DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pura and arhgef40

DIOPT Version :9

Sequence 1:	NP_996016.2	Gene:	Pura / 2768948	FlyBaseID:	FBgn0035802	Length:	1904	Species:	Drosophila melanogaster
Sequence 2:	XP_001919604.4	Gene:	arhgef40 / 100151026	ZFINID:	ZDB-GENE-110623-2	Length:	1752	Species:	Danio rerio

Alignment Length:	1825	Identity:	405/1825 - (22%)
Similarity:	635/1825 - (34%)	Gaps:	566/1825 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly   212 LIRNEPAPTTI-------------AERESEQALAEQEEELLEVEDLTEGSNGSYTDALPIECSLS 263
            |::::|.||.:             |:|:|    ...:||    :||....|.:..:| .:||...
Zfish   198 LLQDKPDPTDLSSGGLGLGWGGSSAQRDS----WSWDEE----DDLPPDGNEADIEA-ALECLRR 253

  Fly   264 ASIAEEQQQGVDELEPPKEEEVPLETELTEPSSTPPPQSNPEGIPAPERFRLTADVDATLARLID 328
            :|.......|..:.           .||.|      |:..|:|...|::            |.::
Zfish   254 SSDGHLVADGAGDY-----------VELLE------PRGGPDGGADPKQ------------RYLE 289

  Fly   329 SH---LSHIYPVYWARTRAILIQQARERLVCGF--------DGSLVRFEQRFLCKFAQIAATLRT 382
            .|   .:...|: ..|.:||.:::.:.|   |:        .|||||.:.....|...::.:
Zfish   290 MHGICKTKTLPL-CRRGKAIRLRKGKGR---GYGRTESGARGGSLVRRDSNNNGKDGVVSCS--- 347

  Fly   383 AHQIGESWLWHPGWPLATSNG-ALVLQLS------DAEIAHALRPSELYLCIKLESTESDADCKP 440
                          .||||.. ..|:.|.      ...|.||...:...:.::..|...|...|.
Zfish   348 --------------DLATSRPLPRVIDLGRQRRSYSPSILHADEGARDPVGLECRSDCMDNPAKE 398

  Fly   441 RLYAV------------WR-------SSRK----------------EESVQSHCIDHENPGELPL 470
            |...|            |.       |||:                :.|..|||...:   ::..
Zfish   399 RKPGVGKERDGTGGTLHWEECQRKKISSRRCSTDASDANSLGDGQQDRSQSSHCGCDD---KILA 460

  Fly   471 SVLQL--EMSTAAAALMASDCGELPQLLQNLLVSVERSIERVSLNELQMPMHHDRGEDSLDEANN 533
            |.|.:  :::|:.....|.|.|.:|       :|.:...|..: .||..|..  ....||.:::
Zfish   461 STLDIKEDIATSENQSNALDDGAIP-------LSADGKTEDTN-KELAEPSR--TRTRSLQDSS- 514

  Fly   534 NGCNNNLLGSPKCALRR---SDLITKNKLFNSRYMLRRLTNRQDSMNSTSSGN-SNVSDRSSSSS 594
               .:|.|||..||.||   ||...:.|:               .:|...||. ..:.::....|
Zfish   515 ---PSNALGSSDCAKRRNKHSDTTGEEKM---------------DINLPPSGTVGAIGEKQVGKS 561

  Fly   595 SSSSSSAGEELLT----------------------------------------------SHS--- 610
            .:|....|:|:.|                                              |||
Zfish   562 ENSVCPRGKEVKTGGFRAPRRKRKAKGGKGKAKSNGRVQQKGKGDQIISKNQTKATQPISHSSQS 626

  Fly   611 -EA-KMRSNSVGNEASGASTSPALPLYCLGNSFPHIDSDEEASDSIGPGKH--HSLETCETELLP 671
             || ::.:||.|.|||....:.|......|.|.....|.:.:.:.:|..:.  |...|.     |
Zfish   627 EEAPQLSTNSTGCEASSDDKTDAKSKAKDGESQSCTSSPDHSQNEVGLSQFAAHVESTS-----P 686

