Protein Alignment Pura and PLEKHG4

DIOPT Version :10

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: NP_001123199.1
• Gene: PLEKHG4 / 25894
• HGNCID: 24501
• Length: 1191
• Species: Homo sapiens
• Alignment Length: 1343
• Identity: 399/1343 - (29%)
• Similarity: 564/1343 - (41%)
• Gaps: 347/1343 - (25%)

  Fly   581 SGNSNVSDRSSSS--SSSSSSSAGEELL---TSHSEAKMRSNSVGNEASGASTSPALPLYCLGNS 640
            ||::......|||  |.....|..|.||   :||...          |.|.|.:|.:.|  :|:.
Human    79 SGDAQRGTVESSSVLSEGPGPSGVESLLCPMSSHLSL----------AQGESDTPGVGL--VGDP 131

  Fly   641 FPH------IDSDEEASDSIGPGKHHSLETCETELLPSSSIAELPE------------KLLTSGV 687
            .|.      :......||.:|.|...|..:...|..||.|  .||:            :||.||:
Human   132 GPSRAMPSGLSPGALDSDPVGLGDPLSEISKLLEAAPSGS--GLPKPADCLLAQDLCWELLASGM 194

  Fly   688 -YLPGTRTLQGDPLVTVDAACVASAGINC--YEMATLLLYYSTIPERSSCPFTTPATPGTTATIN 749
             .|||||.:||..::.:.|...|.....|  .|:..||||..:||
Human   195 ATLPGTRDVQGRAVLLLCAHSPAWLQSECSSQELIRLLLYLRSIP-------------------- 239

  Fly   750 GHKSTEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQIGNCEILAVC 814
                                :|..|...:.:.::..        ||                  .
Human   240 --------------------RPEVQALGLTVLVDAR--------IC------------------A 258

  Fly   815 PDSSLLQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVKFISVDQVTTSVAPSQLPA-GLLP 878
            |.|||.        ..|.|.|:       ..|.:...|.||....:.:|.:.:...|||: .||.
Human   259 PSSSLF--------SGLSQLQE-------AAPGAVYQVLLVGSTLLKEVPSGLQLEQLPSQSLLT 308

  Fly   879 SVPAL---STLPGISQRHHDATKWREFFAQLEAFQRQCSTAGGRLVSALSEIRAADLQGLPTR-R 939
            .:|..   ::|.|.....|.|  |.:|..:|||..:.|..|...|..|:..::|......|.. .
Human   309 HIPTAGLPTSLGGGLPYCHQA--WLDFRRRLEALLQNCQAACALLQGAIESVKAVPQPMEPGEVG 371

  Fly   940 QLYGQHRALSRALMDSQ-LHSLRKMGAGQLARLQDLARAITSAPGSPRMTSQPQSSSSSSSGAFL 1003
            ||..|...|.:.::||. |..|:..|..:|..|:.....:|.:|
Human   372 QLLQQTEVLMQQVLDSPWLAWLQCQGGRELTWLKQEVPEVTLSP--------------------- 415

  Fly  1004 NVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRAL---EDEISEVTSWMGSDGAE 1065
              |....:.|...|::.||..   |.|||.|..:|::.|...:.|   |..:.::..|:...|..
Human   416 --DYRTAMDKADELYDRVDGL---LHQLTLQSNQRIQALELVQTLEARESGLHQIEVWLQQVGWP 475

  Fly  1066 NLQKFSQLHWDNESQLRTQEQEFEKYYFIAMKHLAKGRDL-------HAAALKVSVLSESANNLK 1123
            .|::..:...|   .|...:..|::.|.:|.:.:.:|...       .||.|........|  |:
Human   476 ALEEAGEPSLD---MLLQAQGSFQELYQVAQEQVRQGEKFLQPLTGWEAAELDPPGARFLA--LR 535

  Fly  1124 SALDQFAQKLELAHERYDAAGRLLHLLTQHQRENAA-REELQRLAEQLG------ATSLLEKHWP 1181
            :.|.:|::.|....:|...|.||..|.    ||... .||.||:..:|.      ....|:.||
Human   536 AQLTEFSRALAQRCQRLADAERLFQLF----REALTWAEEGQRVLAELEQERPGVVLQQLQLHW- 595

  Fly  1182 VMRKTHTLPVASSTPAAAAGKRVSYRCSSASRSSFEGGPSSSNCSCWRESRNLEDMDQMEEEEEE 1246
              .:...||.|            .:|                  ..|..:..|
Human   596 --TRHPDLPPA------------HFR------------------KMWALATGL------------ 616

