DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pura and TIAM2

DIOPT Version :9

Sequence 1:	NP_996016.2	Gene:	Pura / 2768948	FlyBaseID:	FBgn0035802	Length:	1904	Species:	Drosophila melanogaster
Sequence 2:	NP_001371475.1	Gene:	TIAM2 / 26230	HGNCID:	11806	Length:	1701	Species:	Homo sapiens

Alignment Length:	1675	Identity:	329/1675 - (19%)
Similarity:	569/1675 - (33%)	Gaps:	560/1675 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly   533 NNGCNNNLLGSPKCA-----LRRSDLITKNKLFNSRYMLRRLTNRQDSMNSTSSGNSNVSDRSSS 592
            :||.|.:.:.:..|:     ||.|..:...            |:........||.:|..|.|.|.
Human   178 HNGGNPSKVPAEDCSEPVQLLRYSPTLASE------------TSPVPEARRGSSADSLPSHRPSP 230

  Fly   593 SSSSSSSSAGEELLTSHSEAKMRSNSVGN-----EASGASTSPALPLYCLGNSFPHIDSDEEASD 652
            :.|...||.|..|   .||:....:..||     ||.|:..:|.:|...|..|||..|:.:..:.
Human   231 TDSRLRSSKGSSL---SSESSWYDSPWGNAGELSEAEGSFLAPGMPDPSLHASFPPGDAKKPFNQ 292

  Fly   653 SIGPGKHHSLETCETELLPSSSIAEL-PEKLLTSGVYLPGT------------------RTLQGD 698
            |      .||.:.. ||...:::..| |..:..|..|: ||                  |...||
Human   293 S------SSLSSLR-ELYKDANLGSLSPSGIRLSDEYM-GTHASLSNRVSFASDIDVPSRVAHGD 349

  Fly   699 PLVTVDAACVASAGINCYEMATLLLYYS--TIPERSSCPFTTPATPGTTATINGHKS-------- 753
            |                       :.||  |:|.|....|..     .||..:..|:
Human   350 P-----------------------IQYSSFTLPCRKPKAFVE-----DTAKKDSLKARMRRISDW 386

  Fly   754 TEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQIGNCEILAVCPDSS 818
            |....:::::.|:.:.:.....|.     .:|..|                  |.::......|:
Human   387 TGSLSRKKRKLQEPRSKEGSDYFD-----SRSDGL------------------NTDVQGSSQASA 428

  Fly   819 LLQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVK-FI--------SVDQVTTSVAPSQLPA 874
            .  |.:|...|.|.|:.:    |.|...|.|...::.: |:        :.:.:.||...::..:
Human   429 F--LWSGGSTQILSQRSE----STHAIGSDPLRQNIYENFMRELEMSRTNTENIETSTETAESSS 487

  Fly   875 GLLPSVPALSTL----PGI----------------SQRHHDAT---KWREFFAQLEAFQRQCS-- 914
            ..|.|:..|..|    .|:                .:|..:..   ||::::..|:.    |:
Human   488 ESLSSLEQLDLLFEKEQGVVRKAGWLFFKPLVTVQKERKLELVARRKWKQYWVTLKG----CTLL 548

  Fly   915 ---TAGGRLVSALSEIRAA---------DLQGLPTRRQLYGQHRALSRALMDSQLHSLRKMGAGQ 967
               |.|...:...|..|.|         .:...|.:..::    .||.:..|..|.        |
Human   549 FYETYGKNSMDQSSAPRCALFAEDSIVQSVPEHPKKENVF----CLSNSFGDVYLF--------Q 601

  Fly   968 LARLQDLARAITSAPGSPRMTSQPQSSSSSSSGAFLNVDAAC---------KLRKVTLLFNEVDR 1023
            .....||...:|:                        |.:||         |...:.||.|:...
Human   602 ATSQTDLENWVTA------------------------VHSACASLFAKKHGKEDTLRLLKNQTKN 642