  Fly   672 SSSIAELPEKLLTSG-VYLPGTRTLQGDPLVTVDAACVASAGINCYEMATLLLYYSTIPERSSCP 735
            .:.:.:|...||.|| :.|.||....|..||..::. :...|....||..:|..|.||
Zfish   687 PTHLKDLDLNLLQSGKLILTGTMDRLGRALVVTESH-LPEDGWTTNEMIKVLSCYYTI------- 743

  Fly   736 FTTPAT--PGTTATINGHKSTEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSL 798
             |.|..  .|.|..::..||...:                              ||:     .:|
Zfish   744 -TRPMVKEKGLTVIVDSRKSAPSE------------------------------LCL-----SAL 772

  Fly   799 RLLAKQIGNCEILAVCPDSSLLQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVKFISVDQV 863
            :|..:|      .|...||.|:.                      .|.|..|....::.|.:..|
Zfish   773 KLFKEQ------AATGLDSVLIL----------------------TEESDESTQPYLEGIEMHTV 809

  Fly   864 TTSVAPSQLPAGLLPSVPALSTLP----GISQRHHDATKWREFFAQLEAFQRQCSTAGGRLVSAL 924
            ..:        |:|........||    |.....||  .|..|...||....:|.:|...|..||
Zfish   810 RGT--------GILQQFVDKQHLPREMGGDFNHSHD--DWLTFRLSLEQLTERCESALTLLGDAL 864

  Fly   925 SEIRAADLQG-LPTRRQLYGQHRALSRALM-DSQLHSLRKMGAGQLARLQDLARAITSAPGSPRM 987
            ..:....|.. :.|......:||.|...:: |.:|..|::.|...||.|         |.|
Zfish   865 QSMETEPLPDCIKTVPLSVDKHRQLMTGILTDQRLTELQQRGGAWLAGL---------ANG---- 916

  Fly   988 TSQPQSSSSSSSGAFLNVDAACKLRKVTL-LFNEVDRAAQRLEQLTEQRRERLRQLTRQRALEDE 1051
            ||.....|.....||          .||. |::.||.|..||.:.:.||...|..|.|..||.|:
Zfish   917 TSGLAQKSPDCKAAF----------AVTSDLYDSVDDALHRLVRSSNQRSRDLEALGRLAALVDK 971

  Fly  1052 ISEVTSWMGSDGAENLQKFSQLHWD---NESQLRTQEQEFEKYYFIAMKHLAKGRDLHAAALKVS 1113
            :.:.     ....|.:|...:.:.|   :.|:|..::.:|..:...||       |||:..|.:.
Zfish   972 LDKC-----ERDVEQIQDQLKDYKDPPLSLSRLTLKQHKFRSFRESAM-------DLHSETLVLL 1024

  Fly  1114 VLSESANNLK-SALDQFAQKL--------ELAH---ERY---DAAGRLLHLLTQHQR-------- 1155
            ...||.:.|. |.|....:||        :::|   :|:   |...|||..||:..:
Zfish  1025 AEVESWSELDWSGLAAVLEKLPPVREKVRDMSHCLSDRWTQLDNTQRLLSTLTEASQWCDVVSSS 1089

  Fly  1156 ----------------ENAAREELQRLAEQLGATSLLEKHWPVMRKTHTLPVASSTPAAAAGKRV 1204
                            ..:..::.:.||..||..:||:. |     :.||.....|.|....:.:
Zfish  1090 SPSSSSTSPLSSLPPIPPSRFQDARALALDLGGGALLDL-W-----SQTLERYQKTLAEFKSRIL 1148

  Fly  1205 SYRCSSASRSSFE---GG----PSSSNCSCWRESRNLEDMDQMEEEEEEQDHDCDGGD------- 1255
            .....|:.....|   ||    |:.|:.|      :|.|:    |.|.|.|. |.||:
Zfish  1149 QAERGSSVAQGQEPDLGGRGRTPAPSSTS------SLGDL----EGEIEPDW-CPGGEGGMQSWG 1202

  Fly  1256 ----------------GEEQQSKIADSGLG-----VCDNCERNPKLARICSCQSLNEKSHDELLE 1299
                            |||::.:..:.|.|     :....::||..|      .|..|.
Zfish  1203 SLASLFRPQNCSTLKIGEEKKKEGVNPGGGKFLQNLLHPAKKNPTDA------PLPPKP------ 1255

