Protein Alignment Pura and tiam2a

DIOPT Version :9

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: XP_009291563.1
• Gene: tiam2a / 100334442
• ZFINID: ZDB-GENE-030219-98
• Length: 1686
• Species: Danio rerio
• Alignment Length: 2111
• Identity: 376/2111 - (17%)
• Similarity: 652/2111 - (30%)
• Gaps: 828/2111 - (39%)

  Fly    30 RSSMSFVRRRQEAFKWIRRRLARRQCHADTNIETGRAEAAVTGSGAGAELAEILVPSDTQIDVPG 94
            :||.:|:.|:   :.:|.::|.                       :|::..|  ||.|.   :||
Zfish    67 KSSRAFISRQ---YDFITQKLK-----------------------SGSQWDE--VPEDL---LPG 100

  Fly    95 GGIPGGQIPSLESTDRD--------------------MKEARAAPIARDDEDQEAGDP----EEH 135
            |.:   .:||    ::|                    ||....:....|.|||.:  |    ::.
Zfish   101 GCL---MLPS----EKDCLYRNHKERRKTCNKYALNAMKTPERSLRLEDIEDQSS--PRVVIKKD 156

  Fly   136 GEEEDE---------------EPPEVEQLSANANYVC-CRYPGTTSSSTSGAADTLNRSKSRVQK 184
            |....|               ||.::.:.|......| ....|.:..|.:|||..::..||
Zfish   157 GSVRVEFNNSPNSALILNNCTEPVQLLKFSPTMESTCGLSAAGPSLDSHNGAAQAMSSLKS---- 217

  Fly   185 YLKKCKQRLTGQAKMPTSSSTTTTTITLIRNEPAPTTIAERESEQALAEQEEELLEVEDLTEGSN 249
                             |..::.::...:.:.|..|::...:|:.|..:|:.:.:..      :.
Zfish   218 -----------------SKGSSLSSDGSLYDLPWRTSVELNDSDNASPDQQRQTVST------NT 259

  Fly   250 GSYTDALP----------------------IECSLSASIAEEQQQGVDELEPPKEEEVPLE---T 289
            |.:.:.||                      ::|| :.|.:..:...|..:|....|.||.|   :
Zfish   260 GFFDEQLPAITTTDLYRDPRIASTFPTVKDLQCS-NGSRSHHRSSFVSVMEGLSSEGVPEEQQYS 323

  Fly   290 ELTEP--SSTPPPQSNPEGIPAPERFRLTADVDATLARLIDSHLSHIYPVYWARTRAILIQQAR- 351
            ..|.|  .|...|::|.:......|||..:|...:|                  ||....|:.|
Zfish   324 SFTLPCRKSKASPENNGKKASIRSRFRRISDWTGSL------------------TRKKKFQEPRY 370

  Fly   352 -ERLVC---GFDG-------------------------SLVR------------------FEQRF 369
             |.:.|   |.||                         ||.|                  ..|..
Zfish   371 NEPVDCFDSGVDGLTADTSSPSQVSSLMATSNGMSQSCSLSRSLHSRSGSAGALNLGNDILRQNI 435

  Fly   370 LCKFAQIAATLRTAHQIG-ESW--------------------------------LWHPGW----P 397
            ...|.....|.|...:.| |.|                                :...||    |
Zfish   436 YKNFMMELKTARLPAERGAEDWCESSSEETGSSSQEIPGSLEQLDLLFEKEQGVVRRAGWLSFKP 500

  Fly   398 LATSNGALVLQLSDAEIAHALRPSELYLCIK-----------LESTESDADCKPRLYAVWRSSRK 451
            |.|     |.:....|:....:..:.::.:|           ..|:|.::.|    ||::.....
Zfish   501 LLT-----VHKDRKLELVSRRKWKQFWVTLKGCTLLFYETYGKASSEPESPC----YALFAEDGV 556

  Fly   452 EESVQSH-------CIDHENPGELPL----SVLQLE-----MSTAAAALMASDCGELPQL----- 495
            .::|..|       |:.: ..|::.|    |...||     :.:|:|:|.|...|:...|
Zfish   557 VQAVPEHPKKENIFCLSN-TYGDVYLFQASSQTDLENWVTAIHSASASLFAKRYGKEDTLRLLRG 620

  Fly   496 -LQNLLVSVERSIERVSLNELQM-----PMHHDRGEDSLDEANNNGCNNNLLGSPKCALRRSDLI 554
             ::.||..::...:...:.|||:     |.:....|:.:.:...|             |.|.::
Zfish   621 QIRGLLQKIDMDGKMKKMAELQLSVVGDPKNRKAIENQIQQWEQN-------------LERFNM- 671

