Protein Alignment Pura and mcf2la

DIOPT Version :10

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: XP_073810125.1
• Gene: mcf2la / 100148439
• ZFINID: ZDB-GENE-090312-122
• Length: 1242
• Species: Danio rerio
• Alignment Length: 1354
• Identity: 299/1354 - (22%)
• Similarity: 497/1354 - (36%)
• Gaps: 320/1354 - (23%)

  Fly   676 AELPEKLLTSGVYLPGTRTLQGDPLVTVDAACVASAGINCYEMATLLLYYSTIPERSSCPFTTPA 740
            |::...|.....:|.|.|...|.|:: :.........:...|...:|.|.::||..||...
Zfish    68 ADISPDLRKQFAFLSGGRGQNGSPII-IFPEYPEFGELEEQEFHNVLTYLTSIPSLSSTGI---- 127

  Fly   741 TPGTTATINGHKSTEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQI 805
              |....|:              ::|.:....:.|.                     ||:.....
Zfish   128 --GFILVID--------------RRQDRWASVKGTL---------------------LRIAGSFP 155

  Fly   806 GNCEILAVCPDSSLLQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVKFISVDQVTTSVAPS 870
            ||.:::.|...::|.       |:.:.....:.|..|.     ...|.::...||.::.:.:..|
Zfish   156 GNLQLVLVLRPTTLF-------QRTISDILFKFNKDDF-----KMKVPVIMLSSVTELHSYIDRS 208

  Fly   871 QLPAGLLPSVPALSTLPGISQRHHDATKWREFFAQLEAFQRQCSTAGGRLVSALSEIRAADLQGL 935
            ||          ...|.|..:..|:  ||......:|.|..........|.|..:|:...:|.
Zfish   209 QL----------TQELGGTQEYCHE--KWISHRTAIEGFALMVKKTAQTLQSFGTELAETELP-- 259

  Fly   936 PTRRQLYGQHRALSRALMDSQLHSLRKMGAGQLARLQDLARAITSAPGSPRMTSQPQSSSSSSSG 1000
                   ....|.|..|   .:|:.:|.     ...:||..|:..........::|.......|
Zfish   260 -------NDVEATSNLL---TVHTEKKD-----KMKEDLRIALCQGGRLLESINEPLVKDPDYS- 308

  Fly  1001 AFLNVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALEDEISEVTSWM------ 1059
              :|.|....|..|..|..::|......:...::.:.:|.|..:.|..|....||.:.:
Zfish   309 --MNHDELENLATVQRLLGQLDETETAFDDFWDKHQTKLEQCLQLRHFEQNFREVRAHLDLVYDR 371

  Fly  1060 ---------GSDGAENLQKFSQLHWDNESQLRTQ--------EQEFEKYYFIAMKHLAKGRDLHA 1107
                     ....||::.|....|.:..|::..|        :|..|..::.....|.|..:|.:
Zfish   372 LSGFSEVGISPAHAEHILKELTNHEERASEVMDQALSLASDADQLIEASHYAVDSILPKCSELRS 436

  Fly  1108 AALKVSVL--SESANNLKSALDQFAQKLELAHERYDAAGRLLHLLTQHQRENAAREELQRLAEQL 1170
            ...::|.:  ::.|...||.  :..|.||.|.:.||..   ::||        |.:.:.:...|.
Zfish   437 VCEEISTILRAKKAYLFKSV--ELHQCLEKATKWYDDG---IYLL--------ASQPVDKCQSQD 488

  Fly  1171 GATSLLEKHWPVMRKTHTLPVASSTPAAAAGKRVSYRCSSASRSSFEGGPSSSNCSCWRESRNLE 1235
            ||.|.|::   :.|...|                   .:....:...|        .||
Zfish   489 GAESALQE---IERYLDT-------------------ANQHKLTDLNG--------IWR------ 517

  Fly  1236 DMDQMEEEEEEQDHDCDGGDGEEQQSKIADSGLGVCDNCE-RNPKLARICSCQSLNEK---SHDE 1296
            |.|.:..:           |..:|..|:....|.:.:..| |...|.::.:.|:...:   ...|
Zfish   518 DYDSVLTQ-----------DFRDQVDKVFQKQLSMQEMFEKRRVSLKKLAAKQTRPVQPVAPRPE 571

