Protein Alignment Pzl and piezo2

DIOPT Version :9

• Sequence 1: NP_001303493.1
• Gene: Pzl / 26067051
• FlyBaseID: FBgn0267430
• Length: 2326
• Species: Drosophila melanogaster
• Sequence 2: XP_012820813.2
• Gene: piezo2 / 100492729
• XenbaseID: XB-GENE-1005347
• Length: 2822
• Species: Xenopus tropicalis
• Alignment Length: 2961
• Identity: 706/2961 - (23%)
• Similarity: 1186/2961 - (40%)
• Gaps: 794/2961 - (26%)

  Fly     2 AEPLLFIITRIFLPLTLILVSLVRPVGLSYFYLLTFFLSPWLLQSNSLKQRTFLNVFIIFLCVLS 66
            :|.:..:|.|..|||.|::..:.|..|:|:.||:...|.|...:...:..:......:..|||.|
 Frog     3 SEVVCGLIFRFLLPLCLVVACMFRYNGISFVYLIYLLLIPLFSEPTKITMQGHTGRLLKSLCVSS 67

  Fly    67 TFGIVGHIIFNLINLFFVDLSSK-------NSFSFILRQFGFLYFKGLSFVSIAHWILPDI---I 121
            ...::.||||. |.::.::....       :::...:||.||...||....:......|||   |
 Frog    68 LSFLLLHIIFQ-ITVYSLEARKHIEPHFNCSTWEKAIRQIGFESVKGADAGNGIRVFAPDIGMFI 131

  Fly   122 VLLNTIVF-------------------FIFSEIKYKREKRVIQNDDDVFYK-----KSKELKGAL 162
            ..|.|::.                   |:..|:   .|:..:..|:.:.|:     :..|::.|.
 Frog   132 ASLATLLVCRKLVQKTVTEEDAQYNPQFVNEEM---AEEGKVDLDEALMYEEDFDGEEDEIEAAE 193

  Fly   163 KVAEINRVLIIVYIRSVLKTSL---------IFSLIVLYFAATLRPSLPGSLYFCMFIIAGSYWA 218
            :..::..:..|....||||..:         :...|:|.....:.|||..::||.:|:...::|:
 Frog   194 ESTKLKILRRIASFASVLKEFIGNIITTAGKVVITILLGLTGMMLPSLTSAVYFFVFLGLCTWWS 258

  Fly   219 LFRQRKMYYSVIFMIVALLV------HITCIFAYQLPVLQNTINCDKLWTRLMGMEVLIRLNKDN 277
            ..|   ::..:||..:.:|:      |:..::.|||......|..:..:.||.|:..|::  .|.
 Frog   259 CCR---VFDPLIFSCLCVLMAIFSAGHLIGLYLYQLQFFHEIIPPNDFYARLFGVTSLVQ--TDC 318

  Fly   278 RNGKILELNPKLNLDSYLSPIALMLAYF--ATTLSL----------------------------- 311
            .|......|..|..:.|.:|:.|::.|:  ||.:.|
 Frog   319 CNTWRFFANNSLPWNQYTNPVMLLVLYYTLATLIRLWLQEPINVEEEKEEDCSPAALTAERRRSL 383

  Fly   312 ---------------INRSQYE-------------ISFNTIRMNL----GPKSNSIYDNIQ---- 340
                           :.:..|:             :.:.::..:|    ||....:|...|
 Frog   384 WYSRHYTTDERQLLSMTQDDYKPSDVLLVTVNGTPVDYCSLNSSLPIENGPTKIDMYSTPQYKWD 448

  Fly   341 -------------PSNEENEEISIFTEPSMLEQFFYIVYELSSFVYKNSYILLNIIMMIWSIVYH 392
                         ...|.|||::...|.:.:    :::.....|:.|.|||...|.||.|||.||
 Frog   449 HEDETSEKKEEEEQEEEPNEEVAEEKENTKV----HVMVVAFQFIMKQSYICALISMMAWSITYH 509

