Protein Alignment Pura and quob

DIOPT Version :10

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: XP_068077749.1
• Gene: quob / 321031
• ZFINID: ZDB-GENE-030131-9944
• Length: 1988
• Species: Danio rerio
• Alignment Length: 2034
• Identity: 487/2034 - (23%)
• Similarity: 761/2034 - (37%)
• Gaps: 609/2034 - (29%)

  Fly   234 QEEELLE-----------VEDLTEGSNGSYTDALPIECSLSASIAEEQQQGVDELEPPKEEEVPL 287
            :|..:||           :.::.||..|:...    .|.||:      .:||        .:||.
Zfish   151 EETPILETSYPCIFTESWLREINEGRQGNRLS----RCVLSS------DKGV--------VKVPW 197

  Fly   288 ETELTEPSSTPPPQSN--PEGIPAPERFRLTADVDATLARLIDSHLSHIYPVYWARTRAILIQQA 350
             ||:..|.....|:||  |:..|.........|::..::.:....:....|:           :.
Zfish   198 -TEVANPEFLDRPKSNVWPDSKPCSTATIRVRDLNTEISDIESKVVVDFLPL-----------EM 250

  Fly   351 RERLVCGFDGSLVRFEQRFLCKFAQIAATLRTAHQIGESWLWHP-GWPLATS---------NGAL 405
            ..|:|...||..:..:   |.:.......:..||.| .|:...| ||....:         .|..
Zfish   251 ETRIVPAIDGLALSVQ---LVESGSRLVKVNQAHTI-SSFAGKPVGWVSPNTWDSRQTQELEGEY 311

  Fly   406 VLQLSDAEIAHALRPSELYLCIKLESTESDADCKPRLYAVWRSSR-KEESVQSHCIDHENPGELP 469
            |..|...:....|.|:::.:           ..||..:.:.|::: ..:|..|.|....||.:.|
Zfish   312 VDLLEFNKEKETLVPNKIAM-----------PMKPLGFKLVRTAQPSSKSNVSICGVDCNPNKQP 365

  Fly   470 LSVLQLEMSTAAAALMASDCGELPQLLQNLLVSVERSI--ERVSLNELQMPMHHDRGEDSLDEAN 532
            ..::|.:..        |.|    :..|:.:.::...:  ||.|:    :|        .|||..
Zfish   366 SEIMQHDPD--------SKC----RYRQSYMAALRNPVNFERASM----LP--------CLDETR 406

  Fly   533 NNGCNNNLLGSPKC-------ALRRSDLI---TKNKLFNSRYMLRRLTN----RQDSMNSTSSGN 583
            .:...    ..|.|       .|:.|..:   |.|:.  .|..::...|    :|...|||....
Zfish   407 TDAGK----AEPSCEGYGVGLRLQPSSCMFGQTPNQA--DRNPMQSFENSAQQKQSPKNSTQPSQ 465

  Fly   584 SNVSDRSSSSSSSSSSSAGEELLTSHSEAKMRSNS------------VGNEASGASTSPALPLYC 636
            :...||.|..::.|......:|:||..:|:....|            :|.:|......|     |
Zfish   466 NVPHDRKSRENADSPDQHMTQLITSMVQARQHHKSFLTGQPDLPQTVLGTKALPDKRHP-----C 525

  Fly   637 LGNSF------PHIDSDEEASDSIGP-----------GKHHSLETC----------------ETE 668
            |.|:.      ..|...:.|.....|           |..|....|                .||
Zfish   526 LNNAIRQDSFHMRISGQKHAKTQHLPKMGLRALPDHGGHRHDANLCLQGFSPIQHSQNQPTHITE 590

  Fly   669 LLPSSSIAELP----EKLLTSGVYL--------PGT--RTLQGDPLVTVDAACVASAGINCYEMA 719
            ..|...|::.|    ..||...|:.        ||:  :.::|      .:...:.:.::..|.|
Zfish   591 QTPQPQISQNPFRNENSLLDCNVFCDDRHCGRGPGSPVQVIKG------KSRSKSRSSLSVSETA 649

  Fly   720 TLLLYYSTIPERSSC---PFTTPATPGT-----TATINGHKSTEQQQQQQQ-------------- 762
            ...|..:....||..   |...|..|..     ||.:...|....|:.:::
Zfish   650 RQCLQSNKPTNRSHSDVYPEIIPMLPAIHGIKHTADLVSPKLNRPQEARKEVSLSGKAGAPVVNG 714

