DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pura and Mcf2l

DIOPT Version :9

Sequence 1:	NP_996016.2	Gene:	Pura / 2768948	FlyBaseID:	FBgn0035802	Length:	1904	Species:	Drosophila melanogaster
Sequence 2:	XP_006253478.1	Gene:	Mcf2l / 117020	RGDID:	619782	Length:	1280	Species:	Rattus norvegicus

Alignment Length:	1387	Identity:	301/1387 - (21%)
Similarity:	508/1387 - (36%)	Gaps:	372/1387 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly   648 EEASDSIGPGKHHSLETCETELLPSSSIAELPEKLLTSGVYLPGTRTLQGDPLVTVDAACVASAG 712
            ||....:.....::.|....:::|..: |::.|:|.....||.|.|...|.|::|. ....|.:.
  Rat   112 EEMVQRLNAVSKNTDEIMHQDIVPLCA-ADIQEQLKKRFAYLSGGRGQDGSPVITF-PDYPAFSE 174

  Fly   713 INCYEMATLLLYYSTIP-------------ERSSCPFTT------------PATPGTTATIN--- 749
            |...|...::.|.::||             :|....:|:            ||.......:.
  Rat   175 IPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTG 239

  Fly   750 ---------GHKSTEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQ------------H---LC- 789
                     ..|....:.:.:.........|....:     |:|||            |   ||
  Rat   240 FFQRTLSDIAFKFNRDEFKMKVPVMMLSSVPELHGY-----IDKSQLTEDLGGTLDYCHSRWLCH 299

  Fly   790 ---------VIDLICQSLRLLAKQIGNCEILAVCPDSSLLQLCNGQQQQKLQQQQQ---QNNNSD 842
                     ::....|.|:....::...|:......:||:...:.:::.|:::..|   ...||.
  Rat   300 RTAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALTEGNSI 364

  Fly   843 HPEPSSPSPVDLVKFISVDQVTTSVAPSQLPAGLLPSVPALSTLPGISQRHHDATKWREFFAQLE 907
            ......|....:|..::.||:.......:|                ::|.:.....:.||:|:.:
  Rat   365 LESLREPLAESIVHSVNQDQLDNQATVKRL----------------LTQLNETEAAFDEFWAKHQ 413

  Fly   908 AFQRQCSTAGGRLVSALSEIRAADLQGLPTRRQLYGQHRALSRAL--MDSQLHSLRKMGAGQLAR 970
            ....||           .::|..: ||.          |.:..||  |..::.:...:| ..||.
  Rat   414 QKLEQC-----------LQLRHFE-QGF----------REVKTALDSMSQKIAAFTDVG-NSLAH 455

  Fly   971 LQDLARAITSAPGSPRMTSQPQSSSSSSSGAFLNVDAACKLRKVTLLFNE---VDRAAQRLEQLT 1032
            :|.|.:.:|        |.:.:||.:......|:::..      .|:.|.   ||....:.|:|.
  Rat   456 VQHLLKDLT--------TFEEKSSVAVDKARALSLEGQ------QLIENRHYAVDSIHPKCEELQ 506

  Fly  1033 EQRRERLRQLTRQRALEDEISEVTSWMGSDGAENLQKFSQLHWDNESQLRTQEQEFEKYYFIAMK 1097
            ........::||:|.|..:..|:.|.:.          :.:.|.:|.......|..:|
  Rat   507 HLCDHFASEVTRRRDLLSKSLELHSLLE----------TSMKWSDEGIFLLASQPVDK------- 554

  Fly  1098 HLAKGRDLHAAALKVSVLSESANNLKSALDQFAQKLELAHERYDA---AGRLLHLLTQHQRENAA 1159
              .:.:|...|||:     |....|::..:...|:|...::.|:.   ...|.|:....|::.:.
  Rat   555 --CQSQDGAEAALQ-----EIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQEST 612

  Fly  1160 REELQRLAEQLGATSLLEKHWPVMRKTHTLPVA-SSTPAAAAGKRVSYRCSSASRSSFEGGPSSS 1223
            .|...|  .|.....|..|....::.....|.| :.:|:.:.|...|...||:..::...||
  Rat   613 EEMFHR--RQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNALRRGP--- 672

  Fly  1224 NCSCWRESRNLEDMDQMEEEEEEQDHDCDGGDGEEQQSKIADSGLGVCDNCERNPKLARICSCQS 1288
                :|.:::     :|.|..:.:    ....|||::|.
  Rat   673 ----YRRAKS-----EMSEPRQGR----TSSTGEEEESL-------------------------- 698

