Protein Alignment Myo81F and MYH3

DIOPT Version :10

• Sequence 1: NP_001303492.1
• Gene: Myo81F / 26067052
• FlyBaseID: FBgn0267431
• Length: 2082
• Species: Drosophila melanogaster
• Sequence 2: NP_002461.2
• Gene: MYH3 / 4621
• HGNCID: 7573
• Length: 1940
• Species: Homo sapiens
• Alignment Length: 2165
• Identity: 500/2165 - (23%)
• Similarity: 844/2165 - (38%)
• Gaps: 535/2165 - (24%)

  Fly    12 DMTEMSEIDEIGINQNLNVRYNSDIIYTYTGSILLAVNPYKTINIFNKQTISEYHTCKLGDLSPH 76
            ||..::.::|..:..||..||.|.:||||:|...:.|||||.:.::|.:.:..|...|..:..||
Human    90 DMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPH 154

  Fly    77 VFAVAEAAYTSIVDDNINQACVISGESGAGKTESTKFILQYLCTIT----------SNVSSWVQQ 131
            :|::::.||..::.|..||:.:|:||||||||.:||.::||..||.          |.:...::.
Human   155 IFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLED 219

  Fly   132 QILEANTILEAFGNAKTTRNDNSSRFGKFMQVCFDDNNCIKGCVILDYLLEQSRITFQSEGERNY 196
            ||:.||.:|||||||||.|||||||||||:::.|.....:....|..||||:||:|||.:.||:|
Human   220 QIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSY 284

  Fly   197 HIMYQLVAQGQKNIGIANTFHLRPAEFYKYLNTSNSEKINMNL-ESKKFDAVTMAFTVLQIPQFV 260
            ||.||:::..:..:.........|   |.|...|..|.:..:: ::::..|...|..:|......
Human   285 HIFYQILSNKKPELIELLLITTNP---YDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEE 346

  Fly   261 IDGVFKVISSILWLGNIEF--------IDVDG-EVTDLTRSDQEAISIISMLLGLSRCDFKKVLL 316
            ..|::|:..:::..||::|        .:.|| ||.|.|          :.|:||:..|..|.|.
Human   347 KSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKT----------AYLMGLNSSDLLKALC 401

  Fly   317 IRQINVRGNITEIPLKIKEALENRH---ALAKALYSKMFTWLVSKINNCTNPGQDDAK-----FL 373
            ..::.| ||  |...|.:...:..|   ||:|::|.|:|.|:|::||.     |.|.|     |:
Human   402 FPRVKV-GN--EYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQ-----QLDTKLPRQHFI 458

  Fly   374 GVLDIFGFENFSHNSFEQLCINYTNEKLHKFFNHYVFALEQSIYEHEGIYFNHVEF-TDNTPCLE 437
            |||||.|||.|.:||.||||||:|||||.:||||::|.|||..|:.|||.:..::| .|...|:|
Human   459 GVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIE 523

  Fly   438 ILEKPPRCVFKLLTEQCHMPQGSDLAFLSNMHTE-------FESHPNYIKGSDRRHWETEFGIKH 495
            ::|| |..:|.:|.|:|..|:.:|.:|.:.::.:       |:. |..:||....|    |.:.|
Human   524 LIEK-PMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQK-PKVVKGRAEAH----FSLIH 582

  Fly   496 YAGLVIYAVGGFVEKNRD-VQQDVMFEYMSYSKDVFIKDLSTY--QDIQFINSSYPRGSSKSKCT 557
            |||.|.|:|.|::|||:| :.:.|:..|...|..:.....:|:  .|.........:....|..|
Human   583 YAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQT 647

  Fly   558 VSDNFRNQLQSLIDVLQNTKPWYVRCIKPNLQKRPNNYNSSLVLDQLKYLGVVDIIRIRREGFPI 622
            ||..||..|..|:..|:.|.|.:||||.||..|.|.....||||.||:..||::.|||.|:|||.
Human   648 VSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPN 712

  Fly   623 HLSHKDFIVRYKCL----IKNKSFEYPQKYLTNILEKNGMPTTEWQIGKSKIFLRNAAYELLETN 683
            .:.:.||..||:.|    |....|...:|....:|....:..|:::.|.:|:|.:......||..
Human   713 RILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEM 777

