DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hspa5 and Hsp70Ab

DIOPT Version :9

Sequence 1:	NP_037215.1	Gene:	Hspa5 / 25617	RGDID:	2843	Length:	654	Species:	Rattus norvegicus
Sequence 2:	NP_524798.2	Gene:	Hsp70Ab / 44920	FlyBaseID:	FBgn0013276	Length:	642	Species:	Drosophila melanogaster

Alignment Length:	620	Identity:	378/620 - (60%)
Similarity:	486/620 - (78%)	Gaps:	14/620 - (2%)

- Green bases have known domain annotations that are detailed below.

  Rat    31 VGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDA 95
            :|||||||||||||:::|:|||||||||||.|||||||| :.||||||.||||:..||.||||||
  Fly     4 IGIDLGTTYSCVGVYQHGKVEIIANDQGNRTTPSYVAFT-DSERLIGDPAKNQVAMNPRNTVFDA 67

  Rat    96 KRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAY 160
            ||||||.::||.:.:|:|..|||||....||.|.|:. .|::|.|||||||:|||||||||||||
  Fly    68 KRLIGRKYDDPKIAEDMKHWPFKVVSDGGKPKIGVEY-KGESKRFAPEEISSMVLTKMKETAEAY 131

  Rat   161 LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKR-EGEKNILVFD 224
            ||:.:|.||:||||||||:|||||||||.||||||:||||||||||:||||||. :||:|:|:||
  Fly   132 LGESITDAVITVPAYFNDSQRQATKDAGHIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFD 196

  Rat   225 LGGGTFDVSLLTIDNG-VFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKL 288
            |||||||||:||||.| :|||.:|.|||||||||||.|::.|....:|:|..||:|.:.||:::|
  Fly   197 LGGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVTHLADEFKRKYKKDLRSNPRALRRL 261

  Rat   289 RREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKK 353
            |...|:|||.|||..:|.|||::.|||:||...::||:||||..||||:|::||:|.|.|:.:.|
  Fly   262 RTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEKALNDAKMDK 326

  Rat   354 SDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQD--TGDLVL 416
            ..|.:||||||||||||:|.|:::||:||..:..|||||||||||||||.:|||||.  ..|::|
  Fly   327 GQIHDIVLVGGSTRIPKVQSLLQDFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLL 391

  Rat   417 LDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGT 481
            :||.||:|||||.||||||||.||..:|.|:::.|||.:||||.|:|:||||||.:||||:.|||
  Fly   392 VDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTYADNQPGVSIQVYEGERAMTKDNNALGT 456

  Rat   482 FDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAE 546
            |||:||||||||||||||||::|.||||.|:|::..||....|||.||:.||:..||:||||:||
  Fly   457 FDLSGIPPAPRGVPQIEVTFDLDANGILNVSAKEMSTGKAKNITIKNDKGRLSQAEIDRMVNEAE 521

  Rat   547 KFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSPEDKETMEKAVEEKIEWLESHQDA 611
            |:|:||:|.::||.:||.||||.:::|..:  ::...|||...||.::.....:.|.||:|:..|
  Fly   522 KYADEDEKHRQRITSRNALESYVFNVKQAV--EQAPAGKLDEADKNSVLDKCNDTIRWLDSNTTA 584

  Rat   612 DIEDFKAKKKELEEIVQPIISKLY------GSGGP 640
            :.|:|..|.:||.....||::|::      |:|||
  Fly   585 EKEEFDHKLEELTRHCSPIMTKMHQQGAGAGAGGP 619

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hspa5	NP_037215.1	Required for interaction with KIAA1324. /evidence=ECO:0000250\|UniProtKB:P11021	1..80		39/48 (81%)
		HSPA5-like_NBD	28..403	CDD:212681	248/373 (66%)
		HSP70	30..635	CDD:278441	374/607 (62%)
		Nucleotide-binding (NBD). /evidence=ECO:0000250\|UniProtKB:P11021	125..280		111/156 (71%)
		Interdomain linker. /evidence=ECO:0000250\|UniProtKB:G3I8R9	409..419		3/11 (27%)
		Substrate-binding (SBD). /evidence=ECO:0000250\|UniProtKB:P11021	420..500		57/79 (72%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	631..654		5/16 (31%)
		Prevents secretion from ER	651..654
Hsp70Ab	NP_524798.2	PTZ00009	2..642	CDD:240227	378/620 (61%)
Hsp70Ab	NP_524798.2	HSPA1-2_6-8-like_NBD	3..379	CDD:212675	249/376 (66%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53894
OrthoDB	1	1.010	-	-		D288077at2759
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100083
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	7	6.730