Protein Alignment Pura and Kalrn

DIOPT Version :10

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: XP_011244253.1
• Gene: Kalrn / 545156
• MGIID: 2685385
• Length: 2999
• Species: Mus musculus
• Alignment Length: 1821
• Identity: 391/1821 - (21%)
• Similarity: 663/1821 - (36%)
• Gaps: 544/1821 - (29%)

  Fly   448 SSRKEESVQSHCIDHENPGELPLSVLQLEMSTAAAALMASDCGELPQLL--QNLLVSVERS---- 506
            |...||...|...:||...||.||   ||....:|..:.|...:|.::|  :...|.||.|
Mouse   174 SQLTEEFDGSLDYNHEEWIELRLS---LEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLI 235

  Fly   507 IERVSLNE--LQMPMHHDRGEDSLDEANNNG--------CNNNLLGSPKCALRRSD---LITK-- 556
            .|...|.:  |:.|         ::|.:..|        |::...|. .|....:|   |:.|
Mouse   236 DEHTQLKKKVLKAP---------VEELDREGQRLLQCIRCSDGFSGR-NCIPGSADFQSLVPKIT 290

  Fly   557 ---NKLFNSR-----------------YMLRRLTNRQDSMNSTSSGNSNVSDRSSSSSSSSSSSA 601
               :||.::|                 :.||......:.|....|.|..:..:|.:....|...|
Mouse   291 SLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHA 355

  Fly   602 GEELLTSHSEAKMRSNSVGNEASGASTSPALPLYCLGNSFPHIDSDEEASDSIGPGKHHS---LE 663
             .:|.|.|:...|.|.                     |::.:|:.....:..:....|::   ::
Mouse   356 -LDLQTQHNHFAMNSM---------------------NAYVNINRIMSVASRLSEAGHYASQQIK 398

  Fly   664 TCETELLP--SSSIAELPEK---LLTSGVY-LPGTRTLQGDPLVTVDAAC-VASAGINCYEMATL 721
            ...|:|..  .|..|.|.|:   |..|.|: ....:.|.|     |||.| :.|.|....||..|
Mouse   399 QISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSG-----VDAWCKMCSEGGLPSEMQDL 458

  Fly   722 LL---YYSTIPERSSCPFT--------------TPATPGT----TATINGHKSTEQ--------- 756
            .|   ::.::.|:.:..:|              .|.:||.    |||.|..|:..|
Mouse   459 ELAIHHHQSLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVL 523

  Fly   757 --QQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQIGNCEILAVCPDSSL 819
              |::.:...|.:|           :.:.:...|||..   |.::.:...|.|       ...:.
Mouse   524 HHQRRLESIWQHRK-----------VRLHQRLQLCVFQ---QDVQQVLDWIEN-------HGEAF 567

  Fly   820 LQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVKFI-SVDQV--TTSVAPSQLPAG------ 875
            |....|..:...:.:..|..:.|..|.:..:..:..|.: :.:|:  |....|.::...
Mouse   568 LSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEV 632

  Fly   876 ----LLPSVPALSTLPGISQRHHDATKWREFFAQLEAFQRQCSTAGGRLVSALSEIRAADLQGLP 936
                .:..|.....|..:|...|..||  |.:..:|..|::          .|.::.|..:..:
Mouse   633 RIQDFVRRVEQRKLLLDMSVSFHTHTK--ELWTWMEDLQKE----------VLEDVCADSVDAV- 684

  Fly   937 TRRQLYGQHRALSRALMDSQLHSLRKMGAGQLARLQDLARAITSAPGSPRMTSQPQSSSSSSSGA 1001
              ::|..|.:....|.:|:.|:.:::.        :||.:.:.|||.|....::.:.|:.|::
Mouse   685 --QELIKQFQQQQTATLDATLNVIKEG--------EDLIQQLRSAPPSLGEPTEARDSAMSNN-- 737

  Fly  1002 FLNVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALEDEISEVTSWMGSDGAEN 1066
              ....:..:..:..:..::|.|..::|:|..:|:.:|....:.|..|....|||:.:   .|.|
Mouse   738 --KTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIEVTAEL---DAWN 797

  Fly  1067 LQKFSQLHWDNESQLRTQEQEFEKY--YFIAMKHLA-----KGRDLHAAALKVSV---------- 1114
            .....|::..|...|...||..:::  ..:||.::.     :|:|||...::|..
Mouse   798 EDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKD 862

  Fly  1115 --LSESANNLKSALDQFAQKLEL----AHERYDAAGRLLHLLTQHQR----------------EN 1157
              |:.....|...|.:...:|||    .|:|.:...:|.||..:.::                .|
Mouse   863 LDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVN 927

  Fly  1158 AA----REELQRLAEQ--------LGATSLLEKH---WPVMRKTHTLPVASSTPAAA---AGKRV 1204
            |:    .|:|||..||        ..||||.:.|   ..|.:|...|..|....|.|   ..::|
Mouse   928 ASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKV 992

  Fly  1205 S-------------YRCSSASRSSFEGGPSSSNCSCWRESRNLEDMDQMEEEEEEQDHD-CDG-- 1253
            :             .:..:||.:.::  .|...||.      ||.:    |:|..:|.| |.|
Mouse   993 ALHWQQLMLKMEDRLKLVNASVAFYK--TSEQVCSV------LESL----EQEYRRDEDWCGGRD 1045

  Fly  1254 --GDGEEQQSKI---------ADSGLGVCDNCERN---------------PKLA----------- 1281
              |...|....|         .::.|..|....||               |.:|
Mouse  1046 KLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSMPSVASHTRGPEQQVK 1110

  Fly  1282 -----------RICSCQSLNEKSHDELLEDECFDRPSKRYMDIMHSPMEANAHLQCHGS------ 1329
                       |:....:|.::..|:..:...|:|.:|:.:|.:....|  .:|..|.|
Mouse  1111 AILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAKQALDWIQETGE--YYLSTHTSTGETTE 1173

  Fly  1330 ----------------------------------------------------------SLELPK- 1335
                                                                      ||.:.|
Mouse  1174 ETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGHIHATEIRKWVTTVDKHYRDFSLRMGKY 1238

  Fly  1336 ---LEELSCLDPKIQKTLLL----------------------------------IMREMIGTERD 1363
               ||:...::.:..|.|.|                                  ||.|::.||:.
Mouse  1239 RYSLEKALGVNTEDNKDLELDIIPASLSDREVKLRDANHEINEEKRKSARKKEFIMAELLQTEKA 1303

  Fly  1364 YVRSLYYVIENYIDELLR--EDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELERYERNPLKVGA 1426
            |||.|:..:|.|:.|:..  |:||..:..:.::|||||::|::|||:.||.|||:||:.|..||.
Mouse  1304 YVRDLHECLETYLWEMTSGVEEIPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGH 1368

  Fly  1427 AFLEMESKFYLYALYNKNKPKSDTLLSEYGSSFFKPKQMQLQDKLDLASYLLKPVQRMGKYALLL 1491
            .|:....||.:|..|.||||.|:.|:.|:..:||...|.:......::|||:|||||:.||.|||
Mouse  1369 CFVTWADKFQMYVTYCKNKPDSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLL 1433

  Fly  1492 QQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNVKEQGRLLRQN 1556
            ::|:..|:..:|           ||:...|::....:..||.:.:..|...|.|:..||.|:.|:
Mouse  1434 KELLTCCEEGKG-----------ELKDGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQD 1487

  Fly  1557 EFLVWQG----RGGKKTLRQVFLFEELVLFSKARRFPDHKNLDIYIYKNSIKTSDIGLTAHTGDS 1617
            .|.||..    |.|::  |.:||||..::|||  ...|......|:|||.:.||::|:|.|....
Mouse  1488 AFQVWDPKSLIRKGRE--RHLFLFEISLVFSK--EIKDSSGHTKYVYKNKLLTSELGVTEHVEGD 1548

  Fly  1618 ATKFEIWF-RKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREVRLAEMSSMGIGSKPCL 1681
            ..||.:|. |....|:..:|:..:.:.|..|.:.|           |||
Mouse  1549 PCKFALWSGRTPSSDNKTVLKASNIETKQEWIKNI-----------REV---------------- 1586

  Fly  1682 DIRPSNNQISDRSIPL-------AQLNKTP-KLRHS----------EPGKGSMRRPNSLISESSL 1728
                    |.:|.|.|       .||.||| |||::          ..|.|| .:|:::    |:
Mouse  1587 --------IQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGS-SQPDTI----SI 1638

  Fly  1729 SSGTS--TTSGSSISGGS---------SSGHNDGGGRHSLHFGKL----------PGAHSASSTN 1772
            :|.||  |.....:|||.         |:||:   ...|:..|:.          ||.....:|.
Mouse  1639 ASRTSQNTVESDKLSGGCELTVVLQDFSAGHS---SELSIQVGQTVELLERPSERPGWCLVRTTE 1700

  Fly  1773 CSATLELINETQALKLAKSPSGGGIKGADTAADGGNTSGNGNGNGNAFRSSKRNHKRSTTIVSQL 1837
            .|...|.:..:.||.::.|.|...:.......|..:.|.:.||.    :|....|     :.||.
Mouse  1701 RSPPQEGLVPSSALCISHSRSSVEMDCFFPLKDSYSHSSSENGG----KSESVAH-----LQSQP 1756

  Fly  1838 SMESGIVSDMCVTPDQEQSAAESSRSSWSTSTTGASGASTTSASTVILRRYRSYAHAAESASPES 1902
            |:.|     :..:|..::|.  ::...|.||......:.....:....::.|....:.:..||:.
Mouse  1757 SLNS-----IHSSPGPKRST--NTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKP 1814

  Fly  1903 G 1903
            |
Mouse  1815 G 1815

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	42/189 (22%)
		RhoGEF	1353..1524	CDD:459876	68/172 (40%)
		PH_puratrophin-1	1524..1663	CDD:270062	41/143 (29%)
Kalrn	XP_011244253.1	PH1_Kalirin_Trio_like	1469..1591	CDD:270060	43/160 (27%)
		SH3_Kalirin_1	1657..1716	CDD:212786	12/61 (20%)
		SH3-RhoG_link	1714..1896	CDD:465196	22/118 (19%)
		RhoGEF	1939..2108	CDD:459876
		PH2_Kalirin_Trio_p63RhoGEF	2113..2247	CDD:270061
		SH3_Kalirin_2	2331..2387	CDD:212787
		I-set	2478..2572	CDD:400151
		Ig strand B	2495..2499	CDD:409353
		Ig strand C	2508..2512	CDD:409353
		Ig strand E	2538..2542	CDD:409353
		Ig strand F	2552..2557	CDD:409353
		Ig strand G	2565..2568	CDD:409353
		FN3	2576..2667	CDD:238020
		STKc_Kalirin_C	2697..2944	CDD:271017
		SEC14	47..185	CDD:214706	4/10 (40%)
		SPEC	198..418	CDD:238103	46/251 (18%)
		SPEC	319..544	CDD:238103	51/262 (19%)
		SPEC	545..774	CDD:238103	44/265 (17%)
		SPEC	899..1137	CDD:238103	48/249 (19%)
		SPEC	1143..1243	CDD:197544	11/101 (11%)
		RhoGEF	1292..1462	CDD:238091	68/180 (38%)