Protein Alignment Pura and Mcf2

DIOPT Version :10

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: XP_006527813.1
• Gene: Mcf2 / 109904
• MGIID: 96932
• Length: 1127
• Species: Mus musculus
• Alignment Length: 1010
• Identity: 250/1010 - (24%)
• Similarity: 410/1010 - (40%)
• Gaps: 305/1010 - (30%)

  Fly   959 SLRKMGAGQLARLQDLARAITSAPGS------PRMTSQPQSSSSSSSGAFLNVDAACKLRKVTL- 1016
            ||:|:.|              |.|||      .|.||..|.:.:.....|...|...||..|.|
Mouse   195 SLQKISA--------------SFPGSLHLVLVLRPTSFLQRTFTDIGFRFSQEDFMLKLPVVMLS 245

  Fly  1017 ----LFNEVDRAAQRLEQLTEQRRERLRQ-----LTRQRALED---EISEVTSWMGSDGAENLQK 1069
                |...:|.     :|||.:....|:.     :..:.|:|.   .:.|:...:.|.|.|..:
Mouse   246 SVSDLLTYIDD-----KQLTPELGGTLQYCHSEWIIFRNAIEKFAVTVKEMAQMLQSFGTELAE- 304

  Fly  1070 FSQLHWDNESQLRTQEQEFEKYYFIAMKHLAKGRDLHAAA---------LKVSVLSES------- 1118
             ::|..|..|.........|:|      ||.| .||.|..         |:|....|:
Mouse   305 -TELPQDILSIEEILAGRAERY------HLLK-NDLTAVTKEGKVLLMNLQVPATEETVSSSLEC 361

  Fly  1119 -----------------ANNLKSALDQFAQKLELAHERY-----------DAAGRLLHLLTQHQR 1155
                             .:::::|.|.|.:|.:|..|:|           :|..::..||:| ||
Mouse   362 TQHINGDWQTIKKLLAQVHDMETAFDGFWEKHQLKMEQYLQLWKFEQDFQEAVTQVEFLLSQ-QR 425

  Fly  1156 E------NAA--REELQRLAEQLGATS--LLEKHWPVMRKTHTLPVASSTPAA------------ 1198
            |      |.|  ::.|::| |.|...|  ||.|...|:.:.|.|  ||:...|
Mouse   426 ELGDITGNLAQVKQRLKKL-EILDDKSQELLTKARIVILRGHKL--ASNHHYALDLICQRCNELR 487

  Fly  1199 ---------AAGKRVS-------YRCSSASRSSFEGGPSSSNCSCWRESRNLEDMDQMEEEEEEQ 1247
                     ...|||.       :|....:|...:.|      .|...|   :.||:.:.:|:.|
Mouse   488 YLSDILVNEIRTKRVQLSRTFKVHRLLQQARQCCDQG------ECLLAS---QGMDKFQTKEDAQ 543

  Fly  1248 -----------------DHDCDGGDGE-------EQQSKIADSGLGVCDNCERNPKLARICS--- 1285
                             ::|.:....|       |.::::.:..|          ||..|.|
Mouse   544 KALQDVDNFLQMAMPFINYDIESLQYEFDVLLSPELKAQMQNIQL----------KLENIRSAFQ 598

  Fly  1286 -----CQSLNE-----------KSHDELLEDECFDRPSKRYMDIMHSPMEA--------NAHLQC 1326
                 |:||.|           .|.:.:.....|..|  :::.|.:|..:|        .:..|.
Mouse   599 NQQAGCKSLKEVPEGAFQNLVPASENVMRSRMIFFSP--KHVGIGYSFFQACRIFLKVKKSWRQI 661

  Fly  1327 HG-SSLELPKLEEL----SCLDPKIQKTLLL----IMREMIGTERDYVRSLYYVIENYIDEL--- 1379
            |. |::::..:|:.    |...||:..:|.:    ::.|:|.|||.|||.|:.|:..|..|:
Mouse   662 HAQSNVKVEAVEDSQEKNSDQSPKLDNSLDILKNHVLNELIQTERAYVRELFTVLLGYRSEMDNP 726

  Fly  1380 -LREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELERYERNPLKVGAAFLEMESKFYLYALYNK 1443
             :.:.:|..||.:::|:|||:.:|:||||:.|:..||.....|.:||..|||.:..|.:||.|.:
Mouse   727 QMFDLMPPLLRNKKDVLFGNMAEIYEFHNNIFMSRLEDCSDAPERVGPCFLERKDDFQMYAKYCQ 791

  Fly  1444 NKPKSDTLLSEYGS-SFFKPKQMQLQDKLDLASYLLKPVQRMGKYALLLQQLVKACKGVEGAA-L 1506
            |||:|:.:..:|.. :||:..|.:|:.:|.|.|||||||||:.||.|||::|:|..|..||.. |
Mouse   792 NKPRSELIWRKYSECAFFQECQRKLKHRLGLDSYLLKPVQRITKYQLLLKELLKYSKEGEGTTKL 856

  Fly  1507 QEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNVKEQGRLLRQNEFLVWQG-RGG---- 1566
            :|....:.||          |:..||.:...::.....|:.|.|:::.|..|.||.| |.|
Mouse   857 KEALDSMLEL----------LKSVNDSMHQTAINGYVGNINELGKMVLQGSFNVWLGHRKGATKM 911

  Fly  1567 ------KKTLRQVFLFEELVLFSKARRFPDHKNLD---IYIYKNSIKTSDIGLTAHTGDSATKFE 1622
                  |...|.:||:|:.|:|.| |||...:..|   .|.:|:.:|..|:|:|.|......|||
Mouse   912 KDFARFKPMQRHLFLYEKAVMFCK-RRFESGEGADRYPSYSFKHCLKMEDVGITEHVKGDNRKFE 975

  Fly  1623 IWFRKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREVRLAEMSSMGIGSKPCLDIRPSN 1687
            |  |..:.::.:::|..:.|:|..|.:||.|:|.:|    :|:..|:..
Mouse   976 I--RYSEKEEIYIVQAPNVDVKMLWLKEIRKILVQQ----KELMTAKTQ---------------- 1018

  Fly  1688 NQISDRSIPLAQLNKTPKLRHSEPGKGSMRRPNSLISESSLSSGTSTTSGSSI------------ 1740
               .|:::...||  .|:.:::|..|.|   |..:..|:..:   :|.:|:.:
Mouse  1019 ---QDQALDQDQL--FPQQQNAELCKSS---PFCVCEETLFN---ATEAGAEVEQAGALIKVREA 1072

  Fly  1741 -----SGGSSSGHNDGGGRHSLHFGKLPGAHSASS 1770
                 :..||:..|          |.||.|..|::
Mouse  1073 AVLAQAEASSAAWN----------GMLPSAEGAAA 1097

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	42/206 (20%)
		RhoGEF	1353..1524	CDD:459876	71/176 (40%)
		PH_puratrophin-1	1524..1663	CDD:270062	46/152 (30%)
Mcf2	XP_006527813.1	SEC14	128..268	CDD:214706	23/91 (25%)
		SPEC	408..599	CDD:413338	43/213 (20%)
		RhoGEF	694..871	CDD:238091	72/186 (39%)
		PH-like	878..1009	CDD:473070	42/133 (32%)