Protein Alignment Pura and RtGEF

DIOPT Version :9

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: NP_001097184.2
• Gene: RtGEF / 35306
• FlyBaseID: FBgn0015803
• Length: 1310
• Species: Drosophila melanogaster
• Alignment Length: 1229
• Identity: 228/1229 - (18%)
• Similarity: 372/1229 - (30%)
• Gaps: 483/1229 - (39%)

  Fly   857 FISVDQ-VTTSVAPSQLP-AGLLP---------------SVPALSTLPGISQRHHDATKWREFFA 904
            |:||.| ::..:...:|| .|::.               |.|.:..:..:.|..::|.||.|.
  Fly   307 FLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVEL-- 369

  Fly   905 QLEA--------FQRQCSTAGGRLVSALSEIRAA------DLQGLPTRRQLYGQH-------RAL 948
             |.|        .:||.|....   |:|..:.||      |.:|..||..|...:       |.|
  Fly   370 -LNANNPSLPMGIKRQLSNLSN---SSLGHLNAAHLSQHLDSRGYCTRFSLCAYYSSPPCHVRPL 430

  Fly   949 SRALMDSQLHSLRKMG--AGQLARL---QDLARAITSAPGSPRMTSQPQSSSSSSSGAFLNVDAA 1008
            ...|..|...:.....  :...|||   ..|..||.      :|...||:..|        :|
  Fly   431 RVTLPPSNYPATAPYANLSAHFARLVKGGGLRSAIV------KMLLYPQARQS--------ID-- 479

  Fly  1009 CKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALE----------DEISEVTSWMGSDG 1063
              |:::.|......:|:.:|:.|...:.....:|.||.|:|          |...:......||.
  Fly   480 --LKRIALRKRRCHKASAKLKDLNTNQDSGQSELERQDAIELPTDSESYDDDFEDDFLHSCDSDP 542

  Fly  1064 AENLQKF---------------------SQLHWDNESQLRTQEQEFEKYYFIAMKHLAK------ 1101
            .|.:|.:                     ::.|..:.:.::....:.::  .:|:..|.|
  Fly   543 FEYVQFYQNKRNDSMCNSTGTFVDHGTGARRHCSSINLIKLDSADTDE--VLALNELKKESLVIG 605

  Fly  1102 GRDLHAAALKVSVLSESANNLKSALD-----------------QFAQKLELAHERYDA----AGR 1145
            .|.|.|.|.|.:..:.|.:...:.|:                 :......|..:..||    |.|
  Fly   606 SRALRALARKSTTRNSSVHTSTATLELGVGGSITNCVEEEPILKVKPSFSLQQQSSDASSIFAAR 670

  Fly  1146 LLHLLTQHQRENAAREELQRLAEQLG-ATSLLEKHWPVMRKTHTLPVA----SSTPAAAAGKRVS 1205
            |....|       |.|.|..:.:.|. .:|:.|...|       ||.:    .|.|....|.|.:
  Fly   671 LGGAFT-------ACENLASMPDDLSRESSIQEPPTP-------LPASPTERHSMPTIFVGNRFN 721

  Fly  1206 YRCSSASRSSFEGGPSSSNCSCWRESRNLEDMDQMEEEEEEQDHDCDGGDGEEQQSKIADSGLGV 1270
            :     |:::....|:      ||            :.:|.|:...|....||..|...|..
  Fly   722 H-----SKNTEVYVPT------WR------------DRQEMQNQSVDAKQDEELHSSSIDLP--- 760

  Fly  1271 CDNCERNPKLARICSCQSLNEKSHDELL--EDECFDRP-----------------SKRYMDIMHS 1316
                         .:|.|..:|...|||  .||..::|                 .||......|
  Fly   761 -------------AACLSAPDKLQAELLYNYDEILEKPLQLHRELTPFPGHNLNSDKRVSHKSDS 812