  Fly  1297 LLEDECFDRPSKRYMDIMHSPMEANA-----HLQCHGSSLELPKLEELSCLDPKIQKTLLL--IM 1354
            .:.......|:.|...|....:..|.     |||..|||      ...|..|.:....:|.  :|
Zfish   572 AIIKTPVSSPAHREKSIEGDVINGNCKKGELHLQNEGSS------RHPSVSDEEENFAVLRRHVM 630

  Fly  1355 REMIGTERDYVRSLYYVIENYIDEL----LREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELE 1415
            .|::.|||.||..|..|:|.|..|:    :...||..|..:::::|||:.:|::||...||.|||
Zfish   631 NELLETERAYVEELLCVLEGYAAEMDNPAMSHLIPNTLLHKKDILFGNMSEIYQFHKKTFLRELE 695

  Fly  1416 RYERNPLKVGAAFLEMESKFYLYALYNKNKPKSDTLLSEYGS-SFFKPKQMQLQDKLDLASYLLK 1479
            .|...|..||..|||..:...:|..|.:|||:|::|..:... :||:..|.:|:.||.|.|||||
Zfish   696 AYTDYPELVGQCFLERMTDLQIYEKYCQNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLK 760

  Fly  1480 PVQRMGKYALLLQQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDV 1544
            ||||:.||.|||::|:|..||.||.         |:||.|...:...|:..||.:.:.::...:.
Zfish   761 PVQRITKYQLLLKELLKYSKGCEGE---------EDLQEALSSILGILKAVNDSMHLIAITGYEG 816

  Fly  1545 NVKEQGRLLRQNEFLVW----QGRGGKKTL-------RQVFLFEELVLFSKAR--RFPDHKNLDI 1596
            |:.:.|||:.|..|.||    :|....|.|       |.:||.|:.:||.|.|  ....::....
Zfish   817 NLSDLGRLMMQGSFSVWTEHKKGHAKVKDLARFKPMQRHLFLHEKALLFCKKREENGEGYEKAPS 881

  Fly  1597 YIYKNSIKTSDIGLTAHTGDSATKFEIWFRKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKR 1661
            |.:|:|:..|.:|:|.:......|||||...|  ::.:::|..:.:||.||..||.|:|.:|.|.
Zfish   882 YSFKHSLSMSAVGITENAKGDNKKFEIWCNSR--EEVYIVQASTPEIKTAWVNEIRKVLTRQLKA 944

  Fly  1662 NREVRLAEM---------SSMGIGSKPCLDIRP-------------------------SNNQISD 1692
            .||....:.         ||.|:...| ....|                         :|:::.|
Zfish   945 CREASQQKSSDQISPLTPSSTGLTLSP-FKTNPKSLKKGDEKKTEPTTTDPSSAALFKNNDKLKD 1008

  Fly  1693 RSI--PLAQLNKTPKLRHSEPGKGSMRRPNSLISESSLSSGTSTT--------SGSSISGGSSSG 1747
            .::  |..:.....|.|.:..|..:::....|:   :.|.|.|.|        .|||   ..|..
Zfish  1009 ETVVSPNTERAAVAKKRFTLQGFSNLKSQKELL---AASGGKSLTLPMVTLCHPGSS---PPSPD 1067

  Fly  1748 HNDGGGRHSLHFG-KLPGAH---------SASS---------TNCSATLELINETQALKLAKSPS 1793
            |..........|| :..|.|         |.||         |..|.:::...|......|:.|
Zfish  1068 HKTKRQSDPTPFGFRDAGPHITLTRVRWLSTSSLLQTKRRGFTKASLSMDASEENDGYSSAEDP- 1131

  Fly  1794 GGGIKGADTAADGGNTSGNGNGNGNAFRSSKRNHKRSTTIVSQLSMESG--------------IV 1844
                ..:|...:||.....|.....|      :|.:|..  .:|:::||              .|
Zfish  1132 ----MNSDPEDEGGKKLSPGKYVVVA------DHDKSGP--QELTVKSGDTVQLVREGDDGRWFV 1184

  Fly  1845 SDMCVTPDQEQSAAE------SSRSSWSTSTTGASGASTTSASTVILRRYRSYA 1892
            .::..:.:....||.      .|:||.|.|::..||:...|.|:.....|.|::
Zfish  1185 RNLRTSKEGWVPAANLLTLIGESKSSQSLSSSEGSGSGNLSTSSSCSETYTSFS 1238

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	31/171 (18%)
		RhoGEF	1353..1524	CDD:459876	71/175 (41%)
		PH_puratrophin-1	1524..1663	CDD:270062	45/151 (30%)
mcf2la	XP_073810125.1	None