Protein Alignment Pzl and Piezo2

DIOPT Version :9

• Sequence 1: NP_001303493.1
• Gene: Pzl / 26067051
• FlyBaseID: FBgn0267430
• Length: 2326
• Species: Drosophila melanogaster
• Sequence 2: XP_006254901.1
• Gene: Piezo2 / 307380
• RGDID: 1582973
• Length: 2822
• Species: Rattus norvegicus
• Alignment Length: 2943
• Identity: 711/2943 - (24%)
• Similarity: 1201/2943 - (40%)
• Gaps: 760/2943 - (25%)

  Fly     2 AEPLLFIITRIFLPLTLILVSLVRPVGLSYFYLLTFFLSPWLLQSNSLKQRTFLNVFIIFLCVLS 66
            :|.:..:|.|:.||:.|.:....|..|||:.||:...|.|...:......:......:..||:.|
  Rat     3 SEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKATMQGHTGRLLQSLCITS 67

  Fly    67 TFGIVGHIIFNLINLFFVDLSSK-------NSFSFILRQFGFLYFKGLSFVSIAHWILPDIIVLL 124
            ...::.||||: |.|..::...:       :::....||.||...||....:.....:|||.:.:
  Rat    68 LSFLLLHIIFH-ITLASLEAQHRIAPAYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFI 131

  Fly   125 NTIVFFIFSEIKYKR---------------------------EKRVIQNDDDVFYKKSKELKGAL 162
            .::..::......|:                           |..:.:.|.|.......||:.:.
  Rat   132 ASLTIWLVCRTIVKKPDTEEVAQFNSECENEELAGGEKMDSEEALIYEEDLDGEEGMEGELEEST 196

  Fly   163 KVAEINRVLIIV-----YIRSVLKTS-LIFSLIVLYFAATLRPSLPGSLYFCMFIIAGSYWALFR 221
            |:....|:..:.     :|.:::.|: .:...|:|..:..:.|||..::||.:|:...::|:..|
  Rat   197 KLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSAVYFFVFLGLCTWWSWCR 261

  Fly   222 Q-RKMYYSVIFMIVALLV--HITCIFAYQLPVLQNTINCDKLWTRLMGMEVLIRLNKDNRNGKIL 283
            . ..:.:..:.:::|:..  |:..::.||....|..:..:..:.||.|::.:|:.:..: ..||:
  Rat   262 TFDPLLFGCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSS-TWKII 325

  Fly   284 ELNPKLNLDSYLSPIALMLAYF--ATTL----------------------------------SLI 312
             :||.|:...:.:||.|::.|:  ||.:                                  ||.
  Rat   326 -VNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQEEMSKEEEGALDGCSNQNTAERRRSLW 389

  Fly   313 NRSQY----------------------------EISFNTIRMNL----GPKSNSIYDNIQ----- 340
            ..:||                            .:.::||..:|    ||....:|...|
  Rat   390 YATQYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPIENGPAKTDLYTTPQYRWEP 454

  Fly   341 --PSNEENEEISIFTEPSMLEQF--------FYIVYELSSFVYKNSYILLNIIMMIWSIVYHSWL 395
              .|:|:.||.....|.|..|..        .:.:..:..|:.|.|||...|.||.|||.|||||
  Rat   455 SEESSEKKEEEEDKREDSEGEGSQEEKRSVRIHAMVAVFQFIMKQSYICALIAMMAWSITYHSWL 519

  Fly   396 TFVLLIWANILWMIPNQRRSMMRSSLFVVFYSQILLLAQYIYGMNLYE-SELPTKVTSCGLNLEQ 459
            |||||||:..||||.|:|:..|.||.|:|.|:.:||:.|||:...|.| .::|
  Rat   520 TFVLLIWSCTLWMIRNRRKYAMISSPFMVVYANLLLVLQYIWSFELPEIKKVP------------ 572

  Fly   460 IGFVHPQSKDSLPWIPLTVKTVFLFVFWVTSRQYFREISLDRQQSDLLQQIFATHRSRSEHLLSK 524
             ||:..:....     |..|.:|...||:..||:..|....:::..||.::    :..|:.|..|
  Rat   573 -GFLEKKEPGE-----LASKILFTITFWLLLRQHLTEQKALQEKEALLSEV----KIGSQELDEK 627

  Fly   525 KSQTPQIFIY-----------------------------------ILKSTSHFITRIWMWLLIFL 554
            ..:...|.:.                                   |:|...:.:..:::...|::
  Rat   628 DEELQDIQVEGEPIEKEEEEEEIKEDRHEVKKEEEEEEEDDDDQDIMKVLGNLVVALFIKYWIYV 692