  Fly   763 ---QQQQQKQQPAQ-QTFTILIAIEKSQ-----HLCVIDLIC----------------------- 795
               |:.|...||.. |:.|.:..|..||     .|..:.:||
Zfish   715 VELQKLQHSDQPTTGQSVTEIQGIYISQDPTIKSLLQLGIICLPGGRDRAGRAVVEVYGGRNGWI 779

  Fly   796 -------QSLRLL----------AKQIGNCEILAVCPDSSLLQLCNGQQQQKLQQQQQ------- 836
                   :..|||          .:::|    |.|..||....|.:...:..|..|:|
Zfish   780 SPQVSPLELCRLLLYLHSIPRSEVRELG----LTVVIDSRKCPLTSVFYKSLLMVQEQALHAVHT 840

  Fly   837 -----QNNNSDHPEPSSPSPVDLVKFISVDQVTTSVAPSQLPAGLLPSVPALSTLPGISQRHHDA 896
                 ..:.:..||......:|:|  .|:..:..:|...||.:.|..|.|         ..|.|
Zfish   841 ILMLVDKDANPRPEKHPGLQIDVV--TSLKALHKTVDGHQLTSDLSGSFP---------YSHED- 893

  Fly   897 TKWREFFAQLEAFQRQCSTAGGRLVSALSEIRAADLQGLPTRRQLYG----QHRALSRALMDSQL 957
              |.:|..:|..||.:...|...|.:|   ||..|::...|.:.:..    |..::...|.|::|
Zfish   894 --WLQFHQKLSLFQIELEAASSLLHTA---IRRLDVKRTDTAKDVQSCIQEQRSSMKMVLEDTRL 953

  Fly   958 HSLRKMGAGQLARLQDLARAITSAPGSPRMTSQPQSSSSSSSGAFLNVDAACKLRKVTLLFNEVD 1022
            .:|::.|...|||::                 :.:|..:.|.      |....|..||.|:::|:
Zfish   954 VALQREGGAILARMR-----------------REESRFAHSE------DFRDSLESVTCLYDQVE 995

  Fly  1023 RAAQRLEQLTEQRRERLRQLTRQRALEDEISEVTSWMGS----------DGAENLQKFSQLHWDN 1077
            .:...|...:.|..:.|..:...|..:|.:..:..|..:          |..:.|||..|...|.
Zfish   996 ESVHTLVMKSNQSLQHLEHVLLLRETQDSLHTIQKWCDAEERLWQKDKDDAEKTLQKIQQRLQDT 1060

  Fly  1078 ESQLRTQEQEFEKYYFIAMKHLAKGRDLH----------------------------AAALKVSV 1114
            ::.||..::..|:...:......|.:..|                            .:.|:.||
Zfish  1061 QTLLRQAKENKERGMLLIKDVERKSKGTHYPETDAFRCYTTGFKTRMTGFLLKAEERKSDLQTSV 1125

  Fly  1115 ----LSESANNLKSALDQFAQKLELAHERYDAA--------GRLLHLLTQHQRENAA-------- 1159
                ..|.|::|.....::...::.::......        .|..|...||.::.:|
Zfish  1126 DLYRFCEEASSLVKECMEYLDHMKSSNSPMSVCWSSLHAFEQRFQHFSPQHFQDRSAALLERLPD 1190

  Fly  1160 ------------REELQRLAEQLGATSLLEKHWPVMRKTHTLPVASSTPAAAA----GKRVSYRC 1208
                        |:..|||:|.|.|.   ..|.....:....||:..|...||    .:.::| .
Zfish  1191 GIWMWNAVQTQCRDIRQRLSEILQAA---HTHKDSQDEAQLNPVSGQTQNRAAEQGDNETLTY-L 1251

  Fly  1209 SSASRSSFEGGPSSS-----------NCSCW-RESRNL-EDMDQMEEEEEEQDHDCDGGDGEEQQ 1260
            .||..|.||...:||           :..|. :|:|:| |..|::.......:..........|.
Zfish  1252 QSAEGSKFEQVMTSSVHEQSASRIQQSVECTEQETRHLHETKDKLHNASSMHNESTSHAQQSVQC 1316

  Fly  1261 SKIADSGLGVCDNCERNPKLAR----------ICSCQSLNEKSHDELLEDECFDRPSKRYM---- 1311
            :....|.|...:|..|||.:.:          .|:.|:.:.....:.:.....||..|.:.
Zfish  1317 TVQKASMLQETNNTSRNPSMHKDSASHIQESVQCTDQNTSHLHEPQAISHHPSDRKLKEHHHKHS 1381

