DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pura and PLEKHG4B

DIOPT Version :9

Sequence 1:	NP_996016.2	Gene:	Pura / 2768948	FlyBaseID:	FBgn0035802	Length:	1904	Species:	Drosophila melanogaster
Sequence 2:	NP_443141.4	Gene:	PLEKHG4B / 153478	HGNCID:	29399	Length:	1627	Species:	Homo sapiens

Alignment Length:	1839	Identity:	475/1839 - (25%)
Similarity:	699/1839 - (38%)	Gaps:	604/1839 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   319 VDATLARLIDSHLSHIYPVYWARTRAILIQ--QARERLVC-GFDG-----SLVRFEQRFLCKFAQ 375
            :||.:.|.    ||.:||.:.|....:|.|  ...||  | |.||     |.:...:|.|....|
Human    20 LDACIQRT----LSALYPPFEATAATVLWQLFSVAER--CHGGDGLHCLTSFLLPAKRALQHLQQ 78

  Fly   376 IAATLRTAHQIGESWLWHPGWPLATSNGALVLQLSDAEIAHALR--PSELYLCIKLESTESD--- 435
            .|.    |...|..:| ||||||. ::..:|:||:.   .|.:|  |.:.||.:.....:|.
Human    79 EAC----ARYRGLVFL-HPGWPLC-AHEKVVVQLAS---LHGVRLQPGDFYLQVTSAGKQSARLV 134

  Fly   436 ADCKPRL---------------------YAVWRSSRKEESVQSHCI------------------- 460
            ..|..||                     :..|.:..:.......|:
Human   135 LKCLSRLGRGTEEVTVPEAMYGCVFTGAFLEWVNRERRHVPLQTCLLTSGLAVHRAPWSDVTDPV 199

  Fly   461 -------------DHENPGELPLSVLQLEMSTAAAA------------LMASDC----------- 489
                         ....|..||.|..:..:.|.|.|            .:.|.|
Human   200 FVPSPGAILQSYSSCTGPERLPSSPSEAPVPTQATAGPHFQGSASCPDTLTSPCRRGHTGSDQLR 264

  Fly   490 -------GEL--PQLLQNLLVSVERSIERVSLNEL--QMPMHHDRGE----DSLDEANNNGCNNN 539
                   .||  |:.|..   |.:|..|:||.:|.  :||..:..|.    |.:|:.:.
Human   265 HLPYPERAELGSPRTLSG---SSDRDFEKVSPSEQGPRMPPENCGGSGERPDPMDQEDR------ 320

  Fly   540 LLGSPKCALRRSDL---------------ITKNKLFNSRYM--LRRLTNRQDSMNSTSSGNSN-- 585
                ||.....:||               :...:.|...||  ||         |....|:|.
Human   321 ----PKALTFHTDLGIPSSRRRPPGDPTCVQPRRWFRESYMEALR---------NPMPLGSSEEA 372

  Fly   586 VSDRSSSSSSSSSSSAGEELLTSHSEAKMRSNSVGNEASGASTSPALPLYCLGNSFPHIDSDEEA 650
            :.|.:.||.:.:|...|...:.|.::         .|.||....|        ...|.::.:...
Human   373 LGDLACSSLTGASRDLGTGAVASGTQ---------EETSGPRGDP--------QQTPSLEKERHT 420

  Fly   651 SDSIGPG------------------------------KHHSLE---------------------- 663
            ....|||                              .|||..
Human   421 PSRTGPGAAGRTLPRRSRSWERAPRSSRGAQAAACHTSHHSAGSRPGGHLGGQAVGTPNCVPVEG 485

  Fly   664 ---TCETELLPSSS----------------------------------------------IAELP 679
               |.|.::|.||:                                              :.::.
Human   486 PGCTKEEDVLASSACVSTDGGSLHCHNPSGPSDVPARQPHPEQEGWPPGTGDFPSQVPKQVLDVS 550

  Fly   680 EKLLTSGVY-LPGTRTLQGDPLVTVDAACV--ASAGINCYEMATLLLYYSTIPERSSCPF----- 736
            ::||.|||. |||||...|..:|.|....:  .....:|.|:..||||:.:||.:.....
Human   551 QELLQSGVVTLPGTRDRHGRAVVQVRTRSLLWTREHSSCAELTRLLLYFHSIPRKEVRDLGLVVL 615