  Fly   555 ---IFLCAMIDRTMTGIRICYMSLFLLFLMVFQMSLQLWIRFLYGYWIFLIFYAMTILTAIYTYQ 616
               :|.....:..:...:|.||.|||..:.::|:..:.|.:.|..:|:.::.|.|.:|..|||||
  Rat   693 CGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQ 757

  Fly   617 FDHFDYYWEKFLKVHSTLQCDIGLRRYQTKDLFLHLLIPTLTVIFTVVQLHYFHRRFVE------ 675
            |::|...|:....:......|:||:::...:||..:.|||..::..::.|||||.||:|
  Rat   758 FENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKS 822

  Fly   676 ---------------------SVRRPRSTEG-----------------QNFHLAPNAETRA---- 698
                                 .:.|....||                 :...||...|.|.
  Rat   823 IPSKEDNTIYSHAKVNGRVYLIINRLAHPEGSLPDLAIMNMTASLDKPERQKLAEPGEERTEECA 887

  Fly   699 --------------------------SQKTLDIHRQSWKVFFDAGYENVRAKGRQLFNPGKIVAW 737
                                      .:::.|: |..|.:..|. ...:..|..:.|:..::..|
  Rat   888 KKAEKGEAGKDSDESEEEEGEEEESEEEESSDL-RNKWHLVIDR-LTVLFLKFLEYFHKLQVFMW 950

  Fly   738 KFLEMHMIKTVTLASFYCAISEICFLNLFLVILSLLGICGNRFLRRVIFRAVTFWISLLVLMKMI 802
            ..||:|:||.|:....:..:.|:...| ::.::|.........||||.....|.|..::::.||:
  Rat   951 WILELHIIKIVSSYIIWVTVKEVSLFN-YVFLISWAFALPYAKLRRVASSVCTVWTCVIIVCKML 1014

  Fly   803 YQIKYLDQSHYNFKCG-------NVTVNF-------------AEWMGLRKTGTVFGVHLRYISSY 847
            ||::.:...:::..|.       |:.:|.             .||:||||:..:. |:||  ::.
  Rat  1015 YQLQTIKPENFSVNCSLPNENQTNIPLNELNKSLLYSAPVDPTEWVGLRKSSPLL-VYLR--NNL 1076

  Fly   848 IIYMILTSLHAVVKLRDHLIRYSTHKQKDTKLLFPDITRQDAERDFPGLLKYVLNYGYFKFGFEI 912
            ::..||.....|.:.:::....:......:|.:|.||||...:.......||.:||.::|||.|.
  Rat  1077 LMLAILAFEVTVYRHQEYYRGRNNLTAPVSKTIFHDITRLHLDDGLINCAKYFVNYFFYKFGLET 1141

  Fly   913 TMIGLMSTIAHRRDLFALTYLIWLVLLLCLSRFRC-ARIWEMLQLYFVLSIFVQYVYLLNFPPNL 976
            ..:..::.|..|.|.:|:.:..||:.:|...|.:. |.:|.....:....|..||...:..||..
  Rat  1142 CFLMSVNVIGQRMDFYAMIHACWLIGVLYRRRRKAIAEVWPKYCCFLACIITFQYFVCIGIPPAP 1206

  Fly   977 CNVISYNSLWSISGSK------------NYTNRSN---IMFDYIVLLLISRQRKSFRVEMRHIND 1026
            |.    :..|...|:.            ::..|.|   :::|:::||..|.||:.|..|    |.
  Rat  1207 CR----DYPWRFKGANFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDE----NK 1263

  Fly  1027 LSYR--GGVNTNVVHNI--AKLGHVYFENPTHDFCTYVRNYADVLKTAIFFSFFWITLAIVFLGG 1087
            .:.|  .|.|..:..|:  |....   .||..|| .:.|:|.|:.|..||...||..|.|:|:.|
  Rat  1264 AAVRIMAGDNVEICMNLDAASFSQ---HNPVPDF-IHCRSYLDMSKVIIFSYLFWFVLTIIFITG 1324

  Fly  1088 VCSTDILSLGYIIFALIFLLQGSEIYLQNIHFIICRWNCLIAFNIFNIIVK----------VAII 1142
            .....|..:||::....|||.|.::.|:.|..|:..|:.|||:|:|.|.:|          :..:
  Rat  1325 TTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGAL 1389

  Fly  1143 VLEGTINLEHKSILSIVFNVMHQTAPCENIMDGKFFEINEISEKKYCYGPSDMIFKNTLVWHAII 1207
            |......::..|:...|                |.:::.|  :...|..||.   :..::|.:|.
  Rat  1390 VRNSCWLIQAFSLACTV----------------KGYKMPE--DDSRCKLPSG---EAGIIWDSIC 1433