  Fly  1247 QDHDCDGGDGEEQQSKIA-----DSGLGVCDNCERNPKL------ARICSCQSLNEKSHDELLED 1300
                  |.:...|:.:.|     |:.|.:....|.:.||      |.:|..|.....:|..|.:.
Human   617 ------GSEAIRQECRWAWARCQDTWLALDQKLEASLKLPPVGSTASLCVSQVPAAPAHPPLRKA 675

  Fly  1301 ECFDRPSKRYMDIMHSPMEANAHLQCH--------------GSSL--ELPKLEELSCLDPKIQKT 1349
            ..|||      ::..|..|...|  ||              |.:|  ..|.:......||:....
Human   676 YSFDR------NLGQSLSEPACH--CHHAATIAACRRPEAGGGALPQASPTVPPPGSSDPRSLNR 732

  Fly  1350 LLLIMREMIGTERDYVRSLYYVIENYIDELLREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGEL 1414
            |.|::.||:.|||:|||:|.|.:|||..||.|.|:||.|||||..:|||:||:.:||...||.||
Human   733 LQLVLAEMVATEREYVRALEYTMENYFPELDRPDVPQGLRGQRAHLFGNLEKLRDFHCHFFLREL 797

  Fly  1415 ERYERNPLKVGAAFLEMESKFYLYALYNKNKPKSDTLLSEYGSSFFKPKQMQLQDKLDLASYLLK 1479
            |...|:|.:|..|||....:|.:||||:||||:||.|:|.||.:|||.||..|.|.|||||||||
Human   798 EACTRHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMSSYGHTFFKDKQQALGDHLDLASYLLK 862

  Fly  1480 PVQRMGKYALLLQQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDV 1544
            |:|||||||||||:|.:||    |...||::|    |:.|:.:|.|||||||||||||:::.|||
Human   863 PIQRMGKYALLLQELARAC----GGPTQELSA----LREAQSLVHFQLRHGNDLLAMDAIQGCDV 919

  Fly  1545 NVKEQGRLLRQNEFLVWQGRGGKKTLRQVFLFEELVLFSKARRFPDHKNLDIYIYKNSIKTSDIG 1609
            |:||||:|:||:||:|..||  .|::|::||||||:||||.|..|  ..:|.:.||.|.|.:|:|
Human   920 NLKEQGQLVRQDEFVVRTGR--HKSVRRIFLFEELLLFSKPRHGP--TGVDTFAYKRSFKMADLG 980

  Fly  1610 LTAHTGDSATKFEIWFRKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREVRLAEMSSMG 1674
            ||...|:|..:||||||:||..||::||..|..||.|||.:||.|||:||..|:|||:|||.|||
Human   981 LTECCGNSNLRFEIWFRRRKARDTFVLQASSLAIKQAWTADISHLLWRQAVHNKEVRMAEMVSMG 1045

  Fly  1675 IGSKPCLDIRPSNNQISDRSIPLAQLNKTPKLRHSEPGKGSMRRPNSLISESSLSSGTSTTSGSS 1739
            :|:|...||.||...|:||::     |...|.|.               ..|..|...:.....|
Human  1046 VGNKAFRDIAPSEEAINDRTV-----NYVLKCRE---------------VRSRASIAVAPFDHDS 1090

  Fly  1740 ISGGSSSGHNDGGGRHSLHFGKLPGAHSASSTNCSATLELINETQALKL---------------- 1788
            :..|:|:              .|||..::.|...|..|.|..:...|.|
Human  1091 LYLGASN--------------SLPGDPASCSVLGSLNLHLYRDPALLGLRCPLYPSFPEEAALEA 1141

  Fly  1789 ------------------AKSPSGGGIKGADTAADGGNTSGNG 1813
                              ::.||..|..|:|::...|...|.|
Human  1142 EAELGGQPSLTAEDSEISSQCPSASGSSGSDSSCVSGQALGRG 1184

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	39/157 (25%)
		RhoGEF	1353..1524	CDD:459876	94/170 (55%)
		PH_puratrophin-1	1524..1663	CDD:270062	79/138 (57%)
PLEKHG4	NP_001123199.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..152		21/84 (25%)
		SEC14	193..321	CDD:469559	41/208 (20%)
		SPEC	447..>598	CDD:413338	35/162 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	707..728		4/20 (20%)
		RhoGEF	736..898	CDD:459876	94/169 (56%)
		PH_puratrophin-1	899..1034	CDD:270062	79/138 (57%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1150..1176		5/25 (20%)