DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Myo81F and myo5b

DIOPT Version :9

Sequence 1:	NP_001303492.1	Gene:	Myo81F / 26067052	FlyBaseID:	FBgn0267431	Length:	2082	Species:	Drosophila melanogaster
Sequence 2:	XP_021324561.1	Gene:	myo5b / 567402	ZFINID:	ZDB-GENE-031219-7	Length:	1840	Species:	Danio rerio

Alignment Length:	1935	Identity:	471/1935 - (24%)
Similarity:	768/1935 - (39%)	Gaps:	580/1935 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 VGFADMTEMSEIDEIGINQNLNVRY-NSDIIYTYTGSILLAVNPYKTINIFNKQTISEYHTCKLG 71
            ||..|:|.:|.:.|..:..||.||: .|:.||||.|.:|:|:|||:.::|:.::.|:.|....:|
Zfish    69 VGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLHIYGEEVINAYSGQNMG 133

  Fly    72 DLSPHVFAVAEAAYTSIVDDNINQACVISGESGAGKTESTKFILQYLCTITSNVSSW-VQQQILE 135
            |:.||:|||||.|:..:..|..||:.::|||||||||.|.|:.:::..|:..:.:.. |::::|.
Zfish   134 DMDPHIFAVAEEAFKQMARDEKNQSIIVSGESGAGKTVSAKYAMRFFATVGGSANDTNVEEKVLA 198

  Fly   136 ANTILEAFGNAKTTRNDNSSRFGKFMQVCFDDNNCIKGCVILDYLLEQSRITFQSEGERNYHIMY 200
            ::.|:||.||||||||||||||||::|:.||....|.|..:..||||:||:.||:|.||||||.|
Zfish   199 SSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDRRYHIIGANMRTYLLEKSRVVFQAEEERNYHIFY 263

  Fly   201 QLVAQGQ----KNIGIANTFHLRPAEFYKYLNTSNSEKINMNLESKKFDAVTM--AFTVLQIPQF 259
            ||.|...    |::.:.:      ||.:.|  ||..|.|.:...:...|.|..  |.|:|.:.:.
Zfish   264 QLCASCSLPEFKDLTLTS------AEDFTY--TSLGENIFIEGVNDAEDLVKTREALTMLGVKEN 320

  Fly   260 VIDGVFKVISSILWLGNIEFI-DVDGEVTDLTRSDQEAISIISMLLGLSRCDFKKVLLIRQINVR 323
            ....:||:|:|||.|||:|.: :.|||...:.|:|.. :.....|||:.:...:..|..|::
Zfish   321 HQMSIFKIIASILHLGNVEIVQERDGESCHINRNDTH-LHHFCRLLGIEQEQMENWLCRRKL--- 381

  Fly   324 GNITEIPLKIK-----EALENRHALAKALYSKMFTWLVSKINNCTNPGQDDAKFLGVLDIFGFEN 383
              :|.....:|     :|:..|.||||.:|:.:|.|:|..||...:.......|:|||||:|||.
Zfish   382 --VTTSETYVKNMPHSQAVNARDALAKHIYAHLFDWIVEHINKSLHTSTKQHSFIGVLDIYGFET 444

  Fly   384 FSHNSFEQLCINYTNEKLHKFFNHYVFALEQSIYEHEGIYFNHVEFTDNTPCLEILEKPPRCVFK 448
            |..|||||.||||.||||.:.||.:||.|||..|..|.|.:..::|.||.||::::| ....:..
Zfish   445 FEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIE-AKLGILD 508

  Fly   449 LLTEQCHMPQGSDLAFLSNMHTEFESHPNYIKGSDRRHWE------TEFGIKHYAGLVIYAVGGF 507
            ||.|:|.:|:|:|..:...:         |.|.|...|:|      ..|.:.|:|..|.|...||
Zfish   509 LLDEECKVPKGTDQNWAQKL---------YSKHSGSGHFEKPRMSNKSFIVVHFADKVEYQCDGF 564

  Fly   508 VEKNRDVQQDVMFEYMSYSKDVFIKDLSTYQDIQ-----------FIN----SSYPRGSSKS-KC 556
            :|||||...:.....:..||...:.||  :.|.:           .||    ...|:|.::. :.
Zfish   565 LEKNRDTVYEEQINILKASKFQLVADL--FDDAKDAAPHPGSKTSRINVRPAKPAPKGHNREHRK 627