  Fly  1312 ----DIMHS--------------------------------PM-EANAHLQCHGSSLE------- 1332
                |:..|                                |: :.:|...|.|:|||
Zfish  1382 HSDDDLRKSGRIHKFGQTAHYKVFIRSQSAESCLRGYRYNIPLCKTDAAASCVGASLEGLSSELN 1446

  Fly  1333 -----------------------------------LPKLEELSCLDP----------KIQKTLLL 1352
                                               ..|.||..|...          |:|:
Zfish  1447 QHDSFCSSASGFSQKCSSDCGSEDHGSGVRCSDNAFTKQEESVCKPECILENTSTALKLQR---- 1507

  Fly  1353 IMREMIGTERDYVRSLYYVIENYIDELLREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELERY 1417
            ||:|::.||::|||:|.||:|||:.||.|.|:||.|||||.:||||:||:.:||..||:.|||..
Zfish  1508 IMQELLQTEQEYVRALAYVVENYMPELERPDVPQDLRGQRGLIFGNLEKLRDFHQHHFIQELELC 1572

  Fly  1418 ERNPLKVGAAFLEMESKFYLYALYNKNKPKSDTLLSEYGSSFFKPKQMQLQDKLDLASYLLKPVQ 1482
            ...|..||..||:.:..|.|||||:||||:|:.||.::|..|||.||..|:|.|||:||||||||
Zfish  1573 LEKPFSVGRCFLKHKESFGLYALYSKNKPRSENLLIQHGKDFFKQKQQLLKDALDLSSYLLKPVQ 1637

  Fly  1483 RMGKYALLLQQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNVK 1547
            |:.||:||||.|::.|:.|..|.||.     .|:..|..:::|||||||:|||||.:..||||:|
Zfish  1638 RISKYSLLLQDLLRECECVTNAELQR-----TEIHTALNIIQFQLRHGNNLLAMDDIYGCDVNLK 1697

  Fly  1548 EQGRLLRQNEFLVWQGRGGKKTLRQVFLFEELVLFSKARRFPDHKNLDIYIYKNSIKTSDIGLTA 1612
            |||:|:||:|||:...:  ||..|.:|||::|:||||.|: .|..| |.||||.|.||||||:|.
Zfish  1698 EQGQLIRQDEFLLTFRK--KKCYRHIFLFQDLILFSKTRK-TDVGN-DTYIYKQSFKTSDIGMTH 1758

  Fly  1613 HTGDSATKFEIWFRKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREVRLAEMSSMGIGS 1677
            ::|||...||||||:||..||::||..|.::|.:||.::.::||:||..|||:|:.|...||||.
Zfish  1759 NSGDSGLCFEIWFRRRKTQDTYVLQAASREVKESWTRDLERILWEQAIHNREIRMQERVFMGIGH 1823

  Fly  1678 KPCLDIRPSNNQISDRSIPLAQLNKTPKLRHSEPGKGSMRRPNSLISESSLSSGTSTTSGSSISG 1742
            ||.:||:||...||||:|......:         |..|:.|.||:.|.|..||..|.:|.||..|
Zfish  1824 KPFMDIKPSEMAISDRAINCVLTGR---------GGVSVLRLNSIGSASGTSSSGSQSSCSSGWG 1879

  Fly  1743 GSSS------GHND------------------GGGRHSL------HFGKLPGAHSASSTNCSATL 1777
            ..|:      |.:|                  ..|.||.      ..|:.....|.|:.:||:.:
Zfish  1880 SPSTSAVCPVGGDDVRYIQETSGILEEDDLDQESGNHSTLVASSESSGESVSCFSTSAHSCSSVI 1944

  Fly  1778 ELINETQALKLAKSPSGGGIKGADTAADGGNTSGNGNGNGNAFRSSKRNHKRSTTIVSQLSMESG 1842
                                        ||:.....:..|   :..|::|.::|.
Zfish  1945 ----------------------------GGDCEHTPSELG---KQHKQDHLKTTN---------- 1968

  Fly  1843 IVSDMCVTPDQEQSAAESS 1861
                  .|.||..|..:||
Zfish  1969 ------QTRDQSGSKVQSS 1981

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	32/216 (15%)
		RhoGEF	1353..1524	CDD:459876	90/170 (53%)
		PH_puratrophin-1	1524..1663	CDD:270062	76/138 (55%)
quob	XP_068077749.1	RhoGEF	1508..1654	CDD:459876	83/145 (57%)
		PH_puratrophin-1	1674..1809	CDD:270062	76/138 (55%)