DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pura and mcf2l2

DIOPT Version :9

Sequence 1:	NP_996016.2	Gene:	Pura / 2768948	FlyBaseID:	FBgn0035802	Length:	1904	Species:	Drosophila melanogaster
Sequence 2:	XP_021335410.1	Gene:	mcf2l2 / 100149630	ZFINID:	ZDB-GENE-100812-1	Length:	1284	Species:	Danio rerio

Alignment Length:	1444	Identity:	302/1444 - (20%)
Similarity:	490/1444 - (33%)	Gaps:	432/1444 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   634 LYCLGNSFP--HIDSDEEASDSIGPGKHHSLETC-ETELLPSSS-----------IAELPEKLLT 684
            ||..|.||.  .|.:.|..|      .|..|..| ||||....:           ..::.|:|..
Zfish    16 LYSQGASFTLVRISAGEMES------YHRFLGVCLETELATRRNEIMQQEIRPLLAVDIIEQLHR 74

  Fly   685 SGVYLPGTRTLQGDPLVTVDAACVASAGIN---CYEMATLLLYYSTIPERSSCPFTTPATPGTTA 746
            ....|.|.|..:|.|::|..    ..:|.|   ..:...::.|.::||...:            |
Zfish    75 QFALLSGGRGQEGSPIITFP----EFSGFNEVSDEDFINVVTYLTSIPSMDA------------A 123

  Fly   747 TINGHKSTEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQIGNCEIL 811
            :|.                          |.|::...|.:....|..:.   |:.....||.:::
Zfish   124 SIG--------------------------FIIILDRRKDKWNSAIASLA---RIAGSFPGNLQLV 159

  Fly   812 AVCPDSSLLQLCNGQQQQKLQQQQQQNNNSDHPEPSSP-----SPVDLVKFISVDQVTTSVAPSQ 871
            .|...|..||........:|.:        |..:...|     |..||..:|...|:|..
Zfish   160 LVLKPSRFLQRAIADVSMRLHR--------DDFKLKVPIVMLNSLSDLHGYIDKGQLTCD----- 211

  Fly   872 LPAGLLPSVPALSTLPGISQRHHDATKWREFFAQLEAFQRQCSTAGGRLVSALSEIRAADL-QGL 935
                          |.|.....|  ::|......:|.|.....:....|....:::...:| ..:
Zfish   212 --------------LGGTLDYCH--SQWIHHRTAIENFALTVKSTAQMLQQFGTDLAETELPNDV 260

  Fly   936 PTRRQLYGQHRALSRALMDSQLHSLRKMGAGQLARLQDLARAITSAPGSPRMTSQPQSSSSSSSG 1000
            ...:.|...|......|.| :|....|.|...|:.::|.|                    :.:..
Zfish   261 QCTKDLLITHTEKHDKLKD-ELKLAVKQGNTLLSCIKDQA--------------------TKAES 304

  Fly  1001 AFLNVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALEDEISEV--------TS 1057
            ..||.|.......|..|..::|......||...:...:|.|..:.|..|.:..||        .:
Zfish   305 HILNPDEVENQTTVERLLAQLDETENAFEQFWSRHHLKLEQCLQLRHFEQDFKEVKVSLDGLSEA 369

  Fly  1058 WMG-SDGAENLQKFSQLHWD------------NESQLRTQEQEFEKYYFIAMKHLA--------- 1100
            .:| ||..:.:.:..||..:            .::||:.|..:    ..|...|.|
Zfish   370 LLGLSDTGDCVARVEQLLGELKTLEDKAQECLEKAQLQAQHGD----QLIQSNHYAIDSIRPKCV 430

  Fly  1101 --------------KGRDLHAAALKVSVLSESAN----------------------NLKSALDQF 1129
                          |.||:...:|::....:..|                      ..::||...
Zfish   431 ELRRICDDFSNEAKKKRDVLTKSLEILKRIDEVNQWCESGVYLLASQAVDRCQTQEGAEAALQDI 495

  Fly  1130 AQKLELAHERYDAAGRLLHLLTQHQRENAAREELQRLAEQLGATSL------LEKHWPVMRKTHT 1188
            .:.::.|.|:     :|.||...:.:......||.:...|....:|      .||....::|..|
Zfish   496 ERYMKTAKEQ-----QLSHLKELNNQYEVVLSELLKSKVQTALKTLNDVQEMFEKRHASLKKLST 555

  Fly  1189 ------LPVA---SSTPAAAAGK------RVSYRCSSASRSSFEGGPSSSNCSCWRESRNLEDMD 1238
                  .|||   .|:|...:.|      ..|:| .:...||....||.:..:..:..|..:...
Zfish   556 KQTRPVQPVAPRPESSPKRPSPKIPHPTPPTSHR-KTTDNSSTNRQPSDAELAKRKNIRKAKGGI 619

  Fly  1239 QMEEEEEEQDHDCDGGDGEEQQSKIADSGLGVCDNCERNPKLARICSCQSLNEKSHDELLEDECF 1303
            ::|...|       ||.|....         :..|.|....|:        |.:||
Zfish   620 KIEVMHE-------GGQGGSSH---------ILMNNETEESLS--------NRRSH--------- 651