  Fly  1024 AAQRLEQLTEQRRERLRQLT------RQRALEDEISEVTSWMGSDGAENLQKFSQLHWD------ 1076
            ..|:::..::.::....||:      .::|:|::|.:   |     .:||:||   |.|
Human   643 LLQKIDMDSKMKKMAELQLSVVSDPKNRKAIENQIQQ---W-----EQNLEKF---HMDLFRMRC 696

  Fly  1077 NESQLRTQEQEFEKYYFIAMKH---LAKGR-------DLHA-------AALKVSVLS--ESANNL 1122
            ..:.|:..|....|....|...   ||.||       ..||       :||:...||  :...|.
Human   697 YLASLQGGELPNPKSLLAAASRPSKLALGRLGILSVSSFHALVCSRDDSALRKRTLSLTQRGRNK 761

  Fly  1123 K------SALDQFAQKLELAHERYDAAGRLLHL----LTQHQRENAAR----------------- 1160
            |      ..||..|:|.:...........|||:    :....|:||..
Human   762 KGIFSSLKGLDTLARKGKEKRPSITQVDELLHIYGSTVDGVPRDNAWEIQTYVHFQDNHGVTVGI 826

  Fly  1161 ------EELQRLAEQLGATSLLEKHWPV-MRKTHTLPVASSTPAA-------------------- 1198
                  |::..||.::  ..|...|:.: :||.....|....||.
Human   827 KPEHRVEDILTLACKM--RQLEPSHYGLQLRKLVDDNVEYCIPAPYEYMQQQVYDEIEVFPLNVY 889

  Fly  1199 ---------------AAGKRVSYRCSSASR----------SSFEGGPSSSNCSCWRESRNLEDMD 1238
                           |...:|..| ...||          .::..|....|.........:.|:|
Human   890 DVQLTKTGSVCDFGFAVTAQVDER-QHLSRIFISDVLPDGLAYGEGLRKGNEIMTLNGEAVSDLD 953

  Fly  1239 --QMEEEEEEQ----------------------DHDCDGGDGEE-----QQSKIADSGLGVCDNC 1274
              |||....|:                      |.|....|.:.     .||::.:..|   ||.
Human   954 LKQMEALFSEKSVGLTLIARPPDTKATLCTSWSDSDLFSRDQKSLLPPPNQSQLLEEFL---DNF 1015

  Fly  1275 ERNPK------------LARICSCQSLNEKSHDELLEDECFDRPSKRYMDIMHSPMEANAHLQCH 1327
            ::|..            |.|..:..:|::.||.|.:| :.| |.:::...:..|..::.|
Human  1016 KKNTANDFSNVPDITTGLKRSQTDGTLDQVSHREKME-QTF-RSAEQITALCRSFNDSQA----- 1073

  Fly  1328 GSSLELPKLEELSCLDP-------------KIQKTLLLIMREMIGTERDYVRSLYYVIENYIDEL 1379
             :.:|.|:..:    ||             :::|    :::|::.||:.||:.|..:.|.|::.|
Human  1074 -NGMEGPRENQ----DPPPRSLARHLSDADRLRK----VIQELVDTEKSYVKDLSCLFELYLEPL 1129

  Fly  1380 LREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELE----------------RYERNPLKVGAAF 1428
            ..|..  ..:.:...:||::.::.||... ||..||                ::.:....:|.:|
Human  1130 QNETF--LTQDEMESLFGSLPEMLEFQKV-FLETLEDGISASSDFNTLETPSQFRKLLFSLGGSF 1191

  Fly  1429 LEMESKFYLYALYNKNKPKSDTLLSEYGS-----SFFKPKQMQLQDKLDLASYLLKPVQRMGKYA 1488
            |.....|.||:.:..|..|...:|....:     :|...:....|....|.|||:|||||:.||.
Human  1192 LYYADHFKLYSGFCANHIKVQKVLERAKTDKAFKAFLDARNPTKQHSSTLESYLIKPVQRVLKYP 1256

