Protein Alignment Pura and plekhg4b

DIOPT Version :10

• Sequence 1: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Sequence 2: XP_073780503.1
• Gene: plekhg4b / 100009641
• ZFINID: ZDB-GENE-101112-5
• Length: 1612
• Species: Danio rerio
• Alignment Length: 1768
• Identity: 475/1768 - (26%)
• Similarity: 704/1768 - (39%)
• Gaps: 525/1768 - (29%)

  Fly   322 TLARLIDSHLSHIYP--------VYWARTRAILIQQARERLVCGFDGSLVRFEQRFLCKFAQIAA 378
            :|.:.|:|.||.:||        |.|.....:..|...:.|.|..|         ||....:|..
Zfish    25 SLDQCIESVLSALYPPFSSSAATVLWQLFGVVERQYRGDGLRCFID---------FLLPAKRILH 80

  Fly   379 TLRTAHQIGESW----LWHPGWPLATSNGALVLQLSDAEIAHALRPSELYLCIKLESTESDADCK 439
            |||  ||....:    |:|.||||.. :..:||||:...... ||..:.||
Zfish    81 TLR--HQACLRFKGVLLYHEGWPLCL-HEKVVLQLAPLHKVR-LRQGDFYL-------------- 127

  Fly   440 PRLYAVWRSSRKEESVQSHCIDHENP--GELPLSVLQ---------LEMSTAAAALMASDCGELP 493
                .|....||...:...|:.....  .|:|:|...         |:..|....|
Zfish   128 ----QVVPLGRKSAKLVLKCLSGSGQAIAEVPVSESMYGSVFTPDFLQNVTRDRNL--------- 179

  Fly   494 QLLQNLLVSVERSIERVSLNELQMPMHHDRGEDSLDEANNNG----------C----------NN 538
            |.|||.|:|....:.|.....:..|:.....||:|.:|.|:|          |          :.
Zfish   180 QPLQNCLLSTGSVVYRTPWKNVINPLLVTSTEDALMQARNSGVVGLRGQISPCSTHGSTGTLSSR 244

  Fly   539 NLLGSPKC----ALRRS-----------------------------------DLITKN------- 557
            :.||....    ||.||                                   ||...|
Zfish   245 DSLGGESTVSEPALSRSLTDMGIGSLHREDSDSQKSPDSSSIRGHKLLSFSTDLSNPNMRKRHPR 309

  Fly   558 --KLFNSRYMLRR--LTNRQDSMNSTSSGNSNV--------------------------SDRSSS 592
              ..|.||.:.|:  :...|:.||..||..|.:                          .|.|.:
Zfish   310 DSAAFESRRLFRKSYMEALQNPMNLGSSSESILEEPSFASVPKESMAPVRKICARGWLGGDESRN 374

  Fly   593 SSSSSSSSAGEELLTSHSEAKM--RSNS-----VGNEA---SGASTSPALPLYCLGN-------- 639
            .|.:.:.|...||..:...:|.  |:|:     .|:.|   ||.|.| ..|...:..
Zfish   375 GSLAGNPSKNAELSPNERRSKSLERTNNKTLQVKGHRARSSSGGSNS-GFPKKLINGYGFRFGKL 438

  Fly   640 ----SFPHID--SDEE----ASDSIGPGKHHSLETCETELLPSSS-----IAELPEKLLTSG-VY 688
                :||.::  |.:|    .|:..|....:|.:.|.:...|:.|     |::|.::||.|| :.
Zfish   439 ELEAAFPGLERRSSKENSGVCSEWSGRRSSNSGDECVSAAPPAVSPSALMISQLDQELLNSGALI 503

  Fly   689 LPGTRTLQGDPLVTVDAAC---VASAGINCYEMATLLLYYSTIPERSSCPFTTPATPGTTATING 750
            |||.....|..:|.|  .|   |.|:......::.||.||
Zfish   504 LPGNVERGGRAVVQV--CCSKQVWSSDCTDQIISVLLCYY------------------------- 541

  Fly   751 HKSTEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQIGNCEILAVCP 815
                                                        |.|||...:::|    |.|..
Zfish   542 --------------------------------------------CSSLRKEKRELG----LTVLV 558

  Fly   816 DSSLLQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVDLVKFISVDQVTTSVAPS-QLPAGLLPS 879
            |:...|.........|.|.|          .|.|:.:..|.|:...:....|... ..|..:|.|
Zfish   559 DARRQQTVPSTLISALSQFQ----------TSVPNALYSVLFLVEKEAAVKVERDVTFPYEVLTS 613

  Fly   880 VPALSTLPGISQRHHDATK---------------WREFFAQLEAFQRQCSTAGGRLVSALSEIRA 929
            :.||       |:|.|:|:               :..|..::|.|...||.|...|.|::..:.
Zfish   614 LKAL-------QKHIDSTQLTTDLDGTFPYDHSHYINFRQKIEPFASSCSAAISFLQSSIETLN- 670

  Fly   930 ADLQGLPTRR---QLYGQHRALSRALM-DSQLHSLRKMGAGQLARLQDLARAITSAPGSPRMTSQ 990
             ::..|.|.:   ::.||.::|.:.:: |::|:.||..|...||||:                .:
Zfish   671 -NISNLETAQEVSEVIGQQKSLMKNILDDTELNRLRLEGGTFLARLR----------------KE 718

  Fly   991 PQSSSSSSSGAFLNVDAACKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALEDEISEV 1055
            ....:.:...|   ||.      |:.|:|:||....:|..|:.:..:.|..|...|.::::..|:
Zfish   719 EMCENHNYRDA---VDL------VSALYNQVDEEVHKLVILSNKSLQNLETLLELRTIQEKQEEI 774

