DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pura and mcf2la

DIOPT Version :9

Sequence 1:	NP_996016.2	Gene:	Pura / 2768948	FlyBaseID:	FBgn0035802	Length:	1904	Species:	Drosophila melanogaster
Sequence 2:	XP_021332781.1	Gene:	mcf2la / 100148439	ZFINID:	ZDB-GENE-090312-122	Length:	1221	Species:	Danio rerio

Alignment Length:	1333	Identity:	293/1333 - (21%)
Similarity:	492/1333 - (36%)	Gaps:	299/1333 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly   676 AELPEKLLTSGVYLPGTRTLQGDPLVTVDAACVASAGINCYEMATLLLYYSTIPERSSCPFTTPA 740
            |::...|.....:|.|.|...|.|:: :.........:...|...:|.|.::||..||...
Zfish    68 ADISPDLRKQFAFLSGGRGQNGSPII-IFPEYPEFGELEEQEFHNVLTYLTSIPSLSSTGI---- 127

  Fly   741 TPGTTATINGHKSTEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQI 805
              |....|:              ::|.:....:.|.                     ||:.....
Zfish   128 --GFILVID--------------RRQDRWASVKGTL---------------------LRIAGSFP 155

  Fly   806 GNCEILAVCPDSSLLQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVKFISVDQVTTSVAPS 870
            ||.:::.|...::|.       |:.:.....:.|..|.     ...|.::...||.::.:.:..|
Zfish   156 GNLQLVLVLRPTTLF-------QRTISDILFKFNKDDF-----KMKVPVIMLSSVTELHSYIDRS 208

  Fly   871 QLPAGLLPSVPALSTLPGISQRHHDATKWREFFAQLEAFQRQCSTAGGRLVSALSEIRAADLQGL 935
            ||          ...|.|..:..|:  ||......:|.|..........|.|..:|:...:|.
Zfish   209 QL----------TQELGGTQEYCHE--KWISHRTAIEGFALMVKKTAQTLQSFGTELAETELP-- 259

  Fly   936 PTRRQLYGQHRALSRALMDSQLHSLRKMGAGQLARLQDLARAITSAPGSPRMTSQPQSSSSSSSG 1000
                   ....|.|..|   .:|:.:|.     ...:||..|:..........::|.......|
Zfish   260 -------NDVEATSNLL---TVHTEKKD-----KMKEDLRIALCQGGRLLESINEPLVKDPDYS- 308

  Fly  1001 AFLNVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALEDEISEVTSWM------ 1059
              :|.|....|..|..|..::|......:...::.:.:|.|..:.|..|....||.:.:
Zfish   309 --MNHDELENLATVQRLLGQLDETETAFDDFWDKHQTKLEQCLQLRHFEQNFREVRAHLDLVYDR 371

  Fly  1060 ---------GSDGAENLQKFSQLHWDNESQLRTQ--------EQEFEKYYFIAMKHLAKGRDLHA 1107
                     ....||::.|....|.:..|::..|        :|..|..::.....|.|..:|.:
Zfish   372 LSGFSEVGISPAHAEHILKELTNHEERASEVMDQALSLASDADQLIEASHYAVDSILPKCSELRS 436

  Fly  1108 AALKVSVL--SESANNLKSALDQFAQKLELAHERYDAAGRLLHLLTQHQRENAAREELQRLAEQL 1170
            ...::|.:  ::.|...||.  :..|.||.|.:.||..   ::||        |.:.:.:...|.
Zfish   437 VCEEISTILRAKKAYLFKSV--ELHQCLEKATKWYDDG---IYLL--------ASQPVDKCQSQD 488

  Fly  1171 GATSLLEKHWPVMRKTHTLPVASSTPAAAAGKRVSYRCSSASRSSFEGGPSSSNCSCWRESRNLE 1235
            ||.|.|::   :.|...|                   .:....:...|        .||
Zfish   489 GAESALQE---IERYLDT-------------------ANQHKLTDLNG--------IWR------ 517

  Fly  1236 DMDQMEEEEEEQDHDCDGGDGEEQQSKIADSGLGVCDNCE-RNPKLARICSCQSLNEK---SHDE 1296
            |.|.:..:           |..:|..|:....|.:.:..| |...|.::.:.|:...:   ...|
Zfish   518 DYDSVLTQ-----------DFRDQVDKVFQKQLSMQEMFEKRRVSLKKLAAKQTRPVQPVAPRPE 571

  Fly  1297 LLEDECFDRPSKRYMDIMHSPMEANA-----HLQCHGSSLELPKLEELSCLDPKIQKTLLL--IM 1354
            .:.......|:.|...|....:..|.     |||..|||      ...|..|.:....:|.  :|
Zfish   572 AIIKTPVSSPAHREKSIEGDVINGNCKKGELHLQNEGSS------RHPSVSDEEENFAVLRRHVM 630

