Protein Alignment Pura and Mcf2

DIOPT Version :10

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: XP_063136165.1
• Gene: Mcf2 / 317598
• RGDID: 1566098
• Length: 1203
• Species: Rattus norvegicus
• Alignment Length: 1335
• Identity: 301/1335 - (22%)
• Similarity: 506/1335 - (37%)
• Gaps: 360/1335 - (26%)

  Fly   612 AKMRSNSVGNEASG---ASTSPALPLYCLGNSFPHIDSDEEASDSIGPGKHHSLETCETELLPSS 673
            |:.||...|...:|   |.:.||          |.:.|...|..::......:..|.:.:|.|.
  Rat     2 AEARSPPTGPSPAGPPPAGSPPA----------PALASPWLAFRNLPKAASRAPRTRKRQLNPD- 55

  Fly   674 SIAELPEKLLTSGVYLPGTRTLQGDPLVTVDAA-----------------CVASA---------- 711
                       :|.::|.|:|:.    |.||.|                 |:..|
  Rat    56 -----------NGTFVPFTQTIP----VQVDMAMALHRVRIAEKYGKNYLCLLQAVFLAETGLLL 105

  Fly   712 ---GINCYEMATLLL----------YYSTIPERSSCPFTTPATPGTTATINGHKSTEQQQQQQQQ 763
               .||.:.|..:..          :..|.||  :|.|...........:|...|.
  Rat   106 QLKDINHFLMQDIAFLSGGRGRDNAWIITFPE--NCNFRCIPEEVIAKVLNYLTSI--------- 159

  Fly   764 QQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQI-----GNCEILAVCPDSSLLQLC 823
               .:|..:...|||::.....        ...||::..::|     ||..::.|...:|.||..
  Rat   160 ---ARQSGSDSRFTIILDRRMD--------TWSSLKISLQKISASFPGNLHLVLVLRPTSFLQRT 213

  Fly   824 NGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVKFISVDQVTTSVAPSQLPAGLLPSVPALSTLPG 888
            ......:..|:...            ..:.:|...||..:.|.:...||       .|.|.   |
  Rat   214 FTDIGFRFSQEDFM------------LKLPVVMLSSVSDLLTYIDDKQL-------TPELG---G 256

  Fly   889 ISQRHHDATKWREFFAQLEAFQRQCSTAGGRLVSALSEIRAADL-QGLPTRRQLYGQHRALSRAL 952
            ..|..|  ::|..|...:|.|..........|.|..:|:...:| ..:|:..::.. .||....|
  Rat   257 TLQYCH--SEWIIFRNAIEKFAVTVKEMAQMLQSFGTELAETELPDDIPSIEEILA-GRAERYHL 318

  Fly   953 MDSQLHSLRKMGAGQLARLQ--DLARAITSAPGSP-----------RMTSQPQSSSSSSSGAF-- 1002
            :.:.|.::.|.|...|..||  |....::|:...|           ::.:|.....::..|.:
  Rat   319 LKNDLTTVTKEGKVLLMNLQVPDTEENLSSSHECPQHINGDWQTIKKLLAQVHDMETAFDGFWEK 383

  Fly  1003 --LNVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALEDEISEVTSWMGSDGAE 1065
              |.::...:|.|....|.||   ..::|.|..|:|              |:.:||..: :...:
  Rat   384 HQLKMEQYLQLWKFEQEFQEV---VTQVEFLLSQQR--------------ELGDVTGNL-AQVKQ 430

  Fly  1066 NLQKFSQLHWDNESQLRTQEQEFEKYYFIAMKHLAKGR--DLHAAALKVSVLSESANNLKSALDQ 1128
            .|:|...|  |::||                :.|||||  .||...|        |:|...|||.
  Rat   431 RLKKIEIL--DDKSQ----------------ELLAKGRIVILHGHKL--------ASNHHYALDL 469

  Fly  1129 FAQKL------------ELAHERYDAAGRL-LHLLTQHQRENA----------------AREELQ 1164
            ..|:.            |:..:|...:... :|.|.|..|:..                .:|:.|
  Rat   470 ICQRCNELRYLSDILVNEIRSKRIQLSRTFKMHRLLQQARQCCDQGECLLANRGMDKFQTKEDAQ 534

  Fly  1165 RLAEQLGATSLLEKHWP-----VMRKTHTLPVASSTPAAAAGKRVSYRCSSASRSSFEGGPSSSN 1224
            :..:.:  .:.|:...|     :........|..|....|..|.:..:..:. ||.||
  Rat   535 KALQDV--DNFLQMAMPFINYDIENLQFEFDVLLSPELKAQMKNIQRKLENI-RSVFE------- 589