  Fly  1304 DRPSKRYMDIMHSPMEANAHLQCHGSSLELPKLEELSCLDPKIQKTLLLIMREMIGTERDYVRSL 1368
                                                             ||.|:|.|||.||..|
Zfish   652 -------------------------------------------------IMNELIETERLYVEEL 667

  Fly  1369 YYVIENYIDELLRED------IPQPLRGQRNVIFGNIEKIFEFHNSHFLGELERYERNPLKVGAA 1427
            ..:||.|...|  :|      ||..|..::.|:|||:.:|::||...||.|||.....|..||..
Zfish   668 QSIIEGYAAAL--DDPELIYLIPPSLENKKEVLFGNLPEIYQFHQKIFLKELENSAEKPELVGTC 730

  Fly  1428 FLEMESKFYLYALYNKNKPKSDTLLSEYGSS-FFKPKQMQLQDKLDLASYLLKPVQRMGKYALLL 1491
            ||:.:.:..:|..|.:|||:|:.|..:.|.| ||:..|.:|..||.|.:||||||||:.||.|:|
Zfish   731 FLKRKEELQIYEKYCQNKPRSEALWRQCGDSMFFQECQKRLDHKLSLDAYLLKPVQRITKYQLML 795

  Fly  1492 QQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNVKEQGRLLRQN 1556
            ::::|..|..||.|         ||:.|:..|...::..||.:...::...:.::.:.|:||.|.
Zfish   796 KEMLKCSKNPEGTA---------ELEEAQATVLDIIKSVNDSMHQIAITGFEGSLNDLGKLLMQG 851

  Fly  1557 EFLVW----QGRGGKKTL-------RQVFLFEELVLFSKARR--FPDHKNLDIYIYKNSIKTSDI 1608
            .|.||    :|....|.|       |.:||:.:::||.|.|.  ...|:....|.:|:|:|...:
Zfish   852 SFNVWTDHKKGHSKVKDLARFKPMQRHLFLYHKMILFCKKREEMTDGHEKSPSYSFKHSLKMGSV 916

  Fly  1609 GLTAHTGDSATKFEIWFRKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREV-RLAEMSS 1672
            |:|.:......|||||:..|  ::.:::|..|.|:||.|..||.|:|..|.:..||. :|.:..:
Zfish   917 GITENVKGDIKKFEIWYNGR--EEVYIIQAPSMDVKNTWVSEIRKVLTSQLEACREASQLHQKIT 979

  Fly  1673 MGIGSKPCLDIRPSNNQISDRSIPLAQLNKT---PKLRHSEPGKGS------------------M 1716
            ..|...|..::|.:..:.||.|.|.....::   |.:|.......|                  :
Zfish   980 ENIYHAPMRNMRKTALRQSDSSSPETGYRRSDSGPNMRQKRADTSSSLGSERAALPRKRFTLQGL 1044

  Fly  1717 RRPNSLISE---------------SSLSSGTSTTSGSSISGGSSSGHNDGGGRHSLHFGKLPGAH 1766
            ....||.:|               |::.|.|:..:.:.:|....|      .||.:.....|..:
Zfish  1045 SNRRSLPAEPAPKETEVTRRFSLASTVGSATAPRTKAPLSSSLKS------KRHQIKSDPTPFGY 1103

  Fly  1767 SASSTNCSATLELINETQALKLAKSPSGGGIKGA------------------------------- 1800
            ..::.......|.:.::...: .||...||.|.|
Zfish  1104 EGATKTRGFKKETMRDSLPAE-RKSKGSGGSKPAVPRSASQPGWSQRRLPSMDAEDFETIPSSAE 1167

  Fly  1801 --DTAADGGNTSGNGNGNGNAFRSSKRNHKRSTTIVSQLSMESGIVSDMCV-TPDQEQSAAESSR 1862
              ..::||.:...|.||:.:.::             .||:.||....|:.| ..|..|...||..
Zfish  1168 ELSISSDGEDEGHNKNGDNDRYQ-------------VQLTYESCTAQDLSVEAGDFVQFLEESEN 1219

  Fly  1863 SSW-----STSTTG 1871
            ..|     .|..||
Zfish  1220 GLWLVKNLRTQKTG 1233

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	PX_domain	15..>52	CDD:295365
		RhoGEF	1353..1532	CDD:279015	72/185 (39%)
		PH_puratrophin-1	1524..1663	CDD:270062	43/151 (28%)
		PH	1562..1655	CDD:278594	32/101 (32%)
mcf2l2	XP_021335410.1	SEC14	77..217	CDD:214706	35/211 (17%)
		SPEC	348..554	CDD:238103	34/214 (16%)
		RhoGEF	649..826	CDD:238091	74/245 (30%)
		PH_Dbs	833..964	CDD:269934	40/132 (30%)
		Mating_C	932..>1122	CDD:315419	38/197 (19%)
		SH3_DBS	1188..1242	CDD:212791	14/59 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910