  Fly   555 TKNKLFNSRYMLRRLTNRQ----DSMNSTSSGNSNVSDRSSSSSSSSSSSAGEELLTSHSEAKMR 615
               .||..|..|..|...:    .|:.:|:|..:..:.......|.||..|   |:.|..||.||
Zfish   672 ---DLFRMRCYLASLQGGELPNPKSLLATASRRTKAALARLGIFSVSSFHA---LICSRDEATMR 730

  Fly   616 SNSVGNEASGASTSPALPLYCLGNSFPHIDSDEEASDSI---GPGKHHSLETCETELLPSSSIAE 677
            ..|             |.|...|.|...:.|..:..|::   |..|.|||....:| |...|.:.
Zfish   731 KRS-------------LSLSHRGRSKKGVFSSLKGLDTLTKRGHEKKHSLSQDLSE-LSCCSYSG 781

  Fly   678 LPEKLLTSGVYLPGTRTLQGDPLVTVDAACVASAGINCYEMATLLLYYSTIPERSSCPFTTPATP 742
            ||:...:.|          |..|.:|........|       :|...||.:|...|
Zfish   782 LPQVFDSDG----------GAQLNSVTHCSFKRLG-------SLTNVYSIVPPEGS--------- 820

  Fly   743 GTTATINGHKSTEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQIGN 807
                          |....::.......|..||.|:|:   ||.| .|:|::.::
Zfish   821 --------------QWDCSRESTTCIYMPNCQTVTVLL---KSHH-TVMDVLAET---------- 857

  Fly   808 CEILAVCPDSSLLQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVKFISVDQVTTSVAPSQL 872
            |.::.:.|.:..|:|              :....|..|.|:||..:|::.:..|::..|
Zfish   858 CMLVGLDPSTHCLRL--------------RRFTGDKMEISTPSAHELLQGLPYDELEVS------ 902

  Fly   873 PAGLLPSVPALSTLPGISQRHHDATKWREFFAQLEAFQRQCSTAGGRLVSALSEIRAADLQGLPT 937
                                            ||:.:
Zfish   903 --------------------------------QLQVY---------------------------- 907

  Fly   938 RRQLYGQHRALSRALMDSQLHSLRKMGAGQLARLQDLARAITSAPGSPRMTSQPQSSSSSSSGAF 1002
                              .||..|....|      |...|:|.
Zfish   908 ------------------TLHMSRPSLTG------DFGFAVTG---------------------- 926

  Fly  1003 LNVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALEDEISEVTSWMGSDGAENL 1067
             .||:   |:...::.:||                          |.|.::
Zfish   927 -YVDS---LKNSHIIVSEV--------------------------LPDGLA-------------- 947

  Fly  1068 QKFSQLHWDNESQLRTQEQEFEKYYFIAMKHLAKGRDL------HAAALKVSVLSESANNLKSAL 1126
              ||                         |.|..|.::      ..::|.::::....|.
Zfish   948 --FS-------------------------KGLRPGDEIVVLNGCEVSSLDLTLIQTLFNE----- 980

  Fly  1127 DQFAQKLELAHERYDAAGRLLHLLTQHQRENAAREELQRLAEQLGATSLLEKHWPVMRKTHTLPV 1191
                |.|:|..:|                                       |.|      ::|.
Zfish   981 ----QNLQLTAKR---------------------------------------HLP------SIPH 996

  Fly  1192 ASSTPAAAAGKRVSYRCSSASRSSFEGGPSSSNCSCWRESRNLEDMDQMEEEEEEQDHDCDGGDG 1256
            .|.|    |..:..||....:|   :...||::.||..|:.::.|..|.......|.|
Zfish   997 DSDT----ANVQSPYRDIHQNR---QRAKSSTDVSCVTETLSVNDGLQDSYHNHRQVH------- 1047

  Fly  1257 EEQQSKIADSGLGVCDNCERNPKLARICSCQSLNEKSHDELLEDE--------CFDRPSKRYMDI 1313
               .||..|:                :||.....::....|:|.:        ...||..|:|
Zfish  1048 ---HSKSVDT----------------VCSLYHSYQEGSGGLMESQEEQTGPEPAILRPCPRHM-- 1091

  Fly  1314 MHSPMEANAHLQCHGSSLELPKLEELSCLDPKIQKTLLLIMREMIGTERDYVRSLYYVIENYIDE 1378
                           |:.|            :::|    :::|::.||:.||:.|..:.|.|:..
Zfish  1092 ---------------SATE------------RLRK----VIQELVDTEKSYVKDLNCLFEIYLKP 1125

