Protein Alignment Myo81F and myo5b

DIOPT Version :10

• Sequence 1: NP_001303492.1
• Gene: Myo81F / 26067052
• FlyBaseID: FBgn0267431
• Length: 2082
• Species: Drosophila melanogaster
• Sequence 2: XP_021324561.1
• Gene: myo5b / 567402
• ZFINID: ZDB-GENE-031219-7
• Length: 1840
• Species: Danio rerio
• Alignment Length: 1935
• Identity: 471/1935 - (24%)
• Similarity: 768/1935 - (39%)
• Gaps: 580/1935 - (29%)

  Fly     8 VGFADMTEMSEIDEIGINQNLNVRY-NSDIIYTYTGSILLAVNPYKTINIFNKQTISEYHTCKLG 71
            ||..|:|.:|.:.|..:..||.||: .|:.||||.|.:|:|:|||:.::|:.::.|:.|....:|
Zfish    69 VGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLHIYGEEVINAYSGQNMG 133

  Fly    72 DLSPHVFAVAEAAYTSIVDDNINQACVISGESGAGKTESTKFILQYLCTITSNVSSW-VQQQILE 135
            |:.||:|||||.|:..:..|..||:.::|||||||||.|.|:.:::..|:..:.:.. |::::|.
Zfish   134 DMDPHIFAVAEEAFKQMARDEKNQSIIVSGESGAGKTVSAKYAMRFFATVGGSANDTNVEEKVLA 198

  Fly   136 ANTILEAFGNAKTTRNDNSSRFGKFMQVCFDDNNCIKGCVILDYLLEQSRITFQSEGERNYHIMY 200
            ::.|:||.||||||||||||||||::|:.||....|.|..:..||||:||:.||:|.||||||.|
Zfish   199 SSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDRRYHIIGANMRTYLLEKSRVVFQAEEERNYHIFY 263

  Fly   201 QLVAQGQ----KNIGIANTFHLRPAEFYKYLNTSNSEKINMNLESKKFDAVTM--AFTVLQIPQF 259
            ||.|...    |::.:.:      ||.:.|  ||..|.|.:...:...|.|..  |.|:|.:.:.
Zfish   264 QLCASCSLPEFKDLTLTS------AEDFTY--TSLGENIFIEGVNDAEDLVKTREALTMLGVKEN 320

  Fly   260 VIDGVFKVISSILWLGNIEFI-DVDGEVTDLTRSDQEAISIISMLLGLSRCDFKKVLLIRQINVR 323
            ....:||:|:|||.|||:|.: :.|||...:.|:|.. :.....|||:.:...:..|..|::
Zfish   321 HQMSIFKIIASILHLGNVEIVQERDGESCHINRNDTH-LHHFCRLLGIEQEQMENWLCRRKL--- 381

  Fly   324 GNITEIPLKIK-----EALENRHALAKALYSKMFTWLVSKINNCTNPGQDDAKFLGVLDIFGFEN 383
              :|.....:|     :|:..|.||||.:|:.:|.|:|..||...:.......|:|||||:|||.
Zfish   382 --VTTSETYVKNMPHSQAVNARDALAKHIYAHLFDWIVEHINKSLHTSTKQHSFIGVLDIYGFET 444

  Fly   384 FSHNSFEQLCINYTNEKLHKFFNHYVFALEQSIYEHEGIYFNHVEFTDNTPCLEILEKPPRCVFK 448
            |..|||||.||||.||||.:.||.:||.|||..|..|.|.:..::|.||.||::::| ....:..
Zfish   445 FEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIE-AKLGILD 508

  Fly   449 LLTEQCHMPQGSDLAFLSNMHTEFESHPNYIKGSDRRHWE------TEFGIKHYAGLVIYAVGGF 507
            ||.|:|.:|:|:|..:...:         |.|.|...|:|      ..|.:.|:|..|.|...||
Zfish   509 LLDEECKVPKGTDQNWAQKL---------YSKHSGSGHFEKPRMSNKSFIVVHFADKVEYQCDGF 564

  Fly   508 VEKNRDVQQDVMFEYMSYSKDVFIKDLSTYQDIQ-----------FIN----SSYPRGSSKS-KC 556
            :|||||...:.....:..||...:.||  :.|.:           .||    ...|:|.::. :.
Zfish   565 LEKNRDTVYEEQINILKASKFQLVADL--FDDAKDAAPHPGSKTSRINVRPAKPAPKGHNREHRK 627

