Protein Alignment GLI3 and gli3

DIOPT Version :10

• Sequence 1: NP_000159.3
• Gene: GLI3 / 2737
• HGNCID: 4319
• Length: 1580
• Species: Homo sapiens
• Sequence 2: NP_991291.1
• Gene: gli3 / 403042
• ZFINID: ZDB-GENE-041111-162
• Length: 1553
• Species: Danio rerio
• Alignment Length: 1688
• Identity: 851/1688 - (50%)
• Similarity: 1041/1688 - (61%)
• Gaps: 243/1688 - (14%)

Human     1 MEAQSHSSTTTEKKKVENSIVKCS--TRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQ 63
            ||.|..:|::|| |::||.:...|  ||||:||||||||||||||:|.|..||.||||.|:.|..
Zfish     1 MEPQFQTSSSTE-KRMENVVGTNSAPTRTDISEKAVASSTTSNEDDSSGSPYHLERRNTISAQTD 64

Human    64 NVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLF 128
            .|....|||||||||:|||..|:|||:...|..:|:.::|||||:||||.|:||::|||..|..|
Zfish    65 VVNTSGKVSEEPSTSTDERLPLVKKELQSFLHRLADHTLPYRGTLFAMDLRSGYLDPHYTAPQFF 129

Human   129 PAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPTAASESPFSPP 193
            .|||.|||||.||.:|||.|:|||:|.|||..||::|||:.|:..|||||||||:..::|||:|.
Zfish   130 RAFHHPVPIDDRHTQGRYIYEPSPVPSLHMPPALAASPTFSDISLIRISPHRNPSMGTDSPFNPS 194

Human   194 HPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPS- 257
            ||||||||||||||||||||||:||.|||||.|..|.|::.||||||||||.|.||||||:|||
Zfish   195 HPYINPYMDYIRSLHSSPSLSMVSAARGLSPADTHHTGLTTAEYYHQMALLVGHRSPYADLIPSV 259

Human   258 AATAG--TGAIHMEYLHAMDSTRFSSPRLSARPSRKRTLSISP-LSDHSFDLQTMIRTSPNSLVT 319
            .:|.|  :.|:|||||.||:|:||.||||..||||||.:..|| |||.|||||.|||||||||||
Zfish   260 GSTTGPASSALHMEYLQAMESSRFPSPRLINRPSRKRPIPNSPSLSDPSFDLQAMIRTSPNSLVT 324

Human   320 ILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSL-GSAFGHSPPLIHP 383
            ||||||||||.|||||||||..|||||||.|...||:|.||||::|||..: ||||||||||:|.
Zfish   325 ILNNSRSSSSNSGSYGHLSAGTISPALSFAYPPTPVALQMHQQLISRQPGIVGSAFGHSPPLMHS 389

Human   384 APTFPTQRPIPGIPT-VLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPG 447
            |..|.||||:|.|.| .|.|.: .|..|..||:|||||||||||||||::||||:||:|:.|||.
Zfish   390 ALAFSTQRPVPAIQTSSLAPTE-RSIVSNDSQSKPTSESAVSSTGDPMNHKRSKLKPEEEPPSPR 453

Human   448 ARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKE 512
            |...|||..|.|||||||||:|.||||||:|||||||:||.||||||||||||||||||||||||
Zfish   454 AVSMQEQMNGMTLVKEEGDKEEGKQEPEVVYETNCHWDGCCREFDTQEQLVHHINNDHIHGEKKE 518

Human   513 FVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYV 577
            |||||.:||||||||||||||||||||||||||||||||||:|||||||||||||||||||||||
Zfish   519 FVCRWEECSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYV 583

Human   578 CEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQ 642
            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Zfish   584 CEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQ 648

Human   643 RGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSP 707
            ||:.:|||||        |.|.||..|     :|::.::.|||::||||||::|.|||||||.||
Zfish   649 RGETYPRPPP--------QPREPGATT-----DQKEYTHATSKQDECLQVKSIKTEKPMTSQSSP 700

Human   708 GGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAI-----------------DETPIMDSTIS 755
            |.||||||.||....:.:.|::|.:...|..|:::.:                 ::.||||||:|
Zfish   701 GSQSSCSSDQSINGPHVSQGVQLTVNSIGLAGEVAMLEDMENEEEDEENREGEKEDAPIMDSTVS 765

Human   756 TATTALA---LQARRNPA-GTKWMEHVKLERLKQVNGMFPRLNPILPPKAP----AVSPLIGNGT 812
            |||::.|   ||..|:.. ..:||||||:|||:||||..|||. .|||..|    |:..|.|.|
Zfish   766 TATSSAATIVLQTHRSVGRPLRWMEHVKMERLRQVNGAVPRLG-FLPPTPPRKGQAMPALTGKG- 828

Human   813 QSNNTCSLGGPMT--LLPGRSDLSGVDVTMLNMLN-RRDSSASTISSAYL-SSRRSSGISPCFSS 873
                .|.  |.||  .:|...:.|..::|:|::|: |||||.|..||||| ||||||||||||||
Zfish   829 ----QCL--GRMTSVRVPPCPEPSNTELTILSLLHERRDSSGSNTSSAYLSSSRRSSGISPCFSS 887

