Protein Alignment Myo81F and Myo5a

DIOPT Version :10

• Sequence 1: NP_001303492.1
• Gene: Myo81F / 26067052
• FlyBaseID: FBgn0267431
• Length: 2082
• Species: Drosophila melanogaster
• Sequence 2: NP_001423326.1
• Gene: Myo5a / 25017
• RGDID: 3143
• Length: 1877
• Species: Rattus norvegicus
• Alignment Length: 2076
• Identity: 503/2076 - (24%)
• Similarity: 821/2076 - (39%)
• Gaps: 556/2076 - (26%)

  Fly     8 VGFADMTEMSEIDEIGINQNLNVRY-NSDIIYTYTGSILLAVNPYKTINIFNKQTISEYHTCKLG 71
            ||..|:|.:|.:.|..:..||.||: :|.:||||.|.:|:|:|||:.:.|:.:..|:.|....:|
  Rat    69 VGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMG 133

  Fly    72 DLSPHVFAVAEAAYTSIVDDNINQACVISGESGAGKTESTKFILQYLCTITSNVS-SWVQQQILE 135
            |:.||:|||||.||..:..|..||:.::|||||||||.|.|:.::|..|::.:.| :.|::::|.
  Rat   134 DMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLA 198

  Fly   136 ANTILEAFGNAKTTRNDNSSRFGKFMQVCFDDNNCIKGCVILDYLLEQSRITFQSEGERNYHIMY 200
            :|.|:|:.||||||||||||||||::::.||....|.|..:..||||:||:.||:|.||||||.|
  Rat   199 SNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFY 263

  Fly   201 QLVAQGQ------KNIGIANTFHLRPAEFYKYLNTSNSEKINMNLESKKFDAVTMAFTVLQIPQF 259
            ||.|..:      ..:|.|::||        |.....|..|....::|:......|.|:|.|.:.
  Rat   264 QLCASAKLPEFKMLRLGNADSFH--------YTKQGGSPMIEGVDDAKEMAHTRQACTLLGISES 320

  Fly   260 VIDGVFKVISSILWLGNIEFIDVDGEVTDLTRSDQEAISIISMLLGLSRCDFKKV---LLIRQIN 321
            ...|:|::::.||.|||:.|...|.:...:. ...|.:.|...|:|:   |::::   |..|::.
  Rat   321 YQMGIFRILAGILHLGNVGFASRDSDSCTIP-PKHEPLIIFCDLMGV---DYEEMCHWLCHRKLA 381

  Fly   322 VRGNITEIPLKIKEALENRHALAKALYSKMFTWLVSKINNCTNPGQDDAKFLGVLDIFGFENFSH 386
            ........|:...:|...|.||||.:|:|:|.|:|..:|...:.......|:|||||:|||.|..
  Rat   382 TATETYIKPISKLQATNARDALAKHIYAKLFNWIVGHVNQALHSAVKQHSFIGVLDIYGFETFEI 446

  Fly   387 NSFEQLCINYTNEKLHKFFNHYVFALEQSIYEHEGIYFNHVEFTDNTPCLEILEKPPRCVFKLLT 451
            |||||.||||.||||.:.||.:||.|||..|..|.|.:..::|.||.||:.::|. ...:..||.
  Rat   447 NSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIES-KLGILDLLD 510

  Fly   452 EQCHMPQGSDLAFLSNMHTEFESHPNYIKGSDR-RHWETEFGIKHYAGLVIYAVGGFVEKNRDVQ 515
            |:|.||:|:|..:...:   :.:|.|.....:: |.....|.|||:|..|.|...||:|||:|..
  Rat   511 EECKMPKGTDDTWAQKL---YNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTV 572

  Fly   516 QDVMFEYMSYSKDVFIKDLSTYQDIQFINSSYPRGSS----------------------KSKCTV 558
            .:...:.:..||...:.:|  :||.:...|.....||                      :.|.||
  Rat   573 FEEQIKVLKSSKFKMLPEL--FQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTV 635

  Fly   559 SDNFRNQLQSLIDVLQNTKPWYVRCIKPNLQKRPNNYNSSLVLDQLKYLGVVDIIRIRREGFPIH 623
            ...|||.|..|::.|..|.|.||||||||..|.|..::....:.||:..||::.|||...|||..
  Rat   636 GLQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSR 700