  Fly   557 TVSDNFRNQLQSLIDVLQNTKPWYVRCIKPNLQKRPNNYNSSLVLDQLKYLGVVDIIRIRREGFP 621
            ||...|||.|..|::.|..|.|.||||||||..|....::|...:.||:..||::.|||...|:|
Zfish   628 TVGTQFRNSLHLLMETLNATTPHYVRCIKPNDYKESFVFDSRRAVQQLRACGVLETIRISAAGYP 692

  Fly   622 IHLSHKDFIVRYKCLIKNKSFEYPQK--YLTNILEKNGMPTTEWQIGKSKIFLRNAAYELLETNR 684
            ...::.||..||:.|:..|......|  ...|:||.......::|.||:|||.|......||..|
Zfish   693 SRWTYPDFFSRYRVLMTKKDLSVGDKKQVCKNLLEILIKDPDKFQFGKTKIFFRAGQVAYLEKLR 757

  Fly   685 KD------------------------------------------------TLYKNAVIIQKNWKK 701
            .|                                                .|.:.|||.||.::.
Zfish   758 ADKFRFACIKIQKTVRGWLQRIRYRKIRKSAITLQRYGRGYLARRYAEMLRLTRAAVICQKQYRM 822

  Fly   702 YYIQKSFLRNKQAVLR-------------------------IQHAYRGWRLRIRFMRMRRSAIVI 741
            ..:::.:||.::||:.                         ||...|||.:|.::.|.|.:||||
Zfish   823 VQVRREYLRVRRAVITIQAFTRGMFIRRLYQEFLLHHKAMIIQKTVRGWLVRKKYQRSRYAAIVI 887

  Fly   742 QSRLRGVFAR-------------------------EVAAALREMKRVDEELKKRD---------- 771
            |...|.|.|:                         ::....::|....:|||.::
Zfish   888 QCFYRRVRAKRQLKQLKIEARSAEHFKKLNVGMENKIVQLQKKMDNQSKELKSQNENLAVAKTVL 952

  Fly   772 -----GIYEQLTSITTKTLADCE-RSIQEELSVLAHISDNIRASAKPLSDKNDSDSNTARSSI-- 828
                 .:.::|.::.|:.:|..: .|:||||..|........|..|.|.::..::.......:
Zfish   953 ETEVSKLSKELETLRTRQVAGTQMTSLQEELEKLRAELQEAHAHKKLLEEEFSNEKQGLEQRVEE 1017

  Fly   829 --KQNTV------NLDNMFDFLCESKGA----FENALIKEIND---NWNNLVK----------NL 868
              |:|||      .::.......:.:|.    .|:.|..|::|   .:.||||          ||
Zfish  1018 LEKENTVLKKEKEEMNRRIQTSTQDQGGDVSQKESRLQHELDDERQRYQNLVKEYSRLEQRYENL 1082

  Fly   869 DEEIGLCIHTEDSFDSQKTGKLFTTKTVRTLPEPTVPPPPPPVSISANLNSEPIYEAINLYKLTT 933
            .|::                     .:::..|.....|     |..::|.|:..|.:|:|.::..
Zfish  1083 QEDM---------------------SSMKFHPGHRRNP-----SNQSSLGSDSNYPSISLSEVGD 1121

  Fly   934 TKE-IKKEKYMLLDYKSIKDNSKKPSTSSVFVKYQ--IDERENRRKQRVEKKIHELTLIDIHKDI 995
            |.: |::.:.|.::        |.....|||:|.|  :.|.|..|| |::..:.::..:...|..
Zfish  1122 TDDTIQQVEEMGIE--------KAAMDLSVFMKLQKRVRELEQERK-RLQTNLDKVEELSKRKGS 1177

  Fly   996 HIDNSFYNILEFAENYFNTHDLSSDCNFISTPTQKGKSSDKTAKHEMTMFSK--SDKIP--TSHI 1056
            .           |:|..:..|:::|..:.|...|:.::.:|..|:::....|  :|:..  .|..
Zfish  1178 E-----------AKNSTSEDDINADLAYNSLKRQELETENKKLKNDLNELRKAVADRASENNSSN 1231

  Fly  1057 HMYDPENVVLSCSMFRELCKYMRGELNAERELQIIQYIVGLGIEREE---LRDEIFIQCIRQSRN 1118
            .:.|..|::||             :|.|..|        .|.:.:||   |:.:| :...||:.|
Zfish  1232 ELQDGYNLLLS-------------QLKAANE--------ELDVRKEEVLMLKTQI-VNSTRQTEN 1274

  Fly  1119 NPNIDWTDRIWIILCLSIVAFQPSKLLFRYFVSFIKKSMIELDGKLRQYSQWCFDNCKSTKVSTR 1183
            ..:|:..|                                        .|:|. ||........|
Zfish  1275 LNHINSND----------------------------------------VSEWT-DNKSPVNTEDR 1298