  Fly  1056 TSWMGSDGAENLQKFSQLHWDNESQLRTQEQEFEKYYFIAMKHLAKGRDLHAAALKVSVLSESAN 1120
            ..|...:...:|...     |:.|...:..|:..|...:.|:...:.::..:..|:.|..|.|..
Zfish   775 KRWFYVESERHLAPL-----DSFSLSLSSIQDMSKSLELFMQEAREQQNKASVLLRESDSSSSLL 834

  Fly  1121 NLKSALDQFAQKLE-----------------------------LAHERYDAAGRLLHLLTQHQRE 1156
            .|...:....|:.|                             |...|.|.....|.:|.....|
Zfish   835 ELNQQIISVLQRCETRRSQLHVLHNLYEFYQSANEWMLQCQEYLCRLRVDGDAAALQMLQDFHSE 899

  Fly  1157 ---------NAAREELQRLAEQLGATSLLEKHWPVMRKT--------------HTLPVASSTPAA 1198
                     ::.::.||:| |..|........|...:.|              ||..|.||.|
Zfish   900 AQQFSPQNFSSFQQTLQQL-ESPGEEQRWRNIWRSCKHTQQQLEELMESSTPQHTTQVFSSPP-- 961

  Fly  1199 AAGKRVSYRCSSASRSSFEGG-----PSSSNCSCWRESRNLEDMDQMEEE---EEEQDHDCDGGD 1255
                           .|..||     |..|:|.      ..:|..|....   .|:.|.||....
Zfish   962 ---------------ESLNGGVSPDLPPLSSCV------EYDDAPQFPRSLSTAEDTDSDCTVDS 1005

  Fly  1256 GEEQQSKIADSGLGVCDNCERNPKLARICSCQSLNEKSH--------------DELLEDECFDRP 1306
            ...:..::            |...|.:|....|....:|              :.:..::...||
Zfish  1006 SPSEPHRL------------RKQPLKKIMKTLSYEPSAHHGYTGVYIRGLEVSNNVSAEKKLQRP 1058

  Fly  1307 SKRYMDIMHSPMEANAHLQCHGSSLELP-KLEELSCLDPKIQKTLLLIMREMIGTERDYVRSLYY 1370
            .      :.||:...:.      ||..| :.:....:..|:|.    ||.|||.|||:|||||.|
Zfish  1059 D------VKSPVLTRSR------SLSTPCRADHCHSVSSKVQH----IMDEMISTEREYVRSLIY 1107

  Fly  1371 VIENYIDELLREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELERYERNPLKVGAAFLEMESKF 1435
            |::.|..|:.|.|:||.|||:|::||||:||:.:||:.:||.||||...:||.|...||..|.:|
Zfish  1108 VVQQYFPEMERLDLPQDLRGKRSIIFGNLEKLSDFHSLYFLKELERCSHSPLTVSRCFLMHEEQF 1172

  Fly  1436 YLYALYNKNKPKSDTLLSEYGSSFFKPKQMQLQDKLDLASYLLKPVQRMGKYALLLQQLVKACKG 1500
            .:||||:||||:||.||:.:|::||:.||::|.||:|||||||||:|||.||||||:.|:|.|..
Zfish  1173 GMYALYSKNKPRSDALLTSHGNAFFRNKQVELGDKMDLASYLLKPIQRMSKYALLLKDLIKECSE 1237

  Fly  1501 VEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNVKEQGRLLRQNEFLVWQGRG 1565
            .:.   ||:|    :|:.|||||||||||||||||||::|.||||:||||:|..|:||:||.||
Zfish  1238 SQE---QELA----DLRTAEEMVKFQLRHGNDLLAMDAIRGCDVNLKEQGQLRCQDEFIVWCGR- 1294

  Fly  1566 GKKTLRQVFLFEELVLFSKARRFPDHKNLDIYIYKNSIKTSDIGLTAHTGDSATKFEIWFRKRKP 1630
             ||.||.|||||:|:||||.::.  ....||||||.|.||::||:|...|||..:||||||:||.
Zfish  1295 -KKYLRHVFLFEDLILFSKTKKI--EGGYDIYIYKQSFKTAEIGMTESVGDSGLRFEIWFRRRKT 1356

  Fly  1631 DDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREVRLAEMSSMGIGSKPCLDIRPSNNQISDRSI 1695
            .||::||..|.::|:.||..|.|:||:||.||||:|:.||.|||||:||.:||:||::.||||::
Zfish  1357 QDTFILQAASAEVKSVWTGVIGKILWRQALRNRELRMQEMVSMGIGNKPFMDIKPSDSAISDRAV 1421

  Fly  1696 PLAQLNKTPKLR---------HSEPGKGSMRRPNSLISESSLSSGTSTTSGSSISGGSSSGHNDG 1751
            .....:...:.|         ||.|    .:||:|.||.||.||.:|.:|.|.::...|..|...
Zfish  1422 DYTMKSSDSRTRTSIAVSSVDHSTP----FKRPHSTISNSSSSSSSSQSSSSLLNLHPSHTHPAQ 1482

  Fly  1752 GGRHSLHFGKLPGAH--------------------------SASSTNCSATLELIN-------ET 1783
            .....:|...:..:|                          .:||:.|:::..:.|       ..
Zfish  1483 IHPSHIHPSHIHPSHWPYDCIEEDELEHESSIQNSMIGESVESSSSQCTSSDSISNLSIITVQHH 1547

  Fly  1784 QALKLAKSPSGGG 1796
            .||.:.|..|.||
Zfish  1548 PALLMEKDSSDGG 1560

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	30/184 (16%)
		RhoGEF	1353..1524	CDD:459876	95/170 (56%)
		PH_puratrophin-1	1524..1663	CDD:270062	83/138 (60%)
plekhg4b	XP_073780503.1	None