  Fly  1379 LLREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELE----------------RYERNPLKVGAA 1427
            |.:|..  ..:.:...:||::.::.:|... ||..||                ::::....:|.:
Zfish  1126 LQKETF--LTQDEMESLFGSLPEMLDFQKV-FLQTLEEKISSSPNFNTFETPVQFKKLLFSLGGS 1187

  Fly  1428 FLEMESKFYLYALYNKN---------KPKSDTLLSEYGSSFFKPKQMQLQDKLDLASYLLKPVQR 1483
            ||.....|.||:.:..|         :.|:|....|    |...:....|....|.|||:|||||
Zfish  1188 FLYYAEHFKLYSGFCANHIKVQKVLERAKTDRSFKE----FLDARNPTKQHSTTLESYLIKPVQR 1248

  Fly  1484 MGKYALLLQQLV--------------KACKGVEGAALQEIAADVEELQRAEEMVKFQLRHG---N 1531
            :.||.|||::||              :|.|.:|     ::|:.:.|:|:..|      .:|   :
Zfish  1249 VLKYPLLLRELVSLTDTDSEEHYHLTEALKEME-----KVASHINEMQKIYE------DYGAVFD 1302

  Fly  1532 DLLAMDSLRDCDVNVKEQGRLLRQNEFLVW------QGRGGKKTLRQVFLFEELVLF-----SKA 1585
            .|:|..|..:.:|.....|:.|..:. ::|      .||..|.....||:|:..|:.     :|.
Zfish  1303 QLVAEQSGTEKEVTEISMGQFLMHSS-VIWLNPFPSLGRMKKDPELTVFVFKRAVILLYRENNKV 1366

  Fly  1586 RR-----FPDHKNLDIYIYKNSIKTSDIGLTAHTGDSATKFEIW-------FRKRKPDDTWMLQC 1638
            |:     ...|.:.|.:.::..|..|  .|....|::|....:|       .::.:|:..:.|..
Zfish  1367 RKKMVTSRSAHGDQDSFKFRWLIPLS--ALQVRLGNTADTDCLWELIHTKSQKEGRPETVFQLCS 1429

  Fly  1639 MSEDIKNAWTEEISKLLWKQAKRNREVRLAEMSSMGIGSK--PCLDIRPSNNQI-SDRSIPLAQL 1700
            .:.:.|....:.:..:|.:..:||  ::..|.|......:  |.....|::.:| |.|:..||
Zfish  1430 STPECKVNIIKVMRSILRENLRRN--MQRVEQSERTCKKRLTPLRKSLPASARIGSSRAFWLA-- 1490

  Fly  1701 NKTPKLRHSEPGKGSMRRPNSLISESSLSSGTSTTSGSSISGGSSSGHNDGGGRHSLHFGK-LPG 1764
             |.|......|....:|.  ||....|.|:.:.|||.:|......|....|....|:...: ..|
Zfish  1491 -KQPSNDSPVPSNDPLRL--SLADSDSASASSGTTSSASAPLDPPSQQQSGLRESSIISDEDEDG 1552

  Fly  1765 AHSASSTNCSATLE-------LINET-QALKLAKSPSGGGI-----KGADTAADGGNTSGNGNGN 1816
            :..|..|:....:|       |..|| ||......|..|.:     |.:...|...........:
Zfish  1553 SEPADWTDAPNAIEVQFRKLGLTEETSQAAAPRVQPESGEVDTPERKASLKRAHFAVVKRKPARD 1617

  Fly  1817 GNAFRSSK------RNHKRSTTIVSQLSMES 1841
            .|:.|.|:      |.|..|..|.::::..|
Zfish  1618 TNSLRRSQVALLNMRRHSHSLDIPAEIAAHS 1648

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	PX_domain	15..>52	CDD:295365	6/21 (29%)
		RhoGEF	1353..1532	CDD:279015	53/220 (24%)
		PH_puratrophin-1	1524..1663	CDD:270062	30/164 (18%)
		PH	1562..1655	CDD:278594	20/109 (18%)
tiam2a	XP_009291563.1	PH1_Tiam1_2	488..613	CDD:269937	26/134 (19%)
		UBQ	829..904	CDD:320785	22/140 (16%)
		PDZ_signaling	906..986	CDD:238492	23/233 (10%)
		RhoGEF	1100..1289	CDD:214619	49/200 (25%)
		PH2_Tiam1_2	1291..1458	CDD:269957	33/177 (19%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910