  Fly   557 TVSDNFRNQLQSLIDVLQNTKPWYVRCIKPNLQKRPNNYNSSLVLDQLKYLGVVDIIRIRREGFP 621
            ||...|||.|..|::.|..|.|.||||||||..|....::|...:.||:..||::.|||...|:|
Zfish   628 TVGTQFRNSLHLLMETLNATTPHYVRCIKPNDYKESFVFDSRRAVQQLRACGVLETIRISAAGYP 692

  Fly   622 IHLSHKDFIVRYKCLIKNKSFEYPQK--YLTNILEKNGMPTTEWQIGKSKIFLRNAAYELLETNR 684
            ...::.||..||:.|:..|......|  ...|:||.......::|.||:|||.|......||..|
Zfish   693 SRWTYPDFFSRYRVLMTKKDLSVGDKKQVCKNLLEILIKDPDKFQFGKTKIFFRAGQVAYLEKLR 757

  Fly   685 KD------------------------------------------------TLYKNAVIIQKNWKK 701
            .|                                                .|.:.|||.||.::.
Zfish   758 ADKFRFACIKIQKTVRGWLQRIRYRKIRKSAITLQRYGRGYLARRYAEMLRLTRAAVICQKQYRM 822

  Fly   702 YYIQKSFLRNKQAVLR-------------------------IQHAYRGWRLRIRFMRMRRSAIVI 741
            ..:::.:||.::||:.                         ||...|||.:|.::.|.|.:||||
Zfish   823 VQVRREYLRVRRAVITIQAFTRGMFIRRLYQEFLLHHKAMIIQKTVRGWLVRKKYQRSRYAAIVI 887

  Fly   742 QSRLRGVFAR-------------------------EVAAALREMKRVDEELKKRD---------- 771
            |...|.|.|:                         ::....::|....:|||.::
Zfish   888 QCFYRRVRAKRQLKQLKIEARSAEHFKKLNVGMENKIVQLQKKMDNQSKELKSQNENLAVAKTVL 952

  Fly   772 -----GIYEQLTSITTKTLADCE-RSIQEELSVLAHISDNIRASAKPLSDKNDSDSNTARSSI-- 828
                 .:.::|.::.|:.:|..: .|:||||..|........|..|.|.::..::.......:
Zfish   953 ETEVSKLSKELETLRTRQVAGTQMTSLQEELEKLRAELQEAHAHKKLLEEEFSNEKQGLEQRVEE 1017

  Fly   829 --KQNTV------NLDNMFDFLCESKGA----FENALIKEIND---NWNNLVK----------NL 868
              |:|||      .::.......:.:|.    .|:.|..|::|   .:.||||          ||
Zfish  1018 LEKENTVLKKEKEEMNRRIQTSTQDQGGDVSQKESRLQHELDDERQRYQNLVKEYSRLEQRYENL 1082

  Fly   869 DEEIGLCIHTEDSFDSQKTGKLFTTKTVRTLPEPTVPPPPPPVSISANLNSEPIYEAINLYKLTT 933
            .|::                     .:::..|.....|     |..::|.|:..|.:|:|.::..
Zfish  1083 QEDM---------------------SSMKFHPGHRRNP-----SNQSSLGSDSNYPSISLSEVGD 1121

  Fly   934 TKE-IKKEKYMLLDYKSIKDNSKKPSTSSVFVKYQ--IDERENRRKQRVEKKIHELTLIDIHKDI 995
            |.: |::.:.|.::        |.....|||:|.|  :.|.|..|| |::..:.::..:...|..
Zfish  1122 TDDTIQQVEEMGIE--------KAAMDLSVFMKLQKRVRELEQERK-RLQTNLDKVEELSKRKGS 1177

  Fly   996 HIDNSFYNILEFAENYFNTHDLSSDCNFISTPTQKGKSSDKTAKHEMTMFSK--SDKIP--TSHI 1056
            .           |:|..:..|:::|..:.|...|:.::.:|..|:::....|  :|:..  .|..
Zfish  1178 E-----------AKNSTSEDDINADLAYNSLKRQELETENKKLKNDLNELRKAVADRASENNSSN 1231

  Fly  1057 HMYDPENVVLSCSMFRELCKYMRGELNAERELQIIQYIVGLGIEREE---LRDEIFIQCIRQSRN 1118
            .:.|..|::||             :|.|..|        .|.:.:||   |:.:| :...||:.|
Zfish  1232 ELQDGYNLLLS-------------QLKAANE--------ELDVRKEEVLMLKTQI-VNSTRQTEN 1274

