DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pura and plekhg4

DIOPT Version :9

Sequence 1:	NP_996016.2	Gene:	Pura / 2768948	FlyBaseID:	FBgn0035802	Length:	1904	Species:	Drosophila melanogaster
Sequence 2:	XP_009301619.1	Gene:	plekhg4 / 326099	ZFINID:	ZDB-GENE-030131-4824	Length:	2630	Species:	Danio rerio

Alignment Length:	2148	Identity:	531/2148 - (24%)
Similarity:	847/2148 - (39%)	Gaps:	626/2148 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly    86 SDTQIDVPGGGIPGGQIPSLESTDRDMKEARAAPIARDDEDQEAGDPEEHGEEEDEEPPEVEQLS 150
            |.||:|           ..|.||.:|.:|:::...:.|:|..:.       .:.|.||.:..|..
Zfish   760 SPTQLD-----------SKLVSTLQDKQESKSVNNSIDNEPVKT-------SQADTEPLKRSQFD 806

  Fly   151 ANANYVCCRYPGTTSSSTSGAADTL--------------NRSKSRVQKYLKKCKQRLTG------ 195
            :|.         .::|...|...::              |:|.|..|         |.|
Zfish   807 SNP---------VSNSQLDGKQVSISQLVNEIFSTPQPDNKSVSTTQ---------LDGNIDRTS 853

  Fly   196 --QAKMPTSSSTTTTTITLI-RNEPAPTTIAERES-------EQALAEQEEELLEVE--DL---- 244
              .:|..:.|.|.:..:::. .|||..|:..:.||       :.::::...|.::..  ||
Zfish   854 QLDSKQESVSQTDSKPVSISPDNEPVKTSQPQLESTLHPNSKQASMSQLVNEHIQTSQPDLKPVS 918

  Fly   245 ------TEGSNGSYTDALPIECSLSASIAEEQQQGVDELEPPKEEEVPLETELTE---------- 293
                  .:.....|.|..||...|...:....|.....::.|:.|...:...|.|
Zfish   919 IAQLYSEDAKPDGYADVKPITTELDNGLVSTSQPDNQPVDTPQAECTTVNITLPEGNPNDTSYIV 983

  Fly   294 --PSSTPPPQS----------NPEGIPAPERFRLTADVDATLARLIDSHLSHIYPVY-------- 338
              |.||..|.|          |......||    |..:...|.|   .:...:.|.|
Zfish   984 NLPLSTSQPNSKTVHVEQADINVINTSQPE----TEMLSCVLER---ENSEQVSPAYIELIVSDP 1041

  Fly   339 WARTRAILIQQARE-RLVCGFDGSLVRFEQRFLCKFAQ-IAATLRTAHQIGESW--LWHPGWPLA 399
            :|..|.    :::| |:.|..:||......|  ...|: :|.::.:.:||.::.  |:....|:.
Zfish  1042 YAEVRC----ESKELRVNCDLEGSCGSQNNR--ASLAETVAESVSSQNQIDQTGNSLYQIHKPIH 1100

  Fly   400 TSNGALVLQLSDAEIAHAL----RPSELYLCIKLESTESDADCKPRLYAVWRSSRKEESVQSHCI 460
            |          .:|..|.|    ...:.:......:.||..|..|                   |
Zfish  1101 T----------PSEQMHDLPTIKEDHQTFSSSSHNTNESVGDLSP-------------------I 1136

  Fly   461 DHENPGELPLSVLQLEMSTAAAALMASDCGELP-QLLQNLLVSVERSIERVSLNELQMPMHHDRG 524
            ..|:.|.:..::....::..:...:.   ||.| .|:....|..|...:|::..|:::....:|.
Zfish  1137 AEEDGGSVTAALYSEHVALVSVTTVP---GESPVTLISQGQVGTESPEDRINQTEVELQTKEERA 1198

  Fly   525 EDSLDEANNNGCNNNLLGSPKCALRRSDLITKNKLFNSRYMLRRLTN------RQDSMNSTSSGN 583
            .            .|:.|.|.|  :.::::.|..  :|...::...|      ||:.:.::.:|.
Zfish  1199 V------------QNVAGMPPC--KGAEMVEKKS--SSEAEIKTDENDVVAVCRQEEVETSQTGL 1247

  Fly   584 SNVSDRSS---SSSSSSSSSAGEELLTS-----------------HSEAKMRSNSVGNEASGAST 628
            .:.:.:|.   ....||.....|:..||                 ..||.:.||| .|....|.:
Zfish  1248 VDATPKSDVQVQEEVSSPVETDEKKQTSVQEKGVEEEEVKAKTVREQEAIVTSNS-ENRVQSACS 1311

  Fly   629 SPALPLYCLGNSFPHI---------------------------DSDEEASDSIGPGKHHSLE--- 663
            ....|...:.|:..|:                           ..||.||...|..|.|..:
Zfish  1312 EVLDPASDVPNTNGHMHAQDTPTTELKGQITKEEDGKGAEEKRQEDEIASSVNGSQKEHQQQADM 1376