  Fly   393 SWLTFVLLIWANILWMIPNQRRSMMRSSLFVVFYSQILLLAQYIYGMNLYESELPTKVTSCGLNL 457
            ||||||||||:..|||:.|:|:..|.||.|:|.|..:||..|||:.:.|...|||
 Frog   510 SWLTFVLLIWSCALWMVRNRRKYAMLSSPFMVMYGNLLLTLQYIWSIALQSDELP---------- 564

  Fly   458 EQIGFVHPQSKDSLPWIPLTVKTVFLFVFWVTSRQYFREISLDRQQSDLLQQIFATHRSRSEHLL 522
            |.:|.:..:..:.     |..|.:|...||:..||:..|....:|:...|.:|......:...:.
 Frog   565 EVMGLLERKKPEE-----LVSKIIFTVTFWLLLRQHLTEQKALKQKELALSEIKVGSEDKEGEVQ 624

  Fly   523 SKKSQTP---QIFIYILKSTSHFITRIWMWLLIFLIFLCAMIDRTMTGIRICYMSLFLLFLMVFQ 584
            .::.:..   .|...:.|.......:.|:::...:.|..: .:..:...:|.||.|||..:.::|
 Frog   625 EEEEEVEDENDIMKVLGKLVQAMFVKYWIYVCGGMFFFVS-FEGKIVMYKIIYMMLFLFCVALYQ 688

  Fly   585 MSLQLWIRFLYGYWIFLIFYAMTILTAIYTYQFDHFDYYWEKFLKVHSTLQCDIGLRRYQTKDLF 649
            :..:.|.|.|..:|:.::.|.|.:|..||||||..|...|.....:......|:||..:...|||
 Frog   689 VHYEWWRRILKYFWMSVVVYTMLVLVFIYTYQFKSFPGLWMNMTGLDKEKLADLGLETFNLTDLF 753

  Fly   650 LHLLIPTLTVIFTVVQLHYFHRRFVESVRRPRSTEGQNFHLAPNAETRA---------------- 698
            ..:.|||..::..::.|||||.||::.......|..|:..:..:|:...
 Frog   754 TRIFIPTSFLLVCILHLHYFHDRFLQLTDLKAVTSKQDSTIYSHAKVNGRVYLIINSIKQKIPIQ 818

  Fly   699 --------------------------SQKTLDIH------------------------------- 706
                                      .:|.|:|:
 Frog   819 QNELVHPEGSLPDITMMNLTTESEMLEEKRLEIYDEKKTHDQDSDDEWDKGDSKEPGHSDDEAED 883

  Fly   707 ------------RQSWKVFFDAGYENVRAKGRQLFNPGKIVAWKFLEMHMIKTVTLASFYCAISE 759
                        |..|.:..|. ...:..|..:.|:..::..|..||:||||.|:....:..|.|
 Frog   884 EESEEEETTTESRNKWHLVIDR-LTVLFLKFLEYFHKFQVFVWWLLELHMIKIVSSYIIWLTIKE 947

  Fly   760 ICFLNLFLVILSLLGICGNRFLRRVIFRAVTFWISLLVLMKMIYQIKYLDQSHYNFKC------- 817
            :..||....|.....:..::| |.:.....|.|..::::.||:||:..:|.:.::..|
 Frog   948 VSLLNYVFFIAWAFALPYSQF-RPLASSVCTVWTCVIIICKMLYQLASIDPATFSSNCLMPQLNE 1011

  Fly   818 --------------GNVTVNFAEWMGLRKTGTVFGVHLRYISSYIIYMILTSLHAVV-------K 861
                          .|.:|:.:.|:||:|:..:    |.|:.:.::.:.:.:....:       :
 Frog  1012 TTARNQTELNSSVLYNSSVDPSNWVGLKKSTPL----LAYLRNNLLMLAMLAFEVTIYRHQEYYR 1072

  Fly   862 LRDHLIRYSTHKQKDTKLLFPDITRQDAERDFPGLLKYVLNYGYFKFGFEITMIGLMSTIAHRRD 926
            ||::|...:      ||.:|.||||...:.......||.:||.::|||.|...:..::.|..|.|
 Frog  1073 LRNNLTTPA------TKTIFHDITRLHLDDGLINSAKYFVNYFFYKFGLETCFLLSVNVIGQRMD 1131