  Fly   684 RKDTLYKNAVIIQKNWKKYYIQKSFLRNKQAVLRIQHAYRGWRLRIRFMRM---RRSAIVIQSRL 745
            |.|.|.|                       .:.|.|...||:.:|:.|.:|   |.|...||..:
Human   778 RDDRLAK-----------------------LITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNI 819

  Fly   746 RGVF-----------------------AREVAAALREMKRVDEEL----KKRDGIYEQLTSIT-- 781
            |...                       .:|:|....|.::..:||    .||..:.|:|.::.
Human   820 RSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQE 884

  Fly   782 -----------TKTLADCERSIQEELSVLAHISDNIRASAKPLSDKNDSDSN-TAR--------S 826
                       ::.|.|.|....:.:.....:...|:...:...|:.:.::. ||:        |
Human   885 KNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECS 949

  Fly   827 SIKQNTVNLDNMFDFLCESKGAFENALIKEINDNWNNLVKNLDEEI-GLCIHTEDSFDSQKTGKL 890
            .:|::..:|:.....:.:.|.|.||.            ||||.||: ||         .:...||
Human   950 ELKKDIDDLELTLAKVEKEKHATENK------------VKNLTEELSGL---------DETIAKL 993

  Fly   891 FTTKTVRTLPEPTVPPPPPPVSISANLNSEPIYEAINLYKLTTTKEIKKEKYMLLDYKSIKDNSK 955
              |:..:.|.|       .......:|.:|.  :.:|  .|..||  .|.:..:.|.:|..:..|
Human   994 --TREKKALQE-------AHQQALDDLQAEE--DKVN--SLNKTK--SKLEQQVEDLESSLEQEK 1043

  Fly   956 KPSTSSVFVKYQIDERENRRKQRVEKKIHELTLIDIHKDIHIDNSFYNILEFAENYFNTHDLSSD 1020
                     |.::|...|:||...:.|:.:.:::|:..|....:......:|  .|........|
Human  1044 ---------KLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDF--EYCQLQSKVED 1097

  Fly  1021 CNFISTPTQKG--------KSSDKTAKHEMTMFSKSDKIPTSHIHMYDPENVVLSCSMFRELCKY 1077
            ...:....||.        :..::..:.|....:|::|..:.:....:            ||.:.
Human  1098 EQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELE------------ELSER 1150

  Fly  1078 MRG---------ELNAERELQIIQYIVGLGIEREELRDEIFIQCIRQSRNN------PNIDWTDR 1127
            :..         |||.:||.:.::  :...:|...|:.|..:..:|:...:      ..||...|
Human  1151 LEEAGGVTSTQIELNKKREAEFLK--LRRDLEEATLQHEAMVAALRKKHADSVAELGEQIDNLQR 1213

  Fly  1128 IWIILCLSIVAFQPSKLLFRYFVSFIKKSMIELD---GKLRQYSQWCFDNCKSTKVSTRMYPPSS 1189
            :...|       :..|..|:..:..:..||..:.   ..|.:..:...|.....:........|.
Human  1214 VKQKL-------EKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSL 1271

  Fly  1190 VEVAAMR-RLGTIVCRFFFLDARTKAIDVHPTDTAGDAVQKLAEKLNLTTTDGWAIYQNRPDGEE 1253
            .|:...: ||.|                     .||:..::|.||.::.:
Human  1272 SELTTQKSRLQT---------------------EAGELSRQLEEKESIVS--------------- 1300

  Fly  1254 HIKSYDYLYDVISSWEMKQATYAGTKKTTNGSSSGENQFVFKKRLFKSTHELSQDPVEVSMLYSQ 1318
                           ::.::..|.|::|.......|.:...|..|   .|.|.....:..:|..|
Human  1301 ---------------QLSRSKQAFTQQTEELKRQLEEENKAKNAL---AHALQSSRHDCDLLREQ 1347

  Fly  1319 AVHSVVKKDEFPVSEKVALQ--LAGLQAQVALGDPSNQPKPEYYSDINSFLPIRISKTREQQFWI 1381
            .        |.....|..||  |:...::||      |.:.:|.:|       .|.:|.|.:...
Human  1348 Y--------EEEQEGKAELQRALSKANSEVA------QWRTKYETD-------AIQRTEELEEAK 1391

  Fly  1382 PILAQAHRQYGSSRNALTAKVLYLSCVMQYPLYGTTMFKVNYKGYWNLASNIILGIHYDGIMFIN 1446
            ..|||..:.......|:.||...|....|..........|:.:...:||:.:
Human  1392 KKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAAL------------- 1443