  Fly  1355 REMIGTERDYVRSLYYVIENYIDEL----LREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELE 1415
            .|::.|||.||..|..|:|.|..|:    :...||..|..:::::|||:.:|::||...||.|||
Zfish   631 NELLETERAYVEELLCVLEGYAAEMDNPAMSHLIPNTLLHKKDILFGNMSEIYQFHKKTFLRELE 695

  Fly  1416 RYERNPLKVGAAFLEMESKFYLYALYNKNKPKSDTLLSEYGS-SFFKPKQMQLQDKLDLASYLLK 1479
            .|...|..||..|||..:...:|..|.:|||:|::|..:... :||:..|.:|:.||.|.|||||
Zfish   696 AYTDYPELVGQCFLERMTDLQIYEKYCQNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLK 760

  Fly  1480 PVQRMGKYALLLQQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDV 1544
            ||||:.||.|||::|:|..||.||.         |:||.|...:...|:..||.:.:.::...:.
Zfish   761 PVQRITKYQLLLKELLKYSKGCEGE---------EDLQEALSSILGILKAVNDSMHLIAITGYEG 816

  Fly  1545 NVKEQGRLLRQNEFLVW----QGRGGKKTL-------RQVFLFEELVLFSKAR--RFPDHKNLDI 1596
            |:.:.|||:.|..|.||    :|....|.|       |.:||.|:.:||.|.|  ....::....
Zfish   817 NLSDLGRLMMQGSFSVWTEHKKGHAKVKDLARFKPMQRHLFLHEKALLFCKKREENGEGYEKAPS 881

  Fly  1597 YIYKNSIKTSDIGLTAHTGDSATKFEIWFRKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKR 1661
            |.:|:|:..|.:|:|.:......|||||...|  ::.:::|..:.:||.||..||.|:|.:|.|.
Zfish   882 YSFKHSLSMSAVGITENAKGDNKKFEIWCNSR--EEVYIVQASTPEIKTAWVNEIRKVLTRQLKA 944

  Fly  1662 NREVRLAEMSSMGIGSKPCLDIRPSNN-------QISDRSIPLAQLNKTPKLRHSEPGKGSMRRP 1719
            .||      :|....|.....:.||:.       :.:.:|:......||.... ::|...::.:.
Zfish   945 CRE------ASQQKSSDQISPLTPSSTGLTLSPFKTNPKSLKKGDEKKTEPTT-TDPSSAALFKN 1002

  Fly  1720 NSLISESSLSS----------------GTSTTSGSSISGGSSSGHNDGGGRHSLHFG-KLPGAH- 1766
            |..:.:.::.|                |.|.......|...|..|..........|| :..|.|
Zfish  1003 NDKLKDETVVSPNTERAAVAKKRFTLQGFSNLKSQKGSSPPSPDHKTKRQSDPTPFGFRDAGPHI 1067

  Fly  1767 --------SASS---------TNCSATLELINETQALKLAKSPSGGGIKGADTAADGGNTSGNGN 1814
                    |.||         |..|.:::...|......|:.|     ..:|...:||.....|.
Zfish  1068 TLTRVRWLSTSSLLQTKRRGFTKASLSMDASEENDGYSSAEDP-----MNSDPEDEGGKKLSPGK 1127

  Fly  1815 GNGNAFRSSKRNHKRSTTIVSQLSMESG--------------IVSDMCVTPDQEQSAAE------ 1859
            ....|      :|.:|..  .:|:::||              .|.::..:.:....||.
Zfish  1128 YVVVA------DHDKSGP--QELTVKSGDTVQLVREGDDGRWFVRNLRTSKEGWVPAANLLTLIG 1184

  Fly  1860 SSRSSWSTSTTGASGASTTSASTVILRRYRSYA 1892
            .|:||.|.|::..||:...|.|:.....|.|::
Zfish  1185 ESKSSQSLSSSEGSGSGNLSTSSSCSETYTSFS 1217

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	PX_domain	15..>52	CDD:295365
		RhoGEF	1353..1532	CDD:279015	72/183 (39%)
		PH_puratrophin-1	1524..1663	CDD:270062	45/151 (30%)
		PH	1562..1655	CDD:278594	31/101 (31%)
mcf2la	XP_021332781.1	SEC14	73..219	CDD:214706	34/209 (16%)
		SPEC	386..552	CDD:321951	41/225 (18%)
		RhoGEF	626..803	CDD:238091	72/185 (39%)
		PH_Dbs	810..941	CDD:269934	40/132 (30%)
		SH3_DBS	1127..1181	CDD:212791	9/61 (15%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910