  Fly   737 ----TTPATPGTTATINGHKSTEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQS 797
                .:||.|..:..::|              .|....|.  ..:||:.::|..
Human   616 VDARRSPAAPAVSQALSG--------------LQNNTSPI--IHSILLLVDKES----------- 653

  Fly   798 LRLLAKQIGNCEILAVCPDSSLLQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVKFISVDQ 862
                          |..||...:..|                             ::|.  |:..
Human   654 --------------AFRPDKDAIIQC-----------------------------EVVS--SLKA 673

  Fly   863 VTTSVAPSQLPAGLLPSVPALSTLPGISQRHHDATKWREFFAQLEAFQRQCSTAGGRLVSALSEI 927
            |...|...||.|.|..|.|         ..|.|   |..|..:||.|...|..|   ::...:..
Human   674 VHKFVDSCQLTADLDGSFP---------YSHGD---WICFRQRLEHFAANCEEA---IIFLQNSF 723

  Fly   928 RAADLQGLPTRRQ----LYGQHRALSR-ALMDSQLHSLRKMGAGQLARL--QDLARAITSAPGSP 985
            .:.:....|...|    |..||..:.: .|.|..|.|||..|...||||  ::|.
Human   724 CSLNTHRTPRTAQEVAELIDQHETMMKLVLEDPLLVSLRLEGGTVLARLRREELG---------- 778

  Fly   986 RMTSQPQSSSSSSSGAFLNVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALED 1050
                              ..|:...|...|.|::.||....|| .||.  ..||:||       :
Human   779 ------------------TEDSRDTLEAATSLYDRVDEEVHRL-VLTS--NNRLQQL-------E 815

  Fly  1051 EISEVTSWMGSDGAENLQKFSQLHWDNESQLRTQEQEFEKYYFIAMKHLAKGRDLHAAA-LKVSV 1114
            .:.|:.|.:..:..::.||..||..:|..:.....|:|.: ...|:...|:.|:...|. .:.|.
Human   816 HLRELASLLEGNDQQSCQKGLQLAKENPQRTEEMVQDFRR-GLSAVVSQAECREGELARWTRSSE 879

  Fly  1115 LSESANNLKSALDQFA---------------QKLELAHERYDAAGRLLHLLTQHQ--RENAAREE 1162
            |.|:.::....||..|               :...:|.|.:..||  :.:|..|.  :..|::::
Human   880 LCETVSSWMGPLDPEACPSSPVAECLRSCHQEATSVAAEAFPGAG--VAVLKPHALGKPWASQQD 942

  Fly  1163 L--------QRLAEQLGATS-------------LLEKHWPVMRKTHTLPVASSTPAAAAGKRVSY 1206
            |        .||.|.|...:             ..|:....||:....|...||.:.......:.
Human   943 LWLQYPQTRLRLEEALSEAAPDPSLPPLAQSPPKHERAQEAMRRHQKPPSFPSTDSGGGAWEPAQ 1007

  Fly  1207 RCSSASRSSFEGGPSSSN-----CSCW-------------RESRNLEDMDQMEEEEEEQDHDCDG 1253
            ..|.....:...|.....     |:.|             ..:.:|||......|..:.
Human  1008 PLSGLPGRALLCGQDGETLRPGLCALWDPLSLLRGLPGAGATTAHLEDSSACSSEPTQT------ 1066

  Fly  1254 GDGEEQQSKIADSGLGVCDNCERNPKLARICSCQSLNEKSHDELLEDECFDRPSKRYMDIMHSPM 1318
                            :.....::|:...|...||......|....|.|  :|.  :..:....:
Human  1067 ----------------LASRPRKHPQKKMIKKTQSFEIPQPDSGPRDSC--QPD--HTSVFSKGL 1111

  Fly  1319 EANA--------HLQCHGSSLELPKLEELSC---LDPKIQ--------KTLLLIMREMIGTERDY 1364
            |..:        .|..|..|..:.:...||.   |.|..:        ..|..||.|||.|||:|
Human  1112 EVTSTVATEKKLPLWQHARSPPVTQSRSLSSPSGLHPAEEDGRQQVGSSRLRHIMAEMIATEREY 1176