  Fly  1225 CSCWRESRNLEDMDQMEEEEEEQDHDCDGGDGEEQQSKIADSGLGVCDNCERNPKLARICSCQSL 1289
                    |.::..::.::..|         |..|:...|...:........:||...|
  Rat   590 --------NQQNAYKISKDRPE---------GAVQRMIPAPESVMRARTIFFSPKHVGI------ 631

  Fly  1290 NEKSHDELLEDECFDRPSKRYMDIMHSPMEANAHLQCHGSSLEL-----PKLEELSCLDPKIQKT 1349
               .:......:.|.:..|.:..|.   .::|..:|....|.|.     |||:  |.||  |.|.
  Rat   632 ---GYSFFQACKIFSKVKKSWRQIQ---AQSNVKVQATQDSQEKNFDQPPKLD--SSLD--ILKN 686

  Fly  1350 LLLIMREMIGTERDYVRSLYYVIENYIDEL----LREDIPQPLRGQRNVIFGNIEKIFEFHNSHF 1410
              .::.|:|.|||.|||.|:.|:..|..|:    :.:.:|..||.:::|:|||:.:|:||||:.|
  Rat   687 --HVLNELIQTERAYVRELFTVLLGYRSEMDNPQMFDLMPPLLRNKKDVLFGNMAEIYEFHNNIF 749

  Fly  1411 LGELERYERNPLKVGAAFLEMESKFYLYALYNKNKPKSDTLLSEYGS-SFFKPKQMQLQDKLDLA 1474
            :..||.....|.:||..|||.:..|.:||.|.:|||:|:.:..:|.. :||:..|.:|:.:|.|.
  Rat   750 MSRLEDCSDAPERVGPCFLERKEDFQMYAKYCQNKPRSELIWRKYSECAFFQECQRKLKHRLGLD 814

  Fly  1475 SYLLKPVQRMGKYALLLQQLVKACKGVEGAA-LQEIAADVEELQRAEEMVKFQLRHGNDLLAMDS 1538
            |||||||||:.||.|||::|:|..|..||.. |:|....:.||          |:..||.:...:
  Rat   815 SYLLKPVQRITKYQLLLKELLKYSKEGEGTTKLKEALDSMLEL----------LKSVNDSMHQTA 869

  Fly  1539 LRDCDVNVKEQGRLLRQNEFLVWQG-RGG----------KKTLRQVFLFEELVLFSKARRFPDHK 1592
            :.....|:.:.|:::.|..|.||.| |.|          |...|.:||:|:.|:|.| |||...:
  Rat   870 INGYVGNMNDLGKMILQGAFSVWVGHRKGATKMKDFARFKPMQRHLFLYEKAVMFCK-RRFESGE 933

  Fly  1593 NLD---IYIYKNSIKTSDIGLTAHTGDSATKFEIWFRKRKPDDTWMLQCMSEDIKNAWTEEISKL 1654
            ..|   .|.:|:.:|..:.|:|.|......|||||:.::  ::.:::|..:.|:|..|.:||..:
  Rat   934 GADRYPSYSFKHCLKMEEAGITEHVKGDNRKFEIWYGEK--EEIYIVQAPNIDVKMLWLKEIRNI 996

  Fly  1655 LWKQAKRNREVRLAEMSSMGIGSKPCLDIRPSNNQISDR-SIPLAQLNKTPK----LRHSEPGKG 1714
            |.||                   :..|..:...:|:||: .:|..|.|...:    |...|.| .
  Rat   997 LLKQ-------------------QELLTAKTQQDQLSDQDQLPPQQQNAEKQEETLLNAVEAG-A 1041

  Fly  1715 SMRRPNSL--ISESSL-----------------SSGTSTTSG---SSISGGSSSGHNDG---GGR 1754
            .:.:.:::  :.|::|                 :.||:.|:.   :|:|..|.:|.:..   ..:
  Rat  1042 EVEQASAMMEVREAALLVQAEANCTTWNGMLPCTEGTAATAEHPCNSLSPNSDNGEDRSQMVSMK 1106

  Fly  1755 HSLHFGKLPGAHSASSTNCSAT--LELINETQALK 1787
            ..:|...||..      ||.|.  |.|::.||.|:
  Rat  1107 SFVHQNLLPNG------NCLAPKHLLLLHRTQKLQ 1135

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	37/177 (21%)
		RhoGEF	1353..1524	CDD:459876	71/176 (40%)
		PH_puratrophin-1	1524..1663	CDD:270062	44/152 (29%)
Mcf2	XP_063136165.1	None