  Fly   664 -----------------------TCETE-----LLPS-------------SSIAELPEKLLTSGV 687
                                   :.:||     .:||             |...::...:|:|||
Zfish  1377 DSSFHQQEEQACSQKVDSTVCHLSAKTESEDPSAVPSPQPPVDHRAQPVKSKAHDINPDVLSSGV 1441

  Fly   688 Y-LPGTRTLQGDPLVTVDAACVASAGINCY--EMATLLLYY-STIPERSSCPFTTPATPGTTATI 748
            . |.|||...|..||||..........||.  |:..:::|: ||:.:..|       ..|.|..:
Zfish  1442 LCLTGTRDKSGHALVTVTMRNTVWLNPNCNSGELVRIMVYFCSTLRKEVS-------ALGLTVLV 1499

  Fly   749 NGHKSTEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQIGNCEILAV 813
            :..:.:                |....|.....::::...|:..::..:.|.||.::
Zfish  1500 DARRCS----------------PVPALFKAFNILQEAMPGCIHTVLLLADRDLALRM-------- 1540

  Fly   814 CPDSSLLQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVKFISVDQVTTSVAPSQLPAGLLP 878
                                           |.:|...|:|:  .|:..:...|..||||.....
Zfish  1541 -------------------------------EKTSSIQVELL--TSLKSLYKHVDVSQLPTEFEG 1572

  Fly   879 SVPALSTLPGISQRHHDATKWREFFAQLEAFQRQCSTAGGRLVSALSEIRAADLQGLPTRRQ--- 940
            :.|            :..:.|..|..:||.....|..|...|.:.:|::.:..|.  ||..:
Zfish  1573 TFP------------YAHSSWVSFRMRLEQLTSHCEDAVSLLQNTISKLESTVLP--PTAEEAQL 1623

  Fly   941 LYGQHRALSRALM-DSQLHSLRKMGAGQLARLQDLARAITSAPGSPRMTSQPQSSSSSSSGAFLN 1004
            |..::|.|.|.:: ||:|..|:..|...|:||:                 :.::|.|      |.
Zfish  1624 LLCKYRELMRTVLEDSRLVRLQLEGGAALSRLR-----------------KEETSVS------LT 1665

  Fly  1005 VDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALEDEISEVTSWMGSDGAENLQK 1069
            .|....:.....|:|:||....||..|:.:..:.|..:...::||:...||:.|:...|...||.
Zfish  1666 EDYRDAIESAGRLYNQVDELVHRLVMLSNKCTQELEFIMEFKSLEEGFKEVSQWIEEVGESRLQT 1730

  Fly  1070 FSQLHWDNESQLRTQEQEFEKYYFIAMKH----------LAKGRDLHAAALKVSVL--------- 1115
            .|:|. |:..||..::..|.::|..|.:|          |.|..|:.:|.|:|..:
Zfish  1731 LSELE-DSLEQLHHKQSIFREFYTAAYEHCKSGEALLKRLEKWEDVSSAELQVYEVKVRSFWVHL 1794

  Fly  1116 ---SESANNLKSALDQFAQKLELAHERYD-AAGRLLHLL------------------------TQ 1152
               |:...:.|..:|:..|..|...:.|: |...:.||.                        :|
Zfish  1795 RDFSQRVEDTKDKIDKTVQLYEFFDKAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQ 1859

  Fly  1153 HQR-ENAAREELQRLAEQLGATSLLEK---HWPVMRKTHTL------------------------ 1189
            |.. .:|..:|::.||.:|.:...|::   .|...::|..:
Zfish  1860 HPPIPDAHFQEMKDLAGELKSEQGLKQWKFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDS 1924

  Fly  1190 --------------------------------------PVASSTPAAAAGKRV----SYRCSSAS 1212
                                                  ...||||::.:|.|:    :.|..:.|
Zfish  1925 SRRNSDVSTKHQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGS 1989

  Fly  1213 RSSFEGG-PSSSNCSCWRESRNLEDMDQMEEEEEEQDHDCDGGDGEEQQSKIADSGLGV------ 1270
            ..|.|.. |.|:.....|.:|::.     .:|..:..|       .|..:..:.||..|
Zfish  1990 PYSIEHRIPHSTPVRSHRLTRSIS-----TDESPQSPH-------AEGLASTSPSGTSVQTNRRV 2042

  Fly  1271 ------CDNCERNPKLARICSCQ-SLNEKSHD----------ELLEDECFDRPSKRYMDIMHSPM 1318
                  .|.....| :::..:|| :|:|.:..          |:...|..|||....:..:|...
Zfish  2043 LRKTQSFDTAGSEP-VSQYGTCQRTLSEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWS 2106