  Fly  1184 MYPPSSVEVAAMRRLGTIVCRFFFLDARTKAID---VHPTDTAGDAVQKLAEKLNLTTTDGWAIY 1245
            :..|:|                     :.||.|   ::.....|.|.|.|.:...|..|
Zfish  1299 VDAPAS---------------------KNKAQDWGYLNEDGELGLAYQGLKQVARLLET------ 1336

  Fly  1246 QNRPDGEEHIKSYDYLYDVISSWEMKQATYAGTKKTTNGSSSGENQFVFKKRLFKSTHELSQDPV 1310
            |.:....:|....:.|:..|                               .|.|...|..||.:
Zfish  1337 QLQTQTRQHRDELEALHTQI-------------------------------ELLKDNLEKKQDML 1370

  Fly  1311 EVSMLYSQAVH-SVVKKD---EFPVSEKVA------LQLAGLQAQVALGDPSNQPKPEYYSDINS 1365
            :         | |.:..|   ||.|.:::.      |.|..|..::    ..|:.|      :..
Zfish  1371 D---------HFSTLSPDALVEFSVQQEITRLTNENLDLKELVEKL----EKNEKK------LKK 1416

  Fly  1366 FLPIRISKTREQQFWIPILAQAHRQYGSSRNALTAKVLYLSCVMQYPLYGTTMFKVNYKGYWNLA 1430
            .|.|.:.|.:|.:     :.||.:    ||..||.:                 |.|..:..
Zfish  1417 QLRIYMKKVQELE-----VTQATK----SRPELTRQ-----------------FTVQRREK---- 1451

  Fly  1431 SNIILGIHYDGIMFINPEDKSVVFQFKYVDIESILLDPSDSFITISLNRSAQLLKTSLYGSSLIE 1495
                   .::|::....||:                              |.|:||      |:.
Zfish  1452 -------DFEGMLEYYKEDE------------------------------ALLVKT------LVT 1473

  Fly  1496 SQKCFVFETPQKNDIGSLIISYYPALSNWILNNLECSKKYKGTTKEDRLRLYQNVVLCRRHLVDM 1560
            ..|        .|.:.:.:    |.|..:||                        .:|.||...:
Zfish  1474 DMK--------PNAVSATV----PCLPAYIL------------------------FMCIRHADYI 1502

  Fly  1561 NIVKKPHDFGGFLRNTLRRLSKHRLEKLRSEVGSNTQEHGETYKGFSHTFWAFSKQQIPFCISTI 1625
            |..:|.|.......|.::::.|           .|..:...|      :||..:..::..|:...
Zfish  1503 NDDQKVHSLLTATINAIKKVLK-----------KNNDDFEMT------SFWLANASRLLHCLKQY 1550

  Fly  1626 SDQEEALMIQVFDSILTYSGLGSSGESISRAEDEHIRIIQSIMDKCMKK------ESLLNELYLQ 1684
            |. :||.|.|                 .|..::||          |:|.      ..:|::|.:|
Zfish  1551 SG-DEAFMTQ-----------------NSAKQNEH----------CLKNFDLAEYRQVLSDLSIQ 1587

  Fly  1685 LIKQTTDHPDANSRVNLKNWALLSVLCSVILPSMKAVRKYLIAHLKRCSS 1734
            :.:|.....:|    |::...:.::|.|..:||:..|:.  :.:..|.||
Zfish  1588 IYQQLVKVAEA----NMQPMIVSAMLESESIPSLAGVKP--MGYRNRSSS 1631

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Myo81F	NP_001303492.1	MYSc	8..685	CDD:214580	261/714 (37%)
		Motor_domain	23..673	CDD:277568	252/687 (37%)
		MyTH4	1091..1194	CDD:279165	17/105 (16%)
		B41	1202..1419	CDD:214604	39/229 (17%)
		UBQ	1202..1297	CDD:294102	13/97 (13%)
		FERM_M	1307..1419	CDD:278785	23/121 (19%)
		PH-like	1415..1523	CDD:302622	14/107 (13%)
		MyTH4	1664..1771	CDD:279165	16/77 (21%)
		UBQ	1780..1860	CDD:294102
		B41	1781..1992	CDD:214604
		FERM_B-lobe	1893..1984	CDD:271216
		PH-like	1988..2076	CDD:302622
myo5b	XP_021324561.1	Myosin_head	7..1371	CDD:332202	397/1494 (27%)
		MYSc_Myo5	83..746	CDD:276831	252/688 (37%)
		Myo5b_CBD	1461..1835	CDD:271261	50/294 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.910