  Fly   927 LFALTYLIWLVLLLCLSRFRC-ARIWEMLQLYFVLSIFVQYVYLLNFPPNLCNVISYNSLWSISG 990
            .||:.:.:||..:|...|.:. |..|.....:....:..||...:..||..|.    :..|...|
 Frog  1132 FFAMVHALWLFGVLYRRRRKAIAETWHRYCCFLACIMAFQYFICIGIPPAACK----DYPWRQPG 1192

  Fly   991 SKNYTNRSNI------------------MFDYIVLLLISRQRKSFRVEMRHINDLSYR--GGVNT 1035
            : |:  .|||                  ::|:::||..|.||:.|..|    |..:.|  .|.|.
 Frog  1193 A-NF--NSNIIKWLYFPDFIVRPSPLFLVYDFLLLLCASLQREVFEEE----NKAAVRIMAGDNV 1250

  Fly  1036 NVVHNI--AKLGHVYFENPTHDFCTYVRNYADVLKTAIFFSFFWITLAIVFLGGVCSTDILSLGY 1098
            .:..|:  |....   .||..|| .|.|:|.|:.|..:|...||..|.|:|:.|.....|..:||
 Frog  1251 EICMNLDAASFSQ---HNPVPDF-IYCRSYLDMGKVIMFSYLFWFVLTIIFITGTTRISIFCMGY 1311

  Fly  1099 IIFALIFLLQGSEIYLQNIHFIICRWNCLIAFNIFNIIVKVAIIVLEGTINLEHKSILSIVFNVM 1163
            ::....|||.|.::.|:.|..|:..|:.|||:|:|.|.:|..:.:  |........|....:.:.
 Frog  1312 LVACFYFLLFGGDLLLKPIRIILRYWDWLIAYNVFVITMKNVLSI--GACGYIEYLIKDSCWLIQ 1374

  Fly  1164 HQTAPCENIMDGKFFEINEISEKKYCYGPSDMIFKNTLVWHAIIFSFVVFQYRIFGSYYFCHVIM 1228
            ..:..|  .:.|.|...|...    |..||.   :..::|.:|.|:|::.|.|:|.||||.||:.
 Frog  1375 AFSLSC--TVKGYFIPQNTPD----CQLPSG---EAGIIWDSICFAFLLLQRRVFMSYYFLHVVA 1430

  Fly  1229 DTQANTILASRGAAIIENLHYKQIYDRRKNEQSVLERVKMKMERIRITNRRHQKQATGLDFQPHK 1293
            |.:::.|||||||.:.:....|.:..|.:.|:..::::|.:|:.|:....:::|
 Frog  1431 DIKSSQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDHIKARQEKYKK----------- 1484

  Fly  1294 PPSSIIHVHYRAEEKSKLKTLNNEFLTTIKQAERDTYFSRSLKPRRRDLSL--------HPHAVR 1350
                       .:||....|.:.|.....|..:.|....::.|.:.:....        |...|:
 Frog  1485 -----------GKEKMLSLTQDGEGQEVQKLDDDDEEGDKADKEKAKGKKKQWWRPWVDHASMVK 1538

  Fly  1351 TGDYYMF---NELDDDDDLDVYE---------------------------------EY-----DF 1374
            :||||:|   :|.:|:||....|                                 :|     .|
 Frog  1539 SGDYYLFETDSEEEDEDDKKEEEPRRKSAFQRAIGKFASAIIALPKSVIKLPKTILQYLIRAAKF 1603

  Fly  1375 L-----------------EKEDVRARQMRQ------------SLRSRASNYNRSKSFLNDENSDT 1410
            |                 |.::.:||:||:            ..:.......:.||    :..|.
 Frog  1604 LYQAWITDPKTALKQRSMEAKEEKAREMRRKGIAEGDIIVECGDKEEEKEQGKKKS----DGPDN 1664