  Fly  1208 FSFVVFQYRIFGSYYFCHVIMDTQANTILASRGAAIIENLHYKQIYDRRKNEQSVLERVKMKMER 1272
            |:|::.|.|:|.||||.||:.|.:|:.|||||||.:.:....|.:..|.:.|:..::::|.:|:|
  Rat  1434 FAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDR 1498

  Fly  1273 IRITNRRHQKQATGLDFQPHKPPSSIIHVHYRAEEKSKLKTLNNEFLTTIKQAERDTYFSRSLKP 1337
            |:   .|.||...|.:        .::.:...:.|...::.::.|    ..:.|.|...::..|.
  Rat  1499 IK---ARQQKYKKGKE--------RMLSLTQESGEGQDIQKVSEE----DDEREADKQKAKGKKK 1548

  Fly  1338 R-RRDLSLHPHAVRTGDYYMFNELDDDDDLDVYEEYDFLEKED---------------------- 1379
            : .|....|...||:||||:| |.|.::     ||.:.|:|||
  Rat  1549 QWWRPWVDHASMVRSGDYYLF-ETDSEE-----EEEEELKKEDEEPPRKSAFQFVYQAWITDPKT 1607

  Fly  1380 -VRARQMRQSLRSRASNYNRSKSFLN-----DENSDTDTKKLETHPVIILTSELIVL-------- 1430
             :|.|:..:...:|.....|.|....     ::..|...||....|..|:.....:|
  Rat  1608 ALRQRRKEKKKMAREEQKGRRKGSGEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFL 1672

  Fly  1431 -----LTFRLNRLSKN-------FRFVHKMLSAEKKK----------------LQNIS------- 1460
                 .|..||.:|:.       .|....||:.|.||                :.|:|
  Rat  1673 ATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHMMNLSRESGLDT 1737

  Fly  1461 ----------------------------------------------------------------- 1460
                                                                             
  Rat  1738 IDEHSGAGSRAQAAHRMDSLDSHDSISSCYTEATLLISRQSTLDDLDGQDPVPKTSERARPRLRK 1802

  Fly  1461 --------------------------IMNRLGLSNT---------AAMFKFLNKSLNNKVIK--- 1487
                                      :.:|.|.:.|         ..:.:.|...|::...|
  Rat  1803 VFSMDMSSSSADSGSVASSEPTQCTMLYSRQGTTETIEEVEAEAEEEVVESLEPELHDAEEKEYA 1867

  Fly  1488 ---EAG--------------------------SY----------YVSQD------------NDFT 1501
               |||                          ||          :.|||            :|..
  Rat  1868 ADYEAGVEEVSLTPDEELPQFSTDDGDVEAPPSYSKAVSFEHLSFASQDDSGGKNHMVVSPDDSR 1932

  Fly  1502 T--------------------------------------LDHNDFVQMLIALWYALIANTETICY 1528
            |                                      :|...|:.:..|::..|:|.:|.:||
  Rat  1933 TDKLESSILPPLTHELTASDLLMSKMFRDDELEESEKFYVDQPRFLLLFYAMYNTLVARSEMVCY 1997

  Fly  1529 LVVFLNQAANSSVISLPMPFLVLCWGALTLPRPSKTFWVTLITYNLTMIFVK------------- 1580
            .|:.||...::|:|:|.:|.|:..|..|::||||:.||:..|.|....|.||
  Rat  1998 FVIILNHMISASIITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKD 2062

  Fly  1581 -SIMHQRVLLDQQLVSRSNSLSFELIMKRGRAVYDLLLLVVLFWHQYMLKKQGIW---TIQRTNS 1641
             .|...|......::.        :..|.|..:|||:.|:.||:|:.:||..|:|   .|..:|:
  Rat  2063 LEIYKDRPYFPPNIIG--------VEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDIVDSNT 2119

  Fly  1642 ELLLLEKKRNHYKRSFSKERPENIRKLLREEGGGEQVKAV---------------RPNAIEFKRE 1691
                 :|:.:..:.|..:.|          .|..|.:|::               :|.||..||.
  Rat  2120 -----DKEGSDDELSLGQGR----------RGSSESLKSINLAASVESVHVTFPEQPAAIRRKRS 2169

  Fly  1692 MDDILL---------------PALENK--------YVYQNIESEYYRKAMQGLANKGGLLS---- 1729
            .....:               .:..|.        .:.|..:.|.|.:.:|....|....:
  Rat  2170 CSSSQISPRSSFSSNRSKRGSTSTRNSSQKGSSVLSIKQKSKRELYMEKLQEHLIKAKAFTIKKT 2234