  Fly  1119 NPNIDWTDRIWIILCLSIVAFQPSKLLFRYFVSFIKKSMIELDGKLRQYSQWCFDNCKSTKVSTR 1183
            ..:|:..|                                        .|:|. ||........|
Zfish  1275 LNHINSND----------------------------------------VSEWT-DNKSPVNTEDR 1298

  Fly  1184 MYPPSSVEVAAMRRLGTIVCRFFFLDARTKAID---VHPTDTAGDAVQKLAEKLNLTTTDGWAIY 1245
            :..|:|                     :.||.|   ::.....|.|.|.|.:...|..|
Zfish  1299 VDAPAS---------------------KNKAQDWGYLNEDGELGLAYQGLKQVARLLET------ 1336

  Fly  1246 QNRPDGEEHIKSYDYLYDVISSWEMKQATYAGTKKTTNGSSSGENQFVFKKRLFKSTHELSQDPV 1310
            |.:....:|....:.|:..|                               .|.|...|..||.:
Zfish  1337 QLQTQTRQHRDELEALHTQI-------------------------------ELLKDNLEKKQDML 1370

  Fly  1311 EVSMLYSQAVH-SVVKKD---EFPVSEKVA------LQLAGLQAQVALGDPSNQPKPEYYSDINS 1365
            :         | |.:..|   ||.|.:::.      |.|..|..::    ..|:.|      :..
Zfish  1371 D---------HFSTLSPDALVEFSVQQEITRLTNENLDLKELVEKL----EKNEKK------LKK 1416

  Fly  1366 FLPIRISKTREQQFWIPILAQAHRQYGSSRNALTAKVLYLSCVMQYPLYGTTMFKVNYKGYWNLA 1430
            .|.|.:.|.:|.:     :.||.:    ||..||.:                 |.|..:..
Zfish  1417 QLRIYMKKVQELE-----VTQATK----SRPELTRQ-----------------FTVQRREK---- 1451

  Fly  1431 SNIILGIHYDGIMFINPEDKSVVFQFKYVDIESILLDPSDSFITISLNRSAQLLKTSLYGSSLIE 1495
                   .::|::....||:                              |.|:||      |:.
Zfish  1452 -------DFEGMLEYYKEDE------------------------------ALLVKT------LVT 1473

  Fly  1496 SQKCFVFETPQKNDIGSLIISYYPALSNWILNNLECSKKYKGTTKEDRLRLYQNVVLCRRHLVDM 1560
            ..|        .|.:.:.:    |.|..:||                        .:|.||...:
Zfish  1474 DMK--------PNAVSATV----PCLPAYIL------------------------FMCIRHADYI 1502

  Fly  1561 NIVKKPHDFGGFLRNTLRRLSKHRLEKLRSEVGSNTQEHGETYKGFSHTFWAFSKQQIPFCISTI 1625
            |..:|.|.......|.::::.|           .|..:...|      :||..:..::..|:...
Zfish  1503 NDDQKVHSLLTATINAIKKVLK-----------KNNDDFEMT------SFWLANASRLLHCLKQY 1550

  Fly  1626 SDQEEALMIQVFDSILTYSGLGSSGESISRAEDEHIRIIQSIMDKCMKK------ESLLNELYLQ 1684
            |. :||.|.|                 .|..::||          |:|.      ..:|::|.:|
Zfish  1551 SG-DEAFMTQ-----------------NSAKQNEH----------CLKNFDLAEYRQVLSDLSIQ 1587

  Fly  1685 LIKQTTDHPDANSRVNLKNWALLSVLCSVILPSMKAVRKYLIAHLKRCSS 1734
            :.:|.....:|    |::...:.::|.|..:||:..|:.  :.:..|.||
Zfish  1588 IYQQLVKVAEA----NMQPMIVSAMLESESIPSLAGVKP--MGYRNRSSS 1631

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Myo81F	NP_001303492.1	Motor_domain	23..673	CDD:473979	252/687 (37%)
		MyTH4	1091..1194	CDD:459939	17/105 (16%)
		FERM_F1_DdMyo7_like	1198..1299	CDD:340728	13/103 (13%)
		B41	1202..1419	CDD:214604	39/229 (17%)
		PH-like	1415..1523	CDD:473070	14/107 (13%)
		MyTH4	1664..1771	CDD:459939	16/77 (21%)
		FERM_F1_DdMyo7_like	1775..1880	CDD:340728
		B41	1781..1992	CDD:214604
		PH-like	1988..2076	CDD:473070
myo5b	XP_021324561.1	COG5022	7..1371	CDD:227355	397/1494 (27%)
		MYSc_Myo5	83..746	CDD:276831	252/688 (37%)
		Myo5b_CBD	1461..1835	CDD:271261	50/294 (17%)