  Fly  1411 DTKK----LETHPVIILTSELIVLLTFRLNRLSKNFRFVHKMLSAEKKKL--------------- 1456
            ..|:    |:...|:.|.:  |...|..||.:|:....:.|:||.|:..|
 Frog  1665 IFKRIFNILKFTWVLFLAT--IDSFTAWLNSISREHIDISKVLSIERCMLTREIKKGNVPTRESI 1727

  Fly  1457 ----QN---ISIMNRLGLSN--------------------------------------TAAMFKF 1476
                ||   :|:....||..                                      |.|...|
 Frog  1728 HMYYQNQIMMSLSRESGLDRIDDQPGSSSSHHSHDKMASKRMDSADSAVSHDSISSCCTEATMLF 1792

  Fly  1477 LNKSL-----------------------------------------------------NNKVIKE 1488
            ..:|.                                                     ..:.|:|
 Frog  1793 SRQSTLDDLDGPDSVPKTSERARPRLRKMYSMDMSSSSADSIASSEPTQCTMVYSRQGTTETIEE 1857

  Fly  1489 ------------------------------------------------------AGSY----YVS 1495
                                                                  |||:    :||
 Frog  1858 VEDEEEEESKSPLSKIEAEEEEYDMDLEGTSYTPDTEFPLYATTEGDLPPSYSKAGSFEQLTFVS 1922

  Fly  1496 QDN-----------------------------------------DFTTLDHNDF-------VQML 1512
            ||:                                         |....|...|       :|::
 Frog  1923 QDSADNLMVLSPDESRTDKLHDHILPPLTHELTASELLLNKMFYDEELEDSEKFYTTQPRPLQLI 1987

  Fly  1513 IALWYALIANTETICYLVVFLNQAANSSVISLPMPFLVLCWGALTLPRPSKTFWVTLITYNLTMI 1577
            .||:..|:|.:|.:||.|:.||...::|:|:|.:|.|:..|..|::|||||.||:|.|.|....|
 Frog  1988 YALYNTLVARSEMVCYFVIILNHMISASMITLVLPILIFLWAMLSVPRPSKRFWMTAIVYTEVTI 2052

  Fly  1578 FVKSIMHQRVLLDQQLVSRS--------NSLSFELIMKRGRAVYDLLLLVVLFWHQYMLKKQGIW 1634
            .:|...........:.|..:        |.:..|  .|.|...|||:.|:.||:|:.:||..|:|
 Frog  2053 VIKYFFQFGFFPWNKNVDYTKDKPYFPPNIMGIE--KKEGYVHYDLVQLLALFFHRSILKCHGLW 2115

  Fly  1635 -----------------------------------------------------TIQRTNSELLLL 1646
                                                                 .|:|.::.....
 Frog  2116 DEDDVTEKATDKDDTDGEISEASGRRDSVGSLKSVNLAASVESVHVHFPEEKTAIRRKSTSSGSQ 2180

  Fly  1647 EKKRNHYKRSFSKERPENIRKLLREEGGGEQVKAVRPNAIEFKREMDDILLPALENKYVYQNIES 1711
            ..:|:.|....||....:.|...::   |..:.:::   ::.::|   ::|..|..    |.|::
 Frog  2181 ISRRSSYSSHRSKRGSTSTRNSSQK---GSSIPSIK---LKSRKE---LILEKLRE----QLIKA 2232

  Fly  1712 EYY--RKAMQGLANKGGLLSGFRKFFLALRHKSRLS-TDVYTLLFLCDFISFFILLFGFPKFVYR 1773
            :.:  :..:|       :....|:||..|.|....: ||||.|:||.|.:.|.|::|||..|
 Frog  2233 KAFTIKTILQ-------IYVPIRQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAF--- 2287

  Fly  1774 YKYSTSV-LETYIQGNKVPFTFLLMLIVQFITIVTERAIYLRKALIYKIFFHFISVLGIHIWMFF 1837
            .|:|.:. :.:.:..::||..||:|:::||.|:|.:||:||||.::.|:.|..|.|.|||.||||
 Frog  2288 GKHSAAADITSSLSEDQVPEAFLVMVLIQFGTMVVDRALYLRKTVMGKVIFQVILVFGIHFWMFF 2352