  Fly  1300 DECFDRPSKRY----MDIMHSPMEANAHLQ----CHGSSLELPKLEELSCLDPKI---------- 1346
                  |.||:    :..:.:|..|.|..:    ..|.|..|..|...:..||.:
Zfish  1256 ------PRKRHPSFDLQALLAPRRAGATSKPTESTSGQSSPLSWLGRKAMTDPILTAGVAAAVPG 1314

  Fly  1347 --------------------------------------------------------QKTLLLIMR 1355
                                                                    |..|.|...
Zfish  1315 WRDPAAGGEAGGGGVLIRGVEVSSKEVADHTGFTRQHVLLSRTERGTGLERAGVTAQSKLYLQWC 1379

  Fly  1356 EMIGTERDYVRSLYYVIENYIDELLREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELERYERN 1420
            .::.|||.||..|..|.|||:..|...|:|..|||:...:|.|.:.:..||:...|..:|.....
Zfish  1380 RLVSTERQYVAVLKGVEENYLPLLESPDVPSALRGKAESLFCNWKSLSAFHSQLLLPAMEGALSQ 1444

  Fly  1421 PLKVGAAFLEMESKFYLYALYNKNKPKSDTLLSEYGSSFFKPKQMQLQDKL----DLASYLLKPV 1481
            .|:....|.:.:.:|.||:.|.:.:|::|.|:....:.|||   |:|...|    .....|..|:
Zfish  1445 TLQQTDCFTKYKDQFLLYSQYIRMRPETDALIISQAADFFK---MKLCSSLVPAPPFPQCLSAPL 1506

  Fly  1482 QRMGKYALLLQQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNV 1546
            ||:.:|...|::|.....|.:.|.               .::|...|||.||.|.:.:..|.:.|
Zfish  1507 QRLQQYTQALEELAGLNPGSDSAL---------------SIIKHAHRHGEDLRASELISGCPIPV 1556

  Fly  1547 KEQGRLLRQNEFLVWQGRGGKKT-LRQVFLFEELVLFSKARRFPDHKNLDIYIYKNSIKTSDIGL 1610
            .|:|.|:||.|..|..|...||| :|.|||::..::|:| .:.|:..| .:|.:|:||||.::||
Zfish  1557 AERGDLVRQGELCVCGGGRRKKTGVRNVFLYQNFMIFTK-HKTPNPGN-SVYSFKHSIKTGEMGL 1619

  Fly  1611 TAHTGDSATKFEIWFRK-RKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREVRLAEMSSMG 1674
            |...|:...:||:|.|: .:..|...||..|...:.:||.:|::|||..|..|.|:.|.|...||
Zfish  1620 TQSVGEDGLRFEVWVRQASRTRDCLTLQTASHQDRESWTHDIAQLLWTHAMHNTELCLKESLCMG 1684

  Fly  1675 IGSKPCLDIRPSNNQISDRSIPLAQLNKTPKLRHSEPGK-GSMRR--PNSLISESSLSSGTSTTS 1736
            |.||..||:..:.:...|.|   :.||...:...|:... |||:.  |.|....|:..:|.:.:|
Zfish  1685 ISSKLLLDVTGAQSAELDSS---SSLNDRVQSSCSDSSSVGSMKEDSPASQTRRSAEHTGHTQSS 1746

  Fly  1737 GSSIS 1741
            ..|.|
Zfish  1747 SPSTS 1751

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	PX_domain	15..>52	CDD:295365
		RhoGEF	1353..1532	CDD:279015	50/182 (27%)
		PH_puratrophin-1	1524..1663	CDD:270062	53/140 (38%)
		PH	1562..1655	CDD:278594	33/94 (35%)
arhgef40	XP_001919604.4	RhoGEF	1381..1519	CDD:295373	43/140 (31%)
		PH-like	1534..1673	CDD:302622	53/140 (38%)
		PH	1562..1661	CDD:278594	37/100 (37%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0689
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D263447at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR45845
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	4	3.970