  Fly  1730 -----GFRKFFLALRHKSRLS-TDVYTLLFLCDFISFFILLFGFPKFVYRYKYSTSV-LETYIQG 1787
                 ..|:||..|.|....: ||||.|:||.|.:.|.|::|||..|   .|:|.:. :.:.:..
  Rat  2235 LQIYVPIRQFFYDLIHPDYSAVTDVYVLMFLADTVDFIIIVFGFWAF---GKHSAAADITSSLSE 2296

  Fly  1788 NKVPFTFLLMLIVQFITIVTERAIYLRKALIYKIFFHFISVLGIHIWMFFLVPYITSHSFGET-A 1851
            ::||..||:|:::||.|:|.:||:||||.::.|:.|..|.|.|||.||||::|.:|...|.:. .
  Rat  2297 DQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPSVTERKFSQNLV 2361

  Fly  1852 PVLFYLIKCLHMLLSAYQIRCGYPKRILGNVFTKGYSLANYIAFKIYMEIPFLYILRTMLDWVCI 1916
            ..|:|.:||::..|||||||||||.|:|||..||.|:..|...|:.:..:|||..||.::|||..
  Rat  2362 AQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWT 2426

  Fly  1917 DTTLTVMEWIKMEDIFQSVFIVRCYRQMDTDFPVLRGEPKALYSKLLIGGTIILILIALIWSPLF 1981
            ||||::..||.:|||:..:||::|:|:.:..:|..||:.|....|..:||.||::||.::|.||.
  Rat  2427 DTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKAVKYGMGGMIIVLLICIVWFPLL 2491

  Fly  1982 LFALVGTV-GKPNIPQKADITVKINHYEPIY-VSQSNSDILQFSNSDFQKLTNHIILDNYASDSM 2044
            ..:|:.:| |..|.|....:|:.:..|:||: :|...|.:.....:.:.:.......::.|...:
  Rat  2492 FMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDGTKYNEFIKSFGPNSGAMQFL 2556

  Fly  2045 MLYDAVDVTAIKFYENSISLWNMPPPDKERLLHDLSN-GETLDIHLTLTLKCNLT----PEAVIY 2104
            ..|:..|:|..:...||.|||.:.||.|::::.:|:: .....:..:.:::.|:|    .|....
  Rat  2557 ENYERQDITVAELEGNSNSLWTISPPSKQKMIQELTDPNSCFSVVFSWSIQRNMTLGAKAEIATD 2621

  Fly  2105 ETTYTLTENKVHTRDKLIRLMSNN---FSNEKVIIPNILPKFITVQRQQANAKFIKDY--DGKF- 2163
            :.::.||:   .||..:.::::.|   .||..|.|..|.|.::... ..:|:|.||..  :..|
  Rat  2622 KLSFPLTD---ATRSSIAKMIAGNDTESSNTPVTIEKIYPYYVKAP-SDSNSKPIKQLLSENNFM 2682

  Fly  2164 NRPIILTK--IGKSQVYWWEMNDYCNDRFYTEILSALPLSNCENGVVLYVFNDKSFPSAMSFLEK 2226
            |..|||.:  :.||...||.:| ....|.:.:...||.         |.|||||..|.::.||..
  Rat  2683 NITIILFRDNVTKSNSEWWVLN-LTGSRIFNQGSQALE---------LVVFNDKVSPPSLGFLAG 2737

  Fly  2227 TGIIGLYTTFVYVVSRIIRSLIANNHRRIMFEDLPYVDRVLKLCTDIYLVRETQEYRLEEDLYGK 2291
            .||:|||.:.|.|:.:.:|...:.....||||:||.|||:|||||||:|||||.|..||||||.|
  Rat  2738 YGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAK 2802

  Fly  2292 LIFLYRSPETLIKWTRFK 2309
            ||||||||||:|||||.|
  Rat  2803 LIFLYRSPETMIKWTREK 2820

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pzl	NP_001303493.1	PIEZO	<1201..1388	CDD:292539	57/210 (27%)
		Piezo_RRas_bdg	1901..2308	CDD:288978	150/421 (36%)
Piezo2	XP_006254901.1	PIEZO	1381..1612	CDD:292539	64/272 (24%)
		Piezo_RRas_bdg	2410..2819	CDD:288978	150/422 (36%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	407	1.000	Domainoid score	I681
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1062	1.000	Inparanoid score	I236
OMA	1	1.010	-	-		QHG46055
OrthoDB	1	1.010	-	-		D13738at2759
OrthoFinder	1	1.000	-	-		FOG0001637
OrthoInspector	1	1.000	-	-		mtm9141
orthoMCL	1	0.900	-	-		OOG6_102664
Panther	1	1.100	-	-	O	PTHR13167
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1528
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	10	10.070