DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment NGEF and trio

DIOPT Version :9

Sequence 1:	NP_062824.2	Gene:	NGEF / 25791	HGNCID:	7807	Length:	710	Species:	Homo sapiens
Sequence 2:	NP_651960.2	Gene:	trio / 43974	FlyBaseID:	FBgn0024277	Length:	2263	Species:	Drosophila melanogaster

Alignment Length:	684	Identity:	138/684 - (20%)
Similarity:	255/684 - (37%)	Gaps:	220/684 - (32%)

- Green bases have known domain annotations that are detailed below.

Human    12 TRRKSASDQ-W-------NTDNEPAKVKPEL---------LPEKEETSQADQDIQDKEPHCH-IP 58
            |:|:..|.: |       .||..|:. ||.:         .|:|.....|:|  |:::|... ..
  Fly  1749 TKRRGFSGRNWLPLMNRKGTDKPPSS-KPLVKKPSEKNLRTPQKHAEELAEQ--QNQQPGASPAT 1810

Human    59 IKRNSIFNRSIRRKSKAKARD-NPERNASCLADSQDNGKSVNEPLTLNIPWSRMPPCRTAMQT-- 120
            :..:|.|..|.:....|.|.| .|:                 |.:.|.:|    ||.:...:.
  Fly  1811 VLPSSQFPSSTQHPGGAGAGDYEPD-----------------EEVGLELP----PPMKPIQEPHL 1854

Human   121 ----DPG-AQEMSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQ 180
                .|. |::..|||   ||.|:|.:   :..:|  |:|                ||:|
  Fly  1855 IANGPPAFAKDAKESS---GNMASPGK---MDGNP--LSE----------------IEEI----- 1890

Human   181 EYRDKSTLQEIETRRQQD--AEIED-NTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLL 242
               .|:|.::.|:..:.|  ..:|: :|||...|.|..:|......::..|
  Fly  1891 ---VKTTTEQHESNSRVDGGGGVENASTNGHGRSHDNNDESHTAVSDKAAA-------------- 1938

Human   243 SNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMF-ELVTSEASYYKSLNLLVSHFME--NER 304
                                         :|.::.:| ||:::|.:|.:.|:.:|:.:|.  |..
  Fly  1939 -----------------------------LKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNT 1974

Human   305 IRKILHPSE-----AHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYI 364
            ...|..|.:     ..::|:|:.|:......|.|......:::  .:|:..::.| :|..|::|.
  Fly  1975 NSDIPMPEDLKGGKMKLVFNNIKDIYEWHRDFFLRALRNCQKS--PADLGPLIKR-SATKFALYY 2036

Human   365 TYVSNQTYQE------RTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLV 423
            ||.||:...|      ..|...:::|...|              |..|:.:|.|.||||:.:||:
  Fly  2037 TYCSNKPLSEYIVSAHYQYFDSIRQKLGHR--------------LDLSNLIIKPVQRITKYELLI 2087

Human   424 QNILKRVEERS-ERECTAL-DAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISH 486
            :.|:|..|... .:|...| :|::::::|||..|         :.|:.::...:|..:
  Fly  2088 KEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVN---------DMMVVLRSLQDFDGE------- 2136

Human   487 SRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQ----VFDSAPRGL 547
               :..||.| .|.||........:| ..|:.:|||..:::.. .|...|.|    :|.......
  Fly  2137 ---ITAQGSL-LMQGPMNCVVDAAQK-HRELQVFLFQQIIIFA-DIEKTKNQYASPIFKYRSHIQ 2195

Human   548 LRVEELEDQGQTLANVFILRLLENADDR--EATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSR 610
            |...::::.|.....:      .:.|.:  |.|.:.:|:||.....|...|  |:
  Fly  2196 LNHMQMKELGDCRFQI------RSTDPKIPEMTIICQAASQENYAGWRDML--NK---------- 2242

Human   611 LLDCPQVQCVHPYVAQQPDELTLELADILNILDK 644
                         :.||.::|...|::.|:..:|
  Fly  2243 -------------ILQQQNDLIFMLSNPLSTKNK 2263

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
NGEF	NP_062824.2	Regulatory region, modulates activity toward RHOA, RAC1 and CDC42. /evidence=ECO:0000250	1..273		54/289 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..143		36/156 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	194..236		9/44 (20%)
		RhoGEF	277..456	CDD:279015	48/194 (25%)
		PH_ephexin	477..603	CDD:269929	26/131 (20%)
		PH	490..596	CDD:278594	24/111 (22%)
		SH3_ephexin1	616..670	CDD:212872	6/29 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	687..710
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103
		SPEC	563..778	CDD:238103
		SPEC	782..997	CDD:238103
		SPEC	897..1119	CDD:238103
		SPEC	1125..1226	CDD:197544
		RhoGEF	1287..1456	CDD:279015
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060
		PH	1495..1580	CDD:278594
		SH3_Kalirin_1	1643..1707	CDD:212786
		RhoGEF	1945..2122	CDD:279015	49/202 (24%)
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061	32/173 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3601
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.940