  Fly  1317 PMEANA----HLQCHGSSLELPKLEELSCLDPKIQKTLLLIMREMIGTERDYVRSL----YYVIE 1373
            |...||    :|....||     ..|| |:|...:|......|     .||.:|..    :..:.
  Fly   813 PSTGNAKTDPNLATRSSS-----TTEL-CIDTTSKKRTTPSER-----SRDSIRRCISYQFLQMS 866

  Fly  1374 N--------------YIDELLR----EDIPQPLRGQRNVIFG----------NIEKIFEF----H 1406
            |              ::|...|    ...|.| |...:.:.|          |.|..|..    |
  Fly   867 NRPPPPPPPRRDPDLHLDTKCRCCENSQCPSP-RSSDSGMAGSCTITSPDPPNPESYFPMEAAGH 930

  Fly  1407 NSHFLGELERYE---------------------------------------RNPLKVGAAFLEME 1432
            :.....|.||::                                       ..|..:.:|.:::.
  Fly   931 DMLDNVEPERFDVCGMFREKFLTPEATQDVVDLSEEQPQLSDEPTTPTNRKEEPTCITSAQVQVN 995

  Fly  1433 SKFYLYALY----------NKNKPKSDTLLSEYGSSFFKPKQMQLQDKLDLASYLLKPVQRMGKY 1487
            ::    :::          |:|.|.::.|.|...:...:|:          |::      |.|.|
  Fly   996 TR----SIFLPSSSSMDETNRNVPANNILFSSSSADQLEPQ----------ATF------RSGMY 1040

  Fly  1488 A------LLLQQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNV 1546
            |      .|..::|   :|:..|                        :...|.:.||
  Fly  1041 AHWWKKERLPPEVV---RGIAHA------------------------YNKSLPSKDS-------- 1070

  Fly  1547 KEQGRLLRQNEFLVWQGRGGKKTLRQVFLFEELVLF-SKARRFPDHKNLDIYIYKNSIKTS---- 1606
            |:.|.:.  :......|..|         :.|..|: |..:...|:.|..:....|:..|:
  Fly  1071 KDSGSVC--SSCFCSLGASG---------YSEGALYCSVCQNCADYYNGSVTSTTNTTTTTSSAS 1124

  Fly  1607 ------DIGLTAHTGDSATKFEIWFRKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREV 1665
                  |.|:.|.|.||::                |.|   .|.|.
  Fly  1125 CPLCSEDEGMIAPTHDSSS----------------LDC---PICNG------------------- 1151

  Fly  1666 RLAEMSSMGIGSKPCLDIRPSNNQISDRSIPLAQLNKTPKLRHSE--PGKGSMRR---------- 1718
            |:|..:..|..|:  :|.||....|...: |.|   :.|...||:  ||:...||
  Fly  1152 RIASGAEEGKQSE--MDRRPDVAAIKPHT-PTA---RRPSASHSQQHPGRQQQRREPQPPGVSGH 1210

  Fly  1719 -------------PNSLISESSLSSGTSTTSGSSISGGSSSGHNDGGGRHSLHFGKLPGAHSASS 1770
                         |.|.:......|.::|:|.|:.:  :.|..:.||.|..:.|........:||
  Fly  1211 QLDVASQTEPVVQPPSQLQSVHAHSASTTSSASTTT--TKSAKSSGGIRPQIKFSPDTKQQDSSS 1273

  Fly  1771 TNCSATLELINETQALKLAKSPSGGGIKGADTAADG-GNTSGNGNGNGNAFRSSKRNHK 1828
            |                      .|.|.|..::..| |::||...|||.|.| .:|.||
  Fly  1274 T----------------------PGVIHGRQSSNSGSGSSSGGSGGNGGAKR-KERKHK 1309

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	PX_domain	15..>52	CDD:295365
		RhoGEF	1353..1532	CDD:279015	35/269 (13%)
		PH_puratrophin-1	1524..1663	CDD:270062	24/149 (16%)
		PH	1562..1655	CDD:278594	19/103 (18%)
RtGEF	NP_001097184.2	SH3_PIX	6..58	CDD:212810
		RhoGEF	79..249	CDD:238091
		PH_Cool_Pix	281..376	CDD:269932	16/71 (23%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45462286
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	2	1.840