  Fly  1289 LNEKSHDELLEDECFDRPSKRYMDIMHSPMEANAHLQCHGSSLELPKLEELSCLDPKIQKTLLLI 1353
                                             |.|:.|                         :
  Rat   699 ---------------------------------AILRRH-------------------------V 705

  Fly  1354 MREMIGTERDYVRSLYYVIENYIDE----LLREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGEL 1414
            |.|::.|||.||..|..|:|.|..|    |:...|...|:.::|::|||:|:|:.|||..||.||
  Rat   706 MNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLREL 770

  Fly  1415 ERYERNPLKVGAAFLEMESKFYLYALYNKNKPKSDTLLSEYGS-SFFKPKQMQLQDKLDLASYLL 1478
            |.....|..||..|||...:|.:|..|.:|||:|::|..:... .||:..|.:|..||.|.||||
  Rat   771 ESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLL 835

  Fly  1479 KPVQRMGKYALLLQQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCD 1543
            |||||:.||.|||::::|..|..|||         |:||.|...:...|:..||.:.:.::...|
  Rat   836 KPVQRITKYQLLLKEMLKYSKHCEGA---------EDLQEALSSILGILKAVNDSMHLIAITGYD 891

  Fly  1544 VNVKEQGRLLRQNEFLVW----QGRGGKKTL-------RQVFLFEELVLFSKAR--RFPDHKNLD 1595
            .|:.:.|:||.|..|.||    :|....|.|       |.:||.|:.|||.|.|  ....::...
  Rat   892 GNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAP 956

  Fly  1596 IYIYKNSIKTSDIGLTAHTGDSATKFEIWFRKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAK 1660
            .|.||.|:..:.:|:|.:......|||||:..|  ::.:::|..:.:||.||..||.|:|..|.:
  Rat   957 SYSYKQSLNMTAVGITENVKGDTKKFEIWYNAR--EEVYIIQAPTPEIKAAWVNEIRKVLTSQLQ 1019

  Fly  1661 RNREV---RLAEMS-SMGI----GSKPCLDIRPSNNQISDRSI-PL-------AQLNKTPKLRHS 1709
            ..||.   |..|.| |:.:    .:.|......:..::.||.. ||       :.|.|.|
  Rat  1020 ACREASQHRALEQSHSLPLPTPASTSPTKGSTRNVKKLEDRKTDPLCLEGCVSSSLPKPP----- 1079

  Fly  1710 EPGKGSMRRPNSLISESSLSSGTSTTSG-SSISG--GSSSGHNDGGGRHSLHFGKLP-GAHSASS 1770
            |.||.. ..|:|....|:||....|..| :::.|  .|.:..:....||.:.....| |....|.
  Rat  1080 EKGKDD-AVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSK 1143

  Fly  1771 TN-----------CSATLELINETQALKLAKSPSGGG----IKGADTAADGGNTSGNGNGNGNAF 1820
            |:           .|:..||||.:.    |:...|.|    :.|..|....|...|:
  Rat  1144 TSHSLEAPEEDGGWSSAEELINSSD----AEEDGGVGPRKLVPGKYTVLMDGEKGGS-------- 1196

  Fly  1821 RSSKRNHKRSTTIVSQLSMESGIVSDMCVTPDQEQSAAESSRSSW-----STSTTGASGASTTSA 1880
                          ..|:|.||         |..:...|.:...|     ::|..|...||  |.
  Rat  1197 --------------DTLAMRSG---------DMVEVVEEGTEGLWYVRDLTSSKEGWVPAS--SL 1236

  Fly  1881 STVILRRYRSYAHAAESASPES 1902
            :|::.:...:...::..:||.|
  Rat  1237 ATLLGKSSSAQCLSSSDSSPGS 1258

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	PX_domain	15..>52	CDD:295365
		RhoGEF	1353..1532	CDD:279015	74/183 (40%)
		PH_puratrophin-1	1524..1663	CDD:270062	46/151 (30%)
		PH	1562..1655	CDD:278594	32/101 (32%)
Mcf2l	XP_006253478.1	SEC14	145..290	CDD:214706	24/150 (16%)
		SPEC	421..623	CDD:238103	48/253 (19%)
		RhoGEF	702..879	CDD:238091	75/210 (36%)
		PH_Dbs	886..1017	CDD:269934	42/132 (32%)
		PH	900..1014	CDD:278594	38/115 (33%)
		SH3_DBS	1184..1236	CDD:212791	14/84 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910