  Fly  1447 PEDKSVVFQFKYVDIESIL------LDPSDSFITISLNRSAQLLKTSLYGSSLIESQKCFVFETP 1505
              ||      |..:.:.:|      .:.|.:.:..||..| :.|.|.|:.......:.....||.
Human  1444 --DK------KQRNFDKVLAEWKTKCEESQAELEASLKES-RSLSTELFKLKNAYEEALDQLETV 1499

  Fly  1506 QK------NDIGSLIISYYPALSNWILNNLECSKKYKGTTKED-RLRL--------YQNVVLCRR 1555
            ::      .:|..|  :...|.:...::.||.|:|.....|.| :|.|        ::...:.|.
Human  1500 KRENKNLEQEIADL--TEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRI 1562

  Fly  1556 HL--------VDMNIVKKPHDFGGFLRNTLR--------------------RLSKHR---LEKLR 1589
            .|        :|..|.:|..:.....||..|                    ||.|..   |.::.
Human  1563 QLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIE 1627

  Fly  1590 SEVGSNTQEHGETYKGFSHTFWAFSKQQIPFCISTISDQEEALMIQVFDSILTYSGLGSSGESIS 1654
            .::....::..||.|   |             :.::..|.:...:.:.|::.....|......:.
Human  1628 IQLSHANRQAAETLK---H-------------LRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVE 1676

  Fly  1655 R------AEDEHIRIIQSIMDKCMK--KESLLNELYLQLIKQTTDHPDANSRVNL---KNWALLS 1708
            |      ||.|.:|......::..|  ::.||               |:|.||.|   :|.:|:.
Human  1677 RRANLLQAEVEELRATLEQTERARKLAEQELL---------------DSNERVQLLHTQNTSLIH 1726

  Fly  1709 VLCSVILPSMKAVRKYLIAHLKRCSSDFLSEEGKYARYAENCFFRTQGTRRRQWTPSREEILCTT 1773
                        .:|.|...|.:..|: :.:..:.||.||.
Human  1727 ------------TKKKLETDLMQLQSE-VEDASRDARNAEE------------------------ 1754

  Fly  1774 NRRPCYSKFYFMDGQYYSIEFQPSSTANDVLEIIKKKI--------GLLDNAKGYSIYEVIGNSE 1830
                 .:|....|....:.|.:.....:..||.:||.:        ..||.|:..::.   |..:
Human  1755 -----KAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALK---GGKK 1811

  Fly  1831 RSLSSEEKVCDVMAKWEKYQITSQQGFQKNTTLISQQNQYMFLFKKHLFFD-----NYINLEDIV 1890
            :....|.::.::     ::::..:|  :|||..:....:|....|:..:..     |.:.|:|:|
Human  1812 QIQKLETRIREL-----EFELEGEQ--KKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLV 1869

  Fly  1891 EK-----------------ELLYHQILHNLRSERYPITEMEAIMLTALQSQLERGDCCES-ITDY 1937
            :|                 :...|  |...|..::.:.|.|           ||.|..|| :...
Human  1870 DKLQVKVKSYKRQAEEADEQANAH--LTKFRKAQHELEEAE-----------ERADIAESQVNKL 1921

  Fly  1938 RAVASHCLPPRFVPNIPHEA 1957
            ||........|.|   .||:
Human  1922 RAKTRDFTSSRMV---VHES 1938

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Myo81F	NP_001303492.1	Motor_domain	23..673	CDD:473979	247/692 (36%)
		MyTH4	1091..1194	CDD:459939	16/111 (14%)
		FERM_F1_DdMyo7_like	1198..1299	CDD:340728	12/100 (12%)
		B41	1202..1419	CDD:214604	36/218 (17%)
		PH-like	1415..1523	CDD:473070	19/119 (16%)
		MyTH4	1664..1771	CDD:459939	18/111 (16%)
		FERM_F1_DdMyo7_like	1775..1880	CDD:340728	18/112 (16%)
		B41	1781..1992	CDD:214604	39/208 (19%)
		PH-like	1988..2076	CDD:473070
MYH3	NP_002461.2	Myosin_N	33..77	CDD:460670
		MYSc_Myh3	100..767	CDD:276878	247/693 (36%)
		Actin-binding	656..678		10/21 (48%)
		Actin-binding	758..772		3/13 (23%)
		Myosin_tail_1	847..1924	CDD:460256	227/1339 (17%)