ 84

  Fly   717 EMATLLLYYSTIPERSSCPFTTPATPGTTATINGHKSTEQQQQQQQQQQQQKQQPAQQTFTILIA 781
            |...:|.|.::||..|:                                      |...|.::|.
Zfish    85 EFRNVLTYLTSIPSLSA--------------------------------------AGVGFIVVID 111

  Fly   782 IEKSQHLCVIDLICQSLRLLAKQIGNCEILAVCPDSSLLQLCNGQQQQKLQQQQQQNNNSDHPEP 846
            ..:.:..|:...:   ||:......|..::.|...|::||........||.:     ::...|..
Zfish   112 RRQDRWTCLKGTL---LRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHR-----DDFKVPVI 168

  Fly   847 SSPSPVDLVKFISVDQVTTSVAPSQLPAGLLPSVPALSTLPGISQRHHDATKWREFFAQLEAFQR 911
            ...|..||..:|..:|:|..:..||                     ::....|......||:|
Zfish   169 MLSSVADLHTYIERNQLTQELGGSQ---------------------YYCHKTWISHRTDLESF-- 210

  Fly   912 QCSTAGGRLVSALSEIRAADLQGLPTRRQLYGQHRALSRALMDSQLHSLRKMGAGQLARLQDLAR 976
                             ||.::.:..|.|::|      |.|.:::|
Zfish   211 -----------------AALVKRMAQRLQVFG------RELAETEL------------------- 233

  Fly   977 AITSAPGSPRMTSQPQSSSSSSSGAFLNVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQ 1041
                          |.:..::||  .||...:.|    .:...::..|..:..::.|..||.:|:
Zfish   234 --------------PSNHLTASS--LLNTHTSRK----DIFKEDMAGALSQGRKILENIREPVRR 278

  Fly  1042 LTRQRALEDEISEVTSWMGSDGAENLQKFSQLHWDNESQLRTQEQEFEKYYFIAMKHLAKGRDLH 1106
                        :..|.:..|..|||....:|    .|||......|::::   ::|..|    .
Zfish   279 ------------DPDSSLNPDQLENLATVHRL----LSQLNESSTAFDEFW---IQHQNK----L 320

  Fly  1107 AAALKVSVLSESANNLKSALDQFAQKLELAHERYDAAG-------RLLHLLTQHQRENAAREELQ 1164
            ...|||.....:...|::.||:..:.|    ..:.|.|       .||..||.|:::  ..|.|.
Zfish   321 ELCLKVCQFEHNFQQLRTELDRATETL----NAFSAVGISPAQTEHLLQELTNHEKK--VCEVLD 379

  Fly  1165 R----LAEQLG---ATSLLEKHWPVMRKTHTLPVASS-------------TPAAAAGKRVSYRCS 1209
            |    :||..|   ::.:|:.  .:..|...|..||.             |.|....||:...|.
Zfish   380 RVRSLVAEGQGLIDSSQILDD--SIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCK 442

  Fly  1210 --------SASR-----SSFEGGPSS-SNCSCWRESRNLEDMDQMEEEEEEQDHDCDGGDGEEQQ 1260
                    .||:     .|.||..|: |...|:.|:.|.:...::....:|.|:..: .:..||.
Zfish   443 WCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILN-NELREQV 506

  Fly  1261 SKIADSGLGVCDNCERN----PKLAR---------ICSCQSLNEKSHDELLEDECFDRPSKRYMD 1312
            ::..:....:.:..:|.    .|||.         ..|.:||:..:|.: .|..|....|    |
Zfish   507 TRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRD-HESICISEDS----D 566

  Fly  1313 IMHSPMEANAHLQCHGSSLELPKLEELSCLDPKIQKTLLL----IMREMIGTERDYVRSLYYVIE 1373
            ...|..:.|:. |...||......||        ::||.:    :|.|::.|||.||..|..|::
Zfish   567 SRGSCKQINSD-QVDRSSRNASVSEE--------EETLAVLRRHVMNELLETERAYVEELMCVLQ 622

  Fly  1374 NYIDEL----LREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELERYERNPLKVGAAFLEMESK 1434
            .|..|:    :...||..|:.:::|:|||:::|:.||...||.|||.|...|..||..||:...:
Zfish   623 GYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGE 687

  Fly  1435 FYLYALYNKNKPKSDTLLSEYGS-SFFKPKQMQLQDKLDLASYLLKPVQRMGKYALLLQQLVKAC 1498
            ..:|..|..|||:|::|..:... :||:..|.:|:.||.|.|||||||||:.||.||:::::|..
Zfish   688 LQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYS 752

  Fly  1499 KGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNVKEQGRLLRQNEFLVW-- 1561
            ||.||:.         |||.|...:...|:..||.:.:.::...|.|:.:.||||.|..|.||
Zfish   753 KGCEGSV---------ELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAE 808

  Fly  1562 QGRGGKKTL---------RQVFLFEELVLFSKARR--FPDHKNLDIYIYKNSIKTSDIGLTAHTG 1615
            ..||..|.:         |.:||.|:.:||.|.|.  ...::....|.:|..:..:.||:|.|..
Zfish   809 HKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAK 873

  Fly  1616 DSATKFEIWFRKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREVRLAEMSSMGIGSKPC 1680
            ..:.|||||...|  |:.:.:|..||::|..|..||.|||..|.:..:|
Zfish   874 GDSKKFEIWSSSR--DEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKE---------------- 920

  Fly  1681 LDIRPSNNQISDRSIPLAQLNKTPKLRHSEPGKGSMRRPNSLISESSLSSGTSTTSGSSI--SGG 1743
                              ::.:.||...|:     .|.|....||||.::.......||:  .|.
Zfish   921 ------------------RIFEEPKNEASQ-----QRAPEQFTSESSSTNRLVRNERSSLKSDGV 962

  Fly  1744 SSSGHNDGGGRHSLHFGKLPGAHSASSTNCSATLELINETQALKLAK--------SPSGGGIKGA 1800
            ......:..|:.                     ||.|.|::..:.||        .|....::..
Zfish   963 EKQKREEERGKE---------------------LESIFESRTAERAKGSSFSFETKPKRQDVRSD 1006

  Fly  1801 DTAADGGNTSGNGNGNGNAFRSSKRNHKRSTTIVSQLSMESGIVSDMCVTPDQEQSAAESSRS 1863
            .|..:.|.....|.....:..|..::.:|.|.......|:..:..|..|..|...||.|.:.|
Zfish  1007 PTPLETGPHPNLGGVRWFSTSSLFQSRRRGTHTEYTCWMKGSLSLDASVEHDGYFSAEEQANS 1069

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	34/157 (22%)
		RhoGEF	1353..1524	CDD:459876	68/175 (39%)
		PH_puratrophin-1	1524..1663	CDD:270062	48/151 (32%)
mcf2lb	XP_068079741.1	CRAL_TRIO_2	66..197	CDD:463965	31/198 (16%)
		SPEC	328..>473	CDD:413338	34/152 (22%)
		RhoGEF	599..776	CDD:238091	69/185 (37%)
		PH-like	783..914	CDD:473070	44/132 (33%)