  Fly   624 LSHKDFIVRYKCLIKNKSFEYPQKY-LTNILEKNGMPTTEWQIGKSKIFLRNAAYELLETNRKDT 687
            .::::|..||:.|:|.|.....:|. ..|:|||..:...::|.||:|||.|......||..|.|.
  Rat   701 WTYQEFFSRYRVLMKQKDVLGDRKQTCQNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADK 765

  Fly   688 L------------------------------------------------YKNAVIIQKNWKKYYI 704
            |                                                .|.|..|||.|:.|.:
  Rat   766 LRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVV 830

  Fly   705 QKSFLRNKQAVLRIQHAYRGWRLRIRFMRMRR--SAIVIQSRLRGVFAR---------------- 751
            ::.:...:.|.:.:|...||:..|.|:.::.|  .|::||.|:||..||
  Rat   831 RRKYKIRRAATIVLQSYLRGYLARNRYRKILREHKAVIIQKRVRGWLARTHYKRTMKAIIYLQCC 895

  Fly   752 -EVAAALREMK----------------------------RVDEELKKRDGIYEQLTSI------- 780
             ....|.||:|                            :|||:.|....:.|:||::
  Rat   896 FRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSE 960

  Fly   781 TTKTLADCER----------------SIQEELSVLAHISDNIRASAKPLSDKNDSDSNTARSSIK 829
            |.|...|.||                |:|||::.|....:..|:..|.:.::.|        ..|
  Rat   961 TEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERAD--------KYK 1017

  Fly   830 QNTVNL-DNMFDFLCESKGAFENALIKEINDNWNNLVKNLDEEIGLCIHTEDSFDSQKTGKLFTT 893
            |.|..| .|:.:         ||.|:|:..:..|:|:....:|:                    |
  Rat  1018 QETEQLVSNLKE---------ENTLLKQEKETLNHLMVEQAKEM--------------------T 1053

  Fly   894 KTV-RTLPEPTVPPPPPPVSISANLNSEPIYEAINLYKLTTTKE----IKKEKYMLLDYKSIKDN 953
            :|: |.|.|.|       ..:..:||.|.:.....|.:.:..:|    :|:|..::|       |
  Rat  1054 ETMERKLVEET-------KQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLML-------N 1104

  Fly   954 SKKPSTSSVFVKYQIDERENRRKQRVEKKIHELTLIDIHKDIHIDN-SFYNILEFAENYFNTHDL 1017
            ..||.                         |:.|     ...|..| |.|.   |:..:..|.|:
  Rat  1105 VPKPG-------------------------HKRT-----DSTHSSNESEYT---FSSEFAETEDI 1136

  Fly  1018 SSDCNFISTPTQKGKSSDKTAKHEMTMFSKSDKIPTSHIHMYDPENVVLSCSMFRELCKYMRGEL 1082
            :..   ...||:|....|      |::|.|..|..|.    .:.|..::...:.|:..:.:|.:.
  Rat  1137 APR---TEEPTEKKVPLD------MSLFLKLQKRVTE----LEQEKQLMQDELDRKEEQVLRSKA 1188

  Fly  1083 NAERELQIIQYIVGLGIEREELRDEIFIQCIRQSRNNPNIDWTDRIWIILCLSIVAFQPSKLLFR 1147
            ..|...|    |.|..:|.|.|:        ||...:.|
  Rat  1189 KEEERPQ----IRGAELEYESLK--------RQELESEN-------------------------- 1215

  Fly  1148 YFVSFIKKSMIELDGKLRQYSQWCFDNCKSTKVSTRMYPPSSVEVAAMRRLGTIVCRFFFLDART 1212
                  ||...||: :||:        ..|.|.:..:..|.:.....:....|.|..  .||.|.
  Rat  1216 ------KKLKNELN-ELRK--------ALSEKSAPEVTAPGAPAYRVLMEQLTAVSE--ELDVRK 1263

  Fly  1213 KAI------------DVHPTDTAGDA------VQKLAEKLNLTTTDGWAIYQNRPDGEEHIKSYD 1259
            :.:            .:.|.:|..|:      |||:.:|
  Rat  1264 EEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDK-------------------------- 1302