  Fly  1319 EANAHLQCHGSSLELPKLEELSCLDPKIQKTLLLIMREMIGTERDYVRSLYYVIENYIDELLRED 1383
            ..:||..  ||    |..|..|    |..| |..|:.||:.|||:|||||.|:|:||..|:.|..
Zfish  2107 SIDAHRS--GS----PAPETRS----KGSK-LRHIVDEMVTTEREYVRSLRYIIDNYFPEMERAV 2160

  Fly  1384 IPQPLRGQRNVIFGNIEKIFEFHNSHFLGELERYERNPLKVGAAFLEMESKFYLYALYNKNKPKS 1448
            :||.|||:|:|||||:||:.:||:.:||.|||....:||:|...||..:.:|.|||||:||||||
Zfish  2161 LPQDLRGKRSVIFGNLEKLVDFHSQYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKS 2225

  Fly  1449 DTLLSEYGSSFFKPKQMQLQDKLDLASYLLKPVQRMGKYALLLQQLVKACKGVEGAALQEIAADV 1513
            ||||:.:|::|||.|||:|.||:|||||||||:|||.||||||:.|:|     |.:.:||  .::
Zfish  2226 DTLLASHGNNFFKHKQMELGDKMDLASYLLKPIQRMSKYALLLKDLIK-----EVSEVQE--QEL 2283

  Fly  1514 EELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNVKEQGRLLRQNEFLVWQGRGGKKTLRQVFLFEE 1578
            ..|:.|.|||||||||||||||||::||||||:||||:|:||:||.:..||  ||..|.|||||:
Zfish  2284 TYLRAAAEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTISYGR--KKCQRHVFLFED 2346

  Fly  1579 LVLFSKARRFPDHKNLDIYIYKNSIKTSDIGLTAHTGDSATKFEIWFRKR-KPDDTWMLQCMSED 1642
            ||||||.:|.  ...||:||||:|.||:|:|:|..:|:::.:||||||:| ..:.|::||..:.|
Zfish  2347 LVLFSKPKRI--EGGLDVYIYKHSFKTADVGMTETSGENSLRFEIWFRRRTSKNQTYILQSSTAD 2409

  Fly  1643 IKNAWTEEISKLLWKQAKRNREVRLAEMSSMGIGSKPCLDIRPSNNQISDRSIPLAQLNKTPKLR 1707
            ||:|||.:|:::||:||.||:|:|:.||.|||:|:||.|||:||:..|:||:|......:..:.|
Zfish  2410 IKHAWTCDIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTR 2474

  Fly  1708 ---------HSEPGK-GSMRRPNSLISESSLSSGTSTTSGSSISGGSSSGHNDGGGRHSLHFGKL 1762
                     ||.|.| .::..|               .||:.:|||.||         |...|.|
Zfish  2475 ASIAVSLFDHSNPFKRAAVNTP---------------VSGTPVSGGPSS---------STLLGPL 2515

  Fly  1763 PGAHSASSTN-----------CSATLELINETQALKLAKSPSGGGIKGADTAADGGNTSGNGNGN 1816
             ..|..||.:           |....||.:||.:.....:.|.|   .:.....|..:||:.:|.
Zfish  2516 -NLHMYSSQSLLAGERPLIGPCIEEDELEHETSSQPSMTTESSG---SSSHCLSGSGSSGSDSGC 2576

  Fly  1817 GNAFRSSKRNHKRSTTIVSQLSMESGIVSDMCVTPDQEQSAAESSRSSWSTSTTGASGASTTSAS 1881
            .::......:.:.|:...|  |..|.|.|     |.||:....|...|       |..|.....|
Zfish  2577 VSSHLPEALSEEPSSPCDS--SCYSSITS-----PTQEKPCFNSQYIS-------AGDAQVIGPS 2627

  Fly  1882 TVI 1884
            |::
Zfish  2628 TIV 2630

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	PX_domain	15..>52	CDD:295365
		RhoGEF	1353..1532	CDD:279015	103/178 (58%)
		PH_puratrophin-1	1524..1663	CDD:270062	81/139 (58%)
		PH	1562..1655	CDD:278594	47/93 (51%)
plekhg4	XP_009301619.1	COG5099	467..>912	CDD:227430	34/187 (18%)
		SEC14	1433..1573	CDD:238099	38/203 (19%)
		BAR	<1707..>1807	CDD:299863	25/100 (25%)
		RhoGEF	2130..2302	CDD:279015	103/178 (58%)
		PH_puratrophin-1	2294..2430	CDD:270062	81/139 (58%)
		PH	2319..2414	CDD:278594	50/98 (51%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	227	1.000	Domainoid score	I2440
eggNOG	1	0.900	-	-		E1_KOG0689
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	488	1.000	Inparanoid score	I1385
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D263447at33208
OrthoFinder	1	1.000	-	-		FOG0005689
OrthoInspector	1	1.000	-	-		otm24842
orthoMCL	1	0.900	-	-		OOG6_106140
Panther	1	1.100	-	-	LDO	PTHR45845
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1908
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	11	10.830