  Fly  1838 LVPYITSHSFGE-TAPVLFYLIKCLHMLLSAYQIRCGYPKRILGNVFTKGYSLANYIAFKIYMEI 1901
            ::|.:|...|.: |...|:|.:||::..|||||||||||.|:|||..||.|:..|...|:.:..:
 Frog  2353 ILPGVTERKFSQNTVAQLWYFVKCIYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLV 2417

  Fly  1902 PFLYILRTMLDWVCIDTTLTVMEWIKMEDIFQSVFIVRCYRQMDTDFPVLRGEPKALYSKLLIGG 1966
            |||..||.::|||..||||::..||.:|||:..:||::|:|:.:..:|..||:.|....|..:||
 Frog  2418 PFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGG 2482

  Fly  1967 TIILILIALIWSPLFLFALVGTV-GKPNIPQKADITVKINHYEPIYVSQSNSDILQ-FSNSDFQK 2029
            .||::||.::|.||...:|:.:| |..|.|....||:.:..|:||:...:..:.|| .::::|:.
 Frog  2483 MIIVLLICIVWFPLLFMSLIKSVAGVTNKPLDVSITITLGGYQPIFTMSAQQNQLQGLNDNEFKH 2547

  Fly  2030 LTNHIILDNYASDSMMLYDAVDVTAIKFYENSISLWNMPPPDK----ERLLHDLSNGETLDIHLT 2090
            |.|....:..|...:..|...|:|......||.|||.:.||.:    |||:.:  ...|..|..:
 Frog  2548 LHNIYKGNTPAMQFLESYMQEDITIAGLEGNSNSLWTISPPSRTMMIERLIQE--PDFTAVISWS 2610

  Fly  2091 LTLKCNLTPEAVIYETTYTLTENKVHTRDKLIRLMSNNFSNEKVIIPNILPKFI---------TV 2146
            :....:|..:|.:....||:: ....||.:|..:|:::..|:.|.:..|.|..:         .:
 Frog  2611 IRRNVSLGAKAEVTGEKYTIS-LAGQTRRELADMMNSSSPNKTVELIKIYPLCLKAPSDVTAKPI 2674

  Fly  2147 QRQQANAKFIKDYDGKFNRPIILTKIGKSQVY--WWEMNDYCNDRFYTEILSALPLSNCENGVVL 2209
            .:...|.:|:       |..::|.|...|:..  ||.:|     :.:.|.:.       ::|:.|
 Frog  2675 LQLLKNQEFV-------NISVLLRKENTSEGVREWWVVN-----QSHKEFIK-------KDGLEL 2720

  Fly  2210 YVFNDKSFPSAMSFLEKTGIIGLYTTFVYVVSRIIRSLIANNHRRIMFEDLPYVDRVLKLCTDIY 2274
            :||:||..|.::.||...||:|||.:.|.|:.:.:|...:.....||||:||.|||:|||||||:
 Frog  2721 WVFSDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIF 2785

  Fly  2275 LVRETQEYRLEEDLYGKLIFLYRSPETLIKWTRFKE 2310
            |||||.|..||||||.|||||||||||:|||||.|:
 Frog  2786 LVRETGELELEEDLYAKLIFLYRSPETMIKWTREKD 2821

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pzl	NP_001303493.1	PIEZO	<1201..1388	CDD:292539	55/264 (21%)
		Piezo_RRas_bdg	1901..2308	CDD:288978	148/423 (35%)
piezo2	XP_012820813.2	PIEZO	1357..1622	CDD:406366	59/295 (20%)
		Piezo_RRas_bdg	2416..2819	CDD:403405	148/424 (35%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	440	1.000	Domainoid score	I541
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1074	1.000	Inparanoid score	I247
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D13738at2759
OrthoFinder	1	1.000	-	-		FOG0001637
OrthoInspector	1	1.000	-	-		mtm9559
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1528
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	6	6.060