  Fly  1260 YLYDVISSWEMKQATYAGTKKTTNGSS--------SGENQFVFKKRLFKSTHELSQDPVEVSMLY 1316
                    .|:.|| |.|.|:|...|:        .||...|::.  .|..:.|.:     |.|.
  Rat  1303 --------GEIAQA-YIGLKETNRSSAMDYQELNEDGELWLVYEG--LKQANRLLE-----SQLQ 1351

  Fly  1317 SQAVHSVVKKDEFPVSEKVALQLAGLQ----AQVALGDPSNQPKPEYYSDINSFLPIRISK-TRE 1376
            ||      |:.....:|.:..::..|:    .|..|...:.|..||  :.|.:.|...|:: |.|
  Rat  1352 SQ------KRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPE--ARIEASLQHEITRLTNE 1408

  Fly  1377 QQFWIPILAQAHRQYGSSRNALTAKVLYL------------SCVMQYPLYGTTMFKVNYKGYWNL 1429
            ..::..:.|...::|.|.|.:|..:::.|            ....|..::...:.::......|:
  Rat  1409 NLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENI 1473

  Fly  1430 ASNIILG------------IHYDGIMFINPED-----KSVVFQFKYVDIESILLDPSDSFITISL 1477
            :...|:.            ..:.|::....||     |:::.:.|...:...|:....::|....
  Rat  1474 SPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMC 1538

  Fly  1478 NRSAQLLKTSLYGSSLIESQKCFVFETPQKNDIGSLIISY---YPALSNWILNN---LECSKKYK 1536
            .|.|..|.         :.||.....|...|.|..::...   :..:|.|:.|.   |.|.|:|.
  Rat  1539 VRHADYLN---------DDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYS 1594

  Fly  1537 G--------TTKEDR-------------------LRLYQNVVLCRRHLVDMNIVKKPHDFGGFLR 1574
            |        |::::.                   :::||.:|....:::...||      .|.|.
  Rat  1595 GEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIV------SGMLE 1653

  Fly  1575 N-TLRRLSKHRLEKLRSEVGSNTQEHGETYKGFSHTFWAFSKQQI-----PFCISTISDQEEALM 1633
            : |::.:|..:...||....|...|...|.........:|.....     |..|..:..|    |
  Rat  1654 HETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQ----M 1714

  Fly  1634 IQVFDSILTYSGLGSSGESISRAEDEHIRIIQSIMDKCMKKESLLNE-------------LYLQL 1685
            ..:..:| |.:.|....:..|.::...||...|.:::.::.::|:|.             ..||:
  Rat  1715 FYIVGAI-TLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQV 1778

  Fly  1686 IKQTTDHPDANSRVNLKNWALLSVLCSVILPS--MKAVRKYLIAHLKRCSSDFLSEEGKYARYAE 1748
            .|:|.|..:|           :..:|:.:..:  :|.:..|             :...::.....
  Rat  1779 KKKTDDDAEA-----------ICSMCNALTTAQIVKVLNLY-------------TPVNEFEERVS 1819

  Fly  1749 NCFFRTQGTRRRQWTPSREEILCTTNRRPCYSKFYFMDGQY---YSIEFQPSSTANDVLEI 1806
            ..|.||...|.|....|.:               ..||.::   .:..|.|||.|.:.::|
  Rat  1820 VSFIRTIQVRLRDRKDSPQ---------------LLMDAKHIFPVTFPFNPSSLALETIQI 1865

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Myo81F	NP_001303492.1	Motor_domain	23..673	CDD:473979	250/684 (37%)
		MyTH4	1091..1194	CDD:459939	17/102 (17%)
		FERM_F1_DdMyo7_like	1198..1299	CDD:340728	23/126 (18%)
		B41	1202..1419	CDD:214604	48/259 (19%)
		PH-like	1415..1523	CDD:473070	17/127 (13%)
		MyTH4	1664..1771	CDD:459939	18/121 (15%)
		FERM_F1_DdMyo7_like	1775..1880	CDD:340728	8/35 (23%)
		B41	1781..1992	CDD:214604	8/29 (28%)
		PH-like	1988..2076	CDD:473070
Myo5a	NP_001423326.1	COG5022	7..1445	CDD:227355	425/1595 (27%)
		MYSc_Myo5	83..751	CDD:276831	250/685 (36%)
		Myo5a_CBD	1503..1877	CDD:271262	74/422 (18%)