  Fly  1365 VRSLYYVIENYIDELLREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELERYERNPLKVGAAFL 1429
            :|.|.|||:||..|:.|.|:||.|||:.:|||||:||:.:||..|||.||||.:..||.||.:||
Human  1177 IRCLGYVIDNYFPEMERMDLPQGLRGKHHVIFGNLEKLHDFHQQHFLRELERCQHCPLAVGRSFL 1241

  Fly  1430 EMESKFYLYALYNKNKPKSDTLLSEYGSSFFKPKQMQLQDKLDLASYLLKPVQRMGKYALLLQQL 1494
            ..|.:|.:|.:|:||||:||.|||.:|::|||.||.:|.||:|||||||:||||:.|||||||.|
Human  1242 RHEEQFGMYVIYSKNKPQSDALLSSHGNAFFKDKQRELGDKMDLASYLLRPVQRVAKYALLLQDL 1306

  Fly  1495 VK--ACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNVKEQGRLLRQNE 1557
            :|  :|...:|   ||:.    ||:.||.:|.|||||||||||||::|.||||:||||:|..::|
Human  1307 LKEASCGLAQG---QELG----ELRAAEVVVCFQLRHGNDLLAMDAIRGCDVNLKEQGQLRCRDE 1364

  Fly  1558 FLVWQGRGGKKTLRQVFLFEELVLFSKARRFP-DHKNLDIYIYKNSIKTSDIGLTAHTGDSATKF 1621
            |:|..||  ||.||.|||||:|:||||.::.. .|   |:|:||.|.||::||:|.:.|||..:|
Human  1365 FIVCCGR--KKYLRHVFLFEDLILFSKTQKVEGSH---DVYLYKQSFKTAEIGMTENVGDSGLRF 1424

  Fly  1622 EIWF-RKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREVRLAEMSSMGIGSKPCLDIRP 1685
            |||| |:||..||::||..|.::|:|||:.|.::||:||.::||:|:.||:|||||::|.:|::|
Human  1425 EIWFRRRRKSQDTYILQASSAEVKSAWTDVIGRILWRQALKSRELRIQEMASMGIGNQPFMDVKP 1489

  Fly  1686 SNNQ-----ISDRSIPLAQL-NKTP---------KLR---------HSEPGKGSMRRPNSLISES 1726
            .:..     ||||:...|.: ::.|         ::|         |:.|    .:||:|.||:|
Human  1490 RDRTPDCAVISDRAPKCAVMSDRVPDSIVKGTESQMRGSTAVSSSDHAAP----FKRPHSTISDS 1550

  Fly  1727 SLSSGTSTTSGSSISG------GSSSGHNDGGGRHSLHFGKLPG----AHSASSTNCSATL---- 1777
            |.||  |::..|||.|      .||..|              ||    |||..|::..|.:
Human  1551 STSS--SSSQSSSILGSLGLLVSSSPAH--------------PGLWSPAHSPWSSDIRACVEEDE 1599

  Fly  1778 ---ELINETQALKLAKSPS 1793
               ||...|||.....:|:
Human  1600 PEPELETGTQAAVCEGAPA 1618

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	PX_domain	15..>52	CDD:295365
		RhoGEF	1353..1532	CDD:279015	103/180 (57%)
		PH_puratrophin-1	1524..1663	CDD:270062	78/140 (56%)
		PH	1562..1655	CDD:278594	48/94 (51%)
PLEKHG4B	NP_443141.4	Herpes_ICP4_C	186..>468	CDD:332854	53/320 (17%)
		RhoGEF	1165..1308	CDD:306973	87/142 (61%)
		PH_puratrophin-1	1332..1467	CDD:270062	78/139 (56%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	207	1.000	Domainoid score	I2897
eggNOG	1	0.900	-	-		E1_KOG0689
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	413	1.000	Inparanoid score	I1855
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D263447at33208
OrthoFinder	1	1.000	-	-		FOG0005689
OrthoInspector	1	1.000	-	-		otm41872
orthoMCL	1	0.900	-	-		OOG6_106140
Panther	1	1.100	-	-	O	PTHR45845
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R5143
SonicParanoid	1	1.000	-	-		X1908
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	12	11.860