  Fly  1489 LLLQQLV--------------KACKGVEGAALQEIAADVEELQRAEEMVKFQLRHG---NDLLAM 1536
            |||::||              :|.|     |::::|:.:.|:|:..|      .:|   :.|:|.
Human  1257 LLLKELVSLTDQESEEHYHLTEALK-----AMEKVASHINEMQKIYE------DYGTVFDQLVAE 1310

  Fly  1537 DSLRDCDVNVKEQGRLLRQ------NEFLVWQGRGGKKTLRQVFLFEELVLF------------- 1582
            .|..:.:|.....|.||..      |.||. .|:..|.....||:|:..|:.
Human  1311 QSGTEKEVTELSMGELLMHSTVSWLNPFLS-LGKARKDLELTVFVFKRAVILVYKENCKLKKKLP 1374

  Fly  1583 SKARRFPDHKNLDIYIYK-------NSIKTSDIGLTAHTGDSA------TKFEIWFRKRKPDDTW 1634
            |.:|  |.|.:.|:..:|       ::::.. :|..|.|.:::      ||.||   :.:|:..:
Human  1375 SNSR--PAHNSTDLDPFKFRWLIPISALQVR-LGNPAGTENNSIWELIHTKSEI---EGRPETIF 1433

  Fly  1635 MLQCMSEDIKNAWTEEISKLLWKQAKRNREVRLAEMSSMGIGSKPCLD-IRPSNNQI-------S 1691
            .|.|...:.|....:.|..:|.:..:|:.:..|..       .|.|.| :.|..|::       |
Human  1434 QLCCSDSESKTNIVKVIRSILRENFRRHIKCELPL-------EKTCKDRLVPLKNRVPVSAKLAS 1491

  Fly  1692 DRSIPLAQLNKTPKLRHSEPGKGSMRRPNSLISESSLSSGTSTTSGSSISGGSSSGHNDGGGRHS 1756
            .||:.:.: |.:......|.|||::...:    |.||||||. :||...:.|.....:...|::.
Human  1492 SRSLKVLK-NSSSNEWTGETGKGTLLDSD----EGSLSSGTQ-SSGCPTAEGRQDSKSTSPGKYP 1550

  Fly  1757 LHFGKLPGA----------------HSASSTNCSATLELINETQALKLAKSPSGGGIKGADTAAD 1805
             |.|....|                |..:....|.|.::..:.|.|::::.|.    ...:....
Human  1551 -HPGLADFADNLIKESDILSDEDDDHRQTVKQGSPTKDIEIQFQRLRISEDPD----VHPEAEQQ 1610

  Fly  1806 GGNTSGNGNGNG--------------NAFRSSKRNHKRSTTIVSQLSMES 1841
            .|..||.|...|              ....|:||:  |.|.:.:|:..:|
Human  1611 PGPESGEGQKGGEQPKLVRGHFCPIKRKANSTKRD--RGTLLKAQIRHQS 1658

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	PX_domain	15..>52	CDD:295365
		RhoGEF	1353..1532	CDD:279015	53/216 (25%)
		PH_puratrophin-1	1524..1663	CDD:270062	37/173 (21%)
		PH	1562..1655	CDD:278594	24/118 (20%)
TIAM2	NP_001371475.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..21
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	201..250		15/63 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	265..293		8/27 (30%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	389..417		4/50 (8%)
		PH1_Tiam1_2	505..631	CDD:269937	24/165 (15%)
		Tiam_CC_Ex	645..742	CDD:408184	23/107 (21%)
		Ubiquitin_like_fold	811..881	CDD:421700	10/71 (14%)
		PDZ_signaling	888..973	CDD:238492	13/85 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1070..1092		5/31 (16%)
		RhoGEF	1103..1292	CDD:214619	49/196 (25%)
		PH2_Tiam1_2	1294..1466	CDD:269957	39/184 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1500..1556		18/61 (30%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1568..1628		11/63 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910