Human   874 RRSSEASQAEGRP----QNVSVADSYDPISTDASRRSSEASQSDGLPSL---------------- 918
            |.||:||.:|..|    .|:|..|||||||||||||||||||.||..|.
Zfish   888 RCSSQASLSEYSPHRHIHNLSATDSYDPISTDASRRSSEASQYDGGSSSAVVFGGSGVFKGSGMG 952

Human   919 ----LSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPG-VALPPVHAPRR 978
                |:|||||.|.||||||||||||||||||:|..::|||..||..|..:.| :.|||:...||
Zfish   953 SRGDLNLTPAQHYSLKAKYAAATGGPPPTPLPSMGHINLKTHFALKEDRKDTGQLTLPPLVRSRR 1017

Human   979 CSDGGAHG-------YGRRHLQPHDAPGHGVRRASDPV--RTGSEGLALPRVPRFSSL----SSC 1030
            ||| |||.       |.|:.......||...||||||.  :.......||:|..||||    ||.
Zfish  1018 CSD-GAHNSHDSQAVYQRKGTYFGGGPGKNDRRASDPTHHKQNLSSYGLPQVRHFSSLNNMTSSP 1081

Human  1031 NPPAMA-TSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADA---NLNDEDF 1091
            ||.:.. ::.|..||.|||.|..........| |||||||.|...||::.|:.|.   .:|:.
Zfish  1082 NPASYGYSNREDHSLNLQNSTCHVDSFQSGLH-SPCPPSIMEQSALENIFMNQDGLLLRVNEN-- 1143

Human  1092 LPDDVVQYLNSQNQAG---YEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEG 1153
            :..|.:|||:||:|..   .:.|.|...|.|.:      :|.:|       .||...:||   |.
Zfish  1144 IQSDFMQYLHSQDQESIIIQDNHRPIFNPADDQ------NFLSP-------QQQQEGVEQ---ED 1192

Human  1154 SKTDL-PIQWNEVSSGSADLSSSK---LKCG--PRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGG 1212
            ::.|| |||.|||||.|||::..:   .:||  ...:...:.|||....|||. |.|:
Zfish  1193 TRIDLMPIQSNEVSSASADVTIPRQPFQRCGRWSDQSGSVSNAFGRFGNMVVQ-QQPI------- 1249

Human  1213 YQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNR----QPVAPGALDGACG 1273
                 :..:.:...:|:       .|.|             || |.|.    |...|........
Zfish  1250 -----DFIDQHSALQHM-------GGIS-------------YG-CRNSEQHLQVAKPAQEKSRMK 1288

Human  1274 AGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAG 1338
            .||:..  .|:..|......|||.|...:..:.          |:.|....|.::  |..:|
Zfish  1289 TGIKTE--SSSTFQLESKHSNFGRPDFSHIFSA----------PIHQKCSQHTVV--SQSYP--- 1336

Human  1339 RPGQQMLGQISATSHINIY-QGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSL 1402
               ||  .||:.....|:: |.|.:|            ||.:....||..:...|.||.:...::
Zfish  1337 ---QQ--NQITNQDSSNLFLQRPGNC------------SLLLAYRPQPAVNTSYSTRQDINSHNV 1384

Human  1403 ------ALQSGQLSDTSQTCRVNGIKMEMKGQPHPL----CSNLQNYSGQFYDQTVGFSQQDTKA 1457
                  :|.:...:..|.:.:|:.:|::   |.:.|    ||.:|.....|.|:.  |..:::|.
Zfish  1385 QSCHPQSLNNRLGTSCSLSNQVSVVKLD---QVNHLQINSCSTIQQQKPVFIDKC--FDPEESKL 1444

Human  1458 GSFSISDASCLLQGTSAKNSE----LLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDG-DHSS 1517
            ....:.  |||::...|:...    |||.|..||||.|:...:   |.|.:...:|..|. |..|
Zfish  1445 SHAIVQ--SCLMESLMAEKERTSKVLLSSGVGQVTSKVERSPA---EKVGLGICSIGGDSYDQGS 1504

Human  1518 LMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ 1580
            ::              |||||:....|..|.::...|.||||.||:||||:|||||||||::|
Zfish  1505 VV--------------SSSRLSNSHDSFAFHSVDPGTNNMAISDMNSLLTTLAEESKFLAIIQ 1553

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
GLI3	NP_000159.3	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..79		47/79 (59%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	368..475		69/108 (64%)
		SFP1	<542..632	CDD:227516	88/89 (99%)
		C2H2 Zn finger	548..570	CDD:275368	20/21 (95%)
		C2H2 Zn finger	578..601	CDD:275368	22/22 (100%)
		C2H2 Zn finger	609..630	CDD:275368	20/20 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	620..728		64/107 (60%)
		Mediates interaction with DZIP1. /evidence=ECO:0000250|UniProtKB:Q61602	745..845		46/109 (42%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	863..918		41/58 (71%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	981..1042		26/74 (35%)
gli3	NP_991291.1	FOXP-CC	488..>522	CDD:465036	31/33 (94%)
		C2H2 Zn finger	524..546	CDD:275368	19/21 (90%)
		SFP1	<548..638	CDD:227516	88/89 (99%)
		C2H2 Zn finger	554..576	CDD:275368	20/21 (95%)
		C2H2 Zn finger	584..607	CDD:275368	22/22 (100%)
		C2H2 Zn finger	615..